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migrate in samtools, kraken, vcflib #1148
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4d079c8
migrate in kraken, vflib, samtools
martenson a0eb535
change shed.yml: remote repo url to iuc
martenson d9c1bf7
update the rest of the remote repo urls
martenson c345e50
set chunk size to 4 temporarily
martenson 2a298cb
fix samtools stats schema lint
martenson 70de720
remove tool_dependencies.xml for samtools, let conda do it
martenson b392dc3
samtools linting errors
yhoogstrate c5da921
samtools mpileup - attempt to get testing and linting working
yhoogstrate ae39c24
samtools mpileup - attempt 2 to get testing and linting working
yhoogstrate b637cef
samtools stats+sam_to_bam - small changes to get tests working
yhoogstrate eee57a1
Samtools: update to version_command
yhoogstrate 840024e
Samtools: update to version_command - attempt 2
yhoogstrate eaf496a
kraken: minor changes
yhoogstrate d99fc25
Kraken: removal of tool-dependencies
yhoogstrate 9c88880
samtools bam_to_cram small changes
yhoogstrate 223a8ac
add changes from @nsoranzo
martenson 990f27c
add more improvements by :eagle::eyes:
martenson 9cfe78d
typo breaking planemo test
yhoogstrate d0682b1
samtools bedcov: proper usage of multiple bam files
yhoogstrate 21b0a8f
updates test data of samtools flagstat and updates iterator in samtoo…
yhoogstrate ca83496
textual changes
yhoogstrate 8a279e5
kraken: more textual changes
yhoogstrate 8215ba7
samtools: small change
yhoogstrate 4dc44ce
samtools: a few more textual changes
yhoogstrate 361b7a9
samtoools: typo
yhoogstrate abc67ae
samtools idxstats: fix symlinking bai file
yhoogstrate cde9556
More changes thanks to Nicola
yhoogstrate a44101b
fix shedyaml to point at iuc repo
martenson 6e1b060
fix shedyaml to point at iuc repo
martenson 6c16017
fix shedyaml to point at iuc repo
martenson f8fb9de
update rmdup to samtools 1.3 and tiny improvements, all thanks to :ea…
martenson 9bb0b6d
Make test of cram/bam tools pass
yhoogstrate 5ce99d2
Fix more tests
bgruening 96d9878
test fixes
bgruening b758595
Change test data
bgruening 2f64c9d
removal of not used test files
yhoogstrate c339ded
upgraded mpileup tests
yhoogstrate 8e28b30
samtools reheader: allow testing with 1 line difference to correct fo…
yhoogstrate 9cf113c
samtools stats also reports stats on "N" and "0"
yhoogstrate 46b0568
samtools stats: moving test-data from sub-directory to allow properly…
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Introduction | ||
============ | ||
|
||
`Kraken <http://ccb.jhu.edu/software/kraken/>`__ is a taxonomic sequence | ||
classifier that assigns taxonomic labels to short DNA reads. It does | ||
this by examining the :math:`k`-mers within a read and querying a | ||
database with those :math:`k`-mers. This database contains a mapping of | ||
every :math:`k`-mer in | ||
`Kraken <http://ccb.jhu.edu/software/kraken/>`__'s genomic library to | ||
the lowest common ancestor (LCA) in a taxonomic tree of all genomes that | ||
contain that :math:`k`-mer. The set of LCA taxa that correspond to the | ||
:math:`k`-mers in a read are then analyzed to create a single taxonomic | ||
label for the read; this label can be any of the nodes in the taxonomic | ||
tree. `Kraken <http://ccb.jhu.edu/software/kraken/>`__ is designed to be | ||
rapid, sensitive, and highly precise. Our tests on various real and | ||
simulated data have shown | ||
`Kraken <http://ccb.jhu.edu/software/kraken/>`__ to have sensitivity | ||
slightly lower than Megablast with precision being slightly higher. On a | ||
set of simulated 100 bp reads, | ||
`Kraken <http://ccb.jhu.edu/software/kraken/>`__ processed over 1.3 | ||
million reads per minute on a single core in normal operation, and over | ||
4.1 million reads per minute in quick operation. | ||
|
||
The latest released version of Kraken will be available at the `Kraken | ||
website <http://ccb.jhu.edu/software/kraken/>`__, and the latest updates | ||
to the Kraken source code are available at the `Kraken GitHub | ||
repository <https://github.com/DerrickWood/kraken>`__. | ||
|
||
If you use `Kraken <http://ccb.jhu.edu/software/kraken/>`__ in your | ||
research, please cite the `Kraken | ||
paper <http://genomebiology.com/2014/15/3/R46>`__. Thank you! | ||
|
||
System Requirements | ||
=================== | ||
|
||
Note: Users concerned about the disk or memory requirements should read | ||
the paragraph about MiniKraken, below. | ||
|
||
- **Disk space**: Construction of Kraken's standard database will | ||
require at least 160 GB of disk space. Customized databases may | ||
require more or less space. Disk space used is linearly proportional | ||
to the number of distinct :math:`k`-mers; as of Feb. 2015, Kraken's | ||
default database contains just under 6 billion (6e9) distinct | ||
:math:`k`-mers. | ||
|
||
In addition, the disk used to store the database should be | ||
locally-attached storage. Storing the database on a network | ||
filesystem (NFS) partition can cause Kraken's operation to be very | ||
slow, or to be stopped completely. As NFS accesses are much slower | ||
than local disk accesses, both preloading and database building will | ||
be slowed by use of NFS. | ||
|
||
- **Memory**: To run efficiently, Kraken requires enough free memory to | ||
hold the database in RAM. While this can be accomplished using a | ||
ramdisk, Kraken supplies a utility for loading the database into RAM | ||
via the OS cache. The default database size is 75 GB (as of Feb. | ||
2015), and so you will need at least that much RAM if you want to | ||
build or run with the default database. | ||
|
||
- **Dependencies**: Kraken currently makes extensive use of Linux | ||
utilities such as sed, find, and wget. Many scripts are written using | ||
the Bash shell, and the main scripts are written using Perl. Core | ||
programs needed to build the database and run the classifier are | ||
written in C++, and need to be compiled using g++. Multithreading is | ||
handled using OpenMP. Downloads of NCBI data are performed by wget | ||
and in some cases, by rsync. Most Linux systems that have any sort of | ||
development package installed will have all of the above listed | ||
programs and libraries available. | ||
|
||
Finally, if you want to build your own database, you will need to | ||
install the | ||
`Jellyfish <http://www.cbcb.umd.edu/software/jellyfish/>`__ | ||
:math:`k`-mer counter. Note that Kraken only supports use of | ||
Jellyfish version 1. Jellyfish version 2 is not yet compatible with | ||
Kraken. | ||
|
||
- **Network connectivity**: Kraken's standard database build and | ||
download commands expect unfettered FTP and rsync access to the NCBI | ||
FTP server. If you're working behind a proxy, you may need to set | ||
certain environment variables (such as ``ftp_proxy`` or | ||
``RSYNC_PROXY``) in order to get these commands to work properly. | ||
|
||
- **MiniKraken**: To allow users with low-memory computing environments | ||
to use Kraken, we supply a reduced standard database that can be | ||
downloaded from the Kraken web site. When Kraken is run with a | ||
reduced database, we call it MiniKraken. | ||
|
||
The database we make available is only 4 GB in size, and should run | ||
well on computers with as little as 8 GB of RAM. Disk space required | ||
for this database is also only 4 GB. | ||
|
||
|
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categories: | ||
- Metagenomics | ||
description: Kraken for taxonomic designation. | ||
homepage_url: http://ccb.jhu.edu/software/kraken/ | ||
long_description: | | ||
Kraken is a system for assigning taxonomic labels to short DNA | ||
sequences, usually obtained through metagenomic studies. Previous attempts by other | ||
bioinformatics software to accomplish this task have often used sequence alignment | ||
or machine learning techniques that were quite slow, leading to the development | ||
of less sensitive but much faster abundance estimation programs. Kraken aims to | ||
achieve high sensitivity and high speed by utilizing exact alignments of k-mers | ||
and a novel classification algorithm. | ||
name: kraken | ||
owner: devteam | ||
remote_repository_url: https://github.com/galaxyproject/tools-iuc/blob/master/tool_collections/kraken/kraken/ | ||
type: unrestricted |
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../README.rst |
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<?xml version="1.0"?> | ||
<tool id="kraken" name="Kraken" version="1.2.1"> | ||
<description> | ||
assign taxonomic labels to sequencing reads | ||
</description> | ||
<macros> | ||
<import>macros.xml</import> | ||
</macros> | ||
<expand macro="requirements" /> | ||
<expand macro="version_command" /> | ||
<command detect_errors="exit_code"> | ||
<![CDATA[ | ||
@SET_DATABASE_PATH@ && | ||
|
||
kraken | ||
--threads \${GALAXY_SLOTS:-1} | ||
@INPUT_DATABASE@ | ||
${only_classified_output} | ||
|
||
#if str( $quick_operation.quick ) == "yes": | ||
--quick | ||
--min-hits ${quick_operation.min_hits} | ||
|
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#end if | ||
|
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#if $single_paired.single_paired_selector == 'yes' | ||
