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This is my code:
RSEM/software/RSEM-1.2.25/rsem-prepare-reference --gtf ~/Data/M_musculus/GCF_000001635.27_GRCm39_genomic.gtf --bowtie2 --bowtie2-path RSEM/software/bowtie2-2.2.6 ~/Data/M_musculus/GCF_000001635.26_GRCm38.p6_genomic.fna ~/RNA_Analysis/02_Mapping/mouse_ref
But I'm having this error:
rsem-extract-reference-transcripts /home/luisafmc/RNA_Analysis/02_Mapping/mouse_ref 0 /home/luisafmc/Data/M_musculus/GCF_000001635.27_GRCm39_genomic.gtf 0 /home/luisafmc/Data/M_musculus/GCF_000001635.26_GRCm38.p6_genomic.fna
The GTF file might be corrupted!
Stop at line : NC_000067.7 cmsearch exon 3172239 3172348 . + . gene_id "Gm26206"; transcript_id "XR_004936710.1"; db_xref "GeneID:115487594"; db_xref "RFAM:RF00026"; gbkey "ncRNA"; gene "Gm26206"; inference "COORDINATES: nucleotide motif:Rfam:12.0:RF00026"; inference "COORDINATES: profile:INFERNAL:1.1.1"; product "U6 spliceosomal RNA"; exon_number "1";
Error Message: Cannot separate the identifier from the value for attribute !
"rsem-extract-reference-transcripts /home/luisafmc/RNA_Analysis/02_Mapping/mouse_ref 0 /home/luisafmc/Data/M_musculus/GCF_000001635.27_GRCm39_genomic.gtf 0 /home/luisafmc/Data/M_musculus/GCF_000001635.26_GRCm38.p6_genomic.fna" failed! Plase check if you provide correct parameters/options for the pipeline!
Could you help me?
The text was updated successfully, but these errors were encountered:
Hi,
I'm trying to build an index with the annotation file and fasta file of the most recent from of mouse reference genome. I've donwloaded from NCBI ftg (https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/635/GCF_000001635.27_GRCm39/GCF_000001635.27_GRCm39_genomic.fna.gz and https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/635/GCF_000001635.27_GRCm39/GCF_000001635.27_GRCm39_genomic.gtf.gz)
This is my code:
RSEM/software/RSEM-1.2.25/rsem-prepare-reference --gtf ~/Data/M_musculus/GCF_000001635.27_GRCm39_genomic.gtf --bowtie2 --bowtie2-path RSEM/software/bowtie2-2.2.6 ~/Data/M_musculus/GCF_000001635.26_GRCm38.p6_genomic.fna ~/RNA_Analysis/02_Mapping/mouse_ref
But I'm having this error:
rsem-extract-reference-transcripts /home/luisafmc/RNA_Analysis/02_Mapping/mouse_ref 0 /home/luisafmc/Data/M_musculus/GCF_000001635.27_GRCm39_genomic.gtf 0 /home/luisafmc/Data/M_musculus/GCF_000001635.26_GRCm38.p6_genomic.fna
The GTF file might be corrupted!
Stop at line : NC_000067.7 cmsearch exon 3172239 3172348 . + . gene_id "Gm26206"; transcript_id "XR_004936710.1"; db_xref "GeneID:115487594"; db_xref "RFAM:RF00026"; gbkey "ncRNA"; gene "Gm26206"; inference "COORDINATES: nucleotide motif:Rfam:12.0:RF00026"; inference "COORDINATES: profile:INFERNAL:1.1.1"; product "U6 spliceosomal RNA"; exon_number "1";
Error Message: Cannot separate the identifier from the value for attribute !
"rsem-extract-reference-transcripts /home/luisafmc/RNA_Analysis/02_Mapping/mouse_ref 0 /home/luisafmc/Data/M_musculus/GCF_000001635.27_GRCm39_genomic.gtf 0 /home/luisafmc/Data/M_musculus/GCF_000001635.26_GRCm38.p6_genomic.fna" failed! Plase check if you provide correct parameters/options for the pipeline!
Could you help me?
The text was updated successfully, but these errors were encountered: