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Analysing ambient RNA background RNA contamination the soup with Seurat.utils

Abel Vertesy edited this page Sep 1, 2020 · 3 revisions

Using the script Soup.Analysis.of.ambient.RNA.R you can analyse the ambient RNA or soup content of your experiment

Fraction within the whole data

Absolute fraction soupProfile summarized-124719 barplot

Fraction within the Soup

Soup GEMs top Genes summarized-124719 barplot

Scatter plots highlighting Soup vs Cells differences

Soup VS Cells Av Exp GeneClasses 124719 Soup VS Cells Av Exp quantile 0 01 124719 Soup VS Cells Av Exp quantile 0 0025 124719


Top expressed genes

Soup GEMs top Genes-124719 barplot Soup GEMs top Genes non summarized-124719 barplot

PC mRNA in Cells

PC mRNA in Cells-124719 barplot