small RNA analysis of deep sequencing data. SeqBuster offers four tools to analyze all your small RNA data coming from Illumina sequencing.
- Installation
- adapter removal: remove 3' adapter
- miraligner: miRNA annotation using miRBase database
- R package isomiRs to visualize isomiRs and miRNA abundances in a group of samples (new)
- seqcluster analyzes the rest of small RNAs and annotate to multiple databases in bed format (new)
- SeqBuster team updates periodically the tools with more information about how to be used and new applications. If you have any comment please use: mailing-list. Also visit our blog or facebook or twitter to be up-to-day of the new information added in the wiki.
The development of Seqbuster was partly funded by the EU Seventh Framework Programme [FP7/2007-2013, grant number 262055, ESGI]. http://www.esgi-infrastructure.eu/#
SeqBuster is a bioinformatic tool for the processing and analysis of small RNAs datasets, reveals ubiquitous miRNA modifications in human embryonic cells. Pantano L, Estivill X, Martí E. Nucleic Acids Res. 2010 Mar;38(5):e34. Epub 2009 Dec 11.