#if $forward_input.is_of_type( 'fastq' ): | ||
--fastq-input | ||
#else: | ||
--fasta-input | ||
#end if | ||
'${single_paired.forward_input}' '${single_paired.reverse_input}' | ||
${single_paired.check_names} | ||
#elif $single_paired.single_paired_selector == "collection": | ||
#if $single_paired.input_pair.forward.is_of_type( 'fastq' ): | ||
--fastq-input | ||
#else: | ||
--fasta-input | ||
#end if | ||
"${single_paired.input_pair.forward}" "${single_paired.input_pair.reverse}" | ||
${single_paired.check_names} | ||
#else: | ||
#if $single_paired.input_sequences.is_of_type('fastq') | ||
--fastq-input | ||
#else: | ||
--fasta-input | ||
#end if | ||
'${single_paired.input_sequences}' | ||
#end if | ||
|
||
#if $split_reads: | ||
--classified-out "${classified_out}" --unclassified-out "${unclassified_out}" | ||
#end if | ||
|
||
## The --output option was changed to redirect as it does not work properly is some situations. For example, on test database the tool classifies 4 reads but does not write them into a file if --output is specified. It does however print correct output into STDOUT. This behavior can be re-created with test database provided in test-data/test_db/ folder. This is the reason for incrementing version number from 1.1.2 to 1.1.3 | ||
|
||
> "${output}" | ||
##kraken-translate --db ${kraken_database.fields.name} "${output}" > "${translated}" | ||
]]></command> | ||
<inputs> | ||
<conditional name="single_paired"> | ||
<param name="single_paired_selector" type="select" label="Single or paired reads" help="--paired"> | ||
<option value="collection">Collection</option> | ||
<option value="yes">Paired</option> | ||
<option selected="True" value="no">Single</option> | ||
</param> | ||
<when value="collection"> | ||
<param format="fasta,fastq" name="input_pair" type="data_collection" collection_type="paired" label="Collection of paired reads" help="FASTA or FASTQ datasets" /> | ||
<param name="check_names" argument="--check-names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match"/> | ||
</when> | ||
<when value="yes"> | ||
<param format="fasta,fastq" name="forward_input" type="data" label="Forward strand" help="FASTA or FASTQ dataset"/> | ||
<param format="fasta,fastq" name="reverse_input" type="data" label="Reverse strand" help="FASTA or FASTQ dataset"/> | ||
<param name="check_names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match" help="--check-names" /> | ||
</when> | ||
<when value="no"> | ||
<param format="fasta,fastq" label="Input sequences" name="input_sequences" type="data" help="FASTA or FASTQ datasets"/> | ||
</when> | ||
|
||
</conditional> | ||
<param name="split_reads" type="boolean" label="Output classified and unclassified reads?" help="Sets --unclassified-out and --classified-out"/> | ||
|
||
<conditional name="quick_operation"> | ||
<param argument="--quick" type="select" label="Enable quick operation" | ||
help="Quick mode: rather than searching all k-mers in a sequence, stop classification after a specified number of database hit"> | ||
<option value="yes">Yes</option> | ||
<option selected="True" value="no">No</option> | ||
</param> | ||
<when value="yes"> | ||
<param name="min_hits" argument="--min-hits" type="integer" value="1" label="Number of hits required for classification" | ||
help="min-hits will allow you to require multiple hits before declaring a sequence classified, which can be especially useful with custom databases when testing to see if sequences either do or do not belong to a particular genome; default=1"/> | ||
</when> | ||
<when value="no"/><!-- Do absolutely nothing --> | ||
</conditional> | ||
|
||
<param name="only_classified_output" argument="--only-classified-output" type="boolean" checked="False" truevalue="--only-classified-output" falsevalue="" | ||
label="Print no Kraken output for unclassified sequences"/> | ||
|
||
<expand macro="input_database" /> | ||
</inputs> | ||
<outputs> | ||
<data name="classified_out" format_source="input_sequences" label="${tool.name} on ${on_string}: Classified reads"> | ||
<filter>(split_reads)</filter> | ||
</data> | ||
<data name="unclassified_out" format_source="input_sequences" label="${tool.name} on ${on_string}: Unclassified reads"> | ||
<filter>(split_reads)</filter> | ||
</data> | ||
<data name="output" format="tabular" label="${tool.name} on ${on_string}: Classification"/> | ||
<!--<data format="tabular" label="${tool.name} on ${on_string}: Translated classification" name="translated" />--> | ||
</outputs> | ||
|
||
<tests> | ||
<test> | ||
<param name="single_paired_selector" value="no"/> | ||
