All notable changes to this project will be documented in this file.
The format is based on Keep a Changelog, and this project adheres to Semantic Versioning.
- Updated recommended (32GB) and minimum (16GB, with
--assembly false
) memory requirements.
- Reconcile workflow with wf-template v5.2.6.
- Reconcile workflow with wf-template v5.2.5.
- Show Nextclade version in software versions table.
- Nextclade typing for both reference-based and optional de novo consensus assemblies.
- Clade I reference sequence (
NC_003310.1
) is now available for selection.
- Default reference sequence changed to
NC_003310.1
(Clade I) following mpox PHEIC announcement.
- Support for uBAM (unaligned BAM) input files.
--override_basecaller_cfg
parameter for cases where automatic basecall model detection fails or users wish to override the automatic choice.
- Now checks if input BAM files have been aligned against the selected reference.
- Updated Medaka to v1.12.0.
- The
--medaka_options
parameter as the appropriate Medaka model is now automatically determined from the input data.
- Workflow can now take either a BAM file or FASTQ file as input.
- Improved Medaka speed on ARM processors.
- Updated documentation format.
- Parameter to turn off assembly has been fixed.
per-read-stats.tsv
is now produced per sample and compressed to<sample_alias>.per-read-stats.tsv.gz
- Bumped minimum required Nextflow version to 23.04.2.
- Enum choices are enumerated in the
--help
output. - Enum choices are enumerated as part of the error message when a user has selected an invalid choice.
- Updated GitHub issue templates to force capture of more information.
- Example command to use demo data.
- Spaces in sample aliases are now replaced with underscores.
- Deprecated parameter
process_label
.
- Updated whole workflow to bring up-to-date with recent template changes
- Configuration for running demo data in AWS
- Updated description in manifest
- conda support
- Updating templates
- Documentation
- Fix for error with empty VCF files (@MarieLataretu)
- Minimum coverage parameter
- Args parser for fastqingress
- Set out_dir option type to ensure output is written to correct directory on Windows
- Tidied up docs
- Restricting assembly to only reads that map to mpx
First release