Running Fusera

Access the help with fusera -h:

NAME:
   fusera - A FUSE interface to the NCBI Sequence Read Archive (SRA)

USAGE:
   fusera <command> [<flags>] mountpoint
   
VERSION:
   0.0.-beta
   
COMMANDS:
  mount, m  to mount a folder

    FLAGS:
      --ngc value       path to file that authenticates access [$DBGAP_CREDENTIALS]
      --acc value       comma separated list of accessions [$DBGAP_ACC]
      --acc-file value  path to a cart file, listing accession numbers [$DBGAP_ACCFILE]
      --loc value       preferred region [$DBGAP_LOC]
      --debug           Enable debugging output.

  unmount, u  to unmount a folder

    FLAGS:
      --debug  Enable debugging output.

MISC OPTIONS:
   --help, -h     Print this help text and exit successfully.
   --version, -v  print the version

A simple run of Fusera:

$ fusera mount --ngc [path/to/ngcfile] --acc [comma separated list of SRR#s] --loc [s3.us-east-1|gs.US] <mountpoint>

All of these flags also have equivalent environment variables ($DBGAP_CREDENTIALS, $DBGAP_ACC, $DBGAP_LOC, etc), which might be more handy when attempting to automate the use of fusera over multiple machines or reduce redundancy if you find yourself consistently invoking fusera with the same flags. Using all the environment variables, a call to fusera could look like so:

$ fusera mount ~/studies

If and only if you are running fusera or sracp on an AWS instance, both of these tools can resolve the location flag for you. If this flag is left off of either of these tools and you are not on an AWS instance, prepare for a long wait for a timeout and then a disappointing error informing you that you forgot the location flag.

The <mountpoint> must be an existing, empty directory, to which the user has read and write permissions.

It is recommended that the mountpoint be a directory owned by the user. Creating the mountpoint in system directories such as /mnt, /tmp have special uses in unix systems and should be avoided.

Because of the nature of FUSE systems, only the user who ran fusera will be able to read the files mounted. This can be changed by editing a config file (reference) on the machine to allow_others, but be warned that there are security implications to be considered: https://github.com/libfuse/libfuse#security-implications.

Accessions can be specified through the commmand line using the --acc flag, or, by reference to a file with space or comma separated accessions using the --acc-file option. The union of these two sets of accessions is used to build the FUSE file system, with duplicates eliminated.

If the you want to run fusera in the background you can do so with shell commands. Example:

fusera ~/tmp > output.log  2>&1 &
[1] 12464
disown %1

Breakdown: > output.log This redirects stdout to a file named output.log 2>&1 The way to redirect stderr to print with stdout so it is caught in output.log as well & Run this process in the background so I can continue using the shell disown %1 This will keep fusera running even if the terminal you ran it from is closed. The %1 indicates you want to disown the first thing you started running in the background, hence the 1 in brackets that gets printed after running fusera this way: [1] 12464. If your terminal prints a different number in the brackets, use it instead.