From 212be51544f9928d2a4c8283c4f2160780a5e518 Mon Sep 17 00:00:00 2001 From: Shane Giles Date: Thu, 29 Feb 2024 16:14:43 -0700 Subject: [PATCH 01/54] bash run script for gene workflow --- bin/run_uta_build_gene.sh | 34 ++++++++++++++++++++++++++++++++++ 1 file changed, 34 insertions(+) create mode 100755 bin/run_uta_build_gene.sh diff --git a/bin/run_uta_build_gene.sh b/bin/run_uta_build_gene.sh new file mode 100755 index 0000000..b274725 --- /dev/null +++ b/bin/run_uta_build_gene.sh @@ -0,0 +1,34 @@ +#!/bin/bash + +export uta_v=uta_20210129b + +## get the file +rsync --no-motd -HRavP ftp.ncbi.nlm.nih.gov::gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz . + +## extract meta data +sbin/ncbi-parse-geneinfo DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz | gzip -c > genes.geneinfo.gz + +### pull a uta database +docker pull biocommons/uta:$uta_v + +### stand up container for UTA database +docker run \ + -d \ + -e POSTGRES_PASSWORD=temp \ + -v /tmp:/tmp \ + -v uta_vol:/var/lib/postgresql/data \ +# -p 5432:5432 \ + --name $uta_v \ + --network=host \ + biocommons/uta:$uta_v + +docker ps -a + +## update the uta database +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo genes.geneinfo.gz + +## psql_dump + +## stop local postgres container +docker stop uta_20210129b +docker rm uta_20210129b From fac3e92e0e0ed2d004efc71609f870b343e0e595 Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Thu, 29 Feb 2024 16:20:22 -0800 Subject: [PATCH 02/54] fix: wip --- etc/uta_dev@localhost.conf | 4 ++-- src/uta/loading.py | 13 +++++++------ src/uta/models.py | 2 +- 3 files changed, 10 insertions(+), 9 deletions(-) diff --git a/etc/uta_dev@localhost.conf b/etc/uta_dev@localhost.conf index 2d1e508..2c3635b 100644 --- a/etc/uta_dev@localhost.conf +++ b/etc/uta_dev@localhost.conf @@ -1,4 +1,4 @@ [uta] -hostport = localhost +hostport = 127.0.0.1 cluster = %(user)s@%(hostport)s -database = uta_dev +database = uta diff --git a/src/uta/loading.py b/src/uta/loading.py index 2bc9b5e..1d272d3 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -13,10 +13,11 @@ from bioutils.digests import seq_md5 from bioutils.sequences import reverse_complement from sqlalchemy.exc import IntegrityError +from sqlalchemy import text from sqlalchemy.orm.exc import NoResultFound import psycopg2.extras import six -import uta_align.align.algorithms as utaaa +# import uta_align.align.algorithms as utaaa from uta.lru_cache import lru_cache @@ -199,8 +200,8 @@ def drop_schema(session, opts, cf): def grant_permissions(session, opts, cf): schema = usam.schema_name - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) session.execute("set search_path = " + usam.schema_name) cmds = [ @@ -282,9 +283,9 @@ def load_exonset(session, opts, cf): def load_geneinfo(session, opts, cf): - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) gir = ufgi.GeneInfoReader(gzip.open(opts["FILE"], 'rt')) logger.info("opened " + opts["FILE"]) diff --git a/src/uta/models.py b/src/uta/models.py index 2305666..6cd64ed 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -13,7 +13,7 @@ # schema name support # also see etc/uta.conf -schema_version = "1.1" +schema_version = "20210129b" use_schema = True if use_schema: schema_name = "uta_" + schema_version.replace(".","_") From 5155c01018f19a80f4711ff0e814cd17ae0d1610 Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Thu, 29 Feb 2024 16:34:58 -0800 Subject: [PATCH 03/54] fix: wip --- bin/run_uta_build_gene.sh | 18 +++++------------- etc/uta_dev@localhost.conf | 2 +- 2 files changed, 6 insertions(+), 14 deletions(-) diff --git a/bin/run_uta_build_gene.sh b/bin/run_uta_build_gene.sh index b274725..73287eb 100755 --- a/bin/run_uta_build_gene.sh +++ b/bin/run_uta_build_gene.sh @@ -12,23 +12,15 @@ sbin/ncbi-parse-geneinfo DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz | gzi docker pull biocommons/uta:$uta_v ### stand up container for UTA database -docker run \ - -d \ - -e POSTGRES_PASSWORD=temp \ - -v /tmp:/tmp \ - -v uta_vol:/var/lib/postgresql/data \ -# -p 5432:5432 \ - --name $uta_v \ - --network=host \ - biocommons/uta:$uta_v - -docker ps -a +docker run -d -v /tmp:/tmp -v uta_vol:/var/lib/postgresql/data --name $uta_v --network=host biocommons/uta:$uta_v +sleep 5 +docker ps ## update the uta database -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo genes.geneinfo.gz +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo genes.sample.gz ## psql_dump - +pg_dump -U uta_admin -h localhost -d uta -t uta_20210129b.gene | gzip -c > uta.pgd.gz ## stop local postgres container docker stop uta_20210129b docker rm uta_20210129b diff --git a/etc/uta_dev@localhost.conf b/etc/uta_dev@localhost.conf index 2c3635b..bfb5c66 100644 --- a/etc/uta_dev@localhost.conf +++ b/etc/uta_dev@localhost.conf @@ -1,4 +1,4 @@ [uta] -hostport = 127.0.0.1 +hostport = localhost cluster = %(user)s@%(hostport)s database = uta From ea521e6c86d432340c85a33d9bb11ff754b27fc2 Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Tue, 5 Mar 2024 16:04:48 -0800 Subject: [PATCH 04/54] feat(IPVC-2231): add script to parse gff --- sbin/ncbi_parse_genomic_gff.py | 98 ++++++++++++++++++++++++++++++++++ 1 file changed, 98 insertions(+) create mode 100644 sbin/ncbi_parse_genomic_gff.py diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py new file mode 100644 index 0000000..4e91ba4 --- /dev/null +++ b/sbin/ncbi_parse_genomic_gff.py @@ -0,0 +1,98 @@ +import argparse +import os +from typing import List, Optional +import gzip +from collections import defaultdict +from dataclasses import dataclass + +import logging + +from uta.formats.exonset import ExonSet, ExonSetWriter + +logging.basicConfig(level=logging.INFO) +logger = logging.getLogger(__name__) + + +@dataclass +class GFFRecord: + seqid: str + start: int + end: int + strand: str + exon_number: int + parent_id: str + transcript_id: str + + +def _sort_exons(exons: List[GFFRecord]) -> List[GFFRecord]: + return sorted(exons, key=lambda e: e.exon_number) + + +def parse_gff_record(line: str) -> Optional[GFFRecord]: + """Parses a single line from a GFF file and returns a GFFRecord if record is an exon aligned to an NC_ chromosome and has a transcript id starting with NM_ or NR_.""" + # NC_000001.10 BestRefSeq exon 11874 12227 . + . ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 + # NC_000001.10 BestRefSeq exon 12613 12721 . + . ID=exon-NR_046018.2-2;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 + # NC_000001.10 BestRefSeq exon 13221 14409 . + . ID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 + + fields = line.strip().split('\t') + seqid, source, feature, start, end, score, strand, phase, attributes_str = fields + if not feature == 'exon': + return + if not seqid.startswith('NC_'): + return + + attributes = {} + for attr_str in attributes_str.split(';'): + if '=' in attr_str: + key, value = attr_str.split('=') + attributes[key.lower()] = value + + parent_id = attributes.get('parent') + transcript_id = attributes.get('transcript_id') + if not transcript_id or (not transcript_id.startswith('NM_') and not transcript_id.startswith('NR_')): + return + exon_number = int(attributes.get('id').split('-')[-1]) + return GFFRecord(seqid=seqid, start=int(start), end=int(end), strand=strand, exon_number=exon_number, parent_id=parent_id, transcript_id=transcript_id) + + +def parse_gff_file(file_path: str) -> dict[str, List[GFFRecord]]: + tx_data = defaultdict(list) + with gzip.open(file_path, 'rt') as f: + for line in f: + if line.startswith('#'): + continue + try: + record = parse_gff_record(line) + except (AttributeError, ValueError, KeyError): + raise Exception(f'failed at line :{line}') + if record: + tx_data[record.parent_id].append(record) + return {k: _sort_exons(v) for k, v in tx_data.items()} + + +def main(): + parser = argparse.ArgumentParser(description="Parse GFF file.") + parser.add_argument("gff_file", type=argparse.FileType('r'), help="Path to the gzipped GFF file") + parser.add_argument("--prefix", "-p", type=str, default="ncbi-gff", help="Output filename (default: ncbi-gff)") + args = parser.parse_args() + + gff_infile = args.gff_file + exonset_outfile = args.prefix + "exonset.gz" + esw = ExonSetWriter(gzip.open(exonset_outfile + ".tmp", "wt")) + + transcript_alignments = parse_gff_file(gff_infile) + logger.info( + f"read {len(transcript_alignments)} transcript alignments from {gff_infile}") + + for transcript_exons in transcript_alignments.values(): + exons_se = ';'.join(','.join(map(str, tpl)) for tpl in [(ex.start, ex.end) for ex in transcript_exons]) + e = transcript_exons[0] + es = ExonSet(tx_ac=e.transcript_id, alt_ac=e.seqid, method='splign', strand=e.strand, exons_se_i=exons_se) + esw.write(es) + os.rename(exonset_outfile + ".tmp", exonset_outfile) + + +if __name__ == "__main__": + main() + + From bd646bb4732826835b8e86bc06ef4947e120267b Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Wed, 6 Mar 2024 10:43:04 -0800 Subject: [PATCH 05/54] test(IPVC-2231): add test for parsing genomic gff --- tests/test_ncbi_parse_genomic_gff.py | 47 ++++++++++++++++++++++++++++ 1 file changed, 47 insertions(+) create mode 100644 tests/test_ncbi_parse_genomic_gff.py diff --git a/tests/test_ncbi_parse_genomic_gff.py b/tests/test_ncbi_parse_genomic_gff.py new file mode 100644 index 0000000..8cfab13 --- /dev/null +++ b/tests/test_ncbi_parse_genomic_gff.py @@ -0,0 +1,47 @@ +import unittest +from tempfile import NamedTemporaryFile +import os +import gzip + +from sbin.ncbi_parse_genomic_gff import GFFRecord, parse_gff_file, parse_gff_record + + +class TestGFFParsing(unittest.TestCase): + def setUp(self): + with NamedTemporaryFile(suffix='.gz', delete=False) as temp_gff: + with gzip.open(temp_gff.name, 'wt') as f: + f.write( + "NC_000001.10\tBestRefSeq\texon\t11874\t12227\t.\t+\t.\tID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n") + f.write( + "NC_000001.10\tBestRefSeq\texon\t12613\t12721\t.\t+\t.\tID=exon-NR_046018.2-2;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n") + f.write( + "NC_000001.10\tBestRefSeq\texon\t13221\t14409\t.\t+\t.\tID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n") + temp_gff.seek(0) + self.temp_gff = temp_gff + + + def tearDown(self): + os.remove(self.temp_gff.name) + + def test_parse_gff_record(self): + # Test parsing a single GFF record + line = "NC_000001.10\tBestRefSeq\texon\t11874\t12227\t.\t+\t.\tID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n" + expected_record = GFFRecord(seqid="NC_000001.10", start=11874, end=12227, strand="+", exon_number=1, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2") + parsed_record = parse_gff_record(line) + self.assertEqual(parsed_record, expected_record) + + def test_parse_gff_file(self): + # Test parsing the entire GFF file + expected_result = { + "rna-NR_046018.2": [ + GFFRecord(seqid="NC_000001.10", start=11874, end=12227, strand="+", exon_number=1, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2"), + GFFRecord(seqid="NC_000001.10", start=12613, end=12721, strand="+", exon_number=2, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2"), + GFFRecord(seqid="NC_000001.10", start=13221, end=14409, strand="+", exon_number=3, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2") + ] + } + parsed_result = parse_gff_file(self.temp_gff.name) + self.assertEqual(parsed_result, expected_result) + + +if __name__ == "__main__": + unittest.main() \ No newline at end of file From 19313db51ca8cd966d2875d85302de802c866ec4 Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Wed, 6 Mar 2024 15:27:55 -0800 Subject: [PATCH 06/54] test(IPVC-2231): add test for parsing genomic gff and file --- sbin/ncbi_parse_genomic_gff.py | 52 ++++++--- tests/data/expected_genomic_100.exonset.gz | Bin 0 -> 393 bytes tests/data/genomic_100.gff.gz | Bin 0 -> 3704 bytes tests/test_ncbi_parse_genomic_gff.py | 121 +++++++++++++++++++-- 4 files changed, 149 insertions(+), 24 deletions(-) create mode 100644 tests/data/expected_genomic_100.exonset.gz create mode 100644 tests/data/genomic_100.gff.gz diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 4e91ba4..b9bac9b 100644 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -35,10 +35,12 @@ def parse_gff_record(line: str) -> Optional[GFFRecord]: # NC_000001.10 BestRefSeq exon 13221 14409 . + . ID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 fields = line.strip().split('\t') + if len(fields) != 9: + raise ValueError(f'Expected 9 tab-separated fields, got {len(fields)}') + seqid, source, feature, start, end, score, strand, phase, attributes_str = fields - if not feature == 'exon': - return - if not seqid.startswith('NC_'): + + if feature != 'exon' or not seqid.startswith('NC_'): return attributes = {} @@ -49,12 +51,24 @@ def parse_gff_record(line: str) -> Optional[GFFRecord]: parent_id = attributes.get('parent') transcript_id = attributes.get('transcript_id') - if not transcript_id or (not transcript_id.startswith('NM_') and not transcript_id.startswith('NR_')): + if not transcript_id or (not transcript_id.startswith('NM_') and not transcript_id.startswith('NR_')) or not parent_id: return - exon_number = int(attributes.get('id').split('-')[-1]) + try: + exon_number = _get_exon_number_from_id(alignment_id=attributes.get('id')) + except (ValueError, IndexError): + raise ValueError(f'Failed to parse exon number from {attributes.get("id")}') + return GFFRecord(seqid=seqid, start=int(start), end=int(end), strand=strand, exon_number=exon_number, parent_id=parent_id, transcript_id=transcript_id) +def _get_exon_number_from_id(alignment_id: str) -> int: + """ + Pulls the exon number from the alignment id. Expects the id to be in the format + exon-- + """ + return int(alignment_id.split('-')[-1]) + + def parse_gff_file(file_path: str) -> dict[str, List[GFFRecord]]: tx_data = defaultdict(list) with gzip.open(file_path, 'rt') as f: @@ -63,36 +77,42 @@ def parse_gff_file(file_path: str) -> dict[str, List[GFFRecord]]: continue try: record = parse_gff_record(line) - except (AttributeError, ValueError, KeyError): - raise Exception(f'failed at line :{line}') + except ValueError as e: + raise Exception(f'Failed at line :{line} with error: {e}') if record: tx_data[record.parent_id].append(record) return {k: _sort_exons(v) for k, v in tx_data.items()} -def main(): +def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: + """Convert exon ranges to 0-based half-open format""" + formatted_exons = [] + for ex in transcript_exons: + formatted_exons.append(','.join(map(str, (ex.start - 1, ex.end)))) + return ';'.join(formatted_exons) + + +if __name__ == "__main__": parser = argparse.ArgumentParser(description="Parse GFF file.") - parser.add_argument("gff_file", type=argparse.FileType('r'), help="Path to the gzipped GFF file") + parser.add_argument("gff_file", type=argparse.FileType('rb'), help="Path to the gzipped GFF file") parser.add_argument("--prefix", "-p", type=str, default="ncbi-gff", help="Output filename (default: ncbi-gff)") args = parser.parse_args() gff_infile = args.gff_file - exonset_outfile = args.prefix + "exonset.gz" - esw = ExonSetWriter(gzip.open(exonset_outfile + ".tmp", "wt")) + exonset_outfile = args.prefix + ".exonset.gz" + output_file = gzip.open(exonset_outfile + ".tmp", "wt") + esw = ExonSetWriter(output_file) transcript_alignments = parse_gff_file(gff_infile) logger.info( f"read {len(transcript_alignments)} transcript alignments from {gff_infile}") for transcript_exons in transcript_alignments.values(): - exons_se = ';'.join(','.join(map(str, tpl)) for tpl in [(ex.start, ex.end) for ex in transcript_exons]) + exons_se = get_zero_based_exon_ranges(transcript_exons) e = transcript_exons[0] es = ExonSet(tx_ac=e.transcript_id, alt_ac=e.seqid, method='splign', strand=e.strand, exons_se_i=exons_se) esw.write(es) + output_file.close() os.rename(exonset_outfile + ".tmp", exonset_outfile) -if __name__ == "__main__": - main() - - diff --git a/tests/data/expected_genomic_100.exonset.gz b/tests/data/expected_genomic_100.exonset.gz new file mode 100644 index 0000000000000000000000000000000000000000..82d26801b660453e8e460dbb4ce2ce7d1092ad44 GIT binary patch literal 393 zcmV;40e1c$iwFQh?&xIz1AS7vZreZ*tf^n|McDTPEK^5Cet-~SAu-^{Mx?@@pCMJG z3C-ee_P94YJA3z!<@{qhzuwpF_vQZU?RmQ0|D3PS)8*ssdRuOn<>hetzK{iR9c4Ow zU1BXzQU{Aj&Md(KoWe7!2n{x)-dM!k=*a(N`7%i>vau%3VyQXA zs=-ncIk+@p=4>`#3Y=$<56(0J7Dd(6sF|Z2`AP1;K`}TofIS=e-UgM{(ZHn0Hw}i$ zl1(L3G(CLkWc*4n%#zt)6fIjK#CMWDIp%u?6GYQT1^8 zhMP=;AGSM?aN`GWpc$J~u92Frk!c|+FaFn{2=6wi>qKz{qwd_MCf=UA&U%=14Q!Ax nd#D;Ag4K#h(tC~MF(nSc`}l+_yAAnxg?{)8SJKxTL<0Z-{K&>r literal 0 HcmV?d00001 diff --git a/tests/data/genomic_100.gff.gz b/tests/data/genomic_100.gff.gz new file mode 100644 index 0000000000000000000000000000000000000000..34f7af100cad1fe6623d377210876b05c6e6fa96 GIT binary patch literal 3704 zcmV-;4u|m{iwFR@v*%?11MOV>Puw^f{+azNa;jFk({=`bI{~2+9STKP=p3+jE3H3o%pqr6S;=F_>ChKo3wrQVMVRqn<+tnzvn(Ig7f$iu1D=QK)Gu4VY8KfaCs8s8iQ0*UYRI(9CikC)MJJ9+gxc&Dmyr@!+9{69T9 z(DU!%e!t4He{}sNi9Q}IRi%4M_WpS|jSles_$~oH(s}mF?e(wG{lR52-p@Pap#Hi) zoli!~`$cq+eTv84(fMJsfp4O8Su}EyMys1S@zsQS`7I{FFY_f^|Y zAno*M4q0UB~kb;W7zlO8RwlGpY;k=Rd;NRn!W+EV@{_fhV)D zcYZNKOd#y{$^I}*qS3vCj(E@dZxxK#B8E4vpnhjY(Bf69vmN@(ct*CD$a*n>rxjDW&ab#8S3MP zF70AR9}h)YH)FbR*8nLhDaXeqY<3TfqO$H;0xZBRF}SE^Ng=}4pCyn7Q}T=$V3PXq z^aI?%fTc8F5ChmJ!iOgnqJsfTQ@$hyWPmv^fJE0AU@w{haO2@A8R=kvzi0*s1?Ndp zm<#O;h?mTO@HyxKi8$|IK)z@O1d{NSqKg6lB{RTf0D6E2Om;93ykrJg0KO3Bh<51# zj9xSa%0b4=CqWklFVri{LnW{S0X%^TBUCy&y#PHK^z5Y-!i11qN5v7M!Ru*!@dm=X zy|Yt@A_=J1JRqnA=~2`)9w&1E?-k$7h^a2s2%4adg0Lz^x=X{}G){VX_V)NNiKk(5 zKhgsv7xPmSpAD%BKh?t{g|e=4OXxMMv%@PuCIt>Q#y%T&V9rPUMLy!` zVzZ8=R@_$!Zem+M$iF_avd`3_mm0HeeP+e859u}ke}Ijz?KKB9qt{4FzxC`@z2}22 zvpN*17@Q(-K8)=R5R54z4hV><#^|l3oCK`VS|q>(S5~JGDJl_oI-oDP?-4T^hJ(fu(_qblVdtgm#a3Cl{47R*0}y z=*k8^Eg!!t{9Kt)#K04WC-<9Up{{aE=*DD6+z-&k*qf8xz+6stmAtN39jHn@mP@s? 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ID=exon-NR_046018.2-2;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 # NC_000001.10 BestRefSeq exon 13221 14409 . + . ID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 - fields = line.strip().split('\t') + fields = line.strip().split("\t") if len(fields) != 9: - raise ValueError(f'Expected 9 tab-separated fields, got {len(fields)}') + raise ValueError(f"Expected 9 tab-separated fields, got {len(fields)}") seqid, source, feature, start, end, score, strand, phase, attributes_str = fields - if feature != 'exon' or not seqid.startswith('NC_'): + if feature != "exon" or not seqid.startswith("NC_"): return attributes = {} - for attr_str in attributes_str.split(';'): - if '=' in attr_str: - key, value = attr_str.split('=') + for attr_str in attributes_str.split(";"): + if "=" in attr_str: + key, value = attr_str.split("=") attributes[key.lower()] = value - parent_id = attributes.get('parent') - transcript_id = attributes.get('transcript_id') - if not transcript_id or (not transcript_id.startswith('NM_') and not transcript_id.startswith('NR_')) or not parent_id: + parent_id = attributes.get("parent") + transcript_id = attributes.get("transcript_id") + if ( + not transcript_id + or (not transcript_id.startswith("NM_") and not transcript_id.startswith("NR_")) + or not parent_id + ): return try: - exon_number = _get_exon_number_from_id(alignment_id=attributes.get('id')) + exon_number = _get_exon_number_from_id(alignment_id=attributes.get("id")) except (ValueError, IndexError): raise ValueError(f'Failed to parse exon number from {attributes.get("id")}') - return GFFRecord(seqid=seqid, start=int(start), end=int(end), strand=strand, exon_number=exon_number, parent_id=parent_id, transcript_id=transcript_id) + return GFFRecord( + seqid=seqid, + start=int(start), + end=int(end), + strand=strand, + exon_number=exon_number, + parent_id=parent_id, + transcript_id=transcript_id, + ) def _get_exon_number_from_id(alignment_id: str) -> int: @@ -66,19 +78,19 @@ def _get_exon_number_from_id(alignment_id: str) -> int: Pulls the exon number from the alignment id. Expects the id to be in the format exon-- """ - return int(alignment_id.split('-')[-1]) + return int(alignment_id.split("-")[-1]) def parse_gff_file(file_path: str) -> dict[str, List[GFFRecord]]: tx_data = defaultdict(list) - with gzip.open(file_path, 'rt') as f: + with gzip.open(file_path, "rt") as f: for line in f: - if line.startswith('#'): + if line.startswith("#"): continue try: record = parse_gff_record(line) except ValueError as e: - raise Exception(f'Failed at line :{line} with error: {e}') + raise Exception(f"Failed at line :{line} with error: {e}") if record: tx_data[record.parent_id].append(record) return {k: _sort_exons(v) for k, v in tx_data.items()} @@ -88,14 +100,22 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: """Convert exon ranges to 0-based half-open format""" formatted_exons = [] for ex in transcript_exons: - formatted_exons.append(','.join(map(str, (ex.start - 1, ex.end)))) - return ';'.join(formatted_exons) + formatted_exons.append(",".join(map(str, (ex.start - 1, ex.end)))) + return ";".join(formatted_exons) if __name__ == "__main__": parser = argparse.ArgumentParser(description="Parse GFF file.") - parser.add_argument("gff_file", type=argparse.FileType('rb'), help="Path to the gzipped GFF file") - parser.add_argument("--prefix", "-p", type=str, default="ncbi-gff", help="Output filename (default: ncbi-gff)") + parser.add_argument( + "gff_file", type=argparse.FileType("rb"), help="Path to the gzipped GFF file" + ) + parser.add_argument( + "--prefix", + "-p", + type=str, + default="ncbi-gff", + help="Output filename (default: ncbi-gff)", + ) args = parser.parse_args() gff_infile = args.gff_file @@ -105,14 +125,19 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: transcript_alignments = parse_gff_file(gff_infile) logger.info( - f"read {len(transcript_alignments)} transcript alignments from {gff_infile}") + f"read {len(transcript_alignments)} transcript alignments from {gff_infile}" + ) for transcript_exons in transcript_alignments.values(): exons_se = get_zero_based_exon_ranges(transcript_exons) e = transcript_exons[0] - es = ExonSet(tx_ac=e.transcript_id, alt_ac=e.seqid, method='splign', strand=e.strand, exons_se_i=exons_se) + es = ExonSet( + tx_ac=e.transcript_id, + alt_ac=e.seqid, + method="splign", + strand=e.strand, + exons_se_i=exons_se, + ) esw.write(es) output_file.close() os.rename(exonset_outfile + ".tmp", exonset_outfile) - - diff --git a/tests/test_ncbi_parse_genomic_gff.py b/tests/test_ncbi_parse_genomic_gff.py index 7fe3eb6..7f66b0d 100644 --- a/tests/test_ncbi_parse_genomic_gff.py +++ b/tests/test_ncbi_parse_genomic_gff.py @@ -4,7 +4,12 @@ import os import gzip -from sbin.ncbi_parse_genomic_gff import GFFRecord, parse_gff_file, parse_gff_record, get_zero_based_exon_ranges +from sbin.ncbi_parse_genomic_gff import ( + GFFRecord, + parse_gff_file, + parse_gff_record, + get_zero_based_exon_ranges, +) CURRENT_DIR = os.path.dirname(os.path.abspath(__file__)) BASE_DIR = os.path.dirname(CURRENT_DIR) @@ -12,37 +17,64 @@ def sample_line(**params): defaults = { - 'seqid': 'NC_000001.10', - 'source': 'BestRefSeq', - 'feature': 'exon', - 'start': 11874, - 'stop': 12227, - 'score': '.', - 'strand': '+', - 'phase': '.', - 'attributes_str': 'ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=NR_046018.2' + "seqid": "NC_000001.10", + "source": "BestRefSeq", + "feature": "exon", + "start": 11874, + "stop": 12227, + "score": ".", + "strand": "+", + "phase": ".", + "attributes_str": "ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=NR_046018.2", } defaults.update(params) - return '\t'.join(map(str, defaults.values())) + '\n' + return "\t".join(map(str, defaults.values())) + "\n" class TestGFFParsing(unittest.TestCase): def setUp(self): - with NamedTemporaryFile(suffix='.gz', delete=False) as temp_gff: - with gzip.open(temp_gff.name, 'wt') as f: + with NamedTemporaryFile(suffix=".gz", delete=False) as temp_gff: + with gzip.open(temp_gff.name, "wt") as f: f.write( - "NC_000001.10\tBestRefSeq\texon\t11874\t12227\t.\t+\t.\tID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n") + "NC_000001.10\tBestRefSeq\texon\t11874\t12227\t.\t+\t.\tID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n" + ) f.write( - "NC_000001.10\tBestRefSeq\texon\t12613\t12721\t.\t+\t.\tID=exon-NR_046018.2-2;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n") + "NC_000001.10\tBestRefSeq\texon\t12613\t12721\t.\t+\t.\tID=exon-NR_046018.2-2;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n" + ) f.write( - "NC_000001.10\tBestRefSeq\texon\t13221\t14409\t.\t+\t.\tID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n") + "NC_000001.10\tBestRefSeq\texon\t13221\t14409\t.\t+\t.\tID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n" + ) temp_gff.seek(0) self.temp_gff = temp_gff self.gff_records = [ - GFFRecord(seqid="NC_000001.10", start=11874, end=12227, strand="+", exon_number=1, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2"), - GFFRecord(seqid="NC_000001.10", start=12613, end=12721, strand="+", exon_number=2, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2"), - GFFRecord(seqid="NC_000001.10", start=13221, end=14409, strand="+", exon_number=3, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2") - ] + GFFRecord( + seqid="NC_000001.10", + start=11874, + end=12227, + strand="+", + exon_number=1, + parent_id="rna-NR_046018.2", + transcript_id="NR_046018.2", + ), + GFFRecord( + seqid="NC_000001.10", + start=12613, + end=12721, + strand="+", + exon_number=2, + parent_id="rna-NR_046018.2", + transcript_id="NR_046018.2", + ), + GFFRecord( + seqid="NC_000001.10", + start=13221, + end=14409, + strand="+", + exon_number=3, + parent_id="rna-NR_046018.2", + transcript_id="NR_046018.2", + ), + ] def tearDown(self): os.remove(self.temp_gff.name) @@ -50,41 +82,55 @@ def tearDown(self): def test_parse_gff_record(self): # Test parsing a single GFF record line = sample_line() - expected_record = GFFRecord(seqid="NC_000001.10", start=11874, end=12227, strand="+", exon_number=1, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2") + expected_record = GFFRecord( + seqid="NC_000001.10", + start=11874, + end=12227, + strand="+", + exon_number=1, + parent_id="rna-NR_046018.2", + transcript_id="NR_046018.2", + ) parsed_record = parse_gff_record(line) self.assertEqual(parsed_record, expected_record) def test_parse_gff_record_skips_non_exon_records(self): # We exclude non-exon records - line = sample_line(feature='pseudogene') + line = sample_line(feature="pseudogene") expected_record = None parsed_record = parse_gff_record(line) self.assertEqual(parsed_record, expected_record) def test_parse_gff_record_skips_non_NC_target(self): # We exclude alignments to non-NC chromosomes - line = sample_line(seqid='NT_123') + line = sample_line(seqid="NT_123") expected_record = None parsed_record = parse_gff_record(line) self.assertEqual(parsed_record, expected_record) def test_parse_gff_record_skips_missing_transcript_id(self): # We exclude alignments missing transcript_id - line = sample_line(attributes_str='ID=exon-NR_046018.2-1;transcript_id=NR_046018.2') # parent missing from attributes + line = sample_line( + attributes_str="ID=exon-NR_046018.2-1;transcript_id=NR_046018.2" + ) # parent missing from attributes expected_record = None parsed_record = parse_gff_record(line) self.assertEqual(parsed_record, expected_record) def test_parse_gff_record_skips_missing_parent_field(self): # We exclude alignments missing a parent field - line = sample_line(attributes_str='ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2') # transcript_id missing from attributes + line = sample_line( + attributes_str="ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2" + ) # transcript_id missing from attributes expected_record = None parsed_record = parse_gff_record(line) self.assertEqual(parsed_record, expected_record) def test_parse_gff_record_skips_non_NM_NR_transcripts(self): # We only care about transcripts that start with NM_ or NR_ - line = sample_line(attributes_str='ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=somethingelse') # transcript_id missing from attributes + line = sample_line( + attributes_str="ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=somethingelse" + ) # transcript_id missing from attributes expected_record = None parsed_record = parse_gff_record(line) self.assertEqual(parsed_record, expected_record) @@ -95,7 +141,9 @@ def test_parse_gff_record_unexpected_number_of_fields(self): with self.assertRaises(ValueError) as context: parse_gff_record(line) - self.assertEqual(str(context.exception), "Expected 9 tab-separated fields, got 2") + self.assertEqual( + str(context.exception), "Expected 9 tab-separated fields, got 2" + ) def test_parse_gff_record_raises_non_int_start_stop(self): # Raise an exception if either start or stop is not an integer @@ -106,17 +154,19 @@ def test_parse_gff_record_raises_non_int_start_stop(self): def test_parse_gff_record_raises_unparseable_id(self): # raise an exception if we cannot parse the exon number from the ID - line = sample_line(attributes_str='ID=unexpected_id;Parent=rna-NR_046018.2;transcript_id=NR_046018') + line = sample_line( + attributes_str="ID=unexpected_id;Parent=rna-NR_046018.2;transcript_id=NR_046018" + ) with self.assertRaises(ValueError) as context: parse_gff_record(line) - self.assertEqual(str(context.exception), "Failed to parse exon number from unexpected_id") + self.assertEqual( + str(context.exception), "Failed to parse exon number from unexpected_id" + ) def test_parse_gff_file(self): # Test parsing the entire GFF file - expected_result = { - "rna-NR_046018.2": self.gff_records - } + expected_result = {"rna-NR_046018.2": self.gff_records} parsed_result = parse_gff_file(self.temp_gff.name) self.assertEqual(parsed_result, expected_result) @@ -127,26 +177,34 @@ def test_get_zero_based_exon_ranges(self): def test_script_output(self): # Run the script from the command line - file_prefix = 'genomic_100' - input_gff_file = os.path.join(CURRENT_DIR, 'data', f'{file_prefix}.gff.gz') + file_prefix = "genomic_100" + input_gff_file = os.path.join(CURRENT_DIR, "data", f"{file_prefix}.gff.gz") - script_path = os.path.join(BASE_DIR, 'sbin', 'ncbi_parse_genomic_gff.py') - expected_output_file = os.path.join(CURRENT_DIR, 'data', f'expected_{file_prefix}.exonset.gz') - output_file = os.path.join(os.getcwd(), f'{file_prefix}.exonset.gz') + script_path = os.path.join(BASE_DIR, "sbin", "ncbi_parse_genomic_gff.py") + expected_output_file = os.path.join( + CURRENT_DIR, "data", f"expected_{file_prefix}.exonset.gz" + ) + output_file = os.path.join(os.getcwd(), f"{file_prefix}.exonset.gz") - command = ['python', script_path, input_gff_file, '--prefix', file_prefix] + command = ["python", script_path, input_gff_file, "--prefix", file_prefix] subprocess.run(command, check=True) # Compare the generated output file with the expected file self.assertTrue(os.path.isfile(output_file), "Output file not generated.") - with gzip.open(expected_output_file, 'rb') as expected_file, gzip.open(output_file, 'rb') as actual_file: + with gzip.open(expected_output_file, "rb") as expected_file, gzip.open( + output_file, "rb" + ) as actual_file: expected_content = expected_file.read() actual_content = actual_file.read() - self.assertEqual(expected_content, actual_content, "Output file content doesn't match expected.") + self.assertEqual( + expected_content, + actual_content, + "Output file content doesn't match expected.", + ) # Clean up temporary files if needed os.remove(output_file) if __name__ == "__main__": - unittest.main() \ No newline at end of file + unittest.main() From bec0768da3b8a542c2d2afe24fc05292f25b1024 Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Wed, 6 Mar 2024 16:27:19 -0800 Subject: [PATCH 08/54] feat(IPVC-2231): adjustments, add shell script --- bin/run_uta_parse_gff.sh | 29 +++++++++++++++++++++ sbin/ncbi_parse_genomic_gff.py | 27 +++++++++++++++++-- src/uta/loading.py | 6 ++--- tests/data/expected_genomic_100.exonset.gz | Bin 393 -> 392 bytes tests/test_ncbi_parse_genomic_gff.py | 4 +-- 5 files changed, 59 insertions(+), 7 deletions(-) create mode 100755 bin/run_uta_parse_gff.sh diff --git a/bin/run_uta_parse_gff.sh b/bin/run_uta_parse_gff.sh new file mode 100755 index 0000000..c42cc5e --- /dev/null +++ b/bin/run_uta_parse_gff.sh @@ -0,0 +1,29 @@ +#!