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-<?xml version="1.0" encoding="UTF-8"?><rss xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:content="http://purl.org/rss/1.0/modules/content/" xmlns:atom="http://www.w3.org/2005/Atom" version="2.0"><channel><title><![CDATA[EPI2ME Labs's RSS Feed]]></title><description><![CDATA[Tutorials and workflows for nanopore sequencing.]]></description><link>https://labs.epi2me.io</link><generator>GatsbyJS</generator><lastBuildDate>Wed, 29 May 2024 16:41:19 GMT</lastBuildDate><item><title><![CDATA[Nanopore-only T2T assembly of a human genome]]></title><description><![CDATA[We present the only state-of-the-art assembly of a human genome using a single sequencing technology.]]></description><link>https://labs.epi2me.io/lc2024_t2t</link><guid isPermaLink="false">https://labs.epi2me.io/lc2024_t2t</guid><pubDate>Wed, 22 May 2024 00:00:00 GMT</pubDate><enclosure url="https://labs.epi2me.io/static/8756c974d21b4e9e0bcec008cabe1e84/32411/epi2me-t2t-assembly.png" length="0" type="image/png"/><content:encoded>&lt;p&gt;We are pleased to announce the release of a new addition to the Oxford Nanopore Open Data project as part of London Calling 2024: a Nanopore-only telomere-to-telomere (T2T) assembly dataset.
+<?xml version="1.0" encoding="UTF-8"?><rss xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:content="http://purl.org/rss/1.0/modules/content/" xmlns:atom="http://www.w3.org/2005/Atom" version="2.0"><channel><title><![CDATA[EPI2ME Labs's RSS Feed]]></title><description><![CDATA[Tutorials and workflows for nanopore sequencing.]]></description><link>https://labs.epi2me.io</link><generator>GatsbyJS</generator><lastBuildDate>Wed, 29 May 2024 16:48:01 GMT</lastBuildDate><item><title><![CDATA[Nanopore-only T2T assembly of a human genome]]></title><description><![CDATA[We present the only state-of-the-art assembly of a human genome using a single sequencing technology.]]></description><link>https://labs.epi2me.io/lc2024_t2t</link><guid isPermaLink="false">https://labs.epi2me.io/lc2024_t2t</guid><pubDate>Wed, 22 May 2024 00:00:00 GMT</pubDate><enclosure url="https://labs.epi2me.io/static/8756c974d21b4e9e0bcec008cabe1e84/32411/epi2me-t2t-assembly.png" length="0" type="image/png"/><content:encoded>&lt;p&gt;We are pleased to announce the release of a new addition to the Oxford Nanopore Open Data project as part of London Calling 2024: a Nanopore-only telomere-to-telomere (T2T) assembly dataset.
 This dataset was created using our new telomere-to-telomere (T2T) workflow, combining ultra-long reads, Pore-C and our new assembly-polishing chemistry to completely resolve haplotypes and achieve a state-of-the-art Q50 human assembly.
 To register your interest in the T2T workflow, &lt;a href=&quot;https://register.nanoporetech.com/telomere-to-telomere-bundle&quot;&gt;please click here&lt;/a&gt;. &lt;/p&gt;&lt;blockquote&gt;&lt;p&gt;The following cell line samples were obtained from the NIGMS Human Genetic Cell
 Repository at the Coriell Institute for Medical Research: GM24385&lt;/p&gt;&lt;/blockquote&gt;&lt;p&gt;This dataset contains the inputs and outputs of the T2T workflow, comprising basecalled reads from sequencing runs using four PromethION flow cells:&lt;/p&gt;&lt;ul&gt;&lt;li&gt;Two Ultra Long (SQK-ULK114) runs, basecalled using the model &lt;code&gt;[email protected]&lt;/code&gt;&lt;/li&gt;&lt;li&gt;One Pore-C (SQK-LSK114) run, basecalled with the model &lt;code&gt;[email protected]&lt;/code&gt;&lt;/li&gt;&lt;li&gt;One Assembly Polishing (SQK-APK114, using the 6b4 method) run, basecalled with a bespoke APK model that will be provided alongside the T2T bundle.&lt;/li&gt;&lt;/ul&gt;&lt;h3 id=&quot;data-location&quot;&gt;Data location&lt;/h3&gt;&lt;p&gt;As with previous releases the new dataset is available for anonymous download from