diff --git a/pkg/linter/test/rules/always_specify_types_test.dart b/pkg/linter/test/rules/always_specify_types_test.dart
index f5d2cef2d49b..d6570233da28 100644
--- a/pkg/linter/test/rules/always_specify_types_test.dart
+++ b/pkg/linter/test/rules/always_specify_types_test.dart
@@ -270,7 +270,7 @@ class C {
     await assertNoDiagnostics(r'''
 class S {
   S({int? p1, required int p2});
-}  
+}
 class C extends S {
   C({super.p1, required super.p2});
 }
diff --git a/pkg/linter/test/rules/annotate_redeclares_test.dart b/pkg/linter/test/rules/annotate_redeclares_test.dart
index 6c2628320828..0d3dbcd6a84d 100644
--- a/pkg/linter/test/rules/annotate_redeclares_test.dart
+++ b/pkg/linter/test/rules/annotate_redeclares_test.dart
@@ -82,7 +82,7 @@ extension type E(A a) implements A {
 
   test_method_annotated() async {
     await assertNoDiagnostics(r'''
-import 'package:meta/meta.dart';   
+import 'package:meta/meta.dart';
 class A {
   void m() {}
 }
diff --git a/pkg/linter/test/rules/avoid_classes_with_only_static_members_test.dart b/pkg/linter/test/rules/avoid_classes_with_only_static_members_test.dart
index b5f84a9ad3ed..24e9cad5d785 100644
--- a/pkg/linter/test/rules/avoid_classes_with_only_static_members_test.dart
+++ b/pkg/linter/test/rules/avoid_classes_with_only_static_members_test.dart
@@ -30,7 +30,7 @@ class A {
     var b = newFile('$testPackageLibPath/b.dart', r'''
 part of 'a.dart';
 
-augment class A { 
+augment class A {
   int a = 1;
 }
 ''');
@@ -52,7 +52,7 @@ class A {}
     var b = newFile('$testPackageLibPath/b.dart', r'''
 part of 'a.dart';
 
-augment class A { 
+augment class A {
   static int f = 1;
 }
 ''');
@@ -76,7 +76,7 @@ class A {}
     var b = newFile('$testPackageLibPath/b.dart', r'''
 part of 'a.dart';
 
-augment class A { 
+augment class A {
   static void m() {}
 }
 ''');
diff --git a/pkg/linter/test/rules/avoid_field_initializers_in_non_const_classes_test.dart b/pkg/linter/test/rules/avoid_field_initializers_in_non_const_classes_test.dart
index 20631bdbd88a..ee72cc0c2e4f 100644
--- a/pkg/linter/test/rules/avoid_field_initializers_in_non_const_classes_test.dart
+++ b/pkg/linter/test/rules/avoid_field_initializers_in_non_const_classes_test.dart
@@ -30,7 +30,7 @@ class A {
     var b = newFile('$testPackageLibPath/b.dart', r'''
 part of 'a.dart';
 
-augment class A { 
+augment class A {
   A.aa() : a = 1;
 }
 ''');
@@ -105,7 +105,7 @@ augment class A {
     newFile('$testPackageLibPath/a.dart', r'''
 part 'test.dart';
 
-class A { 
+class A {
   A.aa();
 }
 ''');
@@ -124,7 +124,7 @@ augment class A {
     newFile('$testPackageLibPath/a.dart', r'''
 part 'test.dart';
 
-class A { 
+class A {
   const A();
 }
 ''');
diff --git a/pkg/linter/test/rules/avoid_renaming_method_parameters_test.dart b/pkg/linter/test/rules/avoid_renaming_method_parameters_test.dart
index d11ff1366571..d34980790d49 100644
--- a/pkg/linter/test/rules/avoid_renaming_method_parameters_test.dart
+++ b/pkg/linter/test/rules/avoid_renaming_method_parameters_test.dart
@@ -57,7 +57,7 @@ part of 'a.dart';
 
 augment class A {
   augment void m(int q) {}
-  augment void m(int q) {} 
+  augment void m(int q) {}
 }
 ''', [
       lint(58, 1), // Only the first augmentation gets linted.
diff --git a/pkg/linter/test/rules/overridden_fields_test.dart b/pkg/linter/test/rules/overridden_fields_test.dart
index 3c8203fb3a4e..43d66cb9d5ce 100644
--- a/pkg/linter/test/rules/overridden_fields_test.dart
+++ b/pkg/linter/test/rules/overridden_fields_test.dart
@@ -31,7 +31,7 @@ class A extends O { }
     var b = newFile('$testPackageLibPath/b.dart', r'''
 part of 'a.dart';
 