/bin/bash + +export uta_v=uta_20210129b + +## get the file +rsync --no-motd -HRavP --no-relative ftp.ncbi.nlm.nih.gov::genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.25_GRCh37.p13/GCF_000001405.25_GRCh37.p13_genomic.gff.gz . + + +## extract exon alignments +python sbin/ncbi_parse_genomic_gff.py GCF_000001405.25_GRCh37.p13_genomic.gff.gz + + +### pull a uta database +docker pull biocommons/uta:$uta_v + +### stand up container for UTA database +docker run -d -v /tmp:/tmp -v uta_vol:/var/lib/postgresql/data --name $uta_v --network=host biocommons/uta:$uta_v +sleep 5 +docker ps + +## update the uta database +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset ncbi-gff.exonset.gz + + +## psql_dump +pg_dump -U uta_admin -h localhost -d uta -t uta_20210129b.gene | gzip -c > uta.pgd.gz +## stop local postgres container +docker stop uta_20210129b +docker rm uta_20210129b diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 6feb572..9ed3806 100644 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -1,3 +1,26 @@ +"""Write exonsets from NCBI GFF alignments, as obtained from +ftp://ftp.ncbi.nlm.nih.gov/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions +This service appeared in April 2015 and is due to update weekly. + +See uta.formats for a description of those file formats. + +In a nutshell, this means that you'll get data like this: + +ncbi-gff.exonsets.gz: +tx_ac alt_ac method strand exons_se_i +NM_130786.3 NC_000019.9 splign -1 58864769,58864865;588646... +NM_130786.3 NC_018930.2 splign -1 58858699,58858795;588585... +NM_130786.3 AC_000151.1 splign -1 55173924,55174020;551738... +NM_138933.2 NC_000010.10 splign -1 52645340,52645435;52... + +UTA requires that the exon structure of a transcript accession as +defined on its own sequence is unique. Although this is mostly true, +there are instances where NCBI reports different exon structures for a +single transcript. For example, NM_001300954.1 aligns with 11 exons on +NC_000011.9 and 5 exons on NW_003871081.1, and the differences are NOT +due merely to concatenation of adjacent spans. +""" + import argparse import os from typing import List, Optional @@ -125,7 +148,7 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: transcript_alignments = parse_gff_file(gff_infile) logger.info( - f"read {len(transcript_alignments)} transcript alignments from {gff_infile}" + f"read {len(transcript_alignments)} transcript alignments from {gff_infile.name}" ) for transcript_exons in transcript_alignments.values(): @@ -135,7 +158,7 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: tx_ac=e.transcript_id, alt_ac=e.seqid, method="splign", - strand=e.strand, + strand=-1 if e.strand == "-" else 1, exons_se_i=exons_se, ) esw.write(es) diff --git a/src/uta/loading.py b/src/uta/loading.py index 1d272d3..f0dc6fc 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -245,9 +245,9 @@ def load_exonset(session, opts, cf): update_period = 25 - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) n_rows = len(gzip.open(opts["FILE"], 'rt').readlines()) - 1 esr = ufes.ExonSetReader(gzip.open(opts["FILE"], 'rt')) diff --git a/tests/data/expected_genomic_100.exonset.gz b/tests/data/expected_genomic_100.exonset.gz index 82d26801b660453e8e460dbb4ce2ce7d1092ad44..6e9dc3a722c382e530637bd98a98c7413a67ad40 100644 GIT binary patch literal 392 zcmV;30eAi%iwFQr2X8E%G6eo9}t9CNDMf#5vlO!dngrY za=wC`kkeAyj-9La*X_Ocwl zA8CHPXIifBuP?tY3op!-I#@(3gLkTxMJc#;=Ts`3dD2rEh$r$Q3u?6GYaqaH# zgihetzK{iR9c4Ow zU1BXzQU{Aj&Md(KoWe7!2n{x)-dM!k=*a(N`7%i>vau%3VyQXA zs=-ncIk+@p=4>`#3Y=$<56(0J7Dd(6sF|Z2`AP1;K`}TofIS=e-UgM{(ZHn0Hw}i$ zl1(L3G(CLkWc*4n%#zt)6fIjK#CMWDIp%u?6GYQT1^8 zhMP=;AGSM?aN`GWpc$J~u92Frk!c|+FaFn{2=6wi>qKz{qwd_MCf=UA&U%=14Q!Ax nd#D;Ag4K#h(tC~MF(nSc`}l+_yAAnxg?{)8SJKxTL<0Z-{K&>r diff --git a/tests/test_ncbi_parse_genomic_gff.py b/tests/test_ncbi_parse_genomic_gff.py index 7f66b0d..3e0a816 100644 --- a/tests/test_ncbi_parse_genomic_gff.py +++ b/tests/test_ncbi_parse_genomic_gff.py @@ -23,7 +23,7 @@ def sample_line(**params): "start": 11874, "stop": 12227, "score": ".", - "strand": "+", + "strand": "1", "phase": ".", "attributes_str": "ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=NR_046018.2", } @@ -86,7 +86,7 @@ def test_parse_gff_record(self): seqid="NC_000001.10", start=11874, end=12227, - strand="+", + strand="1", exon_number=1, parent_id="rna-NR_046018.2", transcript_id="NR_046018.2", From e5edee5cfa55a75a1604b778dfd4c439025b1356 Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Thu, 7 Mar 2024 12:20:32 -0800 Subject: [PATCH 09/54] feat(IPVC-2231): address PR feedback --- sbin/ncbi-parse-gff | 443 --------------------------- sbin/ncbi_parse_genomic_gff.py | 17 +- src/uta/loading.py | 2 +- src/uta/models.py | 2 +- tests/test_ncbi_parse_genomic_gff.py | 7 - 5 files changed, 12 insertions(+), 459 deletions(-) delete mode 100755 sbin/ncbi-parse-gff diff --git a/sbin/ncbi-parse-gff b/sbin/ncbi-parse-gff deleted file mode 100755 index 4a34ed2..0000000 --- a/sbin/ncbi-parse-gff +++ /dev/null @@ -1,443 +0,0 @@ -#!/usr/bin/env python - -"""Write exonsets and txinfo files from NCBI GFF alignments, as obtained from -ftp://ftp.ncbi.nlm.nih.gov/refseq/H_sapiens/alignments/ -This service appeared in April 2015 and is due to update weekly. - -See uta.formats for a description of those file formats. - -In a nutshell, this means that you'll get data like this: - -ncbi.txinfo.gz: -origin ac hgnc cds_se_i exons_se_i -NCBI RefSeq NM_053283.2 DCD 62,395 0,120;120,159;159,261;261,351;351,517 - -ncbi.exonsets.gz: -tx_ac alt_ac method strand exons_se_i -NM_130786.3 NC_000019.9 splign -1 58864769,58864865;588646... -NM_130786.3 NC_018930.2 splign -1 58858699,58858795;588585... -NM_130786.3 AC_000151.1 splign -1 55173924,55174020;551738... -NM_138933.2 NC_000010.10 splign -1 52645340,52645435;52... - -UTA requires that the exon structure of a transcript accession as -defined on its own sequence is unique. Although this is mostly true, -there are instances where NCBI reports different exon structures for a -single transcript. For example, NM_001300954.1 aligns with 11 exons on -NC_000011.9 and 5 exons on NW_003871081.1, and the differences are NOT -due merely to concatenation of adjacent spans. This script warns -vaguely about this problem - -""" - - -from __future__ import division - -import argparse -import collections -import gzip -import io -import itertools -import logging.config -import os -import pprint -import pkg_resources -import re -import sys - -import attr -import prettytable - -from uta.formats.exonset import ExonSet, ExonSetWriter -from uta.formats.txinfo import TxInfo, TxInfoWriter, TxInfoReader -from uta.formats.geneaccessions import GeneAccessionsReader - -origin = "NCBI" - - -@attr.s(slots=True) -class ExonAlignment(object): - """alignment of a single exon""" - ref_ac = attr.ib() - origin = attr.ib() - match_type = attr.ib() - g_start = attr.ib(converter=int) - g_end = attr.ib(converter=int) - score = attr.ib() - strand = attr.ib() - aln_id = attr.ib() - tx_ac = attr.ib() - tx_start = attr.ib(converter=int) - tx_end = attr.ib(converter=int) - pct_coverage = attr.ib(converter=float) - pct_identity_gap = attr.ib(converter=float) - pct_identity_ungap = attr.ib(converter=float) - - -@attr.s(slots=True) -class TranscriptAlignment(object): - """Represents a set of ExonAlignments for a single alignment id in tx exon start order""" - - exon_alignments = attr.ib() - - def __getitem__(self, sl): - return self.exon_alignments.__getitem__(sl) - - def __len__(self): - return len(self.exon_alignments) - - @property - def tx_exons_se_i(self): - return ";".join("{s},{e}".format(s=ea.tx_start-1, e=ea.tx_end) for ea in self.exon_alignments) - - @property - def ref_exons_se_i(self): - return ";".join("{s},{e}".format(s=ea.g_start-1, e=ea.g_end) for ea in self.exon_alignments) - - @property - def tx_lens(self): - return [(ea.tx_end-ea.tx_start+1) for ea in self.exon_alignments] - - @property - def ref_lens(self): - return [(ea.g_end-ea.g_start+1) for ea in self.exon_alignments] - - @property - def ref_ac(self): - return self.exon_alignments[0].ref_ac - - @property - def origin(self): - return self.exon_alignments[0].origin - - @property - def score(self): - return self.exon_alignments[0].score - - @property - def strand(self): - return self.exon_alignments[0].strand - - @property - def aln_id(self): - return self.exon_alignments[0].aln_id - - @property - def tx_ac(self): - return self.exon_alignments[0].tx_ac - - @property - def pct_coverage(self): - return self.exon_alignments[0].pct_coverage - - @property - def pct_identity_gap(self): - return self.exon_alignments[0].pct_identity_gap - - @property - def pct_identity_ungap(self): - return self.exon_alignments[0].pct_identity_ungap - - -def parse_args(argv): - ap = argparse.ArgumentParser( - description=__doc__, - ) - ap.add_argument("in_fn") - ap.add_argument("--origin", "-o", - default=origin) - ap.add_argument("--prefix", "-p", - default="ncbi-gff") - ap.add_argument("--geneacs", "-G") - ap.add_argument("--txinfo", "-T", required=False) - ap.add_argument("--strict-coverage", "-C", type=float, default=95.0) - ap.add_argument("--min-coverage", "-c", type=float, default=85.0) - ap.add_argument("--strict-pct-identity-gap", "-I", type=float, default=95.0) - ap.add_argument("--min-pct-identity-gap", "-i", type=float, default=85.0) - - opts = ap.parse_args(argv) - - assert opts.strict_coverage > opts.min_coverage - assert opts.strict_pct_identity_gap > opts.min_pct_identity_gap - - return opts - - -def read_exon_alignments(fn): - """read lines of NCBI's alignment gff file, fn, returning ExonAlignment records""" - - # NC_000007.13 RefSeq cDNA_match 50344265 50344518 254 + . ID=aln58042;Target=NM_001220765.2 1 254 +;gap_count=0;identity=0.0691326;idty=1;num_ident=428;num_mismatch=0;pct_coverage=6.91326;pct_identity_gap=100;pct_identity_ungap=100;score=254 - # NC_000002.11 RefSeq cDNA_match 179671939 179672150 212 - . ID=ed951d46-194c-477a-a480-4bc64530c5ba;Target=NM_001267550.2 1 212 +;gap_count=0;identity=0.999991;idty=1;num_ident=109223;num_mismatch=1;pct_coverage=100;pct_identity_gap=99.9991;pct_identity_ungap=99.9991 - line_re = re.compile( - "(?P\S+)\s+(?P\S+)\s+(?P\S+)\s+" - "(?P\d+)\s+(?P\d+)\s+(?P\S+)\s+" - "(?P[-+])\s+\.\s+ID=(?P[^;]+);Target=(?P\S+)" - "\s+(?P\d+)\s+(?P\d+).+?" - "pct_coverage=(?P[^;]+);" - "pct_identity_gap=(?P[^;]+);" - "pct_identity_ungap=(?P[^;]+)" - ) - fh = io.open(fn, "rt") - for line in fh.readlines(): - if not line.startswith('#'): - try: - yield ExonAlignment(**line_re.match(line).groupdict()) - except (AttributeError, ValueError): - raise Exception("Failed at", line) - - -def read_transcript_alignments(fn): - """read an NCBI alignment gff, returning a generator of TranscriptAlignments - that contains alignments grouped on id and sorted by tx_start - - This code assumes that alignments with the same alignment id occur - in contiguous blocks in the file. - - """ - - exon_alignments = read_exon_alignments(fn) - return [TranscriptAlignment(exon_alignments=sorted(exons_i, key=lambda e: e.tx_start)) - for _, exons_i in itertools.groupby(exon_alignments, key=lambda e: e.aln_id)] - - -def group_transcript_alignments(transcript_alignments): - """group transcript_alignments by tx_ac and ref_ac - - IMPORTANT: The gff file may contain multiple alignments for a - pair. These pairs are not necessarily adjacent in - the file. Therefore, the source must be materialized in order to - sort and group transcript_alignments. - - """ - - def _key(e): - return (e.tx_ac, e.ref_ac) - transcript_alignments = list(transcript_alignments) - transcript_alignments.sort(key=_key) - return ((key, list(alns_i)) - for key, alns_i in itertools.groupby(transcript_alignments, key=_key)) - - -def convert_exon_data(opts, transcript_alignment): - """return (TxInfo,ExonSet) tuple for given exon record data""" - ti = TxInfo(ac=transcript_alignment.tx_ac, - origin=opts.origin, - hgnc=None, - cds_se_i=None, - exons_se_i=transcript_alignment.tx_exons_se_i - ) - es = ExonSet( - tx_ac=transcript_alignment.tx_ac, - alt_ac=transcript_alignment.ref_ac, - method="splign", - strand=-1 if transcript_alignment.strand == "-" else 1, - exons_se_i=transcript_alignment.ref_exons_se_i - ) - return (ti, es) - - -if __name__ == "__main__": - logging_conf_fn = pkg_resources.resource_filename( - "uta", "etc/logging.conf") - logging.config.fileConfig(logging_conf_fn) - logging.getLogger().setLevel(logging.INFO) - logger = logging.getLogger(__name__) - - opts = parse_args(sys.argv[1:]) - - if opts.geneacs: - gar = GeneAccessionsReader(gzip.open(opts.geneacs, "rt")) - tx2gene = {ga.tx_ac: ga.hgnc for ga in gar} - logger.info( - "read {} gene-accession mappings from {}".format(len(tx2gene), opts.geneacs)) - else: - tx2gene = None - logger.info("No geneacs (-G) file provided; gene info will be empty.") - - if opts.txinfo: - tir = TxInfoReader(gzip.open(opts.txinfo, "rt")) - tx2ti = {ti.ac: ti for ti in tir} - logger.info( - "read {} CDS data from {}".format(len(tx2ti), opts.txinfo)) - # add any gene-accession mappings from txinfo file if they are not in geneacs file; log warning if they disagree - if tx2gene: - for ti_ac in tx2ti: - if not tx2gene.get(ti_ac): - tx2gene[ti_ac] = tx2ti[ti_ac].hgnc - if tx2gene[ti_ac] != tx2ti[ti_ac].hgnc: - logger.warning('HGNC symbol disagrees in txinfo ({tx2ti_hgnc}) and geneacs ({tx2gene_hgnc}) files for accession {ti_ac}'.format( - tx2ti_hgnc=tx2ti[ti_ac].hgnc, - tx2gene_hgnc=tx2gene[ti_ac].hgnc, - ti_ac=ti_ac - )) - - else: - tx2ti = None - logger.info("No gbff txinfo provided (-T); CDS start,end will be undefined for all transcripts and transcript-genome exon structures will not be verified") - - es_fn = opts.prefix + "exonset.gz" - ti_fn = opts.prefix + "txinfo.gz" - - esw = ExonSetWriter(gzip.open(es_fn + ".tmp", "wt")) - tiw = TxInfoWriter(gzip.open(ti_fn + ".tmp", "wt")) - - ties = {} - ti_written = collections.defaultdict(lambda: False) - ac_not_in_gbff = set() - ac_exons_differ = set() - ac_in_source = set() - ac_failed = set() - - bins = "nogbff esdiffer unique multiple minimum none".split() - sets = collections.defaultdict(lambda: {k: list() for k in bins}) - - transcript_alignments = read_transcript_alignments(opts.in_fn) - logger.info( - "read {} transcript alignments from {}".format(len(transcript_alignments), opts.in_fn)) - - for _, txalns in group_transcript_alignments(transcript_alignments): - assert len(txalns) > 0 - - ta0 = txalns[0] - tx_ac, ref_ac = ta0.tx_ac, ta0.ref_ac - skey = "{:.2s} {:.2s}".format(tx_ac, ref_ac) - bin = None - - # ############################################################ - # Optionally compare exon structure from gbff with input gff - # And get cds s,e from gbff (sigh) - if tx2ti is None: - cds_se_i = None - txalns_esm = txalns - else: - if tx_ac not in tx2ti: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: no transcript info in {opts.txinfo}; skipping transcript".format( - ta=ta0, opts=opts)) - ac_not_in_gbff.add(tx_ac) - bin = "nogbff" - sets[skey][bin] += [txalns] - continue - - gbff_ti = tx2ti[tx_ac] - txalns_esm = [ta for ta in txalns if ta.tx_exons_se_i == gbff_ti.exons_se_i] - n_rm = len(txalns) - len(txalns_esm) - if n_rm > 0: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: Removed {n_rm}/{n_tot} exon structures that differ from gbff definition".format( - n_rm=n_rm, n_tot=len(txalns), ta=ta, opts=opts)) - if len(txalns_esm) == 0: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: All {n} exon structures differ from gbff definition; skipping alignment".format( - ta=ta, opts=opts, n=len(txalns))) - ac_exons_differ.add(tx_ac) - bin = "esdiffer" - sets[skey][bin] += [txalns] - continue - - cds_se_i = gbff_ti.cds_se_i # possibly None - - - # ############################################################ - # Filter alignments by coverage and pct_identity_gap - # From Terence Murphy, NCBI: - # "For remapping variation, we typically only use transcript - # and RefSeqGene alignments that meet the filter: - # 'pct_identity_gap >= 99.5 and pct_coverage >= 95'" - txalns_strict = [txaln - for txaln in txalns_esm - if (txaln.pct_coverage > opts.strict_coverage - and txaln.pct_identity_gap > opts.strict_pct_identity_gap)] - - if len(txalns_strict) == 1: - - txalns_load = txalns_strict - bin = "unique" - - elif len(txalns_strict) > 1: - - logger.warning("{ta.tx_ac}~{ta.ref_ac}: Multiple ({n}) strict alignments; cov/pig: {stats}".format( - ta=txalns_strict[0], n=len(txalns_strict), opts=opts, - stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_strict), - )) - txalns_load = txalns_strict - bin = "multiple" - - if len(txalns_strict) == 0: - - txalns_min = [txaln - for txaln in txalns_esm - if (txaln.pct_coverage > opts.min_coverage - and txaln.pct_identity_gap > opts.min_pct_identity_gap)] - if len(txalns_min) == 0: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: No usable alignments; cov/pig: {stats}".format( - ta=txalns[0], - stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_esm), - )) - bin = "none" - else: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: Resorting to minimum criteria; loading {n} alignments; cov/pig: {stats}".format( - ta=txalns_min[0], n=len(txalns_min), - stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_min), - )) - bin = "minimum" - txalns_load = txalns_min - - sets[skey][bin] += [txalns_esm] - - for ta in txalns_load: - ti, es = convert_exon_data(opts, ta) - ti.cds_se_i = cds_se_i - ac_in_source.add(tx_ac) - ti.hgnc = tx2gene.get(ti.ac, None) - - if not ti_written[ti.ac]: - # write a single txinfo line once; multiple may occur for multiple alignments of e.g., one NM to NC, NW, NT - tiw.write(ti) - ti_written[ti.ac] = True - - esw.write(es) - - # END HEINOUS LOOP - - for fn in [ti_fn, es_fn]: - os.rename(fn + ".tmp", fn) - - seen_but_failed = ac_failed - ac_in_source - if seen_but_failed: - logger.warning("{n_acv} acvs seen but failed criteria: {acs}".format( - n_acv=len(seen_but_failed), acs=",".join(sorted(seen_but_failed)))) - - if ac_not_in_gbff: - s_not_g_b = set(k.partition(".")[ - 0] for k in ac_in_source) - set(k.partition(".")[0] for k in tx2gene.keys()) - logger.warning("{n_acv} acvs ({n_ac} base acs) in source not in geneacs file: {acs}".format( - n_acv=len(ac_not_in_gbff), n_ac=len(s_not_g_b), opts=opts, acs=",".join(sorted(ac_not_in_gbff)))) - - if ac_exons_differ: - logger.warning("{n} accessions in gbff-derived txinfo have different exon coordinates: {acs}".format( - n=len(ac_exons_differ), opts=opts, acs=",".join(sorted(ac_exons_differ)))) - - pprint.pprint(opts) - pt = prettytable.PrettyTable(field_names=["ac_pair"] - + bins - + "max_coverage max_pct_identity_gap nobgffs nogbff_noup esdiffers nones".split() - ) - for ack in sorted(sets.keys()): - n = 5 - nogbff_acs = sorted(set(ta.tx_ac for ta in itertools.chain.from_iterable(sets[ack]["nogbff"])))[:n] - esdiffer_acs = sorted(set(ta.tx_ac for ta in itertools.chain.from_iterable(sets[ack]["esdiffer"])))[:n] - nones = list(itertools.chain.from_iterable(sets[ack]["none"])) - nones_acs = sorted(set(ta.tx_ac for ta in nones))[:n] - max_pct_identity_gap = "{:.2f}".format(max(ta.pct_identity_gap for ta in nones)) if nones else "n/a" - max_pct_coverage = "{:.2f}".format(max(ta.pct_coverage for ta in nones)) if nones else "n/a" - - nogbff_noup = sorted( - set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["nogbff"])) - - set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["unique"])) - - set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["multiple"])) - ) - - pt.add_row([ack] + [len(sets[ack][bk]) for bk in bins] + - [max_pct_coverage, max_pct_identity_gap, - " ".join(nogbff_acs), - str(len(nogbff_noup)) + ": " + " ".join(nogbff_noup[:n]), - " ".join(esdiffer_acs), - " ".join(nones_acs) ]) - print(pt) diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 9ed3806..05b2371 100644 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -22,19 +22,17 @@ """ import argparse -import os -from typing import List, Optional import gzip +import logging.config +import os from collections import defaultdict from dataclasses import dataclass +from typing import List, Optional -import logging +import pkg_resources from uta.formats.exonset import ExonSet, ExonSetWriter -logging.basicConfig(level=logging.INFO) -logger = logging.getLogger(__name__) - @dataclass class GFFRecord: @@ -63,7 +61,7 @@ def parse_gff_record(line: str) -> Optional[GFFRecord]: seqid, source, feature, start, end, score, strand, phase, attributes_str = fields - if feature != "exon" or not seqid.startswith("NC_"): + if feature != "exon": return attributes = {} @@ -128,6 +126,11 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: if __name__ == "__main__": + logging_conf_fn = pkg_resources.resource_filename("uta", "etc/logging.conf") + logging.config.fileConfig(logging_conf_fn) + logging.getLogger().setLevel(logging.INFO) + logger = logging.getLogger(__name__) + parser = argparse.ArgumentParser(description="Parse GFF file.") parser.add_argument( "gff_file", type=argparse.FileType("rb"), help="Path to the gzipped GFF file" diff --git a/src/uta/loading.py b/src/uta/loading.py index f0dc6fc..ea5c041 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -17,7 +17,7 @@ from sqlalchemy.orm.exc import NoResultFound import psycopg2.extras import six -# import uta_align.align.algorithms as utaaa +import uta_align.align.algorithms as utaaa from uta.lru_cache import lru_cache diff --git a/src/uta/models.py b/src/uta/models.py index 6cd64ed..2305666 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -13,7 +13,7 @@ # schema name support # also see etc/uta.conf -schema_version = "20210129b" +schema_version = "1.1" use_schema = True if use_schema: schema_name = "uta_" + schema_version.replace(".","_") diff --git a/tests/test_ncbi_parse_genomic_gff.py b/tests/test_ncbi_parse_genomic_gff.py index 3e0a816..a8b53b0 100644 --- a/tests/test_ncbi_parse_genomic_gff.py +++ b/tests/test_ncbi_parse_genomic_gff.py @@ -101,13 +101,6 @@ def test_parse_gff_record_skips_non_exon_records(self): parsed_record = parse_gff_record(line) self.assertEqual(parsed_record, expected_record) - def test_parse_gff_record_skips_non_NC_target(self): - # We exclude alignments to non-NC chromosomes - line = sample_line(seqid="NT_123") - expected_record = None - parsed_record = parse_gff_record(line) - self.assertEqual(parsed_record, expected_record) - def test_parse_gff_record_skips_missing_transcript_id(self): # We exclude alignments missing transcript_id line = sample_line( From 8e868503ebae0cdf46243f6139bf768a071cd4b4 Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Mon, 11 Mar 2024 09:41:46 -0700 Subject: [PATCH 10/54] refactor(IPVC-2262): write to stdout, read/write data uncompressed, update tests and test data --- sbin/ncbi_parse_genomic_gff.py | 22 ++--- tests/data/expected_genomic_100.exonset | 12 +++ tests/data/expected_genomic_100.exonset.gz | Bin 392 -> 0 bytes tests/data/genomic_100.gff | 100 +++++++++++++++++++++ tests/data/genomic_100.gff.gz | Bin 3704 -> 0 bytes tests/test_ncbi_parse_genomic_gff.py | 37 +++----- 6 files changed, 127 insertions(+), 44 deletions(-) create mode 100644 tests/data/expected_genomic_100.exonset delete mode 100644 tests/data/expected_genomic_100.exonset.gz create mode 100644 tests/data/genomic_100.gff delete mode 100644 tests/data/genomic_100.gff.gz diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 05b2371..87f6468 100644 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -22,9 +22,8 @@ """ import argparse -import gzip import logging.config -import os +import sys from collections import defaultdict from dataclasses import dataclass from typing import List, Optional @@ -104,7 +103,7 @@ def _get_exon_number_from_id(alignment_id: str) -> int: def parse_gff_file(file_path: str) -> dict[str, List[GFFRecord]]: tx_data = defaultdict(list) - with gzip.open(file_path, "rt") as f: + with open(file_path, "rt") as f: for line in f: if line.startswith("#"): continue @@ -133,25 +132,16 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: parser = argparse.ArgumentParser(description="Parse GFF file.") parser.add_argument( - "gff_file", type=argparse.FileType("rb"), help="Path to the gzipped GFF file" - ) - parser.add_argument( - "--prefix", - "-p", - type=str, - default="ncbi-gff", - help="Output filename (default: ncbi-gff)", + "gff_file", type=str, help="Path to GFF file" ) args = parser.parse_args() gff_infile = args.gff_file - exonset_outfile = args.prefix + ".exonset.gz" - output_file = gzip.open(exonset_outfile + ".tmp", "wt") - esw = ExonSetWriter(output_file) + esw = ExonSetWriter(sys.stdout) transcript_alignments = parse_gff_file(gff_infile) logger.info( - f"read {len(transcript_alignments)} transcript alignments from {gff_infile.name}" + f"read {len(transcript_alignments)} transcript alignments from {gff_infile}" ) for transcript_exons in transcript_alignments.values(): @@ -165,5 +155,3 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: exons_se_i=exons_se, ) esw.write(es) - output_file.close() - os.rename(exonset_outfile + ".tmp", exonset_outfile) diff --git a/tests/data/expected_genomic_100.exonset b/tests/data/expected_genomic_100.exonset new file mode 100644 index 0000000..172f7a0 --- /dev/null +++ b/tests/data/expected_genomic_100.exonset @@ -0,0 +1,12 @@ +tx_ac alt_ac method strand exons_se_i +NR_046018.2 NC_000001.10 splign 1 11873,12227;12612,12721;13220,14409 +NR_024540.1 NC_000001.10 splign -1 29320,29370;24737,24891;18267,18366;17914,18061;17605,17742;17232,17368;16857,17055;16606,16765;15795,15947;14969,15038;14361,14829 +NR_106918.1 NC_000001.10 splign -1 17368,17436 +NR_036051.1 NC_000001.10 splign 1 30365,30503 +NR_026818.1 NC_000001.10 splign -1 35720,36081;35276,35481;34610,35174 +NM_001005484.2 NC_000001.10 splign 1 65418,65433;65519,65573;69036,71585 +NR_039983.2 NC_000001.10 splign -1 140074,140566;139789,139847;134772,139696 +NR_028322.1 NC_000001.10 splign 1 323891,324060;324287,324345;324438,328581 +NM_001005221.2 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zG+jk|+4uhxy#|b|CZP%p_Uj)<;|bt6C_AC0kDy~G^V@hFPDaI_w=+BIht|%ruUFLF zr3POuyXdo`xfl5_=@JMDlbg{jTQSn$*C5KyR(-wB1A|G!;pHP?9%E6LGzj>PW^*xx z2D8T69cPHq#>EdBNQUa`V3{2EZLsW<>oQpWAu{iRZF9-11zu^i1i>tDT=VKgty{7} zz@|*-b#`8J${Jwba>|lzlUImbXW5-pRF=IEV*6=jgP)d<-w}R($4dcczH=y4Rc?vg zxDCZ1s3jXiH*P~UAXk^6RO-5Jb;7Eyc_|6@Ew9c2{xx8Ans*;lsa`%dBY9G Date: Mon, 11 Mar 2024 14:09:42 -0600 Subject: [PATCH 11/54] feat(IPVC-2188): add Dockerfile to repo and upgrade uta-align (#2) --- Dockerfile | 24 ++ README.md | 43 +++ archive/ncbi-parse-gff | 443 +++++++++++++++++++++++++++++++ etc/scripts/create-new-schema.sh | 24 ++ etc/scripts/delete-schema.sh | 14 + etc/scripts/run-uta-build.sh | 51 ++++ pyproject.toml | 2 +- src/uta/loading.py | 87 +++--- 8 files changed, 644 insertions(+), 44 deletions(-) create mode 100644 Dockerfile create mode 100755 archive/ncbi-parse-gff create mode 100755 etc/scripts/create-new-schema.sh create mode 100755 etc/scripts/delete-schema.sh create mode 100644 etc/scripts/run-uta-build.sh diff --git a/Dockerfile b/Dockerfile new file mode 100644 index 0000000..6718976 --- /dev/null +++ b/Dockerfile @@ -0,0 +1,24 @@ +FROM ubuntu:22.04 + +# set python version and define arguments +ARG python_version="3.10" + +# list and install dependencies +ARG dependencies="python${python_version} python3-dev python3-pip rsync git postgresql-client-14" + +RUN apt-get update && apt-get install -y $dependencies && apt-get clean + +# install pysam, copy code, and run pip install +RUN ln -s /usr/bin/python3 /usr/bin/python +RUN python -m pip install --upgrade pip +RUN pip install --upgrade setuptools +RUN pip install pysam + +ENV APP_HOME="/opt/repos/uta/" + +RUN mkdir -p ${APP_HOME} + +COPY . ${APP_HOME} +WORKDIR ${APP_HOME} + +RUN pip install -e .[dev] diff --git a/README.md b/README.md index b017f66..d286edb 100644 --- a/README.md +++ b/README.md @@ -251,6 +251,7 @@ the installation environment.* ## Developer Setup +### Virtual Environment To develop UTA, follow these steps. 1. Set up a virtual environment using your preferred method. @@ -270,3 +271,45 @@ To develop UTA, follow these steps. 4. To run the tests: $ python3 -m unittest + +### Docker + +1. Clone UTA and build docker image: + + $ git clone git@github.com:biocommons/uta.git + $ cd uta + $ docker build -t uta . + +2. Restore a database or load a new one using the instructions [above](#installing-from-database-dumps). + +3. Run container and tests + + $ docker run -it --rm uta-build uta:latest + $ python -m unittest + +## Running local UTA build + +### Docker +To run local build of UTA you can follow these steps... + +1. Pick a UTA version as the base (ex. "uta_20210129b") + +2. Setup local database follow the steps above (`Installing with Docker`) to pull image, + start and populate. __NOTE__...it does take about 5 minutes for the local database is ready. + +3. Build the image + + $ docker build -t uta . + +4. Run UTA build. Supplying a local path with data needed for the workflow is a requirement. + + $ docker run \ + -it \ + --rm \ + --name uta-build \ + --volume .:/opt/repos/uta \ + --volume /System/Volumes/Data/locus/data/test-uta-22:/temp \ + --network=host uta-build:latest + +5. Once in the container you can run this script to create new schema, process input files, + update the database, and dump a new artifact. diff --git a/archive/ncbi-parse-gff b/archive/ncbi-parse-gff new file mode 100755 index 0000000..4a34ed2 --- /dev/null +++ b/archive/ncbi-parse-gff @@ -0,0 +1,443 @@ +#!