diff --git a/lib/galaxy/config/sample/datatypes_conf.xml.sample b/lib/galaxy/config/sample/datatypes_conf.xml.sample
index acc0c55da20f..41e9a3259b9f 100644
--- a/lib/galaxy/config/sample/datatypes_conf.xml.sample
+++ b/lib/galaxy/config/sample/datatypes_conf.xml.sample
@@ -202,6 +202,7 @@
     <datatype extension="grib" type="galaxy.datatypes.binary:Grib" mimetype="application/octet-stream" display_in_upload="true"/>
     <datatype extension="loom" type="galaxy.datatypes.binary:Loom" description="An HDF5-based Loom File" mimetype="application/octet-stream" display_in_upload="true"/>
     <datatype extension="h5ad" type="galaxy.datatypes.binary:Anndata" description="An HDF5-based anndata File" mimetype="application/octet-stream" display_in_upload="true"/>
+    <datatype extension="h5mu" type="galaxy.datatypes.binary:H5" mimetype="application/octet-stream" display_in_upload="true" subclass="true" description="MuData is a format analogous to Anndata for multimodal datasets and is based on HDF5" description_url="https://github.com/scverse/mudata"/>
     <datatype extension="visium.tar.gz" type="galaxy.datatypes.binary:Visium" subclass="true" display_in_upload="true" description="Visium is a tar.gz archive with at least a 'Spatial' subfolder, a filtered h5 file and a raw h5 file." />
     <datatype extension="mz5" type="galaxy.datatypes.binary:H5" subclass="true" mimetype="application/octet-stream" display_in_upload="true"/>
     <datatype extension="hyphy_results.json" type="galaxy.datatypes.text:Json" mimetype="application/json" subclass="true" display_in_upload="false"/>