diff --git a/topics/genome-annotation/tutorials/lncrna/tutorial.md b/topics/genome-annotation/tutorials/lncrna/tutorial.md index e18e1672b6f7c9..74ee93de03f68c 100644 --- a/topics/genome-annotation/tutorials/lncrna/tutorial.md +++ b/topics/genome-annotation/tutorials/lncrna/tutorial.md @@ -109,7 +109,7 @@ A reference annotation file in GTF or GFF3 format can be provided to StringTie w > Transcripts assembly > -> {% tool [StringTie](toolshed.g2.bx.psu.edu/repos/iuc/stringtie/stringtie/2.1.7+galaxy1) %} with the following parameters: +> {% tool [StringTie](toolshed.g2.bx.psu.edu/repos/iuc/stringtie/stringtie/2.2.3+galaxy1) %} with the following parameters: > - *"Input options"*: `Short reads` > - {% icon param-file %} *"Input short mapped reads"*: `SRR8534859_RNASeq_mapped.bam` > - *"Specify strand information"*: Unstranded @@ -132,7 +132,7 @@ We obtain an annotation file (GTF format) which contained all assembled transcri > > > > > -> > Specific features can be extracted from the GTF file using for example {% tool [Extract features from GFF data](Extract_features1) %}. By selecting `transcript` From `column 3 / Feature`, we can select only the transcript elements present in this annotation file. Assembly contains 14,877 transcripts (corresponding to the number of lines in the filtered GTF file). +> > Specific features can be extracted from the GTF file using for example {% tool [Extract features from GFF data](Extract_features1) %}. By selecting `transcript` From `column 3 / Feature`, we can select only the transcript elements present in this annotation file. Assembly contains 17,653 transcripts (corresponding to the number of lines in the filtered GTF file). > > > {: .solution} > @@ -146,11 +146,11 @@ To use FEELnc, we need to have a reference annotation file in GTF format, which > FEELnc > -> 1. {% tool [gffread](toolshed.g2.bx.psu.edu/repos/devteam/gffread/gffread/2.2.1.3+galaxy0) %} with the following parameters: +> 1. {% tool [gffread](toolshed.g2.bx.psu.edu/repos/devteam/gffread/gffread/2.2.1.4+galaxy0) %} with the following parameters: > - {% icon param-file %} *"Input BED, GFF3 or GTF feature file"*: `genome_annotation.gff3` > - *"Feature File Output"*: `GTF` > -> 2. {% tool [FEELnc](toolshed.g2.bx.psu.edu/repos/iuc/feelnc/feelnc/0.2) %} with the following parameters: +> 2. {% tool [FEELnc](toolshed.g2.bx.psu.edu/repos/iuc/feelnc/feelnc/0.2.1) %} with the following parameters: > - {% icon param-file %} *"Transcripts assembly"*: `Assembled transcript` (output of **StringTie** {% icon tool %}) > - {% icon param-file %} *"Reference annotation"*: `genome_annotation.gtf` (Output of **gffread** {% icon tool %}) > - {% icon param-file %} *"Genome sequence"*: `genome_assembly.fasta` @@ -186,8 +186,8 @@ For future analyses, it would be interesting to use an updated annotation contai > Merge the annotations > -> {% tool [concatenate](toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1) %} with the following parameters: -> - {% icon param-file %} *"Datasets to concatenate"*: `genome_annotation.gtf` +> {% tool [concatenate](toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/1.0.0) %} with the following parameters: +> - {% icon param-file %} *"Datasets to concatenate"*: `genome_annotation.gtf` (Output of **gffread** {% icon tool %}) > - Insert Dataset > - {% icon param-file %} *"Dataset"*: `lncRNA annotation with FEELnc` >