/usr/bin/env python + +"""Write exonsets and txinfo files from NCBI GFF alignments, as obtained from +ftp://ftp.ncbi.nlm.nih.gov/refseq/H_sapiens/alignments/ +This service appeared in April 2015 and is due to update weekly. + +See uta.formats for a description of those file formats. + +In a nutshell, this means that you'll get data like this: + +ncbi.txinfo.gz: +origin ac hgnc cds_se_i exons_se_i +NCBI RefSeq NM_053283.2 DCD 62,395 0,120;120,159;159,261;261,351;351,517 + +ncbi.exonsets.gz: +tx_ac alt_ac method strand exons_se_i +NM_130786.3 NC_000019.9 splign -1 58864769,58864865;588646... +NM_130786.3 NC_018930.2 splign -1 58858699,58858795;588585... +NM_130786.3 AC_000151.1 splign -1 55173924,55174020;551738... +NM_138933.2 NC_000010.10 splign -1 52645340,52645435;52... + +UTA requires that the exon structure of a transcript accession as +defined on its own sequence is unique. Although this is mostly true, +there are instances where NCBI reports different exon structures for a +single transcript. For example, NM_001300954.1 aligns with 11 exons on +NC_000011.9 and 5 exons on NW_003871081.1, and the differences are NOT +due merely to concatenation of adjacent spans. This script warns +vaguely about this problem + +""" + + +from __future__ import division + +import argparse +import collections +import gzip +import io +import itertools +import logging.config +import os +import pprint +import pkg_resources +import re +import sys + +import attr +import prettytable + +from uta.formats.exonset import ExonSet, ExonSetWriter +from uta.formats.txinfo import TxInfo, TxInfoWriter, TxInfoReader +from uta.formats.geneaccessions import GeneAccessionsReader + +origin = "NCBI" + + +@attr.s(slots=True) +class ExonAlignment(object): + """alignment of a single exon""" + ref_ac = attr.ib() + origin = attr.ib() + match_type = attr.ib() + g_start = attr.ib(converter=int) + g_end = attr.ib(converter=int) + score = attr.ib() + strand = attr.ib() + aln_id = attr.ib() + tx_ac = attr.ib() + tx_start = attr.ib(converter=int) + tx_end = attr.ib(converter=int) + pct_coverage = attr.ib(converter=float) + pct_identity_gap = attr.ib(converter=float) + pct_identity_ungap = attr.ib(converter=float) + + +@attr.s(slots=True) +class TranscriptAlignment(object): + """Represents a set of ExonAlignments for a single alignment id in tx exon start order""" + + exon_alignments = attr.ib() + + def __getitem__(self, sl): + return self.exon_alignments.__getitem__(sl) + + def __len__(self): + return len(self.exon_alignments) + + @property + def tx_exons_se_i(self): + return ";".join("{s},{e}".format(s=ea.tx_start-1, e=ea.tx_end) for ea in self.exon_alignments) + + @property + def ref_exons_se_i(self): + return ";".join("{s},{e}".format(s=ea.g_start-1, e=ea.g_end) for ea in self.exon_alignments) + + @property + def tx_lens(self): + return [(ea.tx_end-ea.tx_start+1) for ea in self.exon_alignments] + + @property + def ref_lens(self): + return [(ea.g_end-ea.g_start+1) for ea in self.exon_alignments] + + @property + def ref_ac(self): + return self.exon_alignments[0].ref_ac + + @property + def origin(self): + return self.exon_alignments[0].origin + + @property + def score(self): + return self.exon_alignments[0].score + + @property + def strand(self): + return self.exon_alignments[0].strand + + @property + def aln_id(self): + return self.exon_alignments[0].aln_id + + @property + def tx_ac(self): + return self.exon_alignments[0].tx_ac + + @property + def pct_coverage(self): + return self.exon_alignments[0].pct_coverage + + @property + def pct_identity_gap(self): + return self.exon_alignments[0].pct_identity_gap + + @property + def pct_identity_ungap(self): + return self.exon_alignments[0].pct_identity_ungap + + +def parse_args(argv): + ap = argparse.ArgumentParser( + description=__doc__, + ) + ap.add_argument("in_fn") + ap.add_argument("--origin", "-o", + default=origin) + ap.add_argument("--prefix", "-p", + default="ncbi-gff") + ap.add_argument("--geneacs", "-G") + ap.add_argument("--txinfo", "-T", required=False) + ap.add_argument("--strict-coverage", "-C", type=float, default=95.0) + ap.add_argument("--min-coverage", "-c", type=float, default=85.0) + ap.add_argument("--strict-pct-identity-gap", "-I", type=float, default=95.0) + ap.add_argument("--min-pct-identity-gap", "-i", type=float, default=85.0) + + opts = ap.parse_args(argv) + + assert opts.strict_coverage > opts.min_coverage + assert opts.strict_pct_identity_gap > opts.min_pct_identity_gap + + return opts + + +def read_exon_alignments(fn): + """read lines of NCBI's alignment gff file, fn, returning ExonAlignment records""" + + # NC_000007.13 RefSeq cDNA_match 50344265 50344518 254 + . ID=aln58042;Target=NM_001220765.2 1 254 +;gap_count=0;identity=0.0691326;idty=1;num_ident=428;num_mismatch=0;pct_coverage=6.91326;pct_identity_gap=100;pct_identity_ungap=100;score=254 + # NC_000002.11 RefSeq cDNA_match 179671939 179672150 212 - . ID=ed951d46-194c-477a-a480-4bc64530c5ba;Target=NM_001267550.2 1 212 +;gap_count=0;identity=0.999991;idty=1;num_ident=109223;num_mismatch=1;pct_coverage=100;pct_identity_gap=99.9991;pct_identity_ungap=99.9991 + line_re = re.compile( + "(?P\S+)\s+(?P\S+)\s+(?P\S+)\s+" + "(?P\d+)\s+(?P\d+)\s+(?P\S+)\s+" + "(?P[-+])\s+\.\s+ID=(?P[^;]+);Target=(?P\S+)" + "\s+(?P\d+)\s+(?P\d+).+?" + "pct_coverage=(?P[^;]+);" + "pct_identity_gap=(?P[^;]+);" + "pct_identity_ungap=(?P[^;]+)" + ) + fh = io.open(fn, "rt") + for line in fh.readlines(): + if not line.startswith('#'): + try: + yield ExonAlignment(**line_re.match(line).groupdict()) + except (AttributeError, ValueError): + raise Exception("Failed at", line) + + +def read_transcript_alignments(fn): + """read an NCBI alignment gff, returning a generator of TranscriptAlignments + that contains alignments grouped on id and sorted by tx_start + + This code assumes that alignments with the same alignment id occur + in contiguous blocks in the file. + + """ + + exon_alignments = read_exon_alignments(fn) + return [TranscriptAlignment(exon_alignments=sorted(exons_i, key=lambda e: e.tx_start)) + for _, exons_i in itertools.groupby(exon_alignments, key=lambda e: e.aln_id)] + + +def group_transcript_alignments(transcript_alignments): + """group transcript_alignments by tx_ac and ref_ac + + IMPORTANT: The gff file may contain multiple alignments for a + pair. These pairs are not necessarily adjacent in + the file. Therefore, the source must be materialized in order to + sort and group transcript_alignments. + + """ + + def _key(e): + return (e.tx_ac, e.ref_ac) + transcript_alignments = list(transcript_alignments) + transcript_alignments.sort(key=_key) + return ((key, list(alns_i)) + for key, alns_i in itertools.groupby(transcript_alignments, key=_key)) + + +def convert_exon_data(opts, transcript_alignment): + """return (TxInfo,ExonSet) tuple for given exon record data""" + ti = TxInfo(ac=transcript_alignment.tx_ac, + origin=opts.origin, + hgnc=None, + cds_se_i=None, + exons_se_i=transcript_alignment.tx_exons_se_i + ) + es = ExonSet( + tx_ac=transcript_alignment.tx_ac, + alt_ac=transcript_alignment.ref_ac, + method="splign", + strand=-1 if transcript_alignment.strand == "-" else 1, + exons_se_i=transcript_alignment.ref_exons_se_i + ) + return (ti, es) + + +if __name__ == "__main__": + logging_conf_fn = pkg_resources.resource_filename( + "uta", "etc/logging.conf") + logging.config.fileConfig(logging_conf_fn) + logging.getLogger().setLevel(logging.INFO) + logger = logging.getLogger(__name__) + + opts = parse_args(sys.argv[1:]) + + if opts.geneacs: + gar = GeneAccessionsReader(gzip.open(opts.geneacs, "rt")) + tx2gene = {ga.tx_ac: ga.hgnc for ga in gar} + logger.info( + "read {} gene-accession mappings from {}".format(len(tx2gene), opts.geneacs)) + else: + tx2gene = None + logger.info("No geneacs (-G) file provided; gene info will be empty.") + + if opts.txinfo: + tir = TxInfoReader(gzip.open(opts.txinfo, "rt")) + tx2ti = {ti.ac: ti for ti in tir} + logger.info( + "read {} CDS data from {}".format(len(tx2ti), opts.txinfo)) + # add any gene-accession mappings from txinfo file if they are not in geneacs file; log warning if they disagree + if tx2gene: + for ti_ac in tx2ti: + if not tx2gene.get(ti_ac): + tx2gene[ti_ac] = tx2ti[ti_ac].hgnc + if tx2gene[ti_ac] != tx2ti[ti_ac].hgnc: + logger.warning('HGNC symbol disagrees in txinfo ({tx2ti_hgnc}) and geneacs ({tx2gene_hgnc}) files for accession {ti_ac}'.format( + tx2ti_hgnc=tx2ti[ti_ac].hgnc, + tx2gene_hgnc=tx2gene[ti_ac].hgnc, + ti_ac=ti_ac + )) + + else: + tx2ti = None + logger.info("No gbff txinfo provided (-T); CDS start,end will be undefined for all transcripts and transcript-genome exon structures will not be verified") + + es_fn = opts.prefix + "exonset.gz" + ti_fn = opts.prefix + "txinfo.gz" + + esw = ExonSetWriter(gzip.open(es_fn + ".tmp", "wt")) + tiw = TxInfoWriter(gzip.open(ti_fn + ".tmp", "wt")) + + ties = {} + ti_written = collections.defaultdict(lambda: False) + ac_not_in_gbff = set() + ac_exons_differ = set() + ac_in_source = set() + ac_failed = set() + + bins = "nogbff esdiffer unique multiple minimum none".split() + sets = collections.defaultdict(lambda: {k: list() for k in bins}) + + transcript_alignments = read_transcript_alignments(opts.in_fn) + logger.info( + "read {} transcript alignments from {}".format(len(transcript_alignments), opts.in_fn)) + + for _, txalns in group_transcript_alignments(transcript_alignments): + assert len(txalns) > 0 + + ta0 = txalns[0] + tx_ac, ref_ac = ta0.tx_ac, ta0.ref_ac + skey = "{:.2s} {:.2s}".format(tx_ac, ref_ac) + bin = None + + # ############################################################ + # Optionally compare exon structure from gbff with input gff + # And get cds s,e from gbff (sigh) + if tx2ti is None: + cds_se_i = None + txalns_esm = txalns + else: + if tx_ac not in tx2ti: + logger.warning("{ta.tx_ac}~{ta.ref_ac}: no transcript info in {opts.txinfo}; skipping transcript".format( + ta=ta0, opts=opts)) + ac_not_in_gbff.add(tx_ac) + bin = "nogbff" + sets[skey][bin] += [txalns] + continue + + gbff_ti = tx2ti[tx_ac] + txalns_esm = [ta for ta in txalns if ta.tx_exons_se_i == gbff_ti.exons_se_i] + n_rm = len(txalns) - len(txalns_esm) + if n_rm > 0: + logger.warning("{ta.tx_ac}~{ta.ref_ac}: Removed {n_rm}/{n_tot} exon structures that differ from gbff definition".format( + n_rm=n_rm, n_tot=len(txalns), ta=ta, opts=opts)) + if len(txalns_esm) == 0: + logger.warning("{ta.tx_ac}~{ta.ref_ac}: All {n} exon structures differ from gbff definition; skipping alignment".format( + ta=ta, opts=opts, n=len(txalns))) + ac_exons_differ.add(tx_ac) + bin = "esdiffer" + sets[skey][bin] += [txalns] + continue + + cds_se_i = gbff_ti.cds_se_i # possibly None + + + # ############################################################ + # Filter alignments by coverage and pct_identity_gap + # From Terence Murphy, NCBI: + # "For remapping variation, we typically only use transcript + # and RefSeqGene alignments that meet the filter: + # 'pct_identity_gap >= 99.5 and pct_coverage >= 95'" + txalns_strict = [txaln + for txaln in txalns_esm + if (txaln.pct_coverage > opts.strict_coverage + and txaln.pct_identity_gap > opts.strict_pct_identity_gap)] + + if len(txalns_strict) == 1: + + txalns_load = txalns_strict + bin = "unique" + + elif len(txalns_strict) > 1: + + logger.warning("{ta.tx_ac}~{ta.ref_ac}: Multiple ({n}) strict alignments; cov/pig: {stats}".format( + ta=txalns_strict[0], n=len(txalns_strict), opts=opts, + stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_strict), + )) + txalns_load = txalns_strict + bin = "multiple" + + if len(txalns_strict) == 0: + + txalns_min = [txaln + for txaln in txalns_esm + if (txaln.pct_coverage > opts.min_coverage + and txaln.pct_identity_gap > opts.min_pct_identity_gap)] + if len(txalns_min) == 0: + logger.warning("{ta.tx_ac}~{ta.ref_ac}: No usable alignments; cov/pig: {stats}".format( + ta=txalns[0], + stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_esm), + )) + bin = "none" + else: + logger.warning("{ta.tx_ac}~{ta.ref_ac}: Resorting to minimum criteria; loading {n} alignments; cov/pig: {stats}".format( + ta=txalns_min[0], n=len(txalns_min), + stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_min), + )) + bin = "minimum" + txalns_load = txalns_min + + sets[skey][bin] += [txalns_esm] + + for ta in txalns_load: + ti, es = convert_exon_data(opts, ta) + ti.cds_se_i = cds_se_i + ac_in_source.add(tx_ac) + ti.hgnc = tx2gene.get(ti.ac, None) + + if not ti_written[ti.ac]: + # write a single txinfo line once; multiple may occur for multiple alignments of e.g., one NM to NC, NW, NT + tiw.write(ti) + ti_written[ti.ac] = True + + esw.write(es) + + # END HEINOUS LOOP + + for fn in [ti_fn, es_fn]: + os.rename(fn + ".tmp", fn) + + seen_but_failed = ac_failed - ac_in_source + if seen_but_failed: + logger.warning("{n_acv} acvs seen but failed criteria: {acs}".format( + n_acv=len(seen_but_failed), acs=",".join(sorted(seen_but_failed)))) + + if ac_not_in_gbff: + s_not_g_b = set(k.partition(".")[ + 0] for k in ac_in_source) - set(k.partition(".")[0] for k in tx2gene.keys()) + logger.warning("{n_acv} acvs ({n_ac} base acs) in source not in geneacs file: {acs}".format( + n_acv=len(ac_not_in_gbff), n_ac=len(s_not_g_b), opts=opts, acs=",".join(sorted(ac_not_in_gbff)))) + + if ac_exons_differ: + logger.warning("{n} accessions in gbff-derived txinfo have different exon coordinates: {acs}".format( + n=len(ac_exons_differ), opts=opts, acs=",".join(sorted(ac_exons_differ)))) + + pprint.pprint(opts) + pt = prettytable.PrettyTable(field_names=["ac_pair"] + + bins + + "max_coverage max_pct_identity_gap nobgffs nogbff_noup esdiffers nones".split() + ) + for ack in sorted(sets.keys()): + n = 5 + nogbff_acs = sorted(set(ta.tx_ac for ta in itertools.chain.from_iterable(sets[ack]["nogbff"])))[:n] + esdiffer_acs = sorted(set(ta.tx_ac for ta in itertools.chain.from_iterable(sets[ack]["esdiffer"])))[:n] + nones = list(itertools.chain.from_iterable(sets[ack]["none"])) + nones_acs = sorted(set(ta.tx_ac for ta in nones))[:n] + max_pct_identity_gap = "{:.2f}".format(max(ta.pct_identity_gap for ta in nones)) if nones else "n/a" + max_pct_coverage = "{:.2f}".format(max(ta.pct_coverage for ta in nones)) if nones else "n/a" + + nogbff_noup = sorted( + set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["nogbff"])) + - set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["unique"])) + - set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["multiple"])) + ) + + pt.add_row([ack] + [len(sets[ack][bk]) for bk in bins] + + [max_pct_coverage, max_pct_identity_gap, + " ".join(nogbff_acs), + str(len(nogbff_noup)) + ": " + " ".join(nogbff_noup[:n]), + " ".join(esdiffer_acs), + " ".join(nones_acs) ]) + print(pt) diff --git a/etc/scripts/create-new-schema.sh b/etc/scripts/create-new-schema.sh new file mode 100755 index 0000000..d13100a --- /dev/null +++ b/etc/scripts/create-new-schema.sh @@ -0,0 +1,24 @@ +#!/bin/bash + +if [ "$#" -lt 2 ] +then + echo "error: too few arguments, you provided $#, 1 required" + echo "usage: create-new-schema.sh " + exit 1 +fi + +set -euxo pipefail + +source_uta_v=$1 +dest_uta_v=$2 +dumps_dir=/temp/dumps +mkdir -p $dumps_dir + +# dump current version +pg_dump -U uta_admin -h localhost -d uta -n "$source_uta_v" | \ + gzip -c > $dumps_dir/"$source_uta_v".pgd.gz + +# create new schema +gzip -cdq $dumps_dir/"$source_uta_v".pgd.gz | \ + sbin/pg-dump-schema-rename "$source_uta_v" "$dest_uta_v" | \ + psql -U uta_admin -h localhost -d uta -aeE diff --git a/etc/scripts/delete-schema.sh b/etc/scripts/delete-schema.sh new file mode 100755 index 0000000..a318799 --- /dev/null +++ b/etc/scripts/delete-schema.sh @@ -0,0 +1,14 @@ +#!/bin/bash + +if [ "$#" -lt 1 ] +then + echo "error: too few arguments, you provided $#, 1 required" + echo "usage: delete-schema.sh " + exit 1 +fi + +set -euxo pipefail + +source_uta_v=$1 + +psql -h localhost -U uta_admin -d uta -c "DROP SCHEMA IF EXISTS $source_uta_v CASCADE" diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh new file mode 100644 index 0000000..9052541 --- /dev/null +++ b/etc/scripts/run-uta-build.sh @@ -0,0 +1,51 @@ +#!/bin/bash + +if [ "$#" -lt 1 ] +then + echo "error: too few arguments, you provided $#, 1 required" + echo "usage: run_uta_build_gene.sh " + exit 1 +fi +source_uta_v=$1 + +set -euxo pipefail + +# set local variables and create working directories +loading_schema="uta_1_1" +loading_dir="/temp/loading" +dumps_dir="/temp/dumps" +logs_dir="/temp/logs" +for d in "$loading_dir" "$dumps_dir" "$logs_dir"; + do mkdir -p "$d" +done + +## Drop loading schema, and recreate +etc/scripts/delete-schema.sh $loading_schema +etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_schema" + +### extract meta data +# genes +sbin/ncbi-parse-geneinfo /temp/gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz | \ + gzip -c > "$loading_dir/genes.geneinfo.gz" 2>&1 | tee "$logs_dir/ncbi-parse-geneinfo.log" + +# transcript protein associations +sbin/ncbi-parse-gene2refseq /temp/gene/DATA/gene2accession.gz | gzip -c > "$loading_dir/assocacs.gz" 2>&1 | \ + tee "$logs_dir/ncbi-fetch-assoc-acs" + +# parse transcript info from GBFF input files +GBFF_files=$(ls /temp/refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz) +sbin/ncbi-parse-gbff "$GBFF_files" | gzip -c > "$loading_dir/gbff.txinfo.gz" 2>&1 | \ + tee "$logs_dir/ncbi-parse-gbff.log" + + +### update the uta database +# genes +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$loading_dir/genes.geneinfo.gz" 2>&1 | \ + tee "$logs_dir/load-geneinfo.log" + +# transcript info +#uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$loading_dir/gbff.txinfo.gz" 2>&1 | \ +# tee "$logs_dir/load-txinfo.log" + +### psql_dump +pg_dump -U uta_admin -h localhost -d uta -t "$loading_schema.gene" | gzip -c > "$dumps_dir/uta.pgd.gz" diff --git a/pyproject.toml b/pyproject.toml index 7bfc17d..89b2d3d 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -53,7 +53,7 @@ dependencies = [ "pytz", "recordtype", "sqlalchemy", - "uta-align", + "uta-align>=0.3.0a4", ] [project.optional-dependencies] diff --git a/src/uta/loading.py b/src/uta/loading.py index ea5c041..57ca5fa 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -15,9 +15,10 @@ from sqlalchemy.exc import IntegrityError from sqlalchemy import text from sqlalchemy.orm.exc import NoResultFound +from sqlalchemy import text import psycopg2.extras import six -import uta_align.align.algorithms as utaaa +import uta_align as utaa from uta.lru_cache import lru_cache @@ -41,16 +42,16 @@ def align_exons(session, opts, cf): def _get_cursor(con): cur = con.cursor(cursor_factory=psycopg2.extras.NamedTupleCursor) - cur.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - cur.execute("set search_path = " + usam.schema_name) + cur.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + cur.execute(text("set search_path = " + usam.schema_name)) return cur def align(s1, s2): - score, cigar = utaaa.needleman_wunsch_gotoh_align(s1.encode("ascii"), - s2.encode("ascii"), - extended_cigar=True) - tx_aseq, alt_aseq = utaaa.cigar_alignment( + score, cigar = utaa.algorithms.needleman_wunsch_gotoh_align(s1.encode("ascii"), + s2.encode("ascii"), + extended_cigar=True) + tx_aseq, alt_aseq = utaa.algorithms.cigar_alignment( tx_seq, alt_seq, cigar, hide_match=False) return tx_aseq.decode("ascii"), alt_aseq.decode("ascii"), cigar.to_string().decode("ascii") @@ -151,9 +152,9 @@ def _fetch_seq(ac, s, e): def analyze(session, opts, cf): - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) cmds = [ "analyze verbose" ] @@ -165,13 +166,13 @@ def analyze(session, opts, cf): def create_schema(session, opts, cf): """Create and populate initial schema""" - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) if session.bind.name == "postgresql" and usam.use_schema: - session.execute("create schema " + usam.schema_name) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("create schema " + usam.schema_name)) + session.execute(text("set search_path = " + usam.schema_name)) session.commit() usam.Base.metadata.create_all(session.bind) @@ -188,8 +189,8 @@ def create_schema(session, opts, cf): def drop_schema(session, opts, cf): if session.bind.name == "postgresql" and usam.use_schema: session.execute( - "set role {admin_role};".format(admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + text("set role {admin_role};".format(admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) ddl = "drop schema if exists " + usam.schema_name + " cascade" session.execute(ddl) @@ -202,7 +203,7 @@ def grant_permissions(session, opts, cf): session.execute(text("set role {admin_role};".format( admin_role=cf.get("uta", "admin_role")))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set search_path = " + usam.schema_name)) cmds = [ # alter db doesn't belong here, and probably better to avoid the implicit behevior this encourages @@ -309,9 +310,9 @@ def load_ncbi_geneinfo(session, opts, cf): ftp://ftp.ncbi.nlm.nih.gov/gene/DATA/gene_info.gz """ - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) gip = uta.parsers.geneinfo.GeneInfoParser(gzip.open(opts["FILE"], 'rt')) for gi in gip: @@ -363,9 +364,9 @@ def _seqgene_recs_to_tx_info(ac, assy, recs): return ti - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) o_refseq = session.query(usam.Origin).filter( usam.Origin.name == "NCBI RefSeq").one() @@ -405,9 +406,9 @@ def load_origin(session, opts, cf): def _none_if_empty(s): return None if s == "" else s - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) orir = csv.DictReader(open(opts["FILE"]), delimiter='\t') for rec in orir: @@ -442,9 +443,9 @@ def load_seqinfo(session, opts, cf): max_len = int(2e6) - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) n_rows = len(gzip.open(opts["FILE"]).readlines()) - 1 @@ -522,9 +523,9 @@ def load_sequences(session, opts, cf): # 2e6 was chosen empirically based on sizes of NMs, NGs, NWs, NTs, NCs max_len = int(2e6) - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) sf = _get_seqfetcher(cf) @@ -568,9 +569,9 @@ def _fetch_first(acs): def load_sql(session, opts, cf): """Create views""" - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) for fn in opts["FILES"]: logger.info("loading " + fn) @@ -597,9 +598,9 @@ def _fetch_origin_by_name(name): tir = ufti.TxInfoReader(gzip.open(opts["FILE"], 'rt')) logger.info("opened " + opts["FILE"]) - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) n_new = 0 n_unchanged = 0 @@ -694,9 +695,9 @@ def _fetch_origin_by_name(name): def refresh_matviews(session, opts, cf): - session.execute("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role"))) - session.execute("set search_path = " + usam.schema_name) + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) # matviews must be updated in dependency order. Unfortunately, # it's difficult to determine this programmatically. The "right" From a5df6f34a5ffce4902da51cbe239ff9340cc2b2e Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Mon, 11 Mar 2024 15:08:03 -0700 Subject: [PATCH 12/54] refactor(IPVC-2262): allow input to be compressed or uncompressed, allow input list of files --- sbin/ncbi_parse_genomic_gff.py | 43 ++++++++---- tests/data/genomic_100.gff | 100 --------------------------- tests/data/genomic_100.gff.gz | Bin 0 -> 3720 bytes tests/test_ncbi_parse_genomic_gff.py | 18 ++++- 4 files changed, 43 insertions(+), 118 deletions(-) delete mode 100644 tests/data/genomic_100.gff create mode 100644 tests/data/genomic_100.gff.gz diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 87f6468..9f1f741 100644 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -22,9 +22,11 @@ """ import argparse +import gzip import logging.config import sys from collections import defaultdict +from contextlib import contextmanager from dataclasses import dataclass from typing import List, Optional @@ -33,6 +35,16 @@ from uta.formats.exonset import ExonSet, ExonSetWriter +@contextmanager +def open_file(filename): + if filename.endswith(".gz"): + with gzip.open(filename, "rt") as f: + yield f + else: + with open(filename) as f: + yield f + + @dataclass class GFFRecord: seqid: str @@ -101,18 +113,19 @@ def _get_exon_number_from_id(alignment_id: str) -> int: return int(alignment_id.split("-")[-1]) -def parse_gff_file(file_path: str) -> dict[str, List[GFFRecord]]: +def parse_gff_file(file_paths: List[str]) -> dict[str, List[GFFRecord]]: tx_data = defaultdict(list) - with open(file_path, "rt") as f: - for line in f: - if line.startswith("#"): - continue - try: - record = parse_gff_record(line) - except ValueError as e: - raise Exception(f"Failed at line :{line} with error: {e}") - if record: - tx_data[record.parent_id].append(record) + for file_path in file_paths: + with open_file(file_path) as f: + for line in f: + if line.startswith("#"): + continue + try: + record = parse_gff_record(line) + except ValueError as e: + raise Exception(f"Failed at line :{line} with error: {e}") + if record: + tx_data[record.parent_id].append(record) return {k: _sort_exons(v) for k, v in tx_data.items()} @@ -132,16 +145,16 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: parser = argparse.ArgumentParser(description="Parse GFF file.") parser.add_argument( - "gff_file", type=str, help="Path to GFF file" + "gff_files", nargs='+', type=str, help="Path to GFF file(s)" ) args = parser.parse_args() - gff_infile = args.gff_file + gff_files = args.gff_files esw = ExonSetWriter(sys.stdout) - transcript_alignments = parse_gff_file(gff_infile) + transcript_alignments = parse_gff_file(gff_files) logger.info( - f"read {len(transcript_alignments)} transcript alignments from {gff_infile}" + f"read {len(transcript_alignments)} transcript alignments from file(s): {', '.join(gff_files)}" ) for transcript_exons in transcript_alignments.values(): diff --git a/tests/data/genomic_100.gff b/tests/data/genomic_100.gff deleted file mode 100644 index 5e69ecf..0000000 --- a/tests/data/genomic_100.gff +++ /dev/null @@ -1,100 +0,0 @@ -##gff-version 3 -#!gff-spec-version 1.21 -#!processor NCBI annotwriter -#!genome-build GRCh37.p13 -#!genome-build-accession NCBI_Assembly:GCF_000001405.25 -#!annotation-date 03/07/2022 -#!annotation-source NCBI Homo sapiens Updated Annotation Release 105.20220307 -##sequence-region NC_000001.10 1 249250621 -##species https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 -NC_000001.10 RefSeq region 1 249250621 . + . ID=NC_000001.10:1..249250621;Dbxref=taxon:9606;Name=1;chromosome=1;gbkey=Src;genome=chromosome;mol_type=genomic DNA -NC_000001.10 BestRefSeq pseudogene 11874 14409 . + . ID=gene-DDX11L1;Dbxref=GeneID:100287102,HGNC:HGNC:37102;Name=DDX11L1;description=DEAD/H-box helicase 11 like 1 (pseudogene);gbkey=Gene;gene=DDX11L1;gene_biotype=transcribed_pseudogene;pseudo=true -NC_000001.10 BestRefSeq transcript 11874 14409 . + . ID=rna-NR_046018.2;Parent=gene-DDX11L1;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;Name=NR_046018.2;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 -NC_000001.10 BestRefSeq exon 11874 12227 . + . ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 -NC_000001.10 BestRefSeq exon 12613 12721 . + . ID=exon-NR_046018.2-2;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 -NC_000001.10 BestRefSeq exon 13221 14409 . + . ID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;Dbxref=GeneID:100287102,Genbank:NR_046018.2,HGNC:HGNC:37102;gbkey=misc_RNA;gene=DDX11L1;product=DEAD/H-box helicase 11 like 1 (pseudogene);pseudo=true;transcript_id=NR_046018.2 -NC_000001.10 BestRefSeq pseudogene 14362 29370 . - . ID=gene-WASH7P;Dbxref=GeneID:653635,HGNC:HGNC:38034;Name=WASH7P;description=WASP family homolog 7%2C pseudogene;gbkey=Gene;gene=WASH7P;gene_biotype=transcribed_pseudogene;gene_synonym=FAM39F,WASH5P;pseudo=true -NC_000001.10 BestRefSeq transcript 14362 29370 . - . ID=rna-NR_024540.1;Parent=gene-WASH7P;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;Name=NR_024540.1;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 29321 29370 . - . ID=exon-NR_024540.1-1;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 24738 24891 . - . ID=exon-NR_024540.1-2;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 18268 18366 . - . ID=exon-NR_024540.1-3;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 17915 18061 . - . ID=exon-NR_024540.1-4;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 17606 17742 . - . ID=exon-NR_024540.1-5;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 17233 17368 . - . ID=exon-NR_024540.1-6;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 16858 17055 . - . ID=exon-NR_024540.1-7;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 16607 16765 . - . ID=exon-NR_024540.1-8;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 15796 15947 . - . ID=exon-NR_024540.1-9;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 14970 15038 . - . ID=exon-NR_024540.1-10;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq exon 14362 14829 . - . ID=exon-NR_024540.1-11;Parent=rna-NR_024540.1;Dbxref=GeneID:653635,Genbank:NR_024540.1,HGNC:HGNC:38034;gbkey=misc_RNA;gene=WASH7P;product=WASP family homolog 7%2C pseudogene;pseudo=true;transcript_id=NR_024540.1 -NC_000001.10 BestRefSeq gene 17369 17436 . - . ID=gene-MIR6859-1;Dbxref=GeneID:102466751,HGNC:HGNC:50039,miRBase:MI0022705;Name=MIR6859-1;description=microRNA 6859-1;gbkey=Gene;gene=MIR6859-1;gene_biotype=miRNA;gene_synonym=hsa-mir-6859-1 -NC_000001.10 BestRefSeq primary_transcript 17369 17436 . - . ID=rna-NR_106918.1;Parent=gene-MIR6859-1;Dbxref=GeneID:102466751,Genbank:NR_106918.1,HGNC:HGNC:50039,miRBase:MI0022705;Name=NR_106918.1;gbkey=precursor_RNA;gene=MIR6859-1;product=microRNA 6859-1;transcript_id=NR_106918.1 -NC_000001.10 BestRefSeq exon 17369 17436 . - . ID=exon-NR_106918.1-1;Parent=rna-NR_106918.1;Dbxref=GeneID:102466751,Genbank:NR_106918.1,HGNC:HGNC:50039,miRBase:MI0022705;gbkey=precursor_RNA;gene=MIR6859-1;product=microRNA 6859-1;transcript_id=NR_106918.1 -NC_000001.10 BestRefSeq miRNA 17369 17391 . - . ID=rna-MIR6859-1;Parent=rna-NR_106918.1;Dbxref=GeneID:102466751,miRBase:MIMAT0027619,HGNC:HGNC:50039,miRBase:MI0022705;gbkey=ncRNA;gene=MIR6859-1;product=hsa-miR-6859-3p -NC_000001.10 BestRefSeq exon 17369 17391 . - . ID=exon-MIR6859-1-1;Parent=rna-MIR6859-1;Dbxref=GeneID:102466751,miRBase:MIMAT0027619,HGNC:HGNC:50039,miRBase:MI0022705;gbkey=ncRNA;gene=MIR6859-1;product=hsa-miR-6859-3p -NC_000001.10 BestRefSeq miRNA 17409 17431 . - . ID=rna-MIR6859-1-2;Parent=rna-NR_106918.1;Dbxref=GeneID:102466751,miRBase:MIMAT0027618,HGNC:HGNC:50039,miRBase:MI0022705;gbkey=ncRNA;gene=MIR6859-1;product=hsa-miR-6859-5p -NC_000001.10 BestRefSeq exon 17409 17431 . - . ID=exon-MIR6859-1-2-1;Parent=rna-MIR6859-1-2;Dbxref=GeneID:102466751,miRBase:MIMAT0027618,HGNC:HGNC:50039,miRBase:MI0022705;gbkey=ncRNA;gene=MIR6859-1;product=hsa-miR-6859-5p -NC_000001.10 BestRefSeq gene 30366 30503 . + . ID=gene-MIR1302-2;Dbxref=GeneID:100302278,HGNC:HGNC:35294,miRBase:MI0006363;Name=MIR1302-2;description=microRNA 1302-2;gbkey=Gene;gene=MIR1302-2;gene_biotype=miRNA;gene_synonym=hsa-mir-1302-2,MIRN1302-2 -NC_000001.10 BestRefSeq primary_transcript 30366 30503 . + . ID=rna-NR_036051.1;Parent=gene-MIR1302-2;Dbxref=GeneID:100302278,Genbank:NR_036051.1,HGNC:HGNC:35294,miRBase:MI0006363;Name=NR_036051.1;gbkey=precursor_RNA;gene=MIR1302-2;product=microRNA 1302-2;transcript_id=NR_036051.1 -NC_000001.10 BestRefSeq exon 30366 30503 . + . ID=exon-NR_036051.1-1;Parent=rna-NR_036051.1;Dbxref=GeneID:100302278,Genbank:NR_036051.1,HGNC:HGNC:35294,miRBase:MI0006363;gbkey=precursor_RNA;gene=MIR1302-2;product=microRNA 1302-2;transcript_id=NR_036051.1 -NC_000001.10 BestRefSeq miRNA 30438 30458 . + . ID=rna-MIR1302-2;Parent=rna-NR_036051.1;Dbxref=GeneID:100302278,miRBase:MIMAT0005890,HGNC:HGNC:35294,miRBase:MI0006363;gbkey=ncRNA;gene=MIR1302-2;product=hsa-miR-1302 -NC_000001.10 BestRefSeq exon 30438 30458 . + . ID=exon-MIR1302-2-1;Parent=rna-MIR1302-2;Dbxref=GeneID:100302278,miRBase:MIMAT0005890,HGNC:HGNC:35294,miRBase:MI0006363;gbkey=ncRNA;gene=MIR1302-2;product=hsa-miR-1302 -NC_000001.10 BestRefSeq gene 34611 36081 . - . ID=gene-FAM138A;Dbxref=GeneID:645520,HGNC:HGNC:32334;Name=FAM138A;description=family with sequence similarity 138 member A;gbkey=Gene;gene=FAM138A;gene_biotype=lncRNA;gene_synonym=F379,FAM138F -NC_000001.10 BestRefSeq lnc_RNA 34611 36081 . - . ID=rna-NR_026818.1;Parent=gene-FAM138A;Dbxref=GeneID:645520,Genbank:NR_026818.1,HGNC:HGNC:32334;Name=NR_026818.1;gbkey=ncRNA;gene=FAM138A;product=family with sequence similarity 138 member A;transcript_id=NR_026818.1 -NC_000001.10 BestRefSeq exon 35721 36081 . - . ID=exon-NR_026818.1-1;Parent=rna-NR_026818.1;Dbxref=GeneID:645520,Genbank:NR_026818.1,HGNC:HGNC:32334;gbkey=ncRNA;gene=FAM138A;product=family with sequence similarity 138 member A;transcript_id=NR_026818.1 -NC_000001.10 BestRefSeq exon 35277 35481 . - . ID=exon-NR_026818.1-2;Parent=rna-NR_026818.1;Dbxref=GeneID:645520,Genbank:NR_026818.1,HGNC:HGNC:32334;gbkey=ncRNA;gene=FAM138A;product=family with sequence similarity 138 member A;transcript_id=NR_026818.1 -NC_000001.10 BestRefSeq exon 34611 35174 . - . ID=exon-NR_026818.1-3;Parent=rna-NR_026818.1;Dbxref=GeneID:645520,Genbank:NR_026818.1,HGNC:HGNC:32334;gbkey=ncRNA;gene=FAM138A;product=family with sequence similarity 138 member A;transcript_id=NR_026818.1 -NC_000001.10 Curated Genomic pseudogene 52453 53396 . + . ID=gene-OR4G4P;Dbxref=GeneID:79504,HGNC:HGNC:14822;Name=OR4G4P;description=olfactory receptor family 4 subfamily G member 4 pseudogene;gbkey=Gene;gene=OR4G4P;gene_biotype=pseudogene;pseudo=true -NC_000001.10 Curated Genomic pseudogene 63016 63885 . + . ID=gene-OR4G11P;Dbxref=GeneID:403263,HGNC:HGNC:31276;Name=OR4G11P;description=olfactory receptor family 4 subfamily G member 11 pseudogene;gbkey=Gene;gene=OR4G11P;gene_biotype=pseudogene;pseudo=true -NC_000001.10 BestRefSeq gene 65419 71585 . + . ID=gene-OR4F5;Dbxref=GeneID:79501,HGNC:HGNC:14825;Name=OR4F5;description=olfactory receptor family 4 subfamily F member 5;gbkey=Gene;gene=OR4F5;gene_biotype=protein_coding -NC_000001.10 BestRefSeq mRNA 65419 71585 . + . ID=rna-NM_001005484.2;Parent=gene-OR4F5;Dbxref=GeneID:79501,Genbank:NM_001005484.2,HGNC:HGNC:14825;Name=NM_001005484.2;gbkey=mRNA;gene=OR4F5;product=olfactory receptor family 4 subfamily F member 5;tag=RefSeq Select;transcript_id=NM_001005484.2 -NC_000001.10 BestRefSeq exon 65419 65433 . + . ID=exon-NM_001005484.2-1;Parent=rna-NM_001005484.2;Dbxref=GeneID:79501,Genbank:NM_001005484.2,HGNC:HGNC:14825;gbkey=mRNA;gene=OR4F5;product=olfactory receptor family 4 subfamily F member 5;tag=RefSeq Select;transcript_id=NM_001005484.2 -NC_000001.10 BestRefSeq exon 65520 65573 . + . ID=exon-NM_001005484.2-2;Parent=rna-NM_001005484.2;Dbxref=GeneID:79501,Genbank:NM_001005484.2,HGNC:HGNC:14825;gbkey=mRNA;gene=OR4F5;product=olfactory receptor family 4 subfamily F member 5;tag=RefSeq Select;transcript_id=NM_001005484.2 -NC_000001.10 BestRefSeq exon 69037 71585 . + . ID=exon-NM_001005484.2-3;Parent=rna-NM_001005484.2;Dbxref=GeneID:79501,Genbank:NM_001005484.2,HGNC:HGNC:14825;gbkey=mRNA;gene=OR4F5;product=olfactory receptor family 4 subfamily F member 5;tag=RefSeq Select;transcript_id=NM_001005484.2 -NC_000001.10 BestRefSeq CDS 65565 65573 . + 0 ID=cds-NP_001005484.2;Parent=rna-NM_001005484.2;Dbxref=CCDS:CCDS30547.1,GeneID:79501,Genbank:NP_001005484.2,HGNC:HGNC:14825;Name=NP_001005484.2;gbkey=CDS;gene=OR4F5;product=olfactory receptor 4F5;protein_id=NP_001005484.2;tag=RefSeq Select -NC_000001.10 BestRefSeq CDS 69037 70008 . + 0 ID=cds-NP_001005484.2;Parent=rna-NM_001005484.2;Dbxref=CCDS:CCDS30547.1,GeneID:79501,Genbank:NP_001005484.2,HGNC:HGNC:14825;Name=NP_001005484.2;gbkey=CDS;gene=OR4F5;product=olfactory receptor 4F5;protein_id=NP_001005484.2;tag=RefSeq Select -NC_000001.10 Curated Genomic pseudogene 126642 129225 . - . ID=gene-SEPTIN14P18;Dbxref=GeneID:107126284,HGNC:HGNC:51705;Name=SEPTIN14P18;description=septin 14 pseudogene 18;gbkey=Gene;gene=SEPTIN14P18;gene_biotype=pseudogene;gene_synonym=SEPT14P18;pseudo=true -NC_000001.10 Curated Genomic exon 126642 129225 . - . ID=id-SEPTIN14P18;Parent=gene-SEPTIN14P18;Dbxref=GeneID:107126284,HGNC:HGNC:51705;gbkey=exon;gene=SEPTIN14P18;pseudo=true -NC_000001.10 Curated Genomic pseudogene 131068 134836 . + . ID=gene-CICP27;Dbxref=GeneID:100420257,HGNC:HGNC:48835;Name=CICP27;description=capicua transcriptional repressor pseudogene 27;gbkey=Gene;gene=CICP27;gene_biotype=pseudogene;pseudo=true -NC_000001.10 BestRefSeq gene 134773 140566 . - . ID=gene-LOC729737;Dbxref=GeneID:729737;Name=LOC729737;description=uncharacterized LOC729737;gbkey=Gene;gene=LOC729737;gene_biotype=lncRNA -NC_000001.10 BestRefSeq lnc_RNA 134773 140566 . - . ID=rna-NR_039983.2;Parent=gene-LOC729737;Dbxref=GeneID:729737,Genbank:NR_039983.2;Name=NR_039983.2;gbkey=ncRNA;gene=LOC729737;product=uncharacterized LOC729737;transcript_id=NR_039983.2 -NC_000001.10 BestRefSeq exon 140075 140566 . - . ID=exon-NR_039983.2-1;Parent=rna-NR_039983.2;Dbxref=GeneID:729737,Genbank:NR_039983.2;gbkey=ncRNA;gene=LOC729737;product=uncharacterized LOC729737;transcript_id=NR_039983.2 -NC_000001.10 BestRefSeq exon 139790 139847 . - . ID=exon-NR_039983.2-2;Parent=rna-NR_039983.2;Dbxref=GeneID:729737,Genbank:NR_039983.2;gbkey=ncRNA;gene=LOC729737;product=uncharacterized LOC729737;transcript_id=NR_039983.2 -NC_000001.10 BestRefSeq exon 134773 139696 . - . ID=exon-NR_039983.2-3;Parent=rna-NR_039983.2;Dbxref=GeneID:729737,Genbank:NR_039983.2;gbkey=ncRNA;gene=LOC729737;product=uncharacterized LOC729737;transcript_id=NR_039983.2 -NC_000001.10 Curated Genomic pseudogene 157784 157887 . - . ID=gene-RNU6-1100P;Dbxref=GeneID:106480049,HGNC:HGNC:48063;Name=RNU6-1100P;description=RNA%2C U6 small nuclear 1100%2C pseudogene;gbkey=Gene;gene=RNU6-1100P;gene_biotype=pseudogene;pseudo=true -NC_000001.10 Curated Genomic pseudogene 164689 164792 . + . ID=gene-GTF2IP10;Dbxref=GeneID:107403151,HGNC:HGNC:51722;Name=GTF2IP10;description=general transcription factor IIi pseudogene 10;gbkey=Gene;gene=GTF2IP10;gene_biotype=pseudogene;pseudo=true -NC_000001.10 Curated Genomic pseudogene 228262 228787 . - . ID=gene-RPL23AP21;Dbxref=GeneID:728481,HGNC:HGNC:35827;Name=RPL23AP21;description=ribosomal protein L23a pseudogene 21;gbkey=Gene;gene=RPL23AP21;gene_biotype=pseudogene;gene_synonym=RPL23A_1_1;pseudo=true -NC_000001.10 Curated Genomic exon 228262 228787 . - . ID=id-RPL23AP21;Parent=gene-RPL23AP21;Dbxref=GeneID:728481,HGNC:HGNC:35827;gbkey=exon;gene=RPL23AP21;pseudo=true -NC_000001.10 BestRefSeq gene 323892 328581 . + . ID=gene-LOC100132287;Dbxref=GeneID:100132287;Name=LOC100132287;description=uncharacterized LOC100132287;gbkey=Gene;gene=LOC100132287;gene_biotype=lncRNA -NC_000001.10 BestRefSeq lnc_RNA 323892 328581 . + . ID=rna-NR_028322.1;Parent=gene-LOC100132287;Dbxref=GeneID:100132287,Genbank:NR_028322.1;Name=NR_028322.1;gbkey=ncRNA;gene=LOC100132287;product=uncharacterized LOC100132287;transcript_id=NR_028322.1 -NC_000001.10 BestRefSeq exon 323892 324060 . + . ID=exon-NR_028322.1-1;Parent=rna-NR_028322.1;Dbxref=GeneID:100132287,Genbank:NR_028322.1;gbkey=ncRNA;gene=LOC100132287;product=uncharacterized LOC100132287;transcript_id=NR_028322.1 -NC_000001.10 BestRefSeq exon 324288 324345 . + . ID=exon-NR_028322.1-2;Parent=rna-NR_028322.1;Dbxref=GeneID:100132287,Genbank:NR_028322.1;gbkey=ncRNA;gene=LOC100132287;product=uncharacterized LOC100132287;transcript_id=NR_028322.1 -NC_000001.10 BestRefSeq exon 324439 328581 . + . ID=exon-NR_028322.1-3;Parent=rna-NR_028322.1;Dbxref=GeneID:100132287,Genbank:NR_028322.1;gbkey=ncRNA;gene=LOC100132287;product=uncharacterized LOC100132287;transcript_id=NR_028322.1 -NC_000001.10 Curated Genomic pseudogene 328518 332282 . - . ID=gene-CICP7;Dbxref=GeneID:100288667,HGNC:HGNC:37756;Name=CICP7;description=capicua transcriptional repressor pseudogene 7;gbkey=Gene;gene=CICP7;gene_biotype=pseudogene;pseudo=true -NC_000001.10 Curated Genomic pseudogene 334127 334504 . + . ID=gene-SEPTIN14P13;Dbxref=GeneID:106481733,HGNC:HGNC:51700;Name=SEPTIN14P13;description=septin 14 pseudogene 13;gbkey=Gene;gene=SEPTIN14P13;gene_biotype=pseudogene;gene_synonym=SEPT14P13;pseudo=true -NC_000001.10 Curated Genomic exon 334127 334504 . + . ID=id-SEPTIN14P13;Parent=gene-SEPTIN14P13;Dbxref=GeneID:106481733,HGNC:HGNC:51700;gbkey=exon;gene=SEPTIN14P13;pseudo=true -NC_000001.10 BestRefSeq gene 367659 368597 . + . ID=gene-OR4F29;Dbxref=GeneID:729759,HGNC:HGNC:31275;Name=OR4F29;description=olfactory receptor family 4 subfamily F member 29;gbkey=Gene;gene=OR4F29;gene_biotype=protein_coding;gene_synonym=OR7-21 -NC_000001.10 BestRefSeq mRNA 367659 368597 . + . ID=rna-NM_001005221.2;Parent=gene-OR4F29;Dbxref=GeneID:729759,Genbank:NM_001005221.2,HGNC:HGNC:31275;Name=NM_001005221.2;gbkey=mRNA;gene=OR4F29;product=olfactory receptor family 4 subfamily F member 29;tag=RefSeq Select;transcript_id=NM_001005221.2 -NC_000001.10 BestRefSeq exon 367659 368597 . + . ID=exon-NM_001005221.2-1;Parent=rna-NM_001005221.2;Dbxref=GeneID:729759,Genbank:NM_001005221.2,HGNC:HGNC:31275;gbkey=mRNA;gene=OR4F29;product=olfactory receptor family 4 subfamily F member 29;tag=RefSeq Select;transcript_id=NM_001005221.2 -NC_000001.10 BestRefSeq CDS 367659 368597 . + 0 ID=cds-NP_001005221.2;Parent=rna-NM_001005221.2;Dbxref=CCDS:CCDS72675.1,GeneID:729759,Genbank:NP_001005221.2,HGNC:HGNC:31275;Name=NP_001005221.2;gbkey=CDS;gene=OR4F29;product=olfactory receptor 4F3/4F16/4F29;protein_id=NP_001005221.2;tag=RefSeq Select -NC_000001.10 Curated Genomic pseudogene 379067 379573 . - . ID=gene-WBP1LP7;Dbxref=GeneID:106479030,HGNC:HGNC:43955;Name=WBP1LP7;description=WBP1L pseudogene 7;gbkey=Gene;gene=WBP1LP7;gene_biotype=pseudogene;pseudo=true -NC_000001.10 Curated Genomic exon 379067 379573 . - . ID=id-WBP1LP7;Parent=gene-WBP1LP7;Dbxref=GeneID:106479030,HGNC:HGNC:43955;gbkey=exon;gene=WBP1LP7;pseudo=true -NC_000001.10 Curated Genomic pseudogene 470896 471368 . + . ID=gene-RPL23AP24;Dbxref=GeneID:728517,HGNC:HGNC:36176;Name=RPL23AP24;description=ribosomal protein L23a pseudogene 24;gbkey=Gene;gene=RPL23AP24;gene_biotype=pseudogene;gene_synonym=RPL23A_1_2;pseudo=true -NC_000001.10 Curated Genomic exon 470896 471368 . + . ID=id-RPL23AP24;Parent=gene-RPL23AP24;Dbxref=GeneID:728517,HGNC:HGNC:36176;gbkey=exon;gene=RPL23AP24;pseudo=true -NC_000001.10 BestRefSeq gene 562760 564389 . - . ID=gene-LOC101928626;Dbxref=GeneID:101928626;Name=LOC101928626;description=uncharacterized LOC101928626;gbkey=Gene;gene=LOC101928626;gene_biotype=lncRNA -NC_000001.10 BestRefSeq lnc_RNA 562760 564389 . - . ID=rna-NR_125957.1;Parent=gene-LOC101928626;Dbxref=GeneID:101928626,Genbank:NR_125957.1;Name=NR_125957.1;gbkey=ncRNA;gene=LOC101928626;product=uncharacterized LOC101928626;transcript_id=NR_125957.1 -NC_000001.10 BestRefSeq exon 564299 564389 . - . ID=exon-NR_125957.1-1;Parent=rna-NR_125957.1;Dbxref=GeneID:101928626,Genbank:NR_125957.1;gbkey=ncRNA;gene=LOC101928626;product=uncharacterized LOC101928626;transcript_id=NR_125957.1 -NC_000001.10 BestRefSeq exon 563341 563603 . - . ID=exon-NR_125957.1-2;Parent=rna-NR_125957.1;Dbxref=GeneID:101928626,Genbank:NR_125957.1;gbkey=ncRNA;gene=LOC101928626;product=uncharacterized LOC101928626;transcript_id=NR_125957.1 -NC_000001.10 BestRefSeq exon 562760 563203 . - . ID=exon-NR_125957.1-3;Parent=rna-NR_125957.1;Dbxref=GeneID:101928626,Genbank:NR_125957.1;gbkey=ncRNA;gene=LOC101928626;product=uncharacterized LOC101928626;transcript_id=NR_125957.1 -NC_000001.10 Curated Genomic pseudogene 564442 564820 . + . ID=gene-MTND1P23;Dbxref=GeneID:100887749,HGNC:HGNC:42092;Name=MTND1P23;description=MT-ND1 pseudogene 23;gbkey=Gene;gene=MTND1P23;gene_biotype=pseudogene;pseudo=true -NC_000001.10 Curated Genomic pseudogene 565020 566066 . + . ID=gene-MTND2P28;Dbxref=GeneID:100652939,HGNC:HGNC:42129;Name=MTND2P28;description=MT-ND2 pseudogene 28;gbkey=Gene;gene=MTND2P28;gene_biotype=pseudogene;pseudo=true -NC_000001.10 Curated Genomic pseudogene 566454 567996 . + . ID=gene-MTCO1P12;Dbxref=GeneID:107075141,HGNC:HGNC:52014;Name=MTCO1P12;description=MT-CO1 pseudogene 12;gbkey=Gene;gene=MTCO1P12;gene_biotype=pseudogene;pseudo=true -NC_000001.10 RefSeqFE silencer 567489 567783 . . . ID=id-GeneID:121967041;Dbxref=GeneID:121967041;Note=tiled region #5852%3B K562 Repressive DNase matched - State 24:Quies;experiment=EXISTENCE:reporter gene assay evidence [ECO:0000049][PMID:27701403];function=represses a minimal TATA promoter and a strong SV40 promoter by Sharpr-MPRA in K562 cells {active_cell/tissue: K562};gbkey=regulatory;regulatory_class=silencer -NC_000001.10 RefSeqFE biological_region 567489 567783 . + . ID=id-GeneID:121967041-2;Dbxref=GeneID:121967041;Name=biological region;gbkey=Region;standard_name=Sharpr-MPRA regulatory region 5852 -NC_000001.10 BestRefSeq gene 567995 568065 . - . ID=gene-MIR12136;Dbxref=GeneID:113219467,HGNC:HGNC:54043,miRBase:MI0039740;Name=MIR12136;description=microRNA 12136;gbkey=Gene;gene=MIR12136;gene_biotype=miRNA -NC_000001.10 BestRefSeq primary_transcript 567995 568065 . - . ID=rna-NR_162149.1;Parent=gene-MIR12136;Dbxref=GeneID:113219467,Genbank:NR_162149.1,HGNC:HGNC:54043,miRBase:MI0039740;Name=NR_162149.1;gbkey=precursor_RNA;gene=MIR12136;product=microRNA 12136;transcript_id=NR_162149.1 -NC_000001.10 BestRefSeq exon 567995 568065 . - . ID=exon-NR_162149.1-1;Parent=rna-NR_162149.1;Dbxref=GeneID:113219467,Genbank:NR_162149.1,HGNC:HGNC:54043,miRBase:MI0039740;gbkey=precursor_RNA;gene=MIR12136;product=microRNA 12136;transcript_id=NR_162149.1 -NC_000001.10 BestRefSeq miRNA 568048 568065 . - . ID=rna-MIR12136;Parent=rna-NR_162149.1;Dbxref=GeneID:113219467,miRBase:MIMAT0049032,HGNC:HGNC:54043,miRBase:MI0039740;gbkey=ncRNA;gene=MIR12136;product=hsa-miR-12136 -NC_000001.10 BestRefSeq exon 568048 568065 . - . ID=exon-MIR12136-1;Parent=rna-MIR12136;Dbxref=GeneID:113219467,miRBase:MIMAT0049032,HGNC:HGNC:54043,miRBase:MI0039740;gbkey=ncRNA;gene=MIR12136;product=hsa-miR-12136 -NC_000001.10 Curated Genomic pseudogene 568137 568818 . + . ID=gene-MTCO2P12;Dbxref=GeneID:107075310,HGNC:HGNC:52028;Name=MTCO2P12;description=MT-CO2 pseudogene 12;gbkey=Gene;gene=MTCO2P12;gene_biotype=pseudogene;gene_synonym=COII,COX2,COXII,MT-CO2,MTCO2;pseudo=true -NC_000001.10 Curated Genomic pseudogene 568915 569121 . + . ID=gene-MTATP8P1;Dbxref=GeneID:106480795,HGNC:HGNC:44571;Name=MTATP8P1;description=MT-ATP8 pseudogene 1;gbkey=Gene;gene=MTATP8P1;gene_biotype=pseudogene;pseudo=true -NC_000001.10 Curated Genomic pseudogene 569076 569756 . + . 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z;pHP?9%E6LGzj>PW^*xx2D8T69cPHq#>EdBNQUa`V3{2EZLsW<>oQpWAu{iRZF9-1 z1zu^i1i>tDT=VKgty{7}z@|*-b#`8J${Jwba>|lzlUImbXW5-pRF=IEV*6=jgP)d< z-w}R($4dcczH=y4Rc?vgxDCZ1s3jXiH*P~UAXk^6RO-5Jb;7Eyc_|6@Ew9c2{xx8A zns*;lsa`%dBY9G Date: Mon, 11 Mar 2024 15:09:24 -0700 Subject: [PATCH 13/54] style(IPVC-2262): black --- sbin/ncbi_parse_genomic_gff.py | 4 +--- tests/test_ncbi_parse_genomic_gff.py | 18 ++++++++++++------ 2 files changed, 13 insertions(+), 9 deletions(-) diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 9f1f741..11ef62d 100644 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -144,9 +144,7 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: logger = logging.getLogger(__name__) parser = argparse.ArgumentParser(description="Parse GFF file.") - parser.add_argument( - "gff_files", nargs='+', type=str, help="Path to GFF file(s)" - ) + parser.add_argument("gff_files", nargs="+", type=str, help="Path to GFF file(s)") args = parser.parse_args() gff_files = args.gff_files diff --git a/tests/test_ncbi_parse_genomic_gff.py b/tests/test_ncbi_parse_genomic_gff.py index 90a20e2..c3ae479 100644 --- a/tests/test_ncbi_parse_genomic_gff.py +++ b/tests/test_ncbi_parse_genomic_gff.py @@ -165,13 +165,13 @@ def test_parse_gff_file(self): def test_parse_gff_file_accepts_gzipped_files(self): # Create a gzipped version of the temp_gff file - with gzip.open(self.temp_gff.name + '.gz', 'wb') as f_out: - with open(self.temp_gff.name, 'rb') as f_in: + with gzip.open(self.temp_gff.name + ".gz", "wb") as f_out: + with open(self.temp_gff.name, "rb") as f_in: f_out.write(f_in.read()) # Test parsing the gzipped GFF file expected_result = {"rna-NR_046018.2": self.gff_records} - parsed_result = parse_gff_file([self.temp_gff.name + '.gz']) + parsed_result = parse_gff_file([self.temp_gff.name + ".gz"]) self.assertEqual(parsed_result, expected_result) def test_get_zero_based_exon_ranges(self): @@ -185,13 +185,19 @@ def test_script_output(self): script_path = os.path.join(BASE_DIR, "sbin", "ncbi_parse_genomic_gff.py") command = ["python", script_path, input_gff_file] - completed_process = subprocess.run(command, check=True, capture_output=True, text=True) + completed_process = subprocess.run( + command, check=True, capture_output=True, text=True + ) stdout_content = completed_process.stdout - expected_file_path = os.path.join(CURRENT_DIR, "data", "expected_genomic_100.exonset") + expected_file_path = os.path.join( + CURRENT_DIR, "data", "expected_genomic_100.exonset" + ) with open(expected_file_path, "r") as expected_file: expected_content = expected_file.read() - assert stdout_content == expected_content, "Output content doesn't match expected." + assert ( + stdout_content == expected_content + ), "Output content doesn't match expected." if __name__ == "__main__": From 4945c4c1e296a7ad67264460ee14752c8f919445 Mon Sep 17 00:00:00 2001 From: Paige Taylor Date: Mon, 11 Mar 2024 15:43:24 -0700 Subject: [PATCH 14/54] style(IPVC-2262): optimize imports --- tests/test_ncbi_parse_genomic_gff.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/test_ncbi_parse_genomic_gff.py b/tests/test_ncbi_parse_genomic_gff.py index c3ae479..71d92f9 100644 --- a/tests/test_ncbi_parse_genomic_gff.py +++ b/tests/test_ncbi_parse_genomic_gff.py @@ -1,14 +1,14 @@ import gzip +import os import subprocess import unittest from tempfile import NamedTemporaryFile -import os from sbin.ncbi_parse_genomic_gff import ( + get_zero_based_exon_ranges, GFFRecord, parse_gff_file, parse_gff_record, - get_zero_based_exon_ranges, ) CURRENT_DIR = os.path.dirname(os.path.abspath(__file__)) From 5aca4b6e783027450f70e63bf707d4d006a2f9c5 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Wed, 13 Mar 2024 20:35:44 -0600 Subject: [PATCH 15/54] feat(IPVC-2246): add seqrepo load to uta build script (#6) --- README.md | 51 +++++++++++++++++++++++++++++++----- etc/global.conf | 2 +- etc/scripts/run-uta-build.sh | 31 +++++++++++++++------- sbin/uta-diff | 2 +- 4 files changed, 68 insertions(+), 18 deletions(-) diff --git a/README.md b/README.md index d286edb..bde8eea 100644 --- a/README.md +++ b/README.md @@ -294,22 +294,59 @@ To run local build of UTA you can follow these steps... 1. Pick a UTA version as the base (ex. "uta_20210129b") -2. Setup local database follow the steps above (`Installing with Docker`) to pull image, - start and populate. __NOTE__...it does take about 5 minutes for the local database is ready. +2. Setup local UTA Postgresql database Docker container by following the steps above + (`Installing with Docker`). __NOTE__...It takes about 5 minutes before the local + database is ready. -3. Build the image +3. Setup local SeqRepo Docker volume. This can be done by running the following two + commands. __NOTE__...this step does require around ~13Gb of local disk space and + takes roughly 30 minutes to complete. You need to wait until the `Docker run` + command completes before moving to the next step. - $ docker build -t uta . + $ docker pull biocommons/seqrepo + $ docker run --name seqrepo biocommons/seqrepo + +4. Build the image -4. Run UTA build. Supplying a local path with data needed for the workflow is a requirement. + $ docker build -t uta-build . + +5. Run UTA build. Supplying a local path with data needed for the workflow is a requirement. $ docker run \ -it \ --rm \ --name uta-build \ --volume .:/opt/repos/uta \ - --volume /System/Volumes/Data/locus/data/test-uta-22:/temp \ + --volume :/temp \ + --volumes-from seqrepo \ --network=host uta-build:latest -5. Once in the container you can run this script to create new schema, process input files, +6. Once in the container you can run this script to create new schema, process input files, update the database, and dump a new artifact. + + $ bash etc/scripts/run-uta-build.sh + + This update script is dependent on the following directory structure. + + $ tree . + . + ├── gene + │ └── DATA + │ ├── GENE_INFO + │ │ └── Mammalia + │ │ └── Homo_sapiens.gene_info.gz + │ └── gene2accession.gz + ├── genomes + │ └── refseq + │ └── vertebrate_mammalian + │ └── Homo_sapiens + │ └── all_assembly_versions + │ └── GCF_000001405.25_GRCh37.p13 + │ ├── GCF_000001405.25_GRCh37.p13_genomic.fna.gz + │ └── GCF_000001405.25_GRCh37.p13_genomic.gff.gz + └── refseq + └── H_sapiens + └── mRNA_Prot + ├── human.1.protein.faa.gz + ├── human.1.rna.fna.gz + └── human.1.rna.gbff.gz diff --git a/etc/global.conf b/etc/global.conf index c929b28..1aece93 100644 --- a/etc/global.conf +++ b/etc/global.conf @@ -16,7 +16,7 @@ aligner = utaaa fasta_directories = aux/sequences2 aux/sequences -seqrepo = /usr/local/share/seqrepo/latest +seqrepo = /usr/local/share/seqrepo/2024-02-20 #data/manual #data/bic/sequences.fasta.bgz diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh index 9052541..10458c7 100644 --- a/etc/scripts/run-uta-build.sh +++ b/etc/scripts/run-uta-build.sh @@ -1,17 +1,18 @@ #!/bin/bash -if [ "$#" -lt 1 ] +if [ "$#" -lt 2 ] then - echo "error: too few arguments, you provided $#, 1 required" - echo "usage: run_uta_build_gene.sh " + echo "error : too few arguments, you provided $#, 2 required" + echo "usage: run_uta_build_gene.sh " exit 1 fi source_uta_v=$1 +seqrepo_data_release=$2 set -euxo pipefail # set local variables and create working directories -loading_schema="uta_1_1" +loading_uta_v="uta_1_1" loading_dir="/temp/loading" dumps_dir="/temp/dumps" logs_dir="/temp/logs" @@ -20,8 +21,15 @@ for d in "$loading_dir" "$dumps_dir" "$logs_dir"; done ## Drop loading schema, and recreate -etc/scripts/delete-schema.sh $loading_schema -etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_schema" +etc/scripts/delete-schema.sh "$loading_uta_v" +etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_uta_v" + +## Load SeqRepo with new sequences +seqrepo load -n NCBI -i "$seqrepo_data_release" \ + /temp/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz \ + /temp/refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz \ + /temp/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.fna.gz 2>& 1 | \ + tee "$logs_dir/seqrepo-load.log" ### extract meta data # genes @@ -44,8 +52,13 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$loa tee "$logs_dir/load-geneinfo.log" # transcript info -#uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$loading_dir/gbff.txinfo.gz" 2>&1 | \ -# tee "$logs_dir/load-txinfo.log" +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$loading_dir/gbff.txinfo.gz" 2>&1 | \ + tee "$logs_dir/load-txinfo.log" + + +### run diff +sbin/uta-diff "$source_uta_v" "$loading_uta_v" + ### psql_dump -pg_dump -U uta_admin -h localhost -d uta -t "$loading_schema.gene" | gzip -c > "$dumps_dir/uta.pgd.gz" +pg_dump -U uta_admin -h localhost -d uta -t "$loading_uta_v.gene" | gzip -c > "$dumps_dir/uta.pgd.gz" diff --git a/sbin/uta-diff b/sbin/uta-diff index 489562d..4caa50e 100755 --- a/sbin/uta-diff +++ b/sbin/uta-diff @@ -44,7 +44,7 @@ if __name__ == "__main__": logging.basicConfig(level=logging.DEBUG) logger = logging.getLogger() - url = "postgresql://uta_admin@localhost/uta_dev" + url = "postgresql://uta_admin@localhost/uta" tables = ["associated_accessions", "exon", "exon_aln", "exon_set", "gene", "meta", "origin", "seq", "seq_anno", "transcript",] From 4a0e90eebfdec24585e52c0b1e170c965f27a399 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Thu, 14 Mar 2024 16:32:18 -0600 Subject: [PATCH 16/54] feat(IPVC-2268): added missing uta build steps (#8) --- etc/scripts/run-uta-build.sh | 16 ++++++++++++++++ sbin/exonset-to-seqinfo | 6 +++--- sbin/ncbi_parse_genomic_gff.py | 2 ++ src/uta/loading.py | 8 ++++---- 4 files changed, 25 insertions(+), 7 deletions(-) diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh index 10458c7..697b57f 100644 --- a/etc/scripts/run-uta-build.sh +++ b/etc/scripts/run-uta-build.sh @@ -45,6 +45,15 @@ GBFF_files=$(ls /temp/refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz) sbin/ncbi-parse-gbff "$GBFF_files" | gzip -c > "$loading_dir/gbff.txinfo.gz" 2>&1 | \ tee "$logs_dir/ncbi-parse-gbff.log" +# parse alignments from GFF input files +GFF_files=$(ls /temp/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.gff.gz) +sbin/ncbi_parse_genomic_gff.py "$GFF_files" | gzip -c > "$loading_dir/gff.exonsets.gz" 2>&1 | \ + tee "$logs_dir/ncbi-parse-genomic-gff.log" + +# generate seqinfo files from exonsets +sbin/exonset-to-seqinfo -o NCBI "$loading_dir/gff.exonsets.gz" | gzip -c > "$loading_dir/seqinfo.gz" 2>&1 | \ + tee "$logs_dir/exonset-to-seqinfo.log" + ### update the uta database # genes @@ -55,6 +64,13 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$loa uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$loading_dir/gbff.txinfo.gz" 2>&1 | \ tee "$logs_dir/load-txinfo.log" +# gff exon sets +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$loading_dir/gff.exonsets.gz" 2>&1 | \ + tee "$logs_dir/load-exonsets.log" + +# align exons +#uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | tee "$logs_dir/align-exons.log" + ### run diff sbin/uta-diff "$source_uta_v" "$loading_uta_v" diff --git a/sbin/exonset-to-seqinfo b/sbin/exonset-to-seqinfo index 1ccbf95..c6c711a 100755 --- a/sbin/exonset-to-seqinfo +++ b/sbin/exonset-to-seqinfo @@ -32,7 +32,7 @@ def parse_args(argv): required=True) ap.add_argument("--conf", default=[ - pkg_resources.resource_filename("uta", "../etc/global.conf")] + pkg_resources.resource_filename("uta", "../../etc/global.conf")] ) opts = ap.parse_args(argv) @@ -50,9 +50,9 @@ if __name__ == "__main__": opts = parse_args(sys.argv[1:]) - cf = ConfigParser.SafeConfigParser() + cf = ConfigParser.ConfigParser() for conf_fn in opts.conf: - cf.readfp(open(conf_fn)) + cf.read_file(open(conf_fn)) logger.info("loaded " + conf_fn) in_fn = opts.FILES[0] diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 11ef62d..8fb1169 100644 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -1,3 +1,5 @@ +#!/usr/bin/env python + """Write exonsets from NCBI GFF alignments, as obtained from ftp://ftp.ncbi.nlm.nih.gov/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions This service appeared in April 2015 and is due to update weekly. diff --git a/src/uta/loading.py b/src/uta/loading.py index 57ca5fa..e04a97a 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -42,13 +42,13 @@ def align_exons(session, opts, cf): def _get_cursor(con): cur = con.cursor(cursor_factory=psycopg2.extras.NamedTupleCursor) - cur.execute(text("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role")))) - cur.execute(text("set search_path = " + usam.schema_name)) + cur.execute("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role"))) + cur.execute("set search_path = " + usam.schema_name) return cur def align(s1, s2): - score, cigar = utaa.algorithms.needleman_wunsch_gotoh_align(s1.encode("ascii"), + score, cigar = utaa.align.algorithms.needleman_wunsch_gotoh_align(s1.encode("ascii"), s2.encode("ascii"), extended_cigar=True) tx_aseq, alt_aseq = utaa.algorithms.cigar_alignment( From 35215f95fca4bc608f7674349b0f396ca4ef2d4e Mon Sep 17 00:00:00 2001 From: nvta1209 <162694616+nvta1209@users.noreply.github.com> Date: Thu, 14 Mar 2024 20:13:25 -0700 Subject: [PATCH 17/54] feat(IPVC-2247): workflow skeleton (#5) --- .gitignore | 1 + Dockerfile | 10 +++------- README.md | 26 ++++++++++++++++---------- etc/scripts/create-new-schema.sh | 2 +- etc/scripts/delete-schema.sh | 2 +- etc/scripts/run-uta-build.sh | 0 misc/docker/load-uta.sh | 12 ++++++------ update/Dockerfile.download | 9 +++++++++ update/docker-compose.uta.yml | 30 ++++++++++++++++++++++++++++++ update/download.sh | 21 +++++++++++++++++++++ update/update.sh | 32 ++++++++++++++++++++++++++++++++ 11 files changed, 120 insertions(+), 25 deletions(-) mode change 100644 => 100755 etc/scripts/run-uta-build.sh create mode 100644 update/Dockerfile.download create mode 100644 update/docker-compose.uta.yml create mode 100755 update/download.sh create mode 100755 update/update.sh diff --git a/.gitignore b/.gitignore index d7fefb9..b0a2906 100644 --- a/.gitignore +++ b/.gitignore @@ -57,6 +57,7 @@ misc/ncbi-geneinfo/ncbi.geneinfo.tsv.gz misc/partial-alignments sdist tmp +update/ncbi/ venv nosetests.xml .vscode diff --git a/Dockerfile b/Dockerfile index 6718976..c65c41d 100644 --- a/Dockerfile +++ b/Dockerfile @@ -14,11 +14,7 @@ RUN python -m pip install --upgrade pip RUN pip install --upgrade setuptools RUN pip install pysam -ENV APP_HOME="/opt/repos/uta/" - -RUN mkdir -p ${APP_HOME} - -COPY . ${APP_HOME} -WORKDIR ${APP_HOME} - +WORKDIR /opt/repos/uta/ +COPY pyproject.toml . RUN pip install -e .[dev] +COPY . . diff --git a/README.md b/README.md index bde8eea..492929e 100644 --- a/README.md +++ b/README.md @@ -203,8 +203,8 @@ you will not need to install PostgreSQL or any of its dependencies. (code) version used to build the instance. $ psql -h localhost -U anonymous -d uta -c "select * from $uta_v.meta" - - key | value + + key | value ----------------+-------------------------------------------------------------------- schema_version | 1.1 created on | 2015-08-21T10:53:50.666152 @@ -213,7 +213,7 @@ you will not need to install PostgreSQL or any of its dependencies. (4 rows) 6. (Optional) To configure [hgvs](https://github.com/biocommons/hgvs) - to use this local installation, consult the + to use this local installation, consult the [hgvs documentation](https://hgvs.readthedocs.io/en/latest/installation.html#local-installation-of-uta-optional) ### Installing from database dumps @@ -243,7 +243,7 @@ the installation environment.* uta_admin are likely to ease installation. $ createuser -U postgres uta_admin - $ createdb -U postgres -O uta_admin uta + $ createdb -U postgres -O uta_admin uta 3. Restore the database. @@ -275,7 +275,7 @@ To develop UTA, follow these steps. ### Docker 1. Clone UTA and build docker image: - + $ git clone git@github.com:biocommons/uta.git $ cd uta $ docker build -t uta . @@ -294,13 +294,13 @@ To run local build of UTA you can follow these steps... 1. Pick a UTA version as the base (ex. "uta_20210129b") -2. Setup local UTA Postgresql database Docker container by following the steps above - (`Installing with Docker`). __NOTE__...It takes about 5 minutes before the local +2. Setup local UTA Postgresql database Docker container by following the steps above + (`Installing with Docker`). __NOTE__...It takes about 5 minutes before the local database is ready. 3. Setup local SeqRepo Docker volume. This can be done by running the following two - commands. __NOTE__...this step does require around ~13Gb of local disk space and - takes roughly 30 minutes to complete. You need to wait until the `Docker run` + commands. __NOTE__...this step does require around ~13Gb of local disk space and + takes roughly 30 minutes to complete. You need to wait until the `Docker run` command completes before moving to the next step. $ docker pull biocommons/seqrepo @@ -321,7 +321,7 @@ To run local build of UTA you can follow these steps... --volumes-from seqrepo \ --network=host uta-build:latest -6. Once in the container you can run this script to create new schema, process input files, +6. Once in the container you can run this script to create new schema, process input files, update the database, and dump a new artifact. $ bash etc/scripts/run-uta-build.sh @@ -350,3 +350,9 @@ To run local build of UTA you can follow these steps... ├── human.1.protein.faa.gz ├── human.1.rna.fna.gz └── human.1.rna.gbff.gz + +## Updating UTA + +To update UTA and SeqRepo, run `update.sh` from the `update` directory. + +The only dependencies of the script are bash and docker. diff --git a/etc/scripts/create-new-schema.sh b/etc/scripts/create-new-schema.sh index d13100a..0b6aa2b 100755 --- a/etc/scripts/create-new-schema.sh +++ b/etc/scripts/create-new-schema.sh @@ -21,4 +21,4 @@ pg_dump -U uta_admin -h localhost -d uta -n "$source_uta_v" | \ # create new schema gzip -cdq $dumps_dir/"$source_uta_v".pgd.gz | \ sbin/pg-dump-schema-rename "$source_uta_v" "$dest_uta_v" | \ - psql -U uta_admin -h localhost -d uta -aeE + psql -U uta_admin -h localhost -d uta -aeE \ No newline at end of file diff --git a/etc/scripts/delete-schema.sh b/etc/scripts/delete-schema.sh index a318799..e64eb3b 100755 --- a/etc/scripts/delete-schema.sh +++ b/etc/scripts/delete-schema.sh @@ -11,4 +11,4 @@ set -euxo pipefail source_uta_v=$1 -psql -h localhost -U uta_admin -d uta -c "DROP SCHEMA IF EXISTS $source_uta_v CASCADE" +psql -h localhost -U uta_admin -d uta -c "DROP SCHEMA IF EXISTS $source_uta_v CASCADE" \ No newline at end of file diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh old mode 100644 new mode 100755 diff --git a/misc/docker/load-uta.sh b/misc/docker/load-uta.sh index 3c9105b..e83ab20 100755 --- a/misc/docker/load-uta.sh +++ b/misc/docker/load-uta.sh @@ -9,7 +9,7 @@ # Overwrite pg_hba.conf, including whatever edits might have been made # by the postgres image -cat <"$PGDATA/pg_hba.conf" +cat <"$PGDATA/pg_hba.conf" # allow the anonymous user to access uta without password # These lines must occur before more stringent authentication methods host all anonymous 0.0.0.0/0 trust @@ -58,16 +58,16 @@ gzip -cdq < "${UTA_PGD_FN}" \ cat < -U anonymous -d uta -== +== == No password is required. -== +== ======================================================================= ======================================================================= diff --git a/update/Dockerfile.download b/update/Dockerfile.download new file mode 100644 index 0000000..e2481e4 --- /dev/null +++ b/update/Dockerfile.download @@ -0,0 +1,9 @@ +FROM debian:stable-slim + +RUN apt-get -y update +RUN apt-get -y install rsync + +WORKDIR /download_ncbi +VOLUME [ "/data/ncbi" ] +COPY download.sh ./ +CMD ./download.sh /data/ncbi diff --git a/update/docker-compose.uta.yml b/update/docker-compose.uta.yml new file mode 100644 index 0000000..6018725 --- /dev/null +++ b/update/docker-compose.uta.yml @@ -0,0 +1,30 @@ +version: '3' + +services: + seqrepo: + container_name: seqrepo + image: ubuntu:latest + command: echo 'Start SeqRepo, which is required for UTA update' + network_mode: host + uta: + container_name: uta + image: uta:${previous_uta_version} + environment: + - POSTGRES_HOST_AUTH_METHOD=trust + healthcheck: + test: pg_isready && psql -h localhost -U anonymous -d uta -c "select * from ${previous_uta_version}.meta" + interval: 10s + retries: 60 + network_mode: host + uta-update: + container_name: uta-update + image: uta-update:latest + command: etc/scripts/run-uta-build.sh ${previous_uta_version} + depends_on: + previous-uta: + condition: service_healthy + # seqrepo: + # condition: service_healthy + volumes: + - ./ncbi:/temp + network_mode: host diff --git a/update/download.sh b/update/download.sh new file mode 100755 index 0000000..ef3a0bf --- /dev/null +++ b/update/download.sh @@ -0,0 +1,21 @@ +#!/bin/bash + +set -e + +DOWNLOAD_DIR=$1 +DOWNLOAD_PATHS=( + 'gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz' + 'gene/DATA/gene2accession.gz' + 'refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz' +) + +for DOWNLOAD_PATH in "${DOWNLOAD_PATHS[@]}" +do + # each top-level directory in NCBI is an rsync module. + # bash parameter expansion removes all content after first slash. + DOWNLOAD_MODULE="${DOWNLOAD_PATH%%/*}" + DOWNLOAD_SRC="ftp.ncbi.nlm.nih.gov::$DOWNLOAD_PATH" + DOWNLOAD_DST="$DOWNLOAD_DIR/$DOWNLOAD_MODULE" + echo "Downloading $DOWNLOAD_SRC to $DOWNLOAD_DST" + rsync --no-motd -DHPRprtv "$DOWNLOAD_SRC" "$DOWNLOAD_DST" +done diff --git a/update/update.sh b/update/update.sh new file mode 100755 index 0000000..a4a1252 --- /dev/null +++ b/update/update.sh @@ -0,0 +1,32 @@ +#!/bin/bash + +set -e + +# Input variables +LOCAL_NCBI_DIR=$(pwd)/ncbi +PREVIOUS_UTA_VERSION=uta_20210129b + +# 1. Download files for SeqRepo and UTA updates +# Input: N/A +# Output: LOCAL_NCBI_DIR populated with downloaded files +mkdir -p $LOCAL_NCBI_DIR +docker build --file Dockerfile.download --tag ncbi:latest --progress plain . +docker run -it --mount type=bind,source=$LOCAL_NCBI_DIR,target=/data/ncbi ncbi:latest + +# 2. Prepare SeqRepo +# Input: TBD +# Output: TBD + +# 3. Prepare UTA +docker build --file ../misc/docker/uta.dockerfile --tag uta:$PREVIOUS_UTA_VERSION --build-arg uta_version=$PREVIOUS_UTA_VERSION ../misc/docker + +# 4. Update UTA and SeqRepo +# Input: LOCAL_NCBI_DIR populated with downloaded files +# Input: PREVIOUS_UTA_VERSION +# Output: Updated SeqRepo (updated in place) +# Output: Updated UTA as a database dump +docker build --file ../Dockerfile --tag uta-update:latest .. +previous_uta_version=$PREVIOUS_UTA_VERSION docker compose -f docker-compose.uta.yml run --rm uta-update +docker compose -f docker-compose.uta.yml down + +# 5. Publish UTA and SeqRepo From ae1715f75bbceb43cdf4dc611fe487700062b7e6 Mon Sep 17 00:00:00 2001 From: nvta1209 <162694616+nvta1209@users.noreply.github.com> Date: Mon, 18 Mar 2024 14:48:15 -0700 Subject: [PATCH 18/54] feat(IPVC-2247): refine UTA update process (#9) --- Dockerfile | 14 ++- README.md | 125 ++++++++++------------- docker-compose.yml | 26 +++++ etc/scripts/run-uta-build.sh | 41 +++++--- misc/docker/ncbi-download.dockerfile | 4 + pyproject.toml | 2 +- update/download.sh => sbin/ncbi-download | 12 ++- sbin/ncbi-download-docker | 22 ++++ sbin/ncbi_parse_genomic_gff.py | 0 sbin/seqrepo-download | 53 ++++++++++ sbin/uta-update | 67 ++++++++++++ update/Dockerfile.download | 9 -- update/docker-compose.uta.yml | 30 ------ update/update.sh | 32 ------ 14 files changed, 275 insertions(+), 162 deletions(-) create mode 100644 docker-compose.yml create mode 100644 misc/docker/ncbi-download.dockerfile rename update/download.sh => sbin/ncbi-download (70%) create mode 100755 sbin/ncbi-download-docker mode change 100644 => 100755 sbin/ncbi_parse_genomic_gff.py create mode 100755 sbin/seqrepo-download create mode 100755 sbin/uta-update delete mode 100644 update/Dockerfile.download delete mode 100644 update/docker-compose.uta.yml delete mode 100755 update/update.sh diff --git a/Dockerfile b/Dockerfile index c65c41d..d62cb3a 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM ubuntu:22.04 +FROM ubuntu:22.04 as uta # set python version and define arguments ARG python_version="3.10" @@ -15,6 +15,14 @@ RUN pip install --upgrade setuptools RUN pip install pysam WORKDIR /opt/repos/uta/ -COPY pyproject.toml . +COPY pyproject.toml ./ +COPY etc ./etc +COPY sbin ./sbin +COPY src ./src RUN pip install -e .[dev] -COPY . . + + +# UTA test image +FROM uta as uta-test +COPY tests ./tests +RUN pip install -e .[test] diff --git a/README.md b/README.md index 492929e..193daab 100644 --- a/README.md +++ b/README.md @@ -284,75 +284,62 @@ To develop UTA, follow these steps. 3. Run container and tests - $ docker run -it --rm uta-build uta:latest - $ python -m unittest - -## Running local UTA build - -### Docker -To run local build of UTA you can follow these steps... - -1. Pick a UTA version as the base (ex. "uta_20210129b") - -2. Setup local UTA Postgresql database Docker container by following the steps above - (`Installing with Docker`). __NOTE__...It takes about 5 minutes before the local - database is ready. + $ docker run -it --rm uta bash -3. Setup local SeqRepo Docker volume. This can be done by running the following two - commands. __NOTE__...this step does require around ~13Gb of local disk space and - takes roughly 30 minutes to complete. You need to wait until the `Docker run` - command completes before moving to the next step. +4. Testing - $ docker pull biocommons/seqrepo - $ docker run --name seqrepo biocommons/seqrepo - -4. Build the image - - $ docker build -t uta-build . - -5. Run UTA build. Supplying a local path with data needed for the workflow is a requirement. + $ docker build --target uta-test -t uta-test . + $ docker run -it --rm uta-test bash + $ python -m unittest - $ docker run \ - -it \ - --rm \ - --name uta-build \ - --volume .:/opt/repos/uta \ - --volume :/temp \ - --volumes-from seqrepo \ - --network=host uta-build:latest - -6. Once in the container you can run this script to create new schema, process input files, - update the database, and dump a new artifact. - - $ bash etc/scripts/run-uta-build.sh - - This update script is dependent on the following directory structure. - - $ tree . - . - ├── gene - │ └── DATA - │ ├── GENE_INFO - │ │ └── Mammalia - │ │ └── Homo_sapiens.gene_info.gz - │ └── gene2accession.gz - ├── genomes - │ └── refseq - │ └── vertebrate_mammalian - │ └── Homo_sapiens - │ └── all_assembly_versions - │ └── GCF_000001405.25_GRCh37.p13 - │ ├── GCF_000001405.25_GRCh37.p13_genomic.fna.gz - │ └── GCF_000001405.25_GRCh37.p13_genomic.gff.gz - └── refseq - └── H_sapiens - └── mRNA_Prot - ├── human.1.protein.faa.gz - ├── human.1.rna.fna.gz - └── human.1.rna.gbff.gz - -## Updating UTA - -To update UTA and SeqRepo, run `update.sh` from the `update` directory. - -The only dependencies of the script are bash and docker. +## UTA update procedure + +### 1. Download files from NCBI + +Run `sbin/ncbi-download-docker`. Requires bash and docker. + +Example: +``` +sbin/ncbi-download-docker $(pwd)/ncbi-data +``` + +The specified directory will have the following structure: + + ├── gene + │ └── DATA + │ ├── GENE_INFO + │ │ └── Mammalia + │ │ └── Homo_sapiens.gene_info.gz + │ └── gene2accession.gz + ├── genomes + │ └── refseq + │ └── vertebrate_mammalian + │ └── Homo_sapiens + │ └── all_assembly_versions + │ └── GCF_000001405.25_GRCh37.p13 + │ ├── GCF_000001405.25_GRCh37.p13_genomic.fna.gz + │ └── GCF_000001405.25_GRCh37.p13_genomic.gff.gz + └── refseq + └── H_sapiens + └── mRNA_Prot + ├── human.1.protein.faa.gz + ├── human.1.rna.fna.gz + └── human.1.rna.gbff.gz + +### 2. Download SeqRepo data + +Run `sbin/seqrepo-download`. Requires bash and docker. + +Example: +``` +sbin/seqrepo-download 2024-02-20 $(pwd)/seqrepo-data +``` + +### 3. Update UTA and SeqRepo + +Run `sbin/uta-update`. Requires bash and docker. + +Example: +``` +sbin/uta-update $(pwd)/ncbi-data $(pwd)/seqrepo-data uta_20210129b 2021-01-29 +``` diff --git a/docker-compose.yml b/docker-compose.yml new file mode 100644 index 0000000..856a5ae --- /dev/null +++ b/docker-compose.yml @@ -0,0 +1,26 @@ +# docker compose file for the UTA update procedure + +version: '3' + +services: + uta: + container_name: uta + image: biocommons/uta:${UTA_VERSION} + environment: + - POSTGRES_HOST_AUTH_METHOD=trust + healthcheck: + test: psql -h localhost -U anonymous -d uta -c "select * from ${UTA_VERSION}.meta" + interval: 10s + retries: 60 + network_mode: host + uta-update: + image: uta-update + command: etc/scripts/run-uta-build.sh ${UTA_VERSION} ${SEQREPO_VERSION} /ncbi-dir /workdir + depends_on: + uta: + condition: service_healthy + volumes: + - ${NCBI_DIR}:/ncbi-dir + - ${SEQREPO_DIR}:/usr/local/share/seqrepo + - ${WORKING_DIR}:/workdir + network_mode: host diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh index 697b57f..504a087 100755 --- a/etc/scripts/run-uta-build.sh +++ b/etc/scripts/run-uta-build.sh @@ -1,21 +1,28 @@ #!/bin/bash -if [ "$#" -lt 2 ] -then - echo "error : too few arguments, you provided $#, 2 required" - echo "usage: run_uta_build_gene.sh " - exit 1 -fi +# source_uta_v is the UTA version before the update. +# seqrepo_data_release is the SeqRepo version before the update. +# ncbi_dir is where the script looks for NCBI data files. +# working_dir stores log files, intermediate data files, and the final database dump. + +set -euxo pipefail + source_uta_v=$1 seqrepo_data_release=$2 +ncbi_dir=$3 +working_dir=$4 -set -euxo pipefail +if [ -z "$source_uta_v" ] || [ -z "$seqrepo_data_release" ] || [ -z "$ncbi_dir" ] || [ -z "$working_dir" ] +then + echo 'Usage: run-uta-build.sh ' + exit 1 +fi # set local variables and create working directories loading_uta_v="uta_1_1" -loading_dir="/temp/loading" -dumps_dir="/temp/dumps" -logs_dir="/temp/logs" +loading_dir="$working_dir/loading" +dumps_dir="$working_dir/dumps" +logs_dir="$working_dir/logs" for d in "$loading_dir" "$dumps_dir" "$logs_dir"; do mkdir -p "$d" done @@ -26,27 +33,27 @@ etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_uta_v" ## Load SeqRepo with new sequences seqrepo load -n NCBI -i "$seqrepo_data_release" \ - /temp/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz \ - /temp/refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz \ - /temp/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.fna.gz 2>& 1 | \ + $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz \ + $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz \ + $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.fna.gz 2>& 1 | \ tee "$logs_dir/seqrepo-load.log" ### extract meta data # genes -sbin/ncbi-parse-geneinfo /temp/gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz | \ +sbin/ncbi-parse-geneinfo $ncbi_dir/gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz | \ gzip -c > "$loading_dir/genes.geneinfo.gz" 2>&1 | tee "$logs_dir/ncbi-parse-geneinfo.log" # transcript protein associations -sbin/ncbi-parse-gene2refseq /temp/gene/DATA/gene2accession.gz | gzip -c > "$loading_dir/assocacs.gz" 2>&1 | \ +sbin/ncbi-parse-gene2refseq $ncbi_dir/gene/DATA/gene2accession.gz | gzip -c > "$loading_dir/assocacs.gz" 2>&1 | \ tee "$logs_dir/ncbi-fetch-assoc-acs" # parse transcript info from GBFF input files -GBFF_files=$(ls /temp/refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz) +GBFF_files=$(ls $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz) sbin/ncbi-parse-gbff "$GBFF_files" | gzip -c > "$loading_dir/gbff.txinfo.gz" 2>&1 | \ tee "$logs_dir/ncbi-parse-gbff.log" # parse alignments from GFF input files -GFF_files=$(ls /temp/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.gff.gz) +GFF_files=$(ls $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.gff.gz) sbin/ncbi_parse_genomic_gff.py "$GFF_files" | gzip -c > "$loading_dir/gff.exonsets.gz" 2>&1 | \ tee "$logs_dir/ncbi-parse-genomic-gff.log" diff --git a/misc/docker/ncbi-download.dockerfile b/misc/docker/ncbi-download.dockerfile new file mode 100644 index 0000000..8da25e8 --- /dev/null +++ b/misc/docker/ncbi-download.dockerfile @@ -0,0 +1,4 @@ +FROM alpine:latest +RUN apk update +RUN apk add bash +RUN apk add rsync \ No newline at end of file diff --git a/pyproject.toml b/pyproject.toml index 89b2d3d..d7d0d9b 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,7 +3,7 @@ name = "uta" dynamic = ["version"] description = "Universal Transcript Archive" readme = "README.md" -requires-python = ">=3.5" +requires-python = ">=3.9" license = {text = "Apache-2.0"} keywords = [ diff --git a/update/download.sh b/sbin/ncbi-download similarity index 70% rename from update/download.sh rename to sbin/ncbi-download index ef3a0bf..61a6874 100755 --- a/update/download.sh +++ b/sbin/ncbi-download @@ -1,4 +1,6 @@ -#!/bin/bash +#!/usr/bin/env bash + +# This script downloads the files needed for a UTA+SeqRepo update into to the given directory. set -e @@ -9,6 +11,14 @@ DOWNLOAD_PATHS=( 'refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz' ) +if [ -z "$DOWNLOAD_DIR" ] +then + echo 'Usage: sbin/ncbi-download ' + exit 1 +else + echo "Downloading files to $DOWNLOAD_DIR" +fi + for DOWNLOAD_PATH in "${DOWNLOAD_PATHS[@]}" do # each top-level directory in NCBI is an rsync module. diff --git a/sbin/ncbi-download-docker b/sbin/ncbi-download-docker new file mode 100755 index 0000000..f1b2de2 --- /dev/null +++ b/sbin/ncbi-download-docker @@ -0,0 +1,22 @@ +#!/usr/bin/env bash + +# This script runs ncbi-download in a docker container. + +DOWNLOAD_DIR=$1 + +if [ -z "$DOWNLOAD_DIR" ] +then + echo 'Usage: sbin/ncbi-download-docker ' + exit 1 +else + echo "Downloading files to $DOWNLOAD_DIR" +fi + +if [[ $DOWNLOAD_DIR != /* ]] +then + echo 'Download directory must be an absolute path' + exit 1 +fi + +docker build --file $(pwd)/misc/docker/ncbi-download.dockerfile --tag ncbi-download --progress plain . +docker run -it -v $(pwd)/sbin/ncbi-download:/dl-script -v $DOWNLOAD_DIR:/output-dir ncbi-download /dl-script /output-dir diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py old mode 100644 new mode 100755 diff --git a/sbin/seqrepo-download b/sbin/seqrepo-download new file mode 100755 index 0000000..0773030 --- /dev/null +++ b/sbin/seqrepo-download @@ -0,0 +1,53 @@ +#!/usr/bin/env bash + +# This script downloads SeqRepo into the given directory. +# Note: pulling data takes ~30 minutes and requires ~13 GB. +# Note: a container called seqrepo will be left behind. +# The name of the container can be changed by providing a third argument. + +set -e + +SEQREPO_VERSION=$1 +OUTPUT_DIR=$2 +# optional: +SEQREPO_CONTAINER_NAME=$3 + +if [ -z "$SEQREPO_VERSION" ] || [ -z "$OUTPUT_DIR" ] +then + echo 'Usage: sbin/seqrepo-download ' + exit 1 +else + echo "SeqRepo data for version $SEQREPO_VERSION will be available in $OUTPUT_DIR" +fi + +# Name of seqrepo container +if [ -z "$SEQREPO_CONTAINER_NAME" ] +then + SEQREPO_CONTAINER_NAME=seqrepo +fi + +if [[ $OUTPUT_DIR != /* ]] +then + echo 'Output directory must be an absolute path' + exit 1 +fi + +if [ ! -d "$OUTPUT_DIR" ]; then + echo "Directory $OUTPUT_DIR does not exist." + exit 1 +fi + +# Pull seqrepo image +docker pull biocommons/seqrepo:$SEQREPO_VERSION + +# Download seqrepo data using seqrepo image +if docker ps -aq -f name=$SEQREPO_CONTAINER_NAME +then + echo "Container called $SEQREPO_CONTAINER_NAME already exists. Skipping seqrepo data download." +else + docker run --name seqrepo biocommons/seqrepo:$SEQREPO_VERSION +fi + +# Copy seqrepo data into a local directory +echo "Copying seqrepo data into $OUTPUT_DIR ..." +docker run -it -v $OUTPUT_DIR:/output-dir --volumes-from $SEQREPO_CONTAINER_NAME:ro ubuntu bash -c 'cp -R /usr/local/share/seqrepo/* /output-dir' diff --git a/sbin/uta-update b/sbin/uta-update new file mode 100755 index 0000000..b0a2667 --- /dev/null +++ b/sbin/uta-update @@ -0,0 +1,67 @@ +#!/usr/bin/env bash + +# This script runs the UTA update procedure. +# It updates the specified UTA and SeqRepo using the given NCBI files. +# It produces a postgres dump of the updated UTA database and an updated SeqRepo (updated in place). +# It expects to be run from the root of the uta repository. + +set -e + +# export environment variables for docker compose file +export NCBI_DIR=$1 +export SEQREPO_DIR=$2 +export WORKING_DIR=$3 +export UTA_VERSION=$4 +export SEQREPO_VERSION=$5 + +if [ -z "$NCBI_DIR" ] || [ -z "$SEQREPO_DIR" ] || [ -z "$WORKING_DIR" ] || [ -z "$UTA_VERSION" ] || [ -z "$SEQREPO_VERSION" ] +then + echo 'Usage: sbin/uta-update ' + exit 1 +else + echo "Updating UTA and SeqRepo using files in $NCBI_DIR and SeqRepo data in $SEQREPO_DIR" + echo "Starting from UTA version $UTA_VERSION and SeqRepo version $SEQREPO_VERSION" + echo "Logs and intermediate files will be available in $WORKING_DIR" +fi + +# Ensure directories are compatible with docker volume usage +if [[ $NCBI_DIR != /* ]] && [[ $NCBI_DIR != .* ]] +then + echo 'NCBI file directory must start with / or .' + exit 1 +fi + +if [[ $SEQREPO_DIR != /* ]] && [[ $SEQREPO_DIR != .* ]] +then + echo 'SeqRepo data directory must start with / or .' + exit 1 +fi + +if [[ $WORKING_DIR != /* ]] && [[ $WORKING_DIR != .* ]] +then + echo 'Working directory must start with / or .' + exit 1 +fi + +# Ensure directories exist. +if [ ! -d "$NCBI_DIR" ]; then + echo "Directory $NCBI_DIR does not exist." + exit 1 +fi + +if [ ! -d "$SEQREPO_DIR" ]; then + echo "Directory $SEQREPO_DIR does not exist." + exit 1 +fi + +if [ ! -d "$WORKING_DIR" ]; then + echo "Directory $WORKING_DIR does not exist." + exit 1 +fi + +# Build the UTA image. +docker build -t uta-update . + +# Bring up a UTA database and run the UTA update procedure. +# docker compose doesn't respect the container name specified in the compose file, so container name is specified here +docker compose run --rm --name uta-update uta-update diff --git a/update/Dockerfile.download b/update/Dockerfile.download deleted file mode 100644 index e2481e4..0000000 --- a/update/Dockerfile.download +++ /dev/null @@ -1,9 +0,0 @@ -FROM debian:stable-slim - -RUN apt-get -y update -RUN apt-get -y install rsync - -WORKDIR /download_ncbi -VOLUME [ "/data/ncbi" ] -COPY download.sh ./ -CMD ./download.sh /data/ncbi diff --git a/update/docker-compose.uta.yml b/update/docker-compose.uta.yml deleted file mode 100644 index 6018725..0000000 --- a/update/docker-compose.uta.yml +++ /dev/null @@ -1,30 +0,0 @@ -version: '3' - -services: - seqrepo: - container_name: seqrepo - image: ubuntu:latest - command: echo 'Start SeqRepo, which is required for UTA update' - network_mode: host - uta: - container_name: uta - image: uta:${previous_uta_version} - environment: - - POSTGRES_HOST_AUTH_METHOD=trust - healthcheck: - test: pg_isready && psql -h localhost -U anonymous -d uta -c "select * from ${previous_uta_version}.meta" - interval: 10s - retries: 60 - network_mode: host - uta-update: - container_name: uta-update - image: uta-update:latest - command: etc/scripts/run-uta-build.sh ${previous_uta_version} - depends_on: - previous-uta: - condition: service_healthy - # seqrepo: - # condition: service_healthy - volumes: - - ./ncbi:/temp - network_mode: host diff --git a/update/update.sh b/update/update.sh deleted file mode 100755 index a4a1252..0000000 --- a/update/update.sh +++ /dev/null @@ -1,32 +0,0 @@ -#!/bin/bash - -set -e - -# Input variables -LOCAL_NCBI_DIR=$(pwd)/ncbi -PREVIOUS_UTA_VERSION=uta_20210129b - -# 1. Download files for SeqRepo and UTA updates -# Input: N/A -# Output: LOCAL_NCBI_DIR populated with downloaded files -mkdir -p $LOCAL_NCBI_DIR -docker build --file Dockerfile.download --tag ncbi:latest --progress plain . -docker run -it --mount type=bind,source=$LOCAL_NCBI_DIR,target=/data/ncbi ncbi:latest - -# 2. Prepare SeqRepo -# Input: TBD -# Output: TBD - -# 3. Prepare UTA -docker build --file ../misc/docker/uta.dockerfile --tag uta:$PREVIOUS_UTA_VERSION --build-arg uta_version=$PREVIOUS_UTA_VERSION ../misc/docker - -# 4. Update UTA and SeqRepo -# Input: LOCAL_NCBI_DIR populated with downloaded files -# Input: PREVIOUS_UTA_VERSION -# Output: Updated SeqRepo (updated in place) -# Output: Updated UTA as a database dump -docker build --file ../Dockerfile --tag uta-update:latest .. -previous_uta_version=$PREVIOUS_UTA_VERSION docker compose -f docker-compose.uta.yml run --rm uta-update -docker compose -f docker-compose.uta.yml down - -# 5. Publish UTA and SeqRepo From b0d4c2cf02c25f08bcd95a63a8ea35a9882e3633 Mon Sep 17 00:00:00 2001 From: nvta1209 <162694616+nvta1209@users.noreply.github.com> Date: Mon, 18 Mar 2024 17:40:33 -0700 Subject: [PATCH 19/54] feat(IPVC-2247): reuse main dockerfile for ncbi downloads (#10) --- misc/docker/ncbi-download.dockerfile | 4 ---- sbin/ncbi-download-docker | 4 +++- sbin/uta-update | 2 +- 3 files changed, 4 insertions(+), 6 deletions(-) delete mode 100644 misc/docker/ncbi-download.dockerfile diff --git a/misc/docker/ncbi-download.dockerfile b/misc/docker/ncbi-download.dockerfile deleted file mode 100644 index 8da25e8..0000000 --- a/misc/docker/ncbi-download.dockerfile +++ /dev/null @@ -1,4 +0,0 @@ -FROM alpine:latest -RUN apk update -RUN apk add bash -RUN apk add rsync \ No newline at end of file diff --git a/sbin/ncbi-download-docker b/sbin/ncbi-download-docker index f1b2de2..30d4f63 100755 --- a/sbin/ncbi-download-docker +++ b/sbin/ncbi-download-docker @@ -2,6 +2,8 @@ # This script runs ncbi-download in a docker container. +set -e + DOWNLOAD_DIR=$1 if [ -z "$DOWNLOAD_DIR" ] @@ -18,5 +20,5 @@ then exit 1 fi -docker build --file $(pwd)/misc/docker/ncbi-download.dockerfile --tag ncbi-download --progress plain . +docker build --target uta -t ncbi-download --progress plain . docker run -it -v $(pwd)/sbin/ncbi-download:/dl-script -v $DOWNLOAD_DIR:/output-dir ncbi-download /dl-script /output-dir diff --git a/sbin/uta-update b/sbin/uta-update index b0a2667..23d93af 100755 --- a/sbin/uta-update +++ b/sbin/uta-update @@ -60,7 +60,7 @@ if [ ! -d "$WORKING_DIR" ]; then fi # Build the UTA image. -docker build -t uta-update . +docker build --target uta -t uta-update . # Bring up a UTA database and run the UTA update procedure. # docker compose doesn't respect the container name specified in the compose file, so container name is specified here From 0c23e73700c7771f44e079ba2a3608bfe707f33c Mon Sep 17 00:00:00 2001 From: Paige Date: Wed, 20 Mar 2024 09:17:07 -0700 Subject: [PATCH 20/54] feat(IPVC-2230): make changes to formats (#7) --- bin/run_uta_build_gene.sh | 26 ----------------- bin/run_uta_parse_gff.sh | 29 ------------------- etc/scripts/run-uta-build.sh | 5 +++- .../data/splign-manual/generate-loading-data | 12 ++++---- sbin/ncbi-parse-gbff | 20 +++++++++---- sbin/ncbi-parse-gene2refseq | 4 +-- sbin/ncbi-parse-geneinfo | 1 + src/uta/formats/geneaccessions.py | 2 +- src/uta/formats/geneinfo.py | 2 +- src/uta/formats/txinfo.py | 2 +- src/uta/loading.py | 9 +++--- 11 files changed, 36 insertions(+), 76 deletions(-) delete mode 100755 bin/run_uta_build_gene.sh delete mode 100755 bin/run_uta_parse_gff.sh diff --git a/bin/run_uta_build_gene.sh b/bin/run_uta_build_gene.sh deleted file mode 100755 index 73287eb..0000000 --- a/bin/run_uta_build_gene.sh +++ /dev/null @@ -1,26 +0,0 @@ -#!/bin/bash - -export uta_v=uta_20210129b - -## get the file -rsync --no-motd -HRavP ftp.ncbi.nlm.nih.gov::gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz . - -## extract meta data -sbin/ncbi-parse-geneinfo DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz | gzip -c > genes.geneinfo.gz - -### pull a uta database -docker pull biocommons/uta:$uta_v - -### stand up container for UTA database -docker run -d -v /tmp:/tmp -v uta_vol:/var/lib/postgresql/data --name $uta_v --network=host biocommons/uta:$uta_v -sleep 5 -docker ps - -## update the uta database -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo genes.sample.gz - -## psql_dump -pg_dump -U uta_admin -h localhost -d uta -t uta_20210129b.gene | gzip -c > uta.pgd.gz -## stop local postgres container -docker stop uta_20210129b -docker rm uta_20210129b diff --git a/bin/run_uta_parse_gff.sh b/bin/run_uta_parse_gff.sh deleted file mode 100755 index c42cc5e..0000000 --- a/bin/run_uta_parse_gff.sh +++ /dev/null @@ -1,29 +0,0 @@ -#!/bin/bash - -export uta_v=uta_20210129b - -## get the file -rsync --no-motd -HRavP --no-relative ftp.ncbi.nlm.nih.gov::genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.25_GRCh37.p13/GCF_000001405.25_GRCh37.p13_genomic.gff.gz . - - -## extract exon alignments -python sbin/ncbi_parse_genomic_gff.py GCF_000001405.25_GRCh37.p13_genomic.gff.gz - - -### pull a uta database -docker pull biocommons/uta:$uta_v - -### stand up container for UTA database -docker run -d -v /tmp:/tmp -v uta_vol:/var/lib/postgresql/data --name $uta_v --network=host biocommons/uta:$uta_v -sleep 5 -docker ps - -## update the uta database -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset ncbi-gff.exonset.gz - - -## psql_dump -pg_dump -U uta_admin -h localhost -d uta -t uta_20210129b.gene | gzip -c > uta.pgd.gz -## stop local postgres container -docker stop uta_20210129b -docker rm uta_20210129b diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh index 504a087..7ec151b 100755 --- a/etc/scripts/run-uta-build.sh +++ b/etc/scripts/run-uta-build.sh @@ -61,7 +61,6 @@ sbin/ncbi_parse_genomic_gff.py "$GFF_files" | gzip -c > "$loading_dir/gff.exonse sbin/exonset-to-seqinfo -o NCBI "$loading_dir/gff.exonsets.gz" | gzip -c > "$loading_dir/seqinfo.gz" 2>&1 | \ tee "$logs_dir/exonset-to-seqinfo.log" - ### update the uta database # genes uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$loading_dir/genes.geneinfo.gz" 2>&1 | \ @@ -83,5 +82,9 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$load sbin/uta-diff "$source_uta_v" "$loading_uta_v" +# exon alignments +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$loading_dir/ncbi-gff.exonset.gz" 2>&1 | \ + tee "$logs_dir/load-exonset.log" + ### psql_dump pg_dump -U uta_admin -h localhost -d uta -t "$loading_uta_v.gene" | gzip -c > "$dumps_dir/uta.pgd.gz" diff --git a/loading/data/splign-manual/generate-loading-data b/loading/data/splign-manual/generate-loading-data index 86744ed..c904f3e 100755 --- a/loading/data/splign-manual/generate-loading-data +++ b/loading/data/splign-manual/generate-loading-data @@ -61,11 +61,12 @@ def parse_splign(fn, txdata): try: txd = txdata[tx_ac] except KeyError: - raise KeyError(f"{tx_ac}: no cds or hgnc info in {txdata_fn}") + raise KeyError(f"{tx_ac}: no cds or gene_symbol info in {txdata_fn}") - hgnc = txd["hgnc"] - if hgnc is None: - _logger.warn(f"No HGNC symbol in {txdata_fn} for {tx_ac}") + gene_symbol = txd["hgnc"] + gene_id = txd.get("gene_id") + if gene_symbol is None: + _logger.warning(f"No gene symbol in {txdata_fn} for {tx_ac}") cds = txd["cds"] if cds is None: @@ -79,7 +80,8 @@ def parse_splign(fn, txdata): txinfo = uta.formats.txinfo.TxInfo( origin=origin, ac=tx_ac, - hgnc=hgnc, + gene_id=gene_id, + gene_symbol=gene_symbol, cds_se_i=cds_se_i, exons_se_i=tx_exons_str) exonset = uta.formats.exonset.ExonSet( diff --git a/sbin/ncbi-parse-gbff b/sbin/ncbi-parse-gbff index 45897c6..c650d3b 100755 --- a/sbin/ncbi-parse-gbff +++ b/sbin/ncbi-parse-gbff @@ -9,8 +9,8 @@ See uta.formats for a description of those file formats. In a nutshell, this means that you'll get data like this: ncbi.txinfo.gz: -origin ac hgnc cds_se_i exons_se_i -NCBI RefSeq NM_053283.2 DCD 62,395 0,120;120,159;159,261;261,351;351,517 +origin ac gene_id gene_symbol cds_se_i exons_se_i +NCBI RefSeq NM_053283.2 117159 DCD 62,395 0,120;120,159;159,261;261,351;351,517 ncbi.exonsets.gz: tx_ac alt_ac method strand exons_se_i @@ -72,12 +72,21 @@ class SeqRecordFacade(Bio.SeqRecord.SeqRecord): return self._sr.id @property - def hgnc(self): + def gene_symbol(self): genes = [f for f in self._sr.features if f.type == "gene"][ 0].qualifiers["gene"] assert len(genes) == 1 return genes[0] + @property + def gene_id(self): + # db_xref="GeneID:1234" + db_xrefs = [f for f in self._sr.features if f.type == "gene"][ + 0].qualifiers["db_xref"] + gene_ids = [x.partition(":")[2] for x in db_xrefs if x.startswith("GeneID:")] + assert len(gene_ids) == 1 + return gene_ids[0] + @property def cds_se_i(self): try: @@ -149,14 +158,15 @@ if __name__ == "__main__": cds_se_i = srf.cds_se_i ti = TxInfo(ac=srf.id, origin=opts.origin, - hgnc=srf.hgnc, + gene_id=srf.gene_id, + gene_symbol=srf.gene_symbol, cds_se_i=None if cds_se_i is None else "{},{}".format( *cds_se_i), exons_se_i=";".join( ["{},{}".format(*ese) for ese in srf.exons_se_i]) ) tiw.write(ti) - genes.add(srf.hgnc) + genes.add(srf.gene_symbol) logger.info("{ng} genes in {fn} ({c})".format(ng=len(genes), fn=fn, c=prefixes)) total_genes ^= genes all_prefixes += prefixes diff --git a/sbin/ncbi-parse-gene2refseq b/sbin/ncbi-parse-gene2refseq index 5d11206..7bfe70c 100755 --- a/sbin/ncbi-parse-gene2refseq +++ b/sbin/ncbi-parse-gene2refseq @@ -38,14 +38,14 @@ if __name__ == "__main__": if rec["rna_nucleotide_accession.version"] == "-" and rec["protein_accession.version"] == "-": continue - ga = GeneAccessions(hgnc=rec["symbol"], + ga = GeneAccessions(gene_symbol=rec["symbol"], tx_ac=rec["rna_nucleotide_accession.version"], gene_id=rec["geneid"], pro_ac=rec["protein_accession.version"], origin="NCBI", ) - key = (ga.hgnc, ga.tx_ac, ga.gene_id, ga.pro_ac) + key = (ga.gene_symbol, ga.tx_ac, ga.gene_id, ga.pro_ac) if key in seen: continue diff --git a/sbin/ncbi-parse-geneinfo b/sbin/ncbi-parse-geneinfo index 73966b8..3dd2534 100755 --- a/sbin/ncbi-parse-geneinfo +++ b/sbin/ncbi-parse-geneinfo @@ -34,6 +34,7 @@ if __name__ == "__main__": continue gi = GeneInfo( tax_id=rec["tax_id"], + gene_symbol=rec["symbol"], gene_id=rec["geneid"], hgnc=rec["symbol_from_nomenclature_authority"], maploc=rec["map_location"], diff --git a/src/uta/formats/geneaccessions.py b/src/uta/formats/geneaccessions.py index 9837d82..3b5985b 100644 --- a/src/uta/formats/geneaccessions.py +++ b/src/uta/formats/geneaccessions.py @@ -3,7 +3,7 @@ class GeneAccessions(recordtype.recordtype('GeneAccessions', - ['hgnc', 'tx_ac', 'gene_id', 'pro_ac', 'origin'])): + ['gene_symbol', 'tx_ac', 'gene_id', 'pro_ac', 'origin'])): pass diff --git a/src/uta/formats/geneinfo.py b/src/uta/formats/geneinfo.py index f4a6015..c226452 100644 --- a/src/uta/formats/geneinfo.py +++ b/src/uta/formats/geneinfo.py @@ -5,7 +5,7 @@ class GeneInfo(recordtype.recordtype('GeneInfo', - ['gene_id', 'tax_id', 'hgnc', 'maploc', 'aliases', 'type', 'summary', 'descr', 'xrefs'])): + ['gene_id', 'gene_symbol', 'tax_id', 'hgnc', 'maploc', 'aliases', 'type', 'summary', 'descr', 'xrefs'])): pass diff --git a/src/uta/formats/txinfo.py b/src/uta/formats/txinfo.py index f8d6cf5..fa811fd 100644 --- a/src/uta/formats/txinfo.py +++ b/src/uta/formats/txinfo.py @@ -3,7 +3,7 @@ class TxInfo(recordtype.recordtype('TxInfo', - ['origin', 'ac', 'hgnc', 'cds_se_i', 'exons_se_i'])): + ['origin', 'ac', 'gene_id', 'gene_symbol', 'cds_se_i', 'exons_se_i'])): pass diff --git a/src/uta/loading.py b/src/uta/loading.py index e04a97a..04c61e7 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -656,17 +656,16 @@ def _fetch_origin_by_name(name): u_tx = usam.Transcript( ac=ti.ac, origin=ori, - hgnc=ti.hgnc, + hgnc=ti.gene_symbol, cds_start_i=cds_start_i, cds_end_i=cds_end_i, cds_md5=cds_md5, ) session.add(u_tx) - - if u_tx.hgnc != ti.hgnc: - logger.warn("{ti.ac}: HGNC symbol changed from {u_tx.hgnc} to {ti.hgnc}".format( + if u_tx.hgnc != ti.gene_symbol: + logger.warn("{ti.ac}: HGNC symbol changed from {u_tx.hgnc} to {ti.gene_symbol}".format( u_tx=u_tx, ti=ti)) - u_tx.hgnc = ti.hgnc + u_tx.hgnc = ti.gene_symbol # state: transcript now exists, either existing or freshly-created From 3dece55f666d02e948f3293bba3eca5ee391193d Mon Sep 17 00:00:00 2001 From: Paige Date: Wed, 20 Mar 2024 09:38:27 -0700 Subject: [PATCH 21/54] feat(IPVC-2229): add utility to download from eutils (#11) --- src/uta/exceptions.py | 4 +++ src/uta/tools/eutils.py | 35 +++++++++++++++++++++++++ tests/test_uta_tools_eutils.py | 47 ++++++++++++++++++++++++++++++++++ 3 files changed, 86 insertions(+) create mode 100644 src/uta/tools/eutils.py create mode 100644 tests/test_uta_tools_eutils.py diff --git a/src/uta/exceptions.py b/src/uta/exceptions.py index 36454a2..a0cad0c 100644 --- a/src/uta/exceptions.py +++ b/src/uta/exceptions.py @@ -17,6 +17,10 @@ class InvalidIntervalError(UTAError): class InvalidHGVSVariantError(UTAError): pass + +class EutilsDownloadError(Exception): + pass + # # Copyright 2014 UTA Contributors (https://bitbucket.org/biocommons/uta) ## diff --git a/src/uta/tools/eutils.py b/src/uta/tools/eutils.py new file mode 100644 index 0000000..7abfaa4 --- /dev/null +++ b/src/uta/tools/eutils.py @@ -0,0 +1,35 @@ +from enum import Enum + +import requests + +from uta import EutilsDownloadError + + +class NcbiFileFormatEnum(str, Enum): + FASTA = "fasta" + GENBANK = "gb" + + +def download_from_eutils(accession: str, file_format: NcbiFileFormatEnum, output_file: str) -> None: + """ + Download a file from NCBI using the eutils endpoint. + Args: + - accession: NCBI accession ID + - file_format: File format to download ("fasta" or "gb") + - output_file: Path to the file where the downloaded content will be saved + """ + + base_url = "https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi" + params = { + "db": "nuccore", + "id": accession, + "retmode": "text", + "rettype": file_format + } + response = requests.get(base_url, params=params) + + if response.status_code == 200: + with open(output_file, 'w') as file: + file.write(response.text) + else: + raise EutilsDownloadError(f"Failed to download {file_format} file for {accession}. HTTP status code: {response.status_code}") \ No newline at end of file diff --git a/tests/test_uta_tools_eutils.py b/tests/test_uta_tools_eutils.py new file mode 100644 index 0000000..c875a7f --- /dev/null +++ b/tests/test_uta_tools_eutils.py @@ -0,0 +1,47 @@ +import os +import unittest +from unittest.mock import Mock, patch + +from uta import EutilsDownloadError +from uta.tools.eutils import download_from_eutils, NcbiFileFormatEnum + + +class TestEutils(unittest.TestCase): + URL = 'https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi' + + def setUp(self): + self.output_file = 'test_output.fa' + + def tearDown(self): + if os.path.exists(self.output_file): + os.remove(self.output_file) + + @patch('requests.get') + def test_successful_download(self, mock_get): + mock_response = Mock() + mock_response.status_code = 200 + mock_response.text = 'file content' + mock_get.return_value = mock_response + + download_from_eutils('accession', NcbiFileFormatEnum.FASTA, self.output_file) + + mock_get.assert_called_once_with( + self.URL, + params={ + 'db': 'nuccore', + 'id': 'accession', + 'retmode': 'text', + 'rettype': 'fasta' + } + ) + + with open(self.output_file, 'r') as file: + self.assertEqual(file.read(), 'file content') + + @patch('requests.get') + def test_unsuccessful_download(self, mock_get): + mock_response = Mock() + mock_response.status_code = 404 + mock_get.return_value = mock_response + with self.assertRaises(EutilsDownloadError): + download_from_eutils('accession', NcbiFileFormatEnum.FASTA, self.output_file) From 9bdf64042b1110d4f0a4c23ce680903f43e51da7 Mon Sep 17 00:00:00 2001 From: nvta1209 <162694616+nvta1209@users.noreply.github.com> Date: Thu, 21 Mar 2024 13:54:01 -0700 Subject: [PATCH 22/54] Revive tests (#12) --- Dockerfile | 3 +++ README.md | 2 +- sbin/assoc-acs-merge | 7 +++-- sbin/exonset-to-seqinfo | 2 +- sbin/ncbi-parse-gbff | 7 ++--- src/uta/__init__.py | 2 +- tests/test_uta_models.py | 56 +++++++++++++++++++++------------------- 7 files changed, 40 insertions(+), 39 deletions(-) diff --git a/Dockerfile b/Dockerfile index d62cb3a..ec6d67d 100644 --- a/Dockerfile +++ b/Dockerfile @@ -24,5 +24,8 @@ RUN pip install -e .[dev] # UTA test image FROM uta as uta-test +RUN DEBIAN_FRONTEND=noninteractive apt-get -yq install postgresql COPY tests ./tests RUN pip install -e .[test] +RUN useradd postgres-testing +USER postgres-testing diff --git a/README.md b/README.md index 193daab..f99e8af 100644 --- a/README.md +++ b/README.md @@ -289,7 +289,7 @@ To develop UTA, follow these steps. 4. Testing $ docker build --target uta-test -t uta-test . - $ docker run -it --rm uta-test bash + $ docker run -it --rm -v $(pwd)/tests:/opt/repos/uta/tests -v $(pwd)/src:/opt/repos/uta/src uta-test bash $ python -m unittest ## UTA update procedure diff --git a/sbin/assoc-acs-merge b/sbin/assoc-acs-merge index 7c7ec00..1cfbff9 100755 --- a/sbin/assoc-acs-merge +++ b/sbin/assoc-acs-merge @@ -15,7 +15,6 @@ duplicates""" import csv import gzip import io -import os import sys import attr @@ -43,15 +42,15 @@ if __name__ == "__main__": aars = dict() for fn in sys.argv[1:]: ifh = csv.DictReader(anyopen(fn), delimiter="\t") - + if ofh is None: ofh = csv.DictWriter(sys.stdout, fieldnames=out_header, delimiter="\t") ofh.writeheader() - + for r in ifh: if r["tx_ac"] in ("","-") or r["pro_ac"] in ("","-"): continue - + aar = AssAccRec(tx_ac=r["tx_ac"], pro_ac=r["pro_ac"], origin=r["origin"]) k = (aar.tx_ac, aar.origin) if k in aars: diff --git a/sbin/exonset-to-seqinfo b/sbin/exonset-to-seqinfo index c6c711a..33b3cba 100755 --- a/sbin/exonset-to-seqinfo +++ b/sbin/exonset-to-seqinfo @@ -16,7 +16,7 @@ from bioutils.digests import seq_md5 from biocommons.seqrepo import SeqRepo # from multifastadb import MultiFastaDB -from uta.formats.exonset import ExonSet, ExonSetReader +from uta.formats.exonset import ExonSetReader from uta.formats.seqinfo import SeqInfo, SeqInfoWriter diff --git a/sbin/ncbi-parse-gbff b/sbin/ncbi-parse-gbff index c650d3b..e3d7d13 100755 --- a/sbin/ncbi-parse-gbff +++ b/sbin/ncbi-parse-gbff @@ -28,11 +28,8 @@ import argparse from collections import Counter import gzip import io -import itertools import logging import logging.config -import os -import pprint import pkg_resources import re import sys @@ -41,9 +38,7 @@ import Bio.SeqIO import Bio.SeqRecord from bioutils.digests import seq_md5 -from uta.formats.exonset import ExonSet, ExonSetWriter from uta.formats.txinfo import TxInfo, TxInfoWriter -from uta.formats.geneaccessions import GeneAccessionsReader origin = "NCBI" @@ -116,6 +111,7 @@ def gbff_filter(it): if line.startswith(delim): emit = False + def gbff_block_reader(it): """yield strings, each representing a full genbank record""" delim = "//" @@ -131,6 +127,7 @@ def gbff_block_reader(it): yield SeqRecordFacade(Bio.SeqIO.read(io.StringIO(emit), "gb")) emit = None + if __name__ == "__main__": logging_conf_fn = pkg_resources.resource_filename( "uta", "etc/logging.conf") diff --git a/src/uta/__init__.py b/src/uta/__init__.py index ff05e64..eb783bc 100644 --- a/src/uta/__init__.py +++ b/src/uta/__init__.py @@ -24,7 +24,7 @@ def connect(db_url=default_db_url): """ - Connect to a UTA database instance and return a UTA0 interface instance. + Connect to a UTA database instance and return a sqlalchemy Session. When called with an explicit db_url argument, that db_url is used for connecting. diff --git a/tests/test_uta_models.py b/tests/test_uta_models.py index 86eafea..3a73963 100644 --- a/tests/test_uta_models.py +++ b/tests/test_uta_models.py @@ -1,7 +1,7 @@ -import os import unittest import sqlalchemy +from sqlalchemy import text import testing.postgresql import uta @@ -44,15 +44,17 @@ } -class Test_uta_models(unittest.TestCase): +class TestUtaModels(unittest.TestCase): @classmethod def setUpClass(cls): cls._postgresql = testing.postgresql.Postgresql() engine = sqlalchemy.create_engine(cls._postgresql.url()) - engine.execute('drop schema if exists {schema} cascade'.format(schema=usam.schema_name)) - engine.execute('create schema {schema}'.format(schema=usam.schema_name)) + with engine.connect() as connection: + connection.execute(text('drop schema if exists {schema} cascade'.format(schema=usam.schema_name))) + connection.execute(text('create schema {schema}'.format(schema=usam.schema_name))) + connection.commit() engine.dispose() cls.session = uta.connect(cls._postgresql.url()) @@ -161,7 +163,7 @@ def setUpClass(cls): @classmethod def tearDownClass(cls): - # sqlalchemy is keeping connections open and I can't figure out where + cls.session.close() # kill the database (we started it) import signal cls._postgresql.stop(_signal=signal.SIGKILL) @@ -172,9 +174,9 @@ def test_origin(self): self.assertEqual(len(all_origins), 1) o = all_origins[0] - self.assertRegexpMatches(o.name, 'Testing') - self.assertEquals(o.url, 'http://bogus.com/') - self.assertEquals(o.url_ac_fmt, 'http://bogus.com/{ac}') + self.assertRegex(o.name, 'Testing') + self.assertEqual(o.url, 'http://bogus.com/') + self.assertEqual(o.url_ac_fmt, 'http://bogus.com/{ac}') # NM_000680.2, NM_033302.2, NM_033303.3, NM_033304.2 self.assertEqual(len(o.transcripts), 4) @@ -201,23 +203,23 @@ def test_dnaseq(self): n = self.session.query(usam.SeqAnno).filter( usam.SeqAnno.ac == 'NC_000008.10').one() - self.assertEquals(n.ac, u'NC_000008.10') + self.assertEqual(n.ac, u'NC_000008.10') # self.assertTrue(len(n.exon_sets),2) - self.assertRegexpMatches(n.origin.name, '^Testing') - #self.assertEquals(len(n.transcripts), 0) + self.assertRegex(n.origin.name, '^Testing') + #self.assertEqual(len(n.transcripts), 0) n = self.session.query(usam.SeqAnno).filter( usam.SeqAnno.ac == 'NM_000680.2').one() - self.assertEquals(n.ac, u'NM_000680.2') + self.assertEqual(n.ac, u'NM_000680.2') # self.assertTrue(len(n.exon_sets),1) - self.assertRegexpMatches(n.origin.name, '^Testing') + self.assertRegex(n.origin.name, '^Testing') n = self.session.query(usam.Seq).join(usam.Seq.aliases).filter( usam.SeqAnno.ac == 'NM_000680.2').one() - self.assertEquals(len(n.seq), 2281) + self.assertEqual(len(n.seq), 2281) self.assertTrue(n.seq.startswith('gaattccgaa')) self.assertTrue(n.seq.endswith('gacatttatg')) - #self.assertEquals(len(n.transcripts), 1) + #self.assertEqual(len(n.transcripts), 1) def test_exon_set(self): all_exon_sets = self.session.query(usam.Seq).all() @@ -228,12 +230,12 @@ def test_exon_set(self): # http://www.ncbi.nlm.nih.gov/nuccore/NM_000680.2 ## es = [ es for es in exon_sets if es.is_primary ][0] - ## self.assertEquals( (es.cds_start_i,es.cds_end_i), (436, 1837) ) - ## self.assertEquals( len(es.exons), 2 ) - ## self.assertEquals( es.is_primary, True ) - ## self.assertEquals( es.ref_dnaseq.ac, 'NM_000680.2' ) - ## self.assertEquals( es.strand, 1 ) - ## self.assertEquals( es.transcript.ac, 'NM_000680.2' ) + ## self.assertEqual( (es.cds_start_i,es.cds_end_i), (436, 1837) ) + ## self.assertEqual( len(es.exons), 2 ) + ## self.assertEqual( es.is_primary, True ) + ## self.assertEqual( es.ref_dnaseq.ac, 'NM_000680.2' ) + ## self.assertEqual( es.strand, 1 ) + ## self.assertEqual( es.transcript.ac, 'NM_000680.2' ) # seq_gene.md.gz: # 9606 8 26627222 26627665 - NT_167187.1 14485368 14485811 - NM_000680.2 GeneID:148 UTR GRCh37.p10-Primary Assembly NM_000680.2 - @@ -241,12 +243,12 @@ def test_exon_set(self): # 9606 8 26721604 26722486 - NT_167187.1 14579750 14580632 - NP_000671.2 GeneID:148 CDS GRCh37.p10-Primary Assembly NM_000680.2 - # 9606 8 26722487 26722922 - NT_167187.1 14580633 14581068 - NM_000680.2 GeneID:148 UTR GRCh37.p10-Primary Assembly NM_000680.2 - ## es = [ es for es in exon_sets if not es.is_primary ][0] - ## self.assertEquals( (es.cds_start_i,es.cds_end_i), (26627665, 26722486) ) - ## self.assertEquals( len(es.exons), 2 ) - ## self.assertEquals( es.is_primary, False ) - ## self.assertEquals( es.ref_dnaseq.ac, 'NC_000008.10' ) - ## self.assertEquals( es.strand, -1 ) - ## self.assertEquals( es.transcript.ac, 'NM_000680.2' ) + ## self.assertEqual( (es.cds_start_i,es.cds_end_i), (26627665, 26722486) ) + ## self.assertEqual( len(es.exons), 2 ) + ## self.assertEqual( es.is_primary, False ) + ## self.assertEqual( es.ref_dnaseq.ac, 'NC_000008.10' ) + ## self.assertEqual( es.strand, -1 ) + ## self.assertEqual( es.transcript.ac, 'NM_000680.2' ) def test_exon(self): t = self.session.query(usam.Transcript).filter( From 38851f9434568bd79d17c1e6ee3d6106b7cf1f1a Mon Sep 17 00:00:00 2001 From: Paige Date: Fri, 22 Mar 2024 08:06:18 -0700 Subject: [PATCH 23/54] feat(IPVC-2278): update uta-align (#13) --- README.md | 2 +- etc/scripts/run-uta-build.sh | 8 +------- pyproject.toml | 2 +- src/uta/loading.py | 11 +++++------ 4 files changed, 8 insertions(+), 15 deletions(-) diff --git a/README.md b/README.md index f99e8af..e897474 100644 --- a/README.md +++ b/README.md @@ -341,5 +341,5 @@ Run `sbin/uta-update`. Requires bash and docker. Example: ``` -sbin/uta-update $(pwd)/ncbi-data $(pwd)/seqrepo-data uta_20210129b 2021-01-29 +sbin/uta-update $(pwd)/ncbi-data $(pwd)/seqrepo-data $(pwd)/uta-build uta_20210129b 2024-02-20 ``` diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh index 7ec151b..86175c8 100755 --- a/etc/scripts/run-uta-build.sh +++ b/etc/scripts/run-uta-build.sh @@ -75,16 +75,10 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$load tee "$logs_dir/load-exonsets.log" # align exons -#uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | tee "$logs_dir/align-exons.log" - +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | tee "$logs_dir/align-exons.log" ### run diff sbin/uta-diff "$source_uta_v" "$loading_uta_v" - -# exon alignments -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$loading_dir/ncbi-gff.exonset.gz" 2>&1 | \ - tee "$logs_dir/load-exonset.log" - ### psql_dump pg_dump -U uta_admin -h localhost -d uta -t "$loading_uta_v.gene" | gzip -c > "$dumps_dir/uta.pgd.gz" diff --git a/pyproject.toml b/pyproject.toml index d7d0d9b..357be5a 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -53,7 +53,7 @@ dependencies = [ "pytz", "recordtype", "sqlalchemy", - "uta-align>=0.3.0a4", + "uta-align>=0.3", ] [project.optional-dependencies] diff --git a/src/uta/loading.py b/src/uta/loading.py index 04c61e7..7d1c310 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -13,12 +13,11 @@ from bioutils.digests import seq_md5 from bioutils.sequences import reverse_complement from sqlalchemy.exc import IntegrityError -from sqlalchemy import text from sqlalchemy.orm.exc import NoResultFound from sqlalchemy import text import psycopg2.extras import six -import uta_align as utaa +from uta_align.align.algorithms import cigar_alignment, needleman_wunsch_gotoh_align from uta.lru_cache import lru_cache @@ -48,10 +47,10 @@ def _get_cursor(con): return cur def align(s1, s2): - score, cigar = utaa.align.algorithms.needleman_wunsch_gotoh_align(s1.encode("ascii"), - s2.encode("ascii"), - extended_cigar=True) - tx_aseq, alt_aseq = utaa.algorithms.cigar_alignment( + score, cigar = needleman_wunsch_gotoh_align(s1.encode("ascii"), + s2.encode("ascii"), + extended_cigar=True) + tx_aseq, alt_aseq = cigar_alignment( tx_seq, alt_seq, cigar, hide_match=False) return tx_aseq.decode("ascii"), alt_aseq.decode("ascii"), cigar.to_string().decode("ascii") From 550b110a406b44aafac62400a58674a85f0757bf Mon Sep 17 00:00:00 2001 From: nvta1209 <162694616+nvta1209@users.noreply.github.com> Date: Fri, 29 Mar 2024 12:38:54 -0700 Subject: [PATCH 24/54] feat(IPVC-2277): handle gbff files that have no exon features (#16) --- pyproject.toml | 6 +- sbin/ncbi-parse-gbff | 44 +--- src/uta/parsers/seqrecord.py | 69 +++++ tests/data/rna.NM_001396027.gbff | 67 +++++ tests/data/rna.NM_001996.gbff | 393 ++++++++++++++++++++++++++++ tests/data/rna.NR_173080.gbff | 61 +++++ tests/data/rna.NR_173148.gbff | 57 ++++ tests/test_uta_parsers_seqrecord.py | 99 +++++++ 8 files changed, 753 insertions(+), 43 deletions(-) create mode 100644 src/uta/parsers/seqrecord.py create mode 100644 tests/data/rna.NM_001396027.gbff create mode 100644 tests/data/rna.NM_001996.gbff create mode 100644 tests/data/rna.NR_173080.gbff create mode 100644 tests/data/rna.NR_173148.gbff create mode 100644 tests/test_uta_parsers_seqrecord.py diff --git a/pyproject.toml b/pyproject.toml index 357be5a..bdb0042 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -57,7 +57,11 @@ dependencies = [ ] [project.optional-dependencies] -test = ["coverage", "testing.postgresql"] +test = [ + "coverage", + "parameterized", + "testing.postgresql", +] [project.urls] # Optional "Homepage" = "https://github.com/biocommons/uta" diff --git a/sbin/ncbi-parse-gbff b/sbin/ncbi-parse-gbff index e3d7d13..ba76e2b 100755 --- a/sbin/ncbi-parse-gbff +++ b/sbin/ncbi-parse-gbff @@ -35,10 +35,11 @@ import re import sys import Bio.SeqIO -import Bio.SeqRecord from bioutils.digests import seq_md5 from uta.formats.txinfo import TxInfo, TxInfoWriter +from uta.parsers.seqrecord import SeqRecordFacade + origin = "NCBI" @@ -57,47 +58,6 @@ def parse_args(argv): return opts -class SeqRecordFacade(Bio.SeqRecord.SeqRecord): - - def __init__(self, seqrecord): - self._sr = seqrecord - - @property - def id(self): - return self._sr.id - - @property - def gene_symbol(self): - genes = [f for f in self._sr.features if f.type == "gene"][ - 0].qualifiers["gene"] - assert len(genes) == 1 - return genes[0] - - @property - def gene_id(self): - # db_xref="GeneID:1234" - db_xrefs = [f for f in self._sr.features if f.type == "gene"][ - 0].qualifiers["db_xref"] - gene_ids = [x.partition(":")[2] for x in db_xrefs if x.startswith("GeneID:")] - assert len(gene_ids) == 1 - return gene_ids[0] - - @property - def cds_se_i(self): - try: - cds = [f for f in self._sr.features if f.type == "CDS"][0] - except IndexError: - return None - return (cds.location.start.real, cds.location.end.real) - - @property - def exons_se_i(self): - # ,"misc_feature"]] - exons = [f for f in self._sr.features if f.type in ["exon"]] - se = [(f.location.start.real, f.location.end.real) for f in exons] - return se - - def gbff_filter(it): """pre-filter genbank file stream for records that match a specific LOCUS pattern""" delim = "//" diff --git a/src/uta/parsers/seqrecord.py b/src/uta/parsers/seqrecord.py new file mode 100644 index 0000000..f8bf57e --- /dev/null +++ b/src/uta/parsers/seqrecord.py @@ -0,0 +1,69 @@ +from collections import defaultdict +from functools import cached_property +from typing import Union + +import Bio.SeqRecord +from Bio.SeqFeature import SeqFeature + + +class SeqRecordFeatureError(Exception): + """Raised when SeqRecord does not have the expected features.""" + + +class SeqRecordFacade: + + def __init__(self, seqrecord: Bio.SeqRecord.SeqRecord): + self._sr = seqrecord + + @cached_property + def features_by_type(self) -> dict[str, list]: + result = defaultdict(list) + for feat in self._sr.features: + result[feat.type].append(feat) + self.validate_features_by_type(result) + return result + + @staticmethod + def validate_features_by_type(features: dict[str, list]) -> None: + """Raise exceptions if feature mapping is invalid.""" + if "CDS" in features and len(features["CDS"]) > 1: + raise SeqRecordFeatureError("Expected one `CDS` feature at most") + if "gene" not in features or len(features["gene"]) != 1: + raise SeqRecordFeatureError("Expected exactly one `gene` feature") + + @cached_property + def gene_feature(self) -> SeqFeature: + """Returns the gene feature, which is assumed to exist for all transcripts. """ + return self.features_by_type.get("gene")[0] + + @property + def id(self): + return self._sr.id + + @property + def gene_symbol(self): + return self.gene_feature.qualifiers["gene"][0] + + @property + def gene_id(self): + # db_xref="GeneID:1234" + db_xrefs = self.gene_feature.qualifiers["db_xref"] + gene_ids = [x.partition(":")[2] for x in db_xrefs if x.startswith("GeneID:")] + assert len(gene_ids) == 1 + return gene_ids[0] + + @property + def cds_se_i(self): + if self.features_by_type.get("CDS"): + cds_feature = self.features_by_type.get("CDS")[0] + return (cds_feature.location.start.real, cds_feature.location.end.real) + else: + return None + + @property + def exons_se_i(self): + if "exon" in self.features_by_type: + exons = self.features_by_type["exon"] + else: + exons = [self.gene_feature] + return [(f.location.start.real, f.location.end.real) for f in exons] diff --git a/tests/data/rna.NM_001396027.gbff b/tests/data/rna.NM_001396027.gbff new file mode 100644 index 0000000..da202f2 --- /dev/null +++ b/tests/data/rna.NM_001396027.gbff @@ -0,0 +1,67 @@ +LOCUS NM_001396027 696 bp mRNA linear PRI 16-APR-2022 +DEFINITION Homo sapiens family with sequence similarity 246 member C + (gene/pseudogene) (FAM246C), mRNA. +ACCESSION NM_001396027 +VERSION NM_001396027.1 +KEYWORDS RefSeq; RefSeq Select. +SOURCE Homo sapiens (human) + ORGANISM Homo sapiens + Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; + Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; + Catarrhini; Hominidae; Homo. +COMMENT VALIDATED REFSEQ: This record has undergone validation or + preliminary review. The reference sequence was derived from + CP068256.2. + + Transcript Variant: This transcript contains two, common, single + nucleotide deletions relative to the GRCh38 reference assembly. + This transcript encodes a protein of 231 aa. + + ##RefSeq-Attributes-START## + polymorphic pseudogene :: based on alignments, homology + RefSeq Select criteria :: based on single protein-coding transcript + ##RefSeq-Attributes-END## +PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP + 1-696 CP068256.2 19404891-19405586 +FEATURES Location/Qualifiers + source 1..696 + /organism="Homo sapiens" + /mol_type="mRNA" + /db_xref="taxon:9606" + /chromosome="22" + /map="22q11.21" + gene 1..696 + /gene="FAM246C" + /note="family with sequence similarity 246 member C + (gene/pseudogene)" + /db_xref="GeneID:117134596" + /db_xref="HGNC:HGNC:54842" + CDS 1..696 + /gene="FAM246C" + /codon_start=1 + /product="protein FAM246C" + /protein_id="NP_001382956.1" + /db_xref="GeneID:117134596" + /db_xref="HGNC:HGNC:54842" + /translation="MAESGRPWAQARSAYRASEVLRRGTGRRRDPGPQSNGPGQEDAR + APGRMARLRGQLRAEAASRSEVPRLLKLVERAGAGAAGAGERTGAHSRGSVCSVCGEP + RGGATYPAGVLEVSERRLQEGLAAVREELGAGIEALRAELRAELDALRALLPPPPSPP + ARREPRAVPRAAPRGPTLPRTLGTVSALVAASRPADDAPDGPAECGAHRAPARKNHKK + MPVPPGAPQGGGD" + exon 1..696 + /gene="FAM246C" + /inference="alignment:Splign:2.1.0" +ORIGIN + 1 atggcggagt ccggccgccc gtgggcccag gcgcgtagtg cgtacagagc cagcgaggtg + 61 ctgcggcgcg gcacgggccg ccggcgggat ccggggccgc aatccaatgg gccgggccag + 121 gaagacgccc gagccccggg ccggatggct cgcctgcgcg gccagctccg ggccgaagcg + 181 gcttcgcggt ccgaggtgcc gcggctgctg aagctggtgg agcgtgcggg ggccggggcg + 241 gcgggcgcgg gcgagaggac cggcgcgcac agccgcggct ccgtgtgctc ggtatgcggg + 301 gagccccgcg gcggggccac ctacccggcg ggggtcctgg aggtgagcga gcggcggctg + 361 caggagggcc tggcggcagt gcgcgaggag ctgggcgccg ggattgaggc gctgcgcgcg + 421 gagcttcgag cggagctgga tgccctgcgc gcgctgctgc cgccgccgcc gtccccgcct + 481 gcccgccgcg agccccgcgc cgtcccccgc gccgcgcccc gcggcccgac cctgccgcgg + 541 acgctcggca ccgtgagcgc cctggtcgcc gcctccaggc ccgcagacga cgccccggac + 601 ggcccagcag aatgcggagc gcaccgagcc ccggccagga agaaccacaa gaagatgcca + 661 gtgccgcctg gggccccgca aggtggcggg gactga +// diff --git a/tests/data/rna.NM_001996.gbff b/tests/data/rna.NM_001996.gbff new file mode 100644 index 0000000..79ff577 --- /dev/null +++ b/tests/data/rna.NM_001996.gbff @@ -0,0 +1,393 @@ +LOCUS NM_001996 2251 bp mRNA linear PRI 18-APR-2022 +DEFINITION Homo sapiens fibulin 1 (FBLN1), transcript variant C, mRNA. +ACCESSION NM_001996 +VERSION NM_001996.4 +KEYWORDS RefSeq. +SOURCE Homo sapiens (human) + ORGANISM Homo sapiens + Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; + Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; + Catarrhini; Hominidae; Homo. +REFERENCE 1 (bases 1 to 2251) + AUTHORS Yang F, Shi J, Zhang L, Wang H and Li Y. + TITLE Role of fibulin-1 gene promoter methylation in the carcinogenesis + and development of tongue squamous cell carcinoma + JOURNAL Oral Surg Oral Med Oral Pathol Oral Radiol 133 (4), 432-440 (2022) + PUBMED 35153187 + REMARK GeneRIF: Role of fibulin-1 gene promoter methylation in the + carcinogenesis and development of tongue squamous cell carcinoma. +REFERENCE 2 (bases 1 to 2251) + AUTHORS Wan Y, Song Y, Chen J, Kong J, Gu C, Huang J and Zuo L. + TITLE Upregulated Fibulin-1 Increased Endometrial Stromal Cell Viability + and Migration by Repressing EFEMP1-Dependent Ferroptosis in + Endometriosis + JOURNAL Biomed Res Int 2022, 4809415 (2022) + PUBMED 35127942 + REMARK GeneRIF: Upregulated Fibulin-1 Increased Endometrial Stromal Cell + Viability and Migration by Repressing EFEMP1-Dependent Ferroptosis + in Endometriosis. + Publication Status: Online-Only +REFERENCE 3 (bases 1 to 2251) + AUTHORS Xu G, Geng X, Yang F and Zhang H. + TITLE FBLN1 promotes chondrocyte proliferation by increasing + phosphorylation of Smad2 + JOURNAL J Orthop Sci 27 (1), 242-248 (2022) + PUBMED 33610427 + REMARK GeneRIF: FBLN1 promotes chondrocyte proliferation by increasing + phosphorylation of Smad2. +REFERENCE 4 (bases 1 to 2251) + AUTHORS Ustunyurt E, Dundar B, Simsek D and Temur M. + TITLE Act of fibulin-1 in preeclamptic patients: can it be a predictive + marker? + JOURNAL J Matern Fetal Neonatal Med 34 (22), 3775-3781 (2021) + PUBMED 34238097 + REMARK GeneRIF: Act of fibulin-1 in preeclamptic patients: can it be a + predictive marker? +REFERENCE 5 (bases 1 to 2251) + AUTHORS Liu XT, Liu TT, Wu MY, Chen QX, Zhuang JX and Wang Q. + TITLE Identifying FBLN1 (Gene ID: 2192) as a Potential Melanoma Biomarker + for Melanoma based on an Analysis of microRNA Expression Profiles + in the GEO and TCGA Databases + JOURNAL Genet Test Mol Biomarkers 25 (1), 68-78 (2021) + PUBMED 33470885 + REMARK GeneRIF: Identifying FBLN1 (Gene ID: 2192) as a Potential Melanoma + Biomarker for Melanoma based on an Analysis of microRNA Expression + Profiles in the GEO and TCGA Databases. +REFERENCE 6 (bases 1 to 2251) + AUTHORS Sasaki T, Gohring W, Pan TC, Chu ML and Timpl R. + TITLE Binding of mouse and human fibulin-2 to extracellular matrix + ligands + JOURNAL J Mol Biol 254 (5), 892-899 (1995) + PUBMED 7500359 +REFERENCE 7 (bases 1 to 2251) + AUTHORS Roark EF, Keene DR, Haudenschild CC, Godyna S, Little CD and + Argraves WS. + TITLE The association of human fibulin-1 with elastic fibers: an + immunohistological, ultrastructural, and RNA study + JOURNAL J Histochem Cytochem 43 (4), 401-411 (1995) + PUBMED 7534784 +REFERENCE 8 (bases 1 to 2251) + AUTHORS Balbona K, Tran H, Godyna S, Ingham KC, Strickland DK and Argraves + WS. + TITLE Fibulin binds to itself and to the carboxyl-terminal + heparin-binding region of fibronectin + JOURNAL J Biol Chem 267 (28), 20120-20125 (1992) + PUBMED 1400330 +REFERENCE 9 (bases 1 to 2251) + AUTHORS Argraves WS, Tran H, Burgess WH and Dickerson K. + TITLE Fibulin is an extracellular matrix and plasma glycoprotein with + repeated domain structure + JOURNAL J Cell Biol 111 (6 Pt 2), 3155-3164 (1990) + PUBMED 2269669 +REFERENCE 10 (bases 1 to 2251) + AUTHORS Argraves WS, Dickerson K, Burgess WH and Ruoslahti E. + TITLE Fibulin, a novel protein that interacts with the fibronectin + receptor beta subunit cytoplasmic domain + JOURNAL Cell 58 (4), 623-629 (1989) + PUBMED 2527614 +COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The + reference sequence was derived from AL021391.3, Z98047.1 and + Z95331.2. + + On May 31, 2019 this sequence version replaced NM_001996.3. + + Summary: Fibulin 1 is a secreted glycoprotein that becomes + incorporated into a fibrillar extracellular matrix. Calcium-binding + is apparently required to mediate its binding to laminin and + nidogen. It mediates platelet adhesion via binding fibrinogen. Four + splice variants which differ in the 3' end have been identified. + Each variant encodes a different isoform, but no functional + distinctions have been identified among the four variants. + [provided by RefSeq, Jul 2008]. + + Transcript Variant: This variant (C) has an alternate 3' sequence, + as compared to variant D. The encoded isoform C is shorter and has + a distinct C-terminus, as compared to isoform D. + + Publication Note: This RefSeq record includes a subset of the + publications that are available for this gene. Please see the Gene + record to access additional publications. + + ##Evidence-Data-START## + Transcript exon combination :: SRR7346977.956579.1, + SRR7346977.1110405.1 [ECO:0000332] + RNAseq introns :: single sample supports all introns + SAMEA1965299, SAMEA1966682 + [ECO:0000348] + ##Evidence-Data-END## + COMPLETENESS: complete on the 3' end. +PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP + 1-182 AL021391.3 135806-135987 + 183-288 Z98047.1 13577-13682 + 289-424 Z98047.1 20438-20573 + 425-587 Z98047.1 22742-22904 + 588-647 Z98047.1 26160-26219 + 648-749 Z98047.1 27958-28059 + 750-887 Z98047.1 28656-28793 + 888-1025 Z98047.1 30095-30232 + 1026-1169 Z98047.1 36124-36267 + 1170-1298 Z98047.1 37050-37178 + 1299-1424 Z98047.1 38296-38421 + 1425-1544 Z98047.1 41980-42099 + 1545-1676 Z98047.1 43508-43639 + 1677-1800 Z98047.1 45387-45510 + 1801-2251 Z95331.2 10369-10819 +FEATURES Location/Qualifiers + source 1..2251 + /organism="Homo sapiens" + /mol_type="mRNA" + /db_xref="taxon:9606" + /chromosome="22" + /map="22q13.31" + gene 1..2251 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="fibulin 1" + /db_xref="GeneID:2192" + /db_xref="HGNC:HGNC:3600" + /db_xref="MIM:135820" + exon 1..182 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + CDS 104..2155 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="isoform C precursor is encoded by transcript + variant C" + /codon_start=1 + /product="fibulin-1 isoform C precursor" + /protein_id="NP_001987.3" + /db_xref="CCDS:CCDS14069.1" + /db_xref="GeneID:2192" + /db_xref="HGNC:HGNC:3600" + /db_xref="MIM:135820" + /translation="MERAAPSRRVPLPLLLLGGLALLAAGVDADVLLEACCADGHRMA + THQKDCSLPYATESKECRMVQEQCCHSQLEELHCATGISLANEQDRCATPHGDNASLE + ATFVKRCCHCCLLGRAAQAQGQSCEYSLMVGYQCGQVFQACCVKSQETGDLDVGGLQE + TDKIIEVEEEQEDPYLNDRCRGGGPCKQQCRDTGDEVVCSCFVGYQLLSDGVSCEDVN + ECITGSHSCRLGESCINTVGSFRCQRDSSCGTGYELTEDNSCKDIDECESGIHNCLPD + FICQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPIGHTCINTEGSYTC + QKNVPNCGRGYHLNEEGTRCVDVDECAPPAEPCGKGHRCVNSPGSFRCECKTGYYFDG + ISRMCVDVNECQRYPGRLCGHKCENTLGSYLCSCSVGFRLSVDGRSCEDINECSSSPC + SQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALPTGGHICSYRCINIPGSFQCS + CPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGGFRCLAFECPENYRRSAAT + RCERLPCHENRECSKLPLRITYYHLSFPTNIQAPAVVFRMGPSSAVPGDSMQLAITGG + NEEGFFTTRKVSPHSGVVALTKPVPEPRDLLLTVKMDLSRHGTVSSFVAKLFIFVSAE + L" + sig_peptide 104..190 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="COORDINATES: ab initio prediction:SignalP:4.0" + mat_peptide 191..2152 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /product="fibulin-1 isoform C" + misc_feature 191..340 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Anaphylatoxin homologous domain; C3a, C4a and C5a + anaphylatoxins are protein fragments generated + enzymatically in serum during activation of complement + molecules C3, C4, and C5. They induce smooth muscle + contraction. These fragments are homologous to...; Region: + ANATO; cd00017" + /db_xref="CDD:237984" + misc_feature 392..559 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Anaphylatoxin homologous domain; C3a, C4a and C5a + anaphylatoxins are protein fragments generated + enzymatically in serum during activation of complement + molecules C3, C4, and C5. They induce smooth muscle + contraction. These fragments are homologous to...; Region: + ANATO; cd00017" + /db_xref="CDD:237984" + misc_feature 698..760 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Complement Clr-like EGF-like; Region: cEGF; + pfam12662" + /db_xref="CDD:432704" + misc_feature 749..832 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Calcium-binding EGF domain; Region: EGF_CA; + pfam07645" + /db_xref="CDD:429571" + misc_feature 887..994 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Calcium-binding EGF domain; Region: EGF_CA; + pfam07645" + /db_xref="CDD:429571" + misc_feature order(887..889,896..898,944..946) + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Ca2+ binding site [ion binding]; other site" + /db_xref="CDD:238011" + misc_feature 1025..1168 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Calcium-binding EGF-like domain; Region: EGF_CA; + smart00179" + /db_xref="CDD:214542" + misc_feature order(1025..1027,1034..1036,1082..1084) + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Ca2+ binding site [ion binding]; other site" + /db_xref="CDD:238011" + misc_feature 1169..1270 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Calcium-binding EGF-like domain; Region: EGF_CA; + smart00179" + /db_xref="CDD:214542" + misc_feature order(1169..1171,1178..1180,1226..1228) + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Ca2+ binding site [ion binding]; other site" + /db_xref="CDD:238011" + misc_feature order(1298..1300,1307..1309,1352..1354) + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Ca2+ binding site [ion binding]; other site" + /db_xref="CDD:238011" + misc_feature 1364..1435 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Complement Clr-like EGF-like; Region: cEGF; + pfam12662" + /db_xref="CDD:432704" + misc_feature 1481..1555 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Complement Clr-like EGF-like; Region: cEGF; + pfam12662" + /db_xref="CDD:432704" + misc_feature 1544..1636 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Calcium-binding EGF-like domain, present in a large + number of membrane-bound and extracellular (mostly animal) + proteins. Many of these proteins require calcium for their + biological function and calcium-binding sites have been + found to be located at the...; Region: EGF_CA; cl21504" + /db_xref="CDD:451279" + misc_feature 1613..1687 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Complement Clr-like EGF-like; Region: cEGF; + pfam12662" + /db_xref="CDD:432704" + misc_feature 1676..1780 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="Calcium-binding EGF domain; Region: EGF_CA; + pfam07645" + /db_xref="CDD:429571" + exon 183..288 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 289..424 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 425..587 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 588..647 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 648..749 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 750..887 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 888..1025 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 1026..1169 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 1170..1298 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 1299..1424 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 1425..1544 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 1545..1676 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 1677..1800 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + exon 1801..2251 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /inference="alignment:Splign:2.1.0" + regulatory 2220..2225 + /regulatory_class="polyA_signal_sequence" + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="hexamer: AATAAA" + polyA_site 2251 + /gene="FBLN1" + /gene_synonym="FBLN; FIBL1" + /note="major polyA site" +ORIGIN + 1 gttggctgcc gaggctcggc cggagcgtgg agcccgcgcc gctgccccag gaccgcgccc + 61 gcgcctttgt ccgccgccgc ccaccgcccg tcgcccgccg cccatggagc gcgccgcgcc + 121 gtcgcgccgg gtcccgcttc cgctgctgct gctcggcggc cttgcgctgc tggcggccgg + 181 agtggacgcg gatgtcctcc tggaggcctg ctgtgcggac ggacaccgga tggccactca + 241 tcagaaggac tgctcgctgc catatgctac ggaatccaaa gaatgcagga tggtgcagga + 301 gcagtgctgc cacagccagc tggaggagct gcactgtgcc acgggcatca gcctggccaa + 361 cgagcaggac cgctgtgcca cgccccacgg tgacaacgcc agcctggagg ccacatttgt + 421 gaagaggtgc tgccattgct gtctgctggg gagggcggcc caggcccagg gccagagctg + 481 cgagtacagc ctcatggttg gctaccagtg tggacaggtc ttccaggcat gctgtgtcaa + 541 gagccaggag accggagatt tggatgtcgg gggcctccaa gaaacggata agatcattga + 601 ggttgaggag gaacaagagg acccatatct gaatgaccgc tgccgaggag gcgggccctg + 661 caagcagcag tgccgagaca cgggtgacga ggtggtctgc tcctgcttcg tgggctacca + 721 gctgctgtct gatggtgtct cctgtgaaga tgtcaatgaa tgcatcacgg gcagccacag + 781 ctgccggctt ggagaatcct gcatcaacac agtgggctct ttccgctgcc agcgggacag + 841 cagctgcggg actggctatg agctcacaga ggacaatagc tgcaaagata ttgacgagtg + 901 tgagagtggt attcataact gcctccccga ttttatctgt cagaatactc tgggatcctt + 961 ccgctgccga cccaagctac agtgcaagag tggctttata caagatgctc taggcaactg + 1021 tattgatatc aatgagtgtt tgagtatcag tgccccgtgc cctatcgggc atacatgcat + 1081 caacacagag ggctcctaca cgtgccagaa gaacgtgccc aactgtggcc gtggctacca + 1141 tctcaacgag gagggaacgc gctgtgttga tgtggacgag tgcgcgccac ctgctgagcc + 1201 ctgtgggaag ggacatcgct gcgtgaactc tcccggcagt ttccgctgcg aatgcaagac + 1261 gggttactat tttgacggca tcagcaggat gtgtgtcgat gtcaacgagt gccagcgcta + 1321 ccccgggcgc ctgtgtggcc acaagtgcga gaacacgctg ggctcctacc tctgcagctg + 1381 ttccgtgggc ttccggctct ctgtggatgg caggtcatgt gaagacatca atgagtgcag + 1441 cagcagcccc tgtagccagg agtgtgccaa cgtctacggc tcctaccagt gttactgccg + 1501 gcgaggctac cagctcagcg atgtggatgg agtcacctgt gaagacatcg acgagtgcgc + 1561 cctgcccacc gggggccaca tctgctccta ccgctgcatc aacatccctg gaagcttcca + 1621 gtgcagctgc ccctcgtctg gctacaggct ggcccccaat ggccgcaact gccaagacat + 1681 tgatgagtgt gtgactggca tccacaactg ctccatcaac gagacctgct tcaacatcca + 1741 gggcggcttc cgctgcctgg ccttcgagtg ccctgagaac taccgccgct ccgcagccac + 1801 ccgctgtgag cgcttgcctt gccatgagaa tcgggagtgc tccaagctgc ctctgagaat + 1861 aacctactac cacctctctt tccccaccaa catccaagcg cccgcggtgg ttttccgcat + 1921 gggcccctcc agtgctgtcc ccggggacag catgcagctg gccatcaccg gcggcaatga + 1981 ggagggcttt ttcaccaccc ggaaggtgag cccccacagt ggggtggtgg ccctcaccaa + 2041 gcctgtcccc gagcccaggg acttgctcct gaccgtcaag atggatctct ctcgccacgg + 2101 caccgtcagc tcctttgtgg ccaagctttt catctttgtg tctgcagagc tctgagcact + 2161 cgcttcgcgt cgcggggtct ccctcctgtt gctttcctaa ccctgccctc cggggcgtta + 2221 ataaagtctt agcaagcgtc ccacacagtg a +// diff --git a/tests/data/rna.NR_173080.gbff b/tests/data/rna.NR_173080.gbff new file mode 100644 index 0000000..f6b2486 --- /dev/null +++ b/tests/data/rna.NR_173080.gbff @@ -0,0 +1,61 @@ +LOCUS NR_173080 1073 bp RNA linear PRI 20-JUL-2023 +DEFINITION Homo sapiens uncharacterized LOC122455341 (LOC122455341), + transcript variant 1, long non-coding RNA. +ACCESSION NR_173080 +VERSION NR_173080.1 +KEYWORDS RefSeq. +SOURCE Homo sapiens (human) + ORGANISM Homo sapiens + Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; + Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; + Catarrhini; Hominidae; Homo. +COMMENT VALIDATED REFSEQ: This record has undergone validation or + preliminary review. The reference sequence was derived from + AC007326.28. + + ##Evidence-Data-START## + RNAseq introns :: single sample supports all introns SAMEA2159764 + [ECO:0000348] + ##Evidence-Data-END## + COMPLETENESS: full length. +PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP + 1-93 AC007326.28 35576-35668 + 94-170 AC007326.28 37874-37950 + 171-1073 AC007326.28 44992-45894 +FEATURES Location/Qualifiers + source 1..1073 + /organism="Homo sapiens" + /mol_type="transcribed RNA" + /db_xref="taxon:9606" + /chromosome="22" + /map="22q11.21" + gene 1..1073 + /gene="LOC122455341" + /note="uncharacterized LOC122455341" + /db_xref="GeneID:122455341" + ncRNA 1..1073 + /ncRNA_class="lncRNA" + /gene="LOC122455341" + /product="uncharacterized LOC122455341, transcript variant + 1" + /db_xref="GeneID:122455341" +ORIGIN + 1 acaagttgta tggttcgttt tatatgaaga gttcagaata gacaaatcga tagagacaga + 61 agtcacacga agacaagccc atagtggagc ctgggtgaag gtacgctcga gcgtggtcat + 121 tgaggacaag tcgacgagag atcccgagta cgtctacagt cagccttacg tctgcaggtg + 181 tacccaacag ctccgaagag acagcgacca tcgagaacgg gccatgatga cgatggcggt + 241 tttgtcgaaa agaaaagggg gaaatgtggg gaaaagcaag agagatcaga ttgttactgt + 301 gtctgtgtag aaagaagtag acataggaga ctccattttg ttatgtgcta agaaaaattc + 361 ttctgccttg agattctgtt aatctataac cttaccccca accccgtgct ctctgaaacg + 421 tgtgctgtgt caactcagag ttaaatggat taagggcggt gcaggatgtg ctttgttaaa + 481 cagatgcttg aaggcagcat gctccttaag agtcatcacc actccctaat ctcaagtacc + 541 cagggacaca aaaactgcgg aaggccgcag ggacctctgc ctaggaaagc caggtattgt + 601 ccaaggtttc tccccatgtg atagtctgaa atatggcctc gtgggaaggg aaagacctga + 661 ccgtccccca gcccgacacc aagggtctgt gctgaggagg attagtaaaa gaggaaggaa + 721 tgcctcttgc agttgagaca agaggaaggc atctgtctcc tgcctgtccc tgggcaatgg + 781 aatgtctcgg tataaaaccc gattgtatgc tccatctact gagataggga aaaaccgcct + 841 tagggctgga ggtgggacct gcgggcagca atactgcttt gtaaagcatt gagatgttta + 901 tgtgtatgca tatctaaaag cacagcactt aatcctttac attgtctatg atgcaaagac + 961 ctttgttcac gtgtttgtct gctgaccctc tccccacaat tgtcttgtga ccctgacaca + 1021 tccccctctt cgagaaacac ccacaagtga tgaataaata ctaagggaac tca +// diff --git a/tests/data/rna.NR_173148.gbff b/tests/data/rna.NR_173148.gbff new file mode 100644 index 0000000..91312ca --- /dev/null +++ b/tests/data/rna.NR_173148.gbff @@ -0,0 +1,57 @@ +LOCUS NR_173148 698 bp RNA linear PRI 17-SEP-2021 +DEFINITION Homo sapiens family with sequence similarity 246 member C + (gene/pseudogene) (FAM246C), non-coding RNA. +ACCESSION NR_173148 +VERSION NR_173148.1 +KEYWORDS RefSeq. +SOURCE Homo sapiens (human) + ORGANISM Homo sapiens + Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; + Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; + Catarrhini; Hominidae; Homo. +COMMENT VALIDATED REFSEQ: This record has undergone validation or + preliminary review. The reference sequence was derived from + AC000095.5. + + Transcript Variant: This transcript matches the GRCh38 reference + assembly. Compared to other members of the FAM246 gene family, it + has a premature stop codon which makes it a non-coding transcript. + + ##RefSeq-Attributes-START## + polymorphic pseudogene :: based on alignments, homology + ##RefSeq-Attributes-END## +PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP + 1-698 AC000095.5 28241-28938 +FEATURES Location/Qualifiers + source 1..698 + /organism="Homo sapiens" + /mol_type="transcribed RNA" + /db_xref="taxon:9606" + /chromosome="22" + /map="22q11.21" + gene 1..698 + /gene="FAM246C" + /note="family with sequence similarity 246 member C + (gene/pseudogene)" + /db_xref="GeneID:117134596" + /db_xref="HGNC:HGNC:54842" + misc_RNA 1..698 + /gene="FAM246C" + /product="family with sequence similarity 246 member C + (gene/pseudogene)" + /db_xref="GeneID:117134596" + /db_xref="HGNC:HGNC:54842" +ORIGIN + 1 atggcggagc ccggccgccc gtgggcccag gcgcgtagtg cgtacagagc cagcgaggtg + 61 ctgcggcgcg gcacgggccg ccggcgggat ccggggccgc aatccaatgg gccgggccag + 121 gaagacgccc gagccccggg ccggatggct cgcctgcgcg gccagctccg ggccgaagcg + 181 gcttcgcggt ccgaggtgcc gcggctgctg aagctggtgg agcgtgcggg ggccggggcg + 241 gccgggcgcg ggcgagagga ccggcgcgca cagccgcggg ctccgtgtgc tcggtatgcg + 301 gggagccccg cggcggggcc acctacccgg cgggggtcct ggaggtgagc gagcggcggc + 361 tgcaggaggg cctggcggca gtgcgcgagg agctgggcgc cgggattgag gcgctgcgcg + 421 cggagcttcg agcggagctg gatgccctgc gcgcgctgct gccgccgccg ccgtccccgc + 481 ctgcccgccg cgagccccgc gccgtccccc gcgccgcgcc ccgcggcccg accctgccgc + 541 ggacgctcgg caccgtgagc gccctggtcg ccgcctccag gcccgcagac gacgccccgg + 601 acggcccagc agaatgcgga gcgcaccgag ccccggccag gaagaaccac aagaagatgc + 661 cagtgccgcc tggggccccg caaggtggcg gggactga +// diff --git a/tests/test_uta_parsers_seqrecord.py b/tests/test_uta_parsers_seqrecord.py new file mode 100644 index 0000000..3b23805 --- /dev/null +++ b/tests/test_uta_parsers_seqrecord.py @@ -0,0 +1,99 @@ +import os +import unittest +from unittest.mock import Mock + +from Bio import SeqIO +from parameterized import param, parameterized + +from uta.parsers.seqrecord import SeqRecordFacade, SeqRecordFeatureError + + +class TestSeqRecordFacade(unittest.TestCase): + test_data_dir = os.path.join(os.path.dirname(__file__), 'data') + + @parameterized.expand([ + param( + 'NM_001396027 - single exon feature', + file_name='rna.NM_001396027.gbff', + expected_id='NM_001396027.1', + expected_gene_symbol='FAM246C', + expected_gene_id='117134596', + expected_cds_se_i=(0, 696), + expected_exons_se_i=[(0, 696)], + ), + param( + 'NM_001396027 - multiple exon features', + file_name='rna.NM_001996.gbff', + expected_id='NM_001996.4', + expected_gene_symbol='FBLN1', + expected_gene_id='2192', + expected_cds_se_i=(103, 2155), + expected_exons_se_i=[ + (0, 182), + (182, 288), + (288, 424), + (424, 587), + (587, 647), + (647, 749), + (749, 887), + (887, 1025), + (1025, 1169), + (1169, 1298), + (1298, 1424), + (1424, 1544), + (1544, 1676), + (1676, 1800), + (1800, 2251), + ], + ), + param( + 'NR_173080 - no exon features, ncRNA', + file_name='rna.NR_173080.gbff', + expected_id='NR_173080.1', + expected_gene_symbol='LOC122455341', + expected_gene_id='122455341', + expected_cds_se_i=None, + expected_exons_se_i=[(0,1073)], + ), + param( + 'NR_173148 - no exon features, misc_RNA', + file_name='rna.NR_173148.gbff', + expected_id='NR_173148.1', + expected_gene_symbol='FAM246C', + expected_gene_id='117134596', + expected_cds_se_i=None, + expected_exons_se_i=[(0,698)], + ), + ]) + def test_seq_record_facade( + self, + test_name, + file_name, + expected_id, + expected_gene_symbol, + expected_gene_id, + expected_cds_se_i, + expected_exons_se_i, + ): + gbff_file = os.path.join(self.test_data_dir, file_name) + seq_record = [sr for sr in SeqIO.parse(gbff_file, 'gb')][0] + self.seq_record_facade = SeqRecordFacade(seq_record) + assert self.seq_record_facade.id == expected_id + assert self.seq_record_facade.gene_symbol == expected_gene_symbol + assert self.seq_record_facade.gene_id == expected_gene_id + assert self.seq_record_facade.cds_se_i == expected_cds_se_i + assert self.seq_record_facade.exons_se_i == expected_exons_se_i + + @parameterized.expand([ + param('no genes', features={}), + param('no genes', features={'gene': []}), + param('more than one gene', features={'gene': [Mock(), Mock()]}), + param('more than one CDS', features={'CDS': [Mock(), Mock()]}), + ]) + def test_validate_features_by_type_invalid(self, test_name, features): + with self.assertRaises(SeqRecordFeatureError): + SeqRecordFacade.validate_features_by_type(features) + + +if __name__ == '__main__': + unittest.main() From e0e4ff39be210013bb3aa6e732cde2d8c02450a3 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Mon, 1 Apr 2024 10:08:59 -0600 Subject: [PATCH 25/54] feat(IPVC-2228): Extract mito gene data from genbank file (#14) --- pyproject.toml | 2 + sbin/ncbi_process_mito.py | 335 +++++++++ tests/data/NC_012920.1.gbff | 1165 +++++++++++++++++++++++++++++++ tests/test_ncbi_process_mito.py | 279 ++++++++ 4 files changed, 1781 insertions(+) create mode 100644 sbin/ncbi_process_mito.py create mode 100644 tests/data/NC_012920.1.gbff create mode 100644 tests/test_ncbi_process_mito.py diff --git a/pyproject.toml b/pyproject.toml index bdb0042..5b6b4a2 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -47,6 +47,8 @@ dependencies = [ "configparser", "docopt", "eutils>=0.3.2", + "importlib_resources", + "more_itertools", "nose", "prettytable", "psycopg2-binary", diff --git a/sbin/ncbi_process_mito.py b/sbin/ncbi_process_mito.py new file mode 100644 index 0000000..2afe071 --- /dev/null +++ b/sbin/ncbi_process_mito.py @@ -0,0 +1,335 @@ +""" +Download mito fasta and gbff file. Use BioPython to parse the features in the Mitochondrial genbank file to get +the attributes of a region of the genome that correspond to genes along with their attributes. Output gene/tx/alignment +details to intermediate file needed to update UTA database and SeqRepo. + + FEATURES Location/Qualifiers + source 1..16569 + /organism="Homo sapiens" + /organelle="mitochondrion" + /mol_type="genomic DNA" + /isolation_source="caucasian" + /db_xref="taxon:9606" + /tissue_type="placenta" + /country="United Kingdom: Great Britain" + /note="this is the rCRS" + D-loop complement(join(16024..16569,1..576)) + gene 577..647 + /gene="TRNF" + /nomenclature="Official Symbol: MT-TF | Name: + mitochondrially encoded tRNA phenylalanine | Provided by: + HGNC:HGNC:7481" + /db_xref="GeneID:4558" + /db_xref="HGNC:HGNC:7481" + /db_xref="MIM:590070" + tRNA 577..647 + /gene="TRNF" + /product="tRNA-Phe" + /note="NAR: 1455" + /anticodon=(pos:611..613,aa:Phe,seq:gaa) + /codon_recognized="UUC" + /db_xref="GeneID:4558" + /db_xref="HGNC:HGNC:7481" + /db_xref="MIM:590070" + gene 648..1601 + /gene="RNR1" + /gene_synonym="MTRNR1" + /nomenclature="Official Symbol: MT-RNR1 | Name: + mitochondrially encoded 12S RNA | Provided by: + HGNC:HGNC:7470" + /db_xref="GeneID:4549" + /db_xref="HGNC:HGNC:7470" + /db_xref="MIM:561000" + rRNA 648..1601 + /gene="RNR1" + /gene_synonym="MTRNR1" + /product="s-rRNA" + /note="12S rRNA; 12S ribosomal RNA" + /db_xref="GeneID:4549" + /db_xref="HGNC:HGNC:7470" + /db_xref="MIM:561000" + ... +""" +import argparse +import dataclasses +import importlib_resources +import logging +import logging.config +from typing import Dict, Optional + +from Bio.Seq import Seq +import Bio.SeqIO +from Bio.SeqFeature import SeqFeature +from Bio.SeqRecord import SeqRecord +from bioutils.digests import seq_md5 +from more_itertools import first, one + +from uta.formats.geneaccessions import GeneAccessions, GeneAccessionsWriter +from uta.formats.seqinfo import SeqInfo, SeqInfoWriter +from uta.formats.txinfo import TxInfo, TxInfoWriter +from uta.formats.exonset import ExonSet, ExonSetWriter +from uta.tools.eutils import download_from_eutils, NcbiFileFormatEnum + + +@dataclasses.dataclass +class MitoGeneData: + gene_id: int + gene_symbol: str + name: str + tx_ac: str + tx_seq: str + tx_start: int + tx_end: int + alt_ac: str + alt_start: int + alt_end: int + strand: str + origin: str = "NCBI" + alignment_method: str = "splign" + transl_table: Optional[str] = None + transl_except: Optional[str] = None + pro_ac: Optional[str] = None + pro_seq: Optional[str] = None + + def exons_se_i(self) -> str: + return f"{self.tx_start},{self.tx_end}" + + def cds_se_i(self) -> str: + return self.exons_se_i() if self.pro_ac else "" + + def alt_exons_se_i(self) -> str: + return f"{self.alt_start},{self.alt_end}" + + +logging_conf_fn = importlib_resources.files("uta").joinpath("etc/logging.conf") +logging.config.fileConfig(logging_conf_fn) +logging.getLogger().setLevel(logging.INFO) +logger = logging.getLogger(__name__) + + +def parse_args(): + parser = argparse.ArgumentParser(description=__doc__) + parser.add_argument("accession", type=str) + parser.add_argument("--output-dir", "-o", default=".", type=str) + return parser.parse_args() + + +def download_mito_files(output_dir: str, accession: str) -> Dict[str, str]: + logger.info(f"downloading files for {accession}") + mt_gb_filepath = f"{output_dir}/{accession}.gbff" + mt_fa_filepath = f"{output_dir}/{accession}.fna" + + logger.info(f"downloading {NcbiFileFormatEnum.GENBANK} file to {mt_gb_filepath}") + download_from_eutils(accession, NcbiFileFormatEnum.GENBANK, mt_gb_filepath) + + logger.info(f"downloading {NcbiFileFormatEnum.FASTA} file to {mt_fa_filepath}") + download_from_eutils(accession, NcbiFileFormatEnum.FASTA, mt_fa_filepath) + + return {"gbff": mt_gb_filepath, "fna": mt_fa_filepath} + + +def parse_db_xrefs(gb_feature: SeqFeature) -> Dict[str, str]: + """ + Example: + Key: db_xref + Value: ['GeneID:4558', 'HGNC:HGNC:7481', 'MIM:590070'] + """ + return { + x.partition(":")[0].strip(): x.partition(":")[2].strip() + for x in gb_feature.qualifiers.get("db_xref", []) + } + + +def parse_nomenclature_value(gb_feature: SeqFeature) -> Dict[str, str]: + """ + Example: + Key: nomenclature + Value: ['Official Symbol: MT-TF | Name: mitochondrially encoded tRNA phenylalanine | Provided by: HGNC:HGNC:7481'] + """ + nomenclature_key = "nomenclature" + nomenclature_results: Dict[str, str] = {} + if nomenclature_key in gb_feature.qualifiers: + nomenclature_list = list( + map( + lambda x: x.strip(), + one(gb_feature.qualifiers[nomenclature_key]).split("|"), + ) + ) + nomenclature_results = { + x.partition(":")[0].strip(): x.partition(":")[2].strip() + for x in nomenclature_list + } + + return nomenclature_results + + +def get_mito_genes(gbff_filepath: str): + logger.info(f"processing NCBI GBFF file from {gbff_filepath}") + with open(gbff_filepath) as fh: + for record in Bio.SeqIO.parse(fh, "gb"): + for feature in record.features: + xrefs = parse_db_xrefs(feature) + + feature_start, feature_end = ( + feature.location.start, + feature.location.end, + ) + + # dependent on feature type, process data and output if appropriate + if feature.type == "gene": + # assert subsequent features represent the same location + assert feature_start == feature.location.start + assert feature_end == feature.location.end + # for gene feature do not yield anything, just set gene level attributes + gene_id = int(xrefs["GeneID"]) + nomenclature = parse_nomenclature_value(feature) + hgnc = nomenclature["Official Symbol"] + name = nomenclature["Name"] + + elif feature.type in ("tRNA", "rRNA", "CDS"): + # assert subsequent features represent the same location and gene + assert int(xrefs["GeneID"]) == gene_id + assert feature_start == feature.location.start + assert feature_end == feature.location.end + # if feature type not CDS, set defaults + pro_ac = None + pro_seq = None + transl_table = None + transl_except = None + + # retrieve sequence, and reverse compliment if on reverse strand + ac = f"{record.id}_{feature.location.start:05}_{feature.location.end:05}" + feature_seq = record.seq[feature_start:feature_end] + strand = "+" + if feature.location.strand == -1: + strand = "-" + feature_seq = feature_seq.reverse_complement() + + if feature.type == "CDS": + # override defaults for CDS features + pro_ac = one(feature.qualifiers["protein_id"]) + pro_seq = str(one(feature.qualifiers["translation"])) + transl_table = one(feature.qualifiers["transl_table"]) + if "transl_except" in feature.qualifiers: + transl_except = one(feature.qualifiers["transl_except"]) + + # yield gene data + yield MitoGeneData( + gene_id=gene_id, + gene_symbol=hgnc, + name=name, + tx_ac=ac, + tx_seq=str(feature_seq), + tx_start=0, + tx_end=feature.location.end - feature.location.start, + alt_ac=record.id, + alt_start=feature_start, + alt_end=feature_end, + strand=strand, + transl_table=transl_table, + transl_except=transl_except, + pro_ac=pro_ac, + pro_seq=pro_seq, + ) + + +def main(ncbi_accession: str, output_dir: str): + # get input files + input_files = download_mito_files(output_dir=output_dir, accession=ncbi_accession) + + # extract Mitochondrial gene information + mito_genes = [mg for mf in input_files.values() for mg in get_mito_genes(mf)] + logger.info(f"found {len(mito_genes)} genes from parsing {input_files['gbff']}") + + # write gene accessions + with open(f"{output_dir}/{ncbi_accession}.assocacs", "w") as o_file: + gaw = GeneAccessionsWriter(o_file) + for mg in mito_genes: + if mg.pro_ac is not None: + gaw.write( + GeneAccessions( + mg.gene_symbol, mg.tx_ac, mg.gene_id, mg.pro_ac, mg.origin + ) + ) + + # write sequence information + with open(f"{output_dir}/{ncbi_accession}.seqinfo", "w") as o_file: + siw = SeqInfoWriter(o_file) + for mg in mito_genes: + siw.write( + SeqInfo( + seq_md5(mg.tx_seq), + mg.origin, + mg.tx_ac, + mg.name, + len(mg.tx_seq), + None, + ) + ) + if mg.pro_ac is not None: + siw.write( + SeqInfo( + seq_md5(mg.pro_seq), + mg.origin, + mg.pro_ac, + mg.name, + len(mg.pro_seq), + None, + ) + ) + + # write out transcript sequence fasta files. + with open(f"{output_dir}/{ncbi_accession}.rna.fna", "w") as o_file: + for mg in mito_genes: + record = SeqRecord( + Seq(mg.tx_seq), + id=mg.tx_ac, + description=mg.name, + ) + o_file.write(record.format("fasta")) + + # write out protein sequence fasta files. + with open(f"{output_dir}/{ncbi_accession}.protein.faa", "w") as o_file: + for mg in mito_genes: + if mg.pro_ac is not None: + record = SeqRecord( + Seq(mg.pro_seq), + id=mg.pro_ac, + description=mg.name, + ) + o_file.write(record.format("fasta")) + + # write transcript information + with open(f"{output_dir}/{ncbi_accession}.txinfo", "w") as o_file: + tiw = TxInfoWriter(o_file) + for mg in mito_genes: + tiw.write( + TxInfo( + mg.origin, + mg.tx_ac, + mg.gene_id, + mg.gene_symbol, + mg.cds_se_i(), + mg.exons_se_i(), + ) + ) + + # write exonset + with open(f"{output_dir}/{ncbi_accession}.exonset", "w") as o_file: + esw = ExonSetWriter(o_file) + for mg in mito_genes: + esw.write( + ExonSet( + mg.tx_ac, + mg.alt_ac, + mg.alignment_method, + mg.strand, + mg.alt_exons_se_i(), + ) + ) + + +if __name__ == "__main__": + args = parse_args() + + main(args.accession, args.output_dir) diff --git a/tests/data/NC_012920.1.gbff b/tests/data/NC_012920.1.gbff new file mode 100644 index 0000000..afea7f3 --- /dev/null +++ b/tests/data/NC_012920.1.gbff @@ -0,0 +1,1165 @@ +LOCUS NC_012920 16569 bp DNA circular PRI 03-APR-2023 +DEFINITION Homo sapiens mitochondrion, complete genome. +ACCESSION NC_012920 AC_000021 +VERSION NC_012920.1 +DBLINK BioProject: PRJNA927338 +KEYWORDS RefSeq. +SOURCE mitochondrion Homo sapiens (human) + ORGANISM Homo sapiens + Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; + Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; + Catarrhini; Hominidae; Homo. +REFERENCE 1 (bases 324 to 743) + AUTHORS Andrews,R.M., Kubacka,I., Chinnery,P.F., Lightowlers,R.N., + Turnbull,D.M. and Howell,N. + TITLE Reanalysis and revision of the Cambridge reference sequence for + human mitochondrial DNA + JOURNAL Nat. Genet. 23 (2), 147 (1999) + PUBMED 10508508 +REFERENCE 2 (bases 15888 to 15954) + AUTHORS Anderson,S., Bankier,A.T., Barrell,B.G., de Bruijn,M.H., + Coulson,A.R., Drouin,J., Eperon,I.C., Nierlich,D.P., Roe,B.A., + Sanger,F., Schreier,P.H., Smith,A.J., Staden,R. and Young,I.G. + TITLE Sequence and organization of the human mitochondrial genome + JOURNAL Nature 290 (5806), 457-465 (1981) + PUBMED 7219534 +REFERENCE 3 (bases 1 to 16569) + CONSRTM NCBI Genome Project + TITLE Direct Submission + JOURNAL Submitted (08-JUL-2009) National Center for Biotechnology + Information, NIH, Bethesda, MD 20894, USA +REFERENCE 4 (bases 1 to 16569) + AUTHORS Kogelnik,A.M. and Lott,M.T. + TITLE Direct Submission + JOURNAL Submitted (24-AUG-2006) Mitomap.org, Center for Molecular and + Mitochondrial Medicine and Genetics (MAMMAG) University of + California, University of California, Irvine, Irvine, CA + 92697-3940, USA + REMARK Sequence update by submitter +REFERENCE 5 (bases 1 to 16569) + AUTHORS Kogelnik,A.M. and Lott,M.T. + TITLE Direct Submission + JOURNAL Submitted (18-APR-1997) Center for Molecular Medicine, Emory + University School of Medicine, 1462 Clifton Road, Suite 420, + Atlanta, GA 30322, USA + REMARK sequence updated +COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final + NCBI review. The reference sequence was derived from J01415. + + On Jul 8, 2009 this sequence version replaced AC_000021.2. + This sequence is a corrected version of the HUMMTCG reference + sequence. The original Cambridge reference sequence (CRS) is + preserved as GenBank J01415 gi:337188 [PMID:7219534]. Corrections + have been made and annotated per the re-sequencing of the original + material by Andrews et al [PMID:10508508]. + + This Revised Cambridge Reference Sequence (rCRS) has eighteen + specific corrections or confirmations of the original 1981 sequence + of Anderson et al [PMID:7219534]. Seven nucleotides are confirmed + as rare polymorphisms, maintained as: 263A, 311C-315C, 750A, 1438A, + 4769A, 8860A, and 15326A. Eleven nucleotides are error + corrections: 3107del, 3423T, 4985A, 9559C, 11335C, 13702C, 14199T, + 14272C, 14365C, 14368C, and 14766C. These 11 errors in the + original Cambridge sequence were determined to be either outright + sequencing errors (8 instances) or due to the presence of bovine + DNA (2 instances) or HeLa DNA (1 instance) mixed in with the + original human placental DNA [PMID:10508508]. HISTORICAL + NUCLEOTIDE NUMBERS ARE MAINTAINED by indicating 3107del as 'N'. + A summary table of the reanalysis data is available online at + http://www.mitomap.org/MITOMAP/CambridgeReanalysis + + L-strand is shown. + COMPLETENESS: full length. +FEATURES Location/Qualifiers + source 1..16569 + /organism="Homo sapiens" + /organelle="mitochondrion" + /mol_type="genomic DNA" + /isolation_source="caucasian" + /db_xref="taxon:9606" + /tissue_type="placenta" + /country="United Kingdom: Great Britain" + /note="this is the rCRS" + D-loop complement(join(16024..16569,1..576)) + gene 577..647 + /gene="TRNF" + /nomenclature="Official Symbol: MT-TF | Name: + mitochondrially encoded tRNA phenylalanine | Provided by: + HGNC:HGNC:7481" + /db_xref="GeneID:4558" + /db_xref="HGNC:HGNC:7481" + /db_xref="MIM:590070" + tRNA 577..647 + /gene="TRNF" + /product="tRNA-Phe" + /note="NAR: 1455" + /anticodon=(pos:611..613,aa:Phe,seq:gaa) + /codon_recognized="UUC" + /db_xref="GeneID:4558" + /db_xref="HGNC:HGNC:7481" + /db_xref="MIM:590070" + gene 648..1601 + /gene="RNR1" + /gene_synonym="MTRNR1" + /nomenclature="Official Symbol: MT-RNR1 | Name: + mitochondrially encoded 12S RNA | Provided by: + HGNC:HGNC:7470" + /db_xref="GeneID:4549" + /db_xref="HGNC:HGNC:7470" + /db_xref="MIM:561000" + rRNA 648..1601 + /gene="RNR1" + /gene_synonym="MTRNR1" + /product="s-rRNA" + /note="12S rRNA; 12S ribosomal RNA" + /db_xref="GeneID:4549" + /db_xref="HGNC:HGNC:7470" + /db_xref="MIM:561000" + gene 1602..1670 + /gene="TRNV" + /gene_synonym="MTTV" + /nomenclature="Official Symbol: MT-TV | Name: + mitochondrially encoded tRNA valine | Provided by: + HGNC:HGNC:7500" + /db_xref="GeneID:4577" + /db_xref="HGNC:HGNC:7500" + /db_xref="MIM:590105" + tRNA 1602..1670 + /gene="TRNV" + /gene_synonym="MTTV" + /product="tRNA-Val" + /note="NAR: 2053" + /anticodon=(pos:1633..1635,aa:Val,seq:tac) + /codon_recognized="GUA" + /db_xref="GeneID:4577" + /db_xref="HGNC:HGNC:7500" + /db_xref="MIM:590105" + gene 1671..3229 + /gene="RNR2" + /gene_synonym="MTRNR2" + /nomenclature="Official Symbol: MT-RNR2 | Name: + mitochondrially encoded 16S RNA | Provided by: + HGNC:HGNC:7471" + /db_xref="GeneID:4550" + /db_xref="HGNC:HGNC:7471" + /db_xref="MIM:561010" + rRNA 1671..3229 + /gene="RNR2" + /gene_synonym="MTRNR2" + /product="l-rRNA" + /note="16S ribosomal RNA; 16S rRNA" + /db_xref="GeneID:4550" + /db_xref="HGNC:HGNC:7471" + /db_xref="MIM:561010" + misc_feature 3107 + /note="preserves historical genome annotation numbering" + gene 3230..3304 + /gene="TRNL1" + /gene_synonym="MTTL1" + /nomenclature="Official Symbol: MT-TL1 | Name: + mitochondrially encoded tRNA leucine 1 (UUA/G) | Provided + by: HGNC:HGNC:7490" + /db_xref="GeneID:4567" + /db_xref="HGNC:HGNC:7490" + /db_xref="MIM:590050" + tRNA 3230..3304 + /gene="TRNL1" + /gene_synonym="MTTL1" + /product="tRNA-Leu" + /note="NAR: 1054" + /anticodon=(pos:3265..3267,aa:Leu,seq:taa) + /codon_recognized="UUR" + /db_xref="GeneID:4567" + /db_xref="HGNC:HGNC:7490" + /db_xref="MIM:590050" + gene 3307..4262 + /gene="ND1" + /gene_synonym="MTND1" + /nomenclature="Official Symbol: MT-ND1 | Name: + mitochondrially encoded NADH dehydrogenase 1 | Provided + by: HGNC:HGNC:7455" + /db_xref="GeneID:4535" + /db_xref="HGNC:HGNC:7455" + /db_xref="MIM:516000" + CDS 3307..4262 + /gene="ND1" + /gene_synonym="MTND1" + /note="NADH dehydrogenase, subunit 1 (complex I); TAA stop + codon is completed by the addition of 3' A residues to the + mRNA" + /codon_start=1 + /transl_except=(pos:4261..4262,aa:TERM) + /transl_table=2 + /product="NADH dehydrogenase subunit 1" + /protein_id="YP_003024026.1" + /db_xref="GeneID:4535" + /db_xref="HGNC:HGNC:7455" + /db_xref="MIM:516000" + /translation="MPMANLLLLIVPILIAMAFLMLTERKILGYMQLRKGPNVVGPYG + LLQPFADAMKLFTKEPLKPATSTITLYITAPTLALTIALLLWTPLPMPNPLVNLNLGL + LFILATSSLAVYSILWSGWASNSNYALIGALRAVAQTISYEVTLAIILLSTLLMSGSF + NLSTLITTQEHLWLLLPSWPLAMMWFISTLAETNRTPFDLAEGESELVSGFNIEYAAG + PFALFFMAEYTNIIMMNTLTTTIFLGTTYDALSPELYTTYFVTKTLLLTSLFLWIRTA + YPRFRYDQLMHLLWKNFLPLTLALLMWYVSMPITISSIPPQT" + gene 4263..4331 + /gene="TRNI" + /gene_synonym="MTTI" + /nomenclature="Official Symbol: MT-TI | Name: + mitochondrially encoded tRNA isoleucine | Provided by: + HGNC:HGNC:7488" + /db_xref="GeneID:4565" + /db_xref="HGNC:HGNC:7488" + /db_xref="MIM:590045" + tRNA 4263..4331 + /gene="TRNI" + /gene_synonym="MTTI" + /product="tRNA-Ile" + /note="NAR: 0997" + /anticodon=(pos:4292..4294,aa:Ile,seq:gat) + /codon_recognized="AUC" + /db_xref="GeneID:4565" + /db_xref="HGNC:HGNC:7488" + /db_xref="MIM:590045" + gene complement(4329..4400) + /gene="TRNQ" + /gene_synonym="MTTQ" + /nomenclature="Official Symbol: MT-TQ | Name: + mitochondrially encoded tRNA glutamine | Provided by: + HGNC:HGNC:7495" + /db_xref="GeneID:4572" + /db_xref="HGNC:HGNC:7495" + /db_xref="MIM:590030" + tRNA complement(4329..4400) + /gene="TRNQ" + /gene_synonym="MTTQ" + /product="tRNA-Gln" + /note="NAR: 0597" + /anticodon=(pos:complement(4365..4367),aa:Gln,seq:ttg) + /codon_recognized="CAA" + /db_xref="GeneID:4572" + /db_xref="HGNC:HGNC:7495" + /db_xref="MIM:590030" + gene 4402..4469 + /gene="TRNM" + /gene_synonym="MTTM" + /nomenclature="Official Symbol: MT-TM | Name: + mitochondrially encoded tRNA methionine | Provided by: + HGNC:HGNC:7492" + /db_xref="GeneID:4569" + /db_xref="HGNC:HGNC:7492" + /db_xref="MIM:590065" + tRNA 4402..4469 + /gene="TRNM" + /gene_synonym="MTTM" + /product="tRNA-Met" + /note="NAR: 1297" + /anticodon=(pos:4432..4434,aa:Met,seq:cat) + /codon_recognized="AUG" + /db_xref="GeneID:4569" + /db_xref="HGNC:HGNC:7492" + /db_xref="MIM:590065" + gene 4470..5511 + /gene="ND2" + /gene_synonym="MTND2" + /nomenclature="Official Symbol: MT-ND2 | Name: + mitochondrially encoded NADH dehydrogenase 2 | Provided + by: HGNC:HGNC:7456" + /db_xref="GeneID:4536" + /db_xref="HGNC:HGNC:7456" + /db_xref="MIM:516001" + CDS 4470..5511 + /gene="ND2" + /gene_synonym="MTND2" + /note="TAA stop codon is completed by the addition of 3' A + residues to the mRNA" + /codon_start=1 + /transl_except=(pos:5511,aa:TERM) + /transl_table=2 + /product="NADH dehydrogenase subunit 2" + /protein_id="YP_003024027.1" + /db_xref="GeneID:4536" + /db_xref="HGNC:HGNC:7456" + /db_xref="MIM:516001" + /translation="MNPLAQPVIYSTIFAGTLITALSSHWFFTWVGLEMNMLAFIPVL + TKKMNPRSTEAAIKYFLTQATASMILLMAILFNNMLSGQWTMTNTTNQYSSLMIMMAM + AMKLGMAPFHFWVPEVTQGTPLTSGLLLLTWQKLAPISIMYQISPSLNVSLLLTLSIL + SIMAGSWGGLNQTQLRKILAYSSITHMGWMMAVLPYNPNMTILNLTIYIILTTTAFLL + LNLNSSTTTLLLSRTWNKLTWLTPLIPSTLLSLGGLPPLTGFLPKWAIIEEFTKNNSL + IIPTIMATITLLNLYFYLRLIYSTSITLLPMSNNVKMKWQFEHTKPTPFLPTLIALTT + LLLPISPFMLMIL" + gene 5512..5579 + /gene="TRNW" + /gene_synonym="MTTW" + /nomenclature="Official Symbol: MT-TW | Name: + mitochondrially encoded tRNA tryptophan | Provided by: + HGNC:HGNC:7501" + /db_xref="GeneID:4578" + /db_xref="HGNC:HGNC:7501" + /db_xref="MIM:590095" + tRNA 5512..5579 + /gene="TRNW" + /gene_synonym="MTTW" + /product="tRNA-Trp" + /note="NAR: 1897" + /anticodon=(pos:5544..5546,aa:Trp,seq:tca) + /codon_recognized="UGA" + /db_xref="GeneID:4578" + /db_xref="HGNC:HGNC:7501" + /db_xref="MIM:590095" + gene complement(5587..5655) + /gene="TRNA" + /gene_synonym="MTTA" + /nomenclature="Official Symbol: MT-TA | Name: + mitochondrially encoded tRNA alanine | Provided by: + HGNC:HGNC:7475" + /db_xref="GeneID:4553" + /db_xref="HGNC:HGNC:7475" + /db_xref="MIM:590000" + tRNA complement(5587..5655) + /gene="TRNA" + /gene_synonym="MTTA" + /product="tRNA-Ala" + /note="NAR: 0097" + /anticodon=(pos:complement(5623..5625),aa:Ala,seq:tgc) + /codon_recognized="GCA" + /db_xref="GeneID:4553" + /db_xref="HGNC:HGNC:7475" + /db_xref="MIM:590000" + gene complement(5657..5729) + /gene="TRNN" + /gene_synonym="MTTN" + /nomenclature="Official Symbol: MT-TN | Name: + mitochondrially encoded tRNA asparagine | Provided by: + HGNC:HGNC:7493" + /db_xref="GeneID:4570" + /db_xref="HGNC:HGNC:7493" + /db_xref="MIM:590010" + tRNA complement(5657..5729) + /gene="TRNN" + /gene_synonym="MTTN" + /product="tRNA-Asn" + /note="NAR: 0297" + /anticodon=(pos:complement(5694..5696),aa:Asn,seq:gtt) + /codon_recognized="AAC" + /db_xref="GeneID:4570" + /db_xref="HGNC:HGNC:7493" + /db_xref="MIM:590010" + gene complement(5761..5826) + /gene="TRNC" + /gene_synonym="MTTC" + /nomenclature="Official Symbol: MT-TC | Name: + mitochondrially encoded tRNA cysteine | Provided by: + HGNC:HGNC:7477" + /db_xref="GeneID:4511" + /db_xref="HGNC:HGNC:7477" + /db_xref="MIM:590020" + tRNA complement(5761..5826) + /gene="TRNC" + /gene_synonym="MTTC" + /product="tRNA-Cys" + /note="NAR: 0497" + /anticodon=(pos:complement(5796..5798),aa:Cys,seq:gca) + /codon_recognized="UGC" + /db_xref="GeneID:4511" + /db_xref="HGNC:HGNC:7477" + /db_xref="MIM:590020" + gene complement(5826..5891) + /gene="TRNY" + /gene_synonym="MTTY" + /nomenclature="Official Symbol: MT-TY | Name: + mitochondrially encoded tRNA tyrosine | Provided by: + HGNC:HGNC:7502" + /db_xref="GeneID:4579" + /db_xref="HGNC:HGNC:7502" + /db_xref="MIM:590100" + tRNA complement(5826..5891) + /gene="TRNY" + /gene_synonym="MTTY" + /product="tRNA-Tyr" + /note="NAR: 1997" + /anticodon=(pos:complement(5860..5862),aa:Tyr,seq:gta) + /codon_recognized="UAC" + /db_xref="GeneID:4579" + /db_xref="HGNC:HGNC:7502" + /db_xref="MIM:590100" + gene 5904..7445 + /gene="COX1" + /gene_synonym="COI; MTCO1" + /nomenclature="Official Symbol: MT-CO1 | Name: + mitochondrially encoded cytochrome c oxidase I | Provided + by: HGNC:HGNC:7419" + /db_xref="GeneID:4512" + /db_xref="HGNC:HGNC:7419" + /db_xref="MIM:516030" + CDS 5904..7445 + /gene="COX1" + /gene_synonym="COI; MTCO1" + /note="cytochrome c oxidase I" + /codon_start=1 + /transl_table=2 + /product="cytochrome c oxidase subunit I" + /protein_id="YP_003024028.1" + /db_xref="GeneID:4512" + /db_xref="HGNC:HGNC:7419" + /db_xref="MIM:516030" + /translation="MFADRWLFSTNHKDIGTLYLLFGAWAGVLGTALSLLIRAELGQP + GNLLGNDHIYNVIVTAHAFVMIFFMVMPIMIGGFGNWLVPLMIGAPDMAFPRMNNMSF + WLLPPSLLLLLASAMVEAGAGTGWTVYPPLAGNYSHPGASVDLTIFSLHLAGVSSILG + AINFITTIINMKPPAMTQYQTPLFVWSVLITAVLLLLSLPVLAAGITMLLTDRNLNTT + FFDPAGGGDPILYQHLFWFFGHPEVYILILPGFGMISHIVTYYSGKKEPFGYMGMVWA + MMSIGFLGFIVWAHHMFTVGMDVDTRAYFTSATMIIAIPTGVKVFSWLATLHGSNMKW + SAAVLWALGFIFLFTVGGLTGIVLANSSLDIVLHDTYYVVAHFHYVLSMGAVFAIMGG + FIHWFPLFSGYTLDQTYAKIHFTIMFIGVNLTFFPQHFLGLSGMPRRYSDYPDAYTTW + NILSSVGSFISLTAVMLMIFMIWEAFASKRKVLMVEEPSMNLEWLYGCPPPYHTFEEP + VYMKS" + gene complement(7446..7514) + /gene="TRNS1" + /gene_synonym="MTTS1" + /nomenclature="Official Symbol: MT-TS1 | Name: + mitochondrially encoded tRNA serine 1 (UCN)" + /db_xref="GeneID:4574" + /db_xref="HGNC:HGNC:7497" + /db_xref="MIM:590080" + tRNA complement(7446..7514) + /gene="TRNS1" + /gene_synonym="MTTS1" + /product="tRNA-Ser" + /note="NAR: 1697" + /anticodon=(pos:complement(7482..7484),aa:Ser,seq:tga) + /codon_recognized="UCN" + /db_xref="GeneID:4574" + /db_xref="HGNC:HGNC:7497" + /db_xref="MIM:590080" + gene 7518..7585 + /gene="TRND" + /gene_synonym="MTTD" + /nomenclature="Official Symbol: MT-TD | Name: + mitochondrially encoded tRNA aspartic acid | Provided by: + HGNC:HGNC:7478" + /db_xref="GeneID:4555" + /db_xref="HGNC:HGNC:7478" + /db_xref="MIM:590015" + tRNA 7518..7585 + /gene="TRND" + /gene_synonym="MTTD" + /product="tRNA-Asp" + /note="NAR: 0397" + /anticodon=(pos:7548..7550,aa:Asp,seq:gtc) + /codon_recognized="GAC" + /db_xref="GeneID:4555" + /db_xref="HGNC:HGNC:7478" + /db_xref="MIM:590015" + gene 7586..8269 + /gene="COX2" + /gene_synonym="COII; MTCO2" + /nomenclature="Official Symbol: MT-CO2 | Name: + mitochondrially encoded cytochrome c oxidase II | Provided + by: HGNC:HGNC:7421" + /db_xref="GeneID:4513" + /db_xref="HGNC:HGNC:7421" + /db_xref="MIM:516040" + CDS 7586..8269 + /gene="COX2" + /gene_synonym="COII; MTCO2" + /note="cytochrome c oxidase II" + /codon_start=1 + /transl_table=2 + /product="cytochrome c oxidase subunit II" + /protein_id="YP_003024029.1" + /db_xref="GeneID:4513" + /db_xref="HGNC:HGNC:7421" + /db_xref="MIM:516040" + /translation="MAHAAQVGLQDATSPIMEELITFHDHALMIIFLICFLVLYALFL + TLTTKLTNTNISDAQEMETVWTILPAIILVLIALPSLRILYMTDEVNDPSLTIKSIGH + QWYWTYEYTDYGGLIFNSYMLPPLFLEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVL + HSWAVPTLGLKTDAIPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKI + FEMGPVFTL" + gene 8295..8364 + /gene="TRNK" + /gene_synonym="MTTK" + /nomenclature="Official Symbol: MT-TK | Name: + mitochondrially encoded tRNA lysine | Provided by: + HGNC:HGNC:7489" + /db_xref="GeneID:4566" + /db_xref="HGNC:HGNC:7489" + /db_xref="MIM:590060" + tRNA 8295..8364 + /gene="TRNK" + /gene_synonym="MTTK" + /product="tRNA-Lys" + /note="NAR: 1197" + /anticodon=(pos:8323..8325,aa:Lys,seq:ttt) + /codon_recognized="AAA" + /db_xref="GeneID:4566" + /db_xref="HGNC:HGNC:7489" + /db_xref="MIM:590060" + gene 8366..8572 + /gene="ATP8" + /gene_synonym="ATPase8; MTATP8" + /nomenclature="Official Symbol: MT-ATP8 | Name: + mitochondrially encoded ATP synthase 8 | Provided by: + HGNC:HGNC:7415" + /db_xref="GeneID:4509" + /db_xref="HGNC:HGNC:7415" + /db_xref="MIM:516070" + CDS 8366..8572 + /gene="ATP8" + /gene_synonym="ATPase8; MTATP8" + /note="ATP synthase 8; ATPase subunit 8" + /codon_start=1 + /transl_table=2 + /product="ATP synthase F0 subunit 8" + /protein_id="YP_003024030.1" + /db_xref="GeneID:4509" + /db_xref="HGNC:HGNC:7415" + /db_xref="MIM:516070" + /translation="MPQLNTTVWPTMITPMLLTLFLITQLKMLNTNYHLPPSPKPMKM + KNYNKPWEPKWTKICSLHSLPPQS" + gene 8527..9207 + /gene="ATP6" + /gene_synonym="ATPase6; MTATP6" + /nomenclature="Official Symbol: MT-ATP6 | Name: + mitochondrially encoded ATP synthase 6 | Provided by: + HGNC:HGNC:7414" + /db_xref="GeneID:4508" + /db_xref="HGNC:HGNC:7414" + /db_xref="MIM:516060" + CDS 8527..9207 + /gene="ATP6" + /gene_synonym="ATPase6; MTATP6" + /note="ATP synthase 6; ATPase subunit 6" + /codon_start=1 + /transl_table=2 + /product="ATP synthase F0 subunit 6" + /protein_id="YP_003024031.1" + /db_xref="GeneID:4508" + /db_xref="HGNC:HGNC:7414" + /db_xref="MIM:516060" + /translation="MNENLFASFIAPTILGLPAAVLIILFPPLLIPTSKYLINNRLIT + TQQWLIKLTSKQMMTMHNTKGRTWSLMLVSLIIFIATTNLLGLLPHSFTPTTQLSMNL + AMAIPLWAGTVIMGFRSKIKNALAHFLPQGTPTPLIPMLVIIETISLLIQPMALAVRL + TANITAGHLLMHLIGSATLAMSTINLPSTLIIFTILILLTILEIAVALIQAYVFTLLV + SLYLHDNT" + gene 9207..9990 + /gene="COX3" + /gene_synonym="COIII; MTCO3" + /nomenclature="Official Symbol: MT-CO3 | Name: + mitochondrially encoded cytochrome c oxidase III | + Provided by: HGNC:HGNC:7422" + /db_xref="GeneID:4514" + /db_xref="HGNC:HGNC:7422" + /db_xref="MIM:516050" + CDS 9207..9990 + /gene="COX3" + /gene_synonym="COIII; MTCO3" + /note="cytochrome c oxidase III; TAA stop codon is + completed by the addition of 3' A residues to the mRNA" + /codon_start=1 + /transl_except=(pos:9990,aa:TERM) + /transl_table=2 + /product="cytochrome c oxidase subunit III" + /protein_id="YP_003024032.1" + /db_xref="GeneID:4514" + /db_xref="HGNC:HGNC:7422" + /db_xref="MIM:516050" + /translation="MTHQSHAYHMVKPSPWPLTGALSALLMTSGLAMWFHFHSMTLLM + LGLLTNTLTMYQWWRDVTRESTYQGHHTPPVQKGLRYGMILFITSEVFFFAGFFWAFY + HSSLAPTPQLGGHWPPTGITPLNPLEVPLLNTSVLLASGVSITWAHHSLMENNRNQMI + QALLITILLGLYFTLLQASEYFESPFTISDGIYGSTFFVATGFHGLHVIIGSTFLTIC + FIRQLMFHFTSKHHFGFEAAAWYWHFVDVVWLFLYVSIYWWGS" + gene 9991..10058 + /gene="TRNG" + /gene_synonym="MTTG" + /nomenclature="Official Symbol: MT-TG | Name: + mitochondrially encoded tRNA glycine | Provided by: + HGNC:HGNC:7486" + /db_xref="GeneID:4563" + /db_xref="HGNC:HGNC:7486" + /db_xref="MIM:590035" + tRNA 9991..10058 + /gene="TRNG" + /gene_synonym="MTTG" + /product="tRNA-Gly" + /note="NAR: 0797" + /anticodon=(pos:10021..10023,aa:Gly,seq:tcc) + /codon_recognized="GGA" + /db_xref="GeneID:4563" + /db_xref="HGNC:HGNC:7486" + /db_xref="MIM:590035" + gene 10059..10404 + /gene="ND3" + /gene_synonym="MTND3" + /nomenclature="Official Symbol: MT-ND3 | Name: + mitochondrially encoded NADH dehydrogenase 3 | Provided + by: HGNC:HGNC:7458" + /db_xref="GeneID:4537" + /db_xref="HGNC:HGNC:7458" + /db_xref="MIM:516002" + CDS 10059..10404 + /gene="ND3" + /gene_synonym="MTND3" + /note="NADH dehydrogenase, subunit 3 (complex I); TAA stop + codon is completed by the addition of 3' A residues to the + mRNA" + /codon_start=1 + /transl_except=(pos:10404,aa:TERM) + /transl_table=2 + /product="NADH dehydrogenase subunit 3" + /protein_id="YP_003024033.1" + /db_xref="GeneID:4537" + /db_xref="HGNC:HGNC:7458" + /db_xref="MIM:516002" + /translation="MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPM + SPARVPFSMKFFLVAITFLLFDLEIALLLPLPWALQTTNLPLMVMSSLLLIIILALSL + AYEWLQKGLDWTE" + gene 10405..10469 + /gene="TRNR" + /gene_synonym="MTTR" + /nomenclature="Official Symbol: MT-TR | Name: + mitochondrially encoded tRNA arginine | Provided by: + HGNC:HGNC:7496" + /db_xref="GeneID:4573" + /db_xref="HGNC:HGNC:7496" + /db_xref="MIM:590005" + tRNA 10405..10469 + /gene="TRNR" + /gene_synonym="MTTR" + /product="tRNA-Arg" + /note="NAR: 0197" + /anticodon=(pos:10435..10437,aa:Arg,seq:tcg) + /codon_recognized="CGA" + /db_xref="GeneID:4573" + /db_xref="HGNC:HGNC:7496" + /db_xref="MIM:590005" + gene 10470..10766 + /gene="ND4L" + /gene_synonym="MTND4L" + /nomenclature="Official Symbol: MT-ND4L | Name: + mitochondrially encoded NADH 4L dehydrogenase | Provided + by: HGNC:HGNC:7460" + /db_xref="GeneID:4539" + /db_xref="HGNC:HGNC:7460" + /db_xref="MIM:516004" + CDS 10470..10766 + /gene="ND4L" + /gene_synonym="MTND4L" + /note="NADH dehydrogenase, subunit 4L (complex I)" + /codon_start=1 + /transl_table=2 + /product="NADH dehydrogenase subunit 4L" + /protein_id="YP_003024034.1" + /db_xref="GeneID:4539" + /db_xref="HGNC:HGNC:7460" + /db_xref="MIM:516004" + /translation="MPLIYMNIMLAFTISLLGMLVYRSHLMSSLLCLEGMMLSLFIMA + TLMTLNTHSLLANIVPIAMLVFAACEAAVGLALLVSISNTYGLDYVHNLNLLQC" + gene 10760..12137 + /gene="ND4" + /gene_synonym="MTND4" + /nomenclature="Official Symbol: MT-ND4 | Name: + mitochondrially encoded NADH dehydrogenase 4 | Provided + by: HGNC:HGNC:7459" + /db_xref="GeneID:4538" + /db_xref="HGNC:HGNC:7459" + /db_xref="MIM:516003" + CDS 10760..12137 + /gene="ND4" + /gene_synonym="MTND4" + /note="NADH dehydrogenase, subunit 4 (complex I); TAA stop + codon is completed by the addition of 3' A residues to the + mRNA" + /codon_start=1 + /transl_except=(pos:12137,aa:TERM) + /transl_table=2 + /product="NADH dehydrogenase subunit 4" + /protein_id="YP_003024035.1" + /db_xref="GeneID:4538" + /db_xref="HGNC:HGNC:7459" + /db_xref="MIM:516003" + /translation="MLKLIVPTIMLLPLTWLSKKHMIWINTTTHSLIISIIPLLFFNQ + INNNLFSCSPTFSSDPLTTPLLMLTTWLLPLTIMASQRHLSSEPLSRKKLYLSMLISL + QISLIMTFTATELIMFYIFFETTLIPTLAIITRWGNQPERLNAGTYFLFYTLVGSLPL + LIALIYTHNTLGSLNILLLTLTAQELSNSWANNLMWLAYTMAFMVKMPLYGLHLWLPK + AHVEAPIAGSMVLAAVLLKLGGYGMMRLTLILNPLTKHMAYPFLVLSLWGMIMTSSIC + LRQTDLKSLIAYSSISHMALVVTAILIQTPWSFTGAVILMIAHGLTSSLLFCLANSNY + ERTHSRIMILSQGLQTLLPLMAFWWLLASLANLALPPTINLLGELSVLVTTFSWSNIT + LLLTGLNMLVTALYSLYMFTTTQWGSLTHHINNMKPSFTRENTLMFMHLSPILLLSLN + PDIITGFSS" + gene 12138..12206 + /gene="TRNH" + /gene_synonym="MTTH" + /nomenclature="Official Symbol: MT-TH | Name: + mitochondrially encoded tRNA histidine | Provided by: + HGNC:HGNC:7487" + /db_xref="GeneID:4564" + /db_xref="HGNC:HGNC:7487" + /db_xref="MIM:590040" + tRNA 12138..12206 + /gene="TRNH" + /gene_synonym="MTTH" + /product="tRNA-His" + /note="NAR: 0897" + /anticodon=(pos:12168..12170,aa:His,seq:gtg) + /codon_recognized="CAC" + /db_xref="GeneID:4564" + /db_xref="HGNC:HGNC:7487" + /db_xref="MIM:590040" + gene 12207..12265 + /gene="TRNS2" + /gene_synonym="MTTS2" + /nomenclature="Official Symbol: MT-TS2 | Name: + mitochondrially encoded tRNA serine 2 (AGU/C) | Provided + by: HGNC:HGNC:7498" + /db_xref="GeneID:4575" + /db_xref="HGNC:HGNC:7498" + /db_xref="MIM:590085" + tRNA 12207..12265 + /gene="TRNS2" + /gene_synonym="MTTS2" + /product="tRNA-Ser" + /note="NAR: 1656" + /anticodon=(pos:12226..12228,aa:Ser,seq:gct) + /codon_recognized="AGY" + /db_xref="GeneID:4575" + /db_xref="HGNC:HGNC:7498" + /db_xref="MIM:590085" + gene 12266..12336 + /gene="TRNL2" + /gene_synonym="MTTL2" + /nomenclature="Official Symbol: MT-TL2 | Name: + mitochondrially encoded tRNA leucine 2 (CUN) | Provided + by: HGNC:HGNC:7491" + /db_xref="GeneID:4568" + /db_xref="HGNC:HGNC:7491" + /db_xref="MIM:590055" + tRNA 12266..12336 + /gene="TRNL2" + /gene_synonym="MTTL2" + /product="tRNA-Leu" + /note="NAR: 1097" + /anticodon=(pos:12298..12300,aa:Leu,seq:tag) + /codon_recognized="CUN" + /db_xref="GeneID:4568" + /db_xref="HGNC:HGNC:7491" + /db_xref="MIM:590055" + gene 12337..14148 + /gene="ND5" + /gene_synonym="MTND5" + /nomenclature="Official Symbol: MT-ND5 | Name: + mitochondrially encoded NADH dehydrogenase 5 | Provided + by: HGNC:HGNC:7461" + /db_xref="GeneID:4540" + /db_xref="HGNC:HGNC:7461" + /db_xref="MIM:516005" + CDS 12337..14148 + /gene="ND5" + /gene_synonym="MTND5" + /note="NADH dehydrogenase, subunit 5 (complex I)" + /codon_start=1 + /transl_table=2 + /product="NADH dehydrogenase subunit 5" + /protein_id="YP_003024036.1" + /db_xref="GeneID:4540" + /db_xref="HGNC:HGNC:7461" + /db_xref="MIM:516005" + /translation="MTMHTTMTTLTLTSLIPPILTTLVNPNKKNSYPHYVKSIVASTF + IISLFPTTMFMCLDQEVIISNWHWATTQTTQLSLSFKLDYFSMMFIPVALFVTWSIME + FSLWYMNSDPNINQFFKYLLIFLITMLILVTANNLFQLFIGWEGVGIMSFLLISWWYA + RADANTAAIQAILYNRIGDIGFILALAWFILHSNSWDPQQMALLNANPSLTPLLGLLL + AAAGKSAQLGLHPWLPSAMEGPTPVSALLHSSTMVVAGIFLLIRFHPLAENSPLIQTL + TLCLGAITTLFAAVCALTQNDIKKIVAFSTSSQLGLMMVTIGINQPHLAFLHICTHAF + FKAMLFMCSGSIIHNLNNEQDIRKMGGLLKTMPLTSTSLTIGSLALAGMPFLTGFYSK + DHIIETANMSYTNAWALSITLIATSLTSAYSTRMILLTLTGQPRFPTLTNINENNPTL + LNPIKRLAAGSLFAGFLITNNISPASPFQTTIPLYLKLTALAVTFLGLLTALDLNYLT + NKLKMKSPLCTFYFSNMLGFYPSITHRTIPYLGLLTSQNLPLLLLDLTWLEKLLPKTI + SQHQISTSIITSTQKGMIKLYFLSFFFPLILTLLLIT" + gene complement(14149..14673) + /gene="ND6" + /gene_synonym="MTND6" + /nomenclature="Official Symbol: MT-ND6 | Name: + mitochondrially encoded NADH dehydrogenase 6 | Provided + by: HGNC:HGNC:7462" + /db_xref="GeneID:4541" + /db_xref="HGNC:HGNC:7462" + /db_xref="MIM:516006" + CDS complement(14149..14673) + /gene="ND6" + /gene_synonym="MTND6" + /note="NADH dehydrogenase, subunit 6 (complex I)" + /codon_start=1 + /transl_table=2 + /product="NADH dehydrogenase subunit 6" + /protein_id="YP_003024037.1" + /db_xref="GeneID:4541" + /db_xref="HGNC:HGNC:7462" + /db_xref="MIM:516006" + /translation="MMYALFLLSVGLVMGFVGFSSKPSPIYGGLVLIVSGVVGCVIIL + NFGGGYMGLMVFLIYLGGMMVVFGYTTAMAIEEYPEAWGSGVEVLVSVLVGLAMEVGL + VLWVKEYDGVVVVVNFNSVGSWMIYEGEGSGLIREDPIGAGALYDYGRWLVVVTGWTL + FVGVYIVIEIARGN" + gene complement(14674..14742) + /gene="TRNE" + /gene_synonym="MTTE" + /nomenclature="Official Symbol: MT-TE | Name: + mitochondrially encoded tRNA glutamic acid | Provided by: + HGNC:HGNC:7479" + /db_xref="GeneID:4556" + /db_xref="HGNC:HGNC:7479" + /db_xref="MIM:590025" + tRNA complement(14674..14742) + /gene="TRNE" + /gene_synonym="MTTE" + /product="tRNA-Glu" + /note="NAR: 0697" + /anticodon=(pos:complement(14710..14712),aa:Glu,seq:ttc) + /codon_recognized="GAA" + /db_xref="GeneID:4556" + /db_xref="HGNC:HGNC:7479" + /db_xref="MIM:590025" + gene 14747..15887 + /gene="CYTB" + /gene_synonym="MTCYB" + /nomenclature="Official Symbol: MT-CYB | Name: + mitochondrially encoded cytochrome b | Provided by: + HGNC:HGNC:7427" + /db_xref="GeneID:4519" + /db_xref="HGNC:HGNC:7427" + /db_xref="MIM:516020" + CDS 14747..15887 + /gene="CYTB" + /gene_synonym="MTCYB" + /note="TAA stop codon is completed by the addition of 3' A + residues to the mRNA" + /codon_start=1 + /transl_except=(pos:15887,aa:TERM) + /transl_table=2 + /product="cytochrome b" + /protein_id="YP_003024038.1" + /db_xref="GeneID:4519" + /db_xref="HGNC:HGNC:7427" + /db_xref="MIM:516020" + /translation="MTPMRKTNPLMKLINHSFIDLPTPSNISAWWNFGSLLGACLILQ + ITTGLFLAMHYSPDASTAFSSIAHITRDVNYGWIIRYLHANGASMFFICLFLHIGRGL + YYGSFLYSETWNIGIILLLATMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDL + VQWIWGGYSVDSPTLTRFFTFHFILPFIIAALATLHLLFLHETGSNNPLGITSHSDKI + TFHPYYTIKDALGLLLFLLSLMTLTLFSPDLLGDPDNYTLANPLNTPPHIKPEWYFLF + AYTILRSVPNKLGGVLALLLSILILAMIPILHMSKQQSMMFRPLSQSLYWLLAADLLI + LTWIGGQPVSYPFTIIGQVASVLYFTTILILMPTISLIENKMLKWA" + gene 15888..15953 + /gene="TRNT" + /gene_synonym="MTTT" + /nomenclature="Official Symbol: MT-TT | Name: + mitochondrially encoded tRNA threonine | Provided by: + HGNC:HGNC:7499" + /db_xref="GeneID:4576" + /db_xref="HGNC:HGNC:7499" + /db_xref="MIM:590090" + tRNA 15888..15953 + /gene="TRNT" + /gene_synonym="MTTT" + /product="tRNA-Thr" + /note="NAR: 1797" + /anticodon=(pos:15919..15921,aa:Thr,seq:tgt) + /codon_recognized="ACA" + /db_xref="GeneID:4576" + /db_xref="HGNC:HGNC:7499" + /db_xref="MIM:590090" + gene complement(15956..16023) + /gene="TRNP" + /gene_synonym="MTTP" + /nomenclature="Official Symbol: MT-TP | Name: + mitochondrially encoded tRNA proline | Provided by: + HGNC:HGNC:7494" + /db_xref="GeneID:4571" + /db_xref="HGNC:HGNC:7494" + /db_xref="MIM:590075" + tRNA complement(15956..16023) + /gene="TRNP" + /gene_synonym="MTTP" + /product="tRNA-Pro" + /note="NAR: 1597" + /anticodon=(pos:complement(15990..15992),aa:Pro,seq:tgg) + /codon_recognized="CCA" + /db_xref="GeneID:4571" + /db_xref="HGNC:HGNC:7494" + /db_xref="MIM:590075" +ORIGIN + 1 gatcacaggt ctatcaccct attaaccact cacgggagct ctccatgcat ttggtatttt + 61 cgtctggggg gtatgcacgc gatagcattg cgagacgctg gagccggagc accctatgtc + 121 gcagtatctg tctttgattc ctgcctcatc ctattattta tcgcacctac gttcaatatt + 181 acaggcgaac atacttacta aagtgtgtta attaattaat gcttgtagga cataataata + 241 acaattgaat gtctgcacag ccactttcca cacagacatc ataacaaaaa atttccacca + 301 aaccccccct cccccgcttc tggccacagc acttaaacac atctctgcca aaccccaaaa + 361 acaaagaacc ctaacaccag cctaaccaga tttcaaattt tatcttttgg cggtatgcac + 421 ttttaacagt caccccccaa ctaacacatt attttcccct cccactccca tactactaat + 481 ctcatcaata caacccccgc ccatcctacc cagcacacac acaccgctgc taaccccata + 541 ccccgaacca accaaacccc aaagacaccc cccacagttt atgtagctta cctcctcaaa + 601 gcaatacact gaaaatgttt agacgggctc acatcacccc ataaacaaat aggtttggtc + 661 ctagcctttc tattagctct tagtaagatt acacatgcaa gcatccccgt tccagtgagt + 721 tcaccctcta aatcaccacg atcaaaagga acaagcatca agcacgcagc aatgcagctc + 781 aaaacgctta gcctagccac acccccacgg gaaacagcag tgattaacct ttagcaataa + 841 acgaaagttt aactaagcta tactaacccc agggttggtc aatttcgtgc cagccaccgc + 901 ggtcacacga ttaacccaag tcaatagaag ccggcgtaaa gagtgtttta gatcaccccc + 961 tccccaataa agctaaaact cacctgagtt gtaaaaaact ccagttgaca caaaatagac + 1021 tacgaaagtg gctttaacat atctgaacac acaatagcta agacccaaac tgggattaga + 1081 taccccacta tgcttagccc taaacctcaa cagttaaatc aacaaaactg ctcgccagaa + 1141 cactacgagc cacagcttaa aactcaaagg acctggcggt gcttcatatc cctctagagg + 1201 agcctgttct gtaatcgata aaccccgatc aacctcacca cctcttgctc agcctatata + 1261 ccgccatctt cagcaaaccc tgatgaaggc tacaaagtaa gcgcaagtac ccacgtaaag + 1321 acgttaggtc aaggtgtagc ccatgaggtg gcaagaaatg ggctacattt tctaccccag + 1381 aaaactacga tagcccttat gaaacttaag ggtcgaaggt ggatttagca gtaaactaag + 1441 agtagagtgc ttagttgaac agggccctga agcgcgtaca caccgcccgt caccctcctc + 1501 aagtatactt caaaggacat ttaactaaaa cccctacgca tttatataga ggagacaagt + 1561 cgtaacatgg taagtgtact ggaaagtgca cttggacgaa ccagagtgta gcttaacaca + 1621 aagcacccaa cttacactta ggagatttca acttaacttg accgctctga gctaaaccta + 1681 gccccaaacc cactccacct tactaccaga caaccttagc caaaccattt acccaaataa + 1741 agtataggcg atagaaattg aaacctggcg caatagatat agtaccgcaa gggaaagatg + 1801 aaaaattata accaagcata atatagcaag gactaacccc tataccttct gcataatgaa + 1861 ttaactagaa ataactttgc aaggagagcc aaagctaaga cccccgaaac cagacgagct + 1921 acctaagaac agctaaaaga gcacacccgt ctatgtagca aaatagtggg aagatttata + 1981 ggtagaggcg acaaacctac cgagcctggt gatagctggt tgtccaagat agaatcttag + 2041 ttcaacttta aatttgccca cagaaccctc taaatcccct tgtaaattta actgttagtc + 2101 caaagaggaa cagctctttg gacactagga aaaaaccttg tagagagagt aaaaaattta + 2161 acacccatag taggcctaaa agcagccacc aattaagaaa gcgttcaagc tcaacaccca + 2221 ctacctaaaa aatcccaaac atataactga actcctcaca cccaattgga ccaatctatc + 2281 accctataga agaactaatg ttagtataag taacatgaaa acattctcct ccgcataagc + 2341 ctgcgtcaga ttaaaacact gaactgacaa ttaacagccc aatatctaca atcaaccaac + 2401 aagtcattat taccctcact gtcaacccaa cacaggcatg ctcataagga aaggttaaaa + 2461 aaagtaaaag gaactcggca aatcttaccc cgcctgttta ccaaaaacat cacctctagc + 2521 atcaccagta ttagaggcac cgcctgccca gtgacacatg tttaacggcc gcggtaccct + 2581 aaccgtgcaa aggtagcata atcacttgtt ccttaaatag ggacctgtat gaatggctcc + 2641 acgagggttc agctgtctct tacttttaac cagtgaaatt gacctgcccg tgaagaggcg + 2701 ggcataacac agcaagacga gaagacccta tggagcttta atttattaat gcaaacagta + 2761 cctaacaaac ccacaggtcc taaactacca aacctgcatt aaaaatttcg gttggggcga + 2821 cctcggagca gaacccaacc tccgagcagt acatgctaag acttcaccag tcaaagcgaa + 2881 ctactatact caattgatcc aataacttga ccaacggaac aagttaccct agggataaca + 2941 gcgcaatcct attctagagt ccatatcaac aatagggttt acgacctcga tgttggatca + 3001 ggacatcccg atggtgcagc cgctattaaa ggttcgtttg ttcaacgatt aaagtcctac + 3061 gtgatctgag ttcagaccgg agtaatccag gtcggtttct atctacnttc aaattcctcc + 3121 ctgtacgaaa ggacaagaga aataaggcct acttcacaaa gcgccttccc ccgtaaatga + 3181 tatcatctca acttagtatt atacccacac ccacccaaga acagggtttg ttaagatggc + 3241 agagcccggt aatcgcataa aacttaaaac tttacagtca gaggttcaat tcctcttctt + 3301 aacaacatac ccatggccaa cctcctactc ctcattgtac ccattctaat cgcaatggca + 3361 ttcctaatgc ttaccgaacg aaaaattcta ggctatatac aactacgcaa aggccccaac + 3421 gttgtaggcc cctacgggct actacaaccc ttcgctgacg ccataaaact cttcaccaaa + 3481 gagcccctaa aacccgccac atctaccatc accctctaca tcaccgcccc gaccttagct + 3541 ctcaccatcg ctcttctact atgaaccccc ctccccatac ccaaccccct ggtcaacctc + 3601 aacctaggcc tcctatttat tctagccacc tctagcctag ccgtttactc aatcctctga + 3661 tcagggtgag catcaaactc aaactacgcc ctgatcggcg cactgcgagc agtagcccaa + 3721 acaatctcat atgaagtcac cctagccatc attctactat caacattact aataagtggc + 3781 tcctttaacc tctccaccct tatcacaaca caagaacacc tctgattact cctgccatca + 3841 tgacccttgg ccataatatg atttatctcc acactagcag agaccaaccg aacccccttc + 3901 gaccttgccg aaggggagtc cgaactagtc tcaggcttca acatcgaata cgccgcaggc + 3961 cccttcgccc tattcttcat agccgaatac acaaacatta ttataataaa caccctcacc + 4021 actacaatct tcctaggaac aacatatgac gcactctccc ctgaactcta cacaacatat + 4081 tttgtcacca agaccctact tctaacctcc ctgttcttat gaattcgaac agcatacccc + 4141 cgattccgct acgaccaact catacacctc ctatgaaaaa acttcctacc actcacccta + 4201 gcattactta tatgatatgt ctccataccc attacaatct ccagcattcc ccctcaaacc + 4261 taagaaatat gtctgataaa agagttactt tgatagagta aataatagga gcttaaaccc + 4321 ccttatttct aggactatga gaatcgaacc catccctgag aatccaaaat tctccgtgcc + 4381 acctatcaca ccccatccta aagtaaggtc agctaaataa gctatcgggc ccataccccg + 4441 aaaatgttgg ttataccctt cccgtactaa ttaatcccct ggcccaaccc gtcatctact + 4501 ctaccatctt tgcaggcaca ctcatcacag cgctaagctc gcactgattt tttacctgag + 4561 taggcctaga aataaacatg ctagctttta ttccagttct aaccaaaaaa ataaaccctc + 4621 gttccacaga agctgccatc aagtatttcc