<param name="input_sequences" value="kraken_test1.fa" ftype="fasta"/> | ||
<param name="split_reads" value="false"/> | ||
<param name="quick" value="no"/> | ||
<param name="only-classified-output" value="false"/> | ||
<param name="kraken_database" value="test_db"/> | ||
|
||
<output name="output" file="kraken_test1_output.tab" ftype="tabular"/> | ||
</test> | ||
</tests> | ||
<help> | ||
<![CDATA[ | ||
**What it does** | ||
|
||
Kraken is a taxonomic sequence classifier that assigns taxonomic labels to short DNA reads. It does this by examining the k-mers within a read and querying a database with those k-mers. This database contains a mapping of every k-mer in Kraken's genomic library to the lowest common ancestor (LCA) in a taxonomic tree of all genomes that contain that k-mer. The set of LCA taxa that correspond to the k-mers in a read are then analyzed to create a single taxonomic label for the read; this label can be any of the nodes in the taxonomic tree. Kraken is designed to be rapid, sensitive, and highly precise. | ||
|
||
----- | ||
|
||
**Output Format** | ||
|
||
Each sequence classified by Kraken results in a single line of output. Output lines contain five tab-delimited fields; from left to right, they are:: | ||
|
||
1. "C"/"U": one letter code indicating that the sequence was either classified or unclassified. | ||
2. The sequence ID, obtained from the FASTA/FASTQ header. | ||
3. The taxonomy ID Kraken used to label the sequence; this is 0 if the sequence is unclassified. | ||
4. The length of the sequence in bp. | ||
5. A space-delimited list indicating the LCA mapping of each k-mer in the sequence. For example, "562:13 561:4 A:31 0:1 562:3" would indicate that: | ||
a) the first 13 k-mers mapped to taxonomy ID #562 | ||
b) the next 4 k-mers mapped to taxonomy ID #561 | ||
c) the next 31 k-mers contained an ambiguous nucleotide | ||
d) the next k-mer was not in the database | ||
e) the last 3 k-mers mapped to taxonomy ID #562 | ||
]]> | ||
</help> | ||
<expand macro="citations" /> | ||
</tool> |
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../macros.xml |
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>gi|145231|gb|M33724.1|ECOALPHOA Escherichia coli K-12 truncated PhoA (phoA) gene, partial cds; and transposon Mu dI, partial sequence | ||
CAAAGCTCCGGGCCTCACCCAGGCGCTAAATACCAAAGATGGCGCAGTGATGGTGATGAGTTACGGGAAC | ||
TCCGAAGAGGATTCACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCA | ||
ATGAAGCGGCGCACGAAAAACGCGAAAGCGT | ||
|
||
>gi|145232|gb|M33725.1|ECOALPHOB Escherichia coli K12 phoA pseudogene and transposon Mu dl-R, partial sequence | ||
CTGTCATAAAGTTGTCACGGCCGAGACTTATAGTCGCTTTGTTTTTATTTTTTAATGTATTTGTACATGG | ||
AGAAAATAAAGTGAAACAAAGCACTATTGCACTGGCACTCTTACCGTTACTGTTTACCCCTGTGACAAAA | ||
GCCCGGACACCAGTGAAGCGGCGCACGAAAAACGCGAAAGCGT | ||
|
||
>gi|145234|gb|M33727.1|ECOALPHOE Escherichia coli K12 upstream sequence of psiA5::Mu dI. is identical to psiA30 upstream sequence; putative (phoA) pseudogene and transposon Mu dl-R, partial sequence | ||
TTGTTTTTATTTTTTAATGTATTTGTACATGGAGAAAATAAAGTGAAACAAAGCACTATTGCACTGGTGA | ||
AGCGGCGCACGAAAAACGCGAAAGCGT | ||
|
||
>gi|146195|gb|J01619.1|ECOGLTA Eschericia coli gltA gene, sdhCDAB operon and sucABCD operons, complete sequence | ||
GAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTC | ||
ACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCAAAATAATACTTATTTATAA | ||
TCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCA | ||
TCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACC | ||
TAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTG | ||
TTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAAACTTTGGATACC | ||
GGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAT | ||
TGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTC | ||
TGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCA | ||
GCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTCCTTTAAACATAA | ||
CAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTT | ||
TTAGTTTTGCTTAAAAAATATTGTTAGTTTTATTAAATTGGAAAACTAAATTATTGGTATCATGAATTGT | ||
TGTATGATGATAAATATAGGGGGGATATGATAGACGTCATTTTCATAGGGTTATAAAATGCGACTACCAT | ||
GAAGTTTTTAATTCAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATA | ||
GGTCTGTTAATGGATTTTATTTTTACAAGTTTTTTGTGTTTAGGCATATAAAAATCAAGCCCGCCATATG | ||
AACGGCGGGTTAAAATATTTACAACTTAGCAATCGAACCATTAACGCTTGATATCGCTTTTAAAGTCGCG | ||
TTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAG | ||
TGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCG | ||
CTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTT | ||
CAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCACT | ||
TCATGGCAGGTTTCACGCATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCA | ||
TCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAAAAAATTCCGGAATGTGTTTAACGGAGCTGAT | ||
TTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCT | ||
GCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCT | ||
GTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGG | ||
TTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACA | ||
AATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACG | ||
CGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGT | ||
AATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCG | ||
TGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCAT | ||
TCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTG | ||
CAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCT | ||
GGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTT | ||
TCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGG | ||
TCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAC | ||
CCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCA | ||
GGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAACTTGTC | ||
TACCACTAATAACTGTCCCGAATGAATTGGTCAATACTCCACACTGTTACATAAGTTAATCTTAGGTGAA | ||
ATACCGACTTCATAACTTTTACGCATTATATGCTTTTCCTGGTAATGTTTGTAACAACTTTGTTGAATGA | ||
TTGTCAAATTAGATGATTAAAAATTAAATAAATGTTGTTATCGTGACCTGGATCACTGTTCAGGATAAAA | ||
CCCGACAAACTATATGTAGGTTAATTGTAATGATTTTGTGAACAGCCTATACTGCCGCCAGTCTCCGGAA | ||
CACCCTGCAATCCCGAGCCACCCAGCGTTGTAACGTGTCGTTTTCGCATCTGGAAGCAGTGTTTTGCATG | ||
ACGCGCAGTTATAGAAAGGACGCTGTCTGACCCGCAAGCAGACCGGAGGAAGGAAATCCCGACGTCTCCA | ||
GGTAACAGAAAGTTAACCTCTGTGCCCGTAGTCCCCAGGGAATAATAAGAACAGCATGTGGGCGTTATTC | ||
ATGATAAGAAATGTGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGG | ||
CGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCT | ||
TCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTC | ||
AAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGA | ||
TGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTAC | ||
TGTCGTGCTTTCACTTCTCGCAGGAGTCCTCGTATGGTAAGCAACGCCTCCGCATTAGGACGCAATGGCG | ||
TACATGATTTCATCCTCGTTCGCGCTACCGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTT | ||
TTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTG | ||
TTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACT | ||
ACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAT | ||
TTATGGATTCGTTGTGGTGTGGGGTGTGTGATGAAATTGCCAGTCAGAGAATTTGATGCAGTTGTGATTG |
4 changes: 4 additions & 0 deletions
4
tool_collections/kraken/kraken/test-data/kraken_test1_output.tab
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C gi|145231|gb|M33724.1|ECOALPHOA 83333 171 83333:162 | ||
C gi|145232|gb|M33725.1|ECOALPHOB 83333 183 83333:174 | ||
C gi|145234|gb|M33727.1|ECOALPHOE 83333 97 83333:88 | ||
C gi|146195|gb|J01619.1|ECOGLTA 83333 3850 83333:3841 |
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../../test_database.loc |
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../../../database.idx |
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../../../database.kdb |
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tool_collections/kraken/kraken/test-data/test_db/taxonomy/names.dmp
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../../../../names.dmp |
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tool_collections/kraken/kraken/test-data/test_db/taxonomy/nodes.dmp
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../../../../nodes.dmp |
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tool_collections/kraken/kraken/tool-data/kraken_databases.loc.sample
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../../kraken_databases.loc.sample |
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../tool_data_table_conf.xml.sample |
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../tool_data_table_conf.xml.test |
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@martenson The kraken wrappers are under devteam and the others under IUC. Is it ok to choose either devteam or IUC for all of them?
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@yhoogstrate I do not understand the question. Please rephrase.
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@martenson, oops I think I clicked at the wrong file to explain myself. The following .shed.yml files have their
remote_repository_url:
linked totools-devteam
:While all other tools have this linked to
tools-iuc
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I'm pretty sure these are just leftovers, all
remote_repository_url
should link totools-iuc
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@yhoogstrate I think @nsoranzo is right. I fixed those 3.