-augment class A { 
+augment class A {
   final a = '';
 }
 ''');
@@ -41,7 +41,7 @@ augment class A {
 
     result = await resolveFile(b.path);
     await assertDiagnosticsIn(errors, [
-      lint(46, 1),
+      lint(45, 1),
     ]);
   }
 
@@ -54,7 +54,7 @@ class O {
 }
 
 class A extends O {
-  @override 
+  @override
   final a = '';
 }
 ''');
@@ -62,14 +62,14 @@ class A extends O {
     var b = newFile('$testPackageLibPath/b.dart', r'''
 part of 'a.dart';
 
-augment class A { 
+augment class A {
   augment final a = '';
 }
 ''');
 
     result = await resolveFile(a.path);
     await assertDiagnosticsIn(errors, [
-      lint(86, 1),
+      lint(85, 1),
     ]);
 
     result = await resolveFile(b.path);
diff --git a/pkg/linter/test/rules/prefer_void_to_null_test.dart b/pkg/linter/test/rules/prefer_void_to_null_test.dart
index eae8d0ce3eea..398c9f5ecd81 100644
--- a/pkg/linter/test/rules/prefer_void_to_null_test.dart
+++ b/pkg/linter/test/rules/prefer_void_to_null_test.dart
@@ -23,7 +23,7 @@ part 'test.dart';
 
 class A {
   Future<Null>? f;
-}  
+}
 ''');
 
     await assertNoDiagnostics(r'''
@@ -55,7 +55,7 @@ part 'test.dart';
 
 class A {
   Future<Null>? get v => null;
-}  
+}
 ''');
 
     await assertNoDiagnostics(r'''
diff --git a/pkg/linter/tool/checks/driver.dart b/pkg/linter/tool/checks/driver.dart
index 4caeb9398d0b..64447836dff9 100644
--- a/pkg/linter/tool/checks/driver.dart
+++ b/pkg/linter/tool/checks/driver.dart
@@ -22,6 +22,7 @@ import 'package:path/path.dart' as path;
 
 import '../../test/mocks.dart';
 import 'rules/no_solo_tests.dart';
+import 'rules/no_trailing_spaces.dart';
 import 'rules/visit_registered_nodes.dart';
 
 Future<void> main() async {
@@ -31,7 +32,11 @@ Future<void> main() async {
   }
 }
 
-var customChecks = [VisitRegisteredNodes(), NoSoloTests()];
+var customChecks = [
+  VisitRegisteredNodes(),
+  NoSoloTests(),
+  NoTrailingSpaces(),
+];
 
 Future<List<AnalysisError>> runChecks() async {
   var rules =
diff --git a/pkg/linter/tool/checks/rules/no_trailing_spaces.dart b/pkg/linter/tool/checks/rules/no_trailing_spaces.dart
new file mode 100644
index 000000000000..42a566139403
--- /dev/null
+++ b/pkg/linter/tool/checks/rules/no_trailing_spaces.dart
@@ -0,0 +1,54 @@
+// Copyright (c) 2024, the Dart project authors. Please see the AUTHORS file
+// for details. All rights reserved. Use of this source code is governed by a
+// BSD-style license that can be found in the LICENSE file.
+
+import 'package:analyzer/dart/ast/ast.dart';
+import 'package:analyzer/dart/ast/visitor.dart';
+import 'package:linter/src/analyzer.dart';
+
+const _desc = r"Don't create string literals with trailing spaces in tests.";
+
+class NoTrailingSpaces extends LintRule {
+  static const LintCode code = LintCode('no_trailing_spaces', _desc,
+      correctionMessage: 'Try removing the trailing spaces.',
+      hasPublishedDocs: true);
+
+  NoTrailingSpaces()
+      : super(
+          name: 'no_trailing_spaces',
+          description: _desc,
+        );
+
+  @override
+  LintCode get lintCode => code;
+
+  @override
+  void registerNodeProcessors(
+      NodeLintRegistry registry, LinterContext context) {
+    if (context.definingUnit.unit.inTestDir) {
+      var visitor = _Visitor(this);
+      registry.addMethodInvocation(this, visitor);
+    }
+  }
+}
+
+class _Visitor extends SimpleAstVisitor<void> {
+  final LintRule rule;
+
+  _Visitor(this.rule);
+
+  @override
+  void visitMethodInvocation(MethodInvocation node) {
+    var arguments = node.argumentList.arguments;
+    for (var sourceString in arguments) {
+      if (sourceString is! SimpleStringLiteral) return;
+
+      var literal = sourceString.literal;
+      if (literal is! StringToken) return;
+
+      if (literal.lexeme.contains(' \n')) {
+        rule.reportLintForToken(literal);
+      }
+    }
+  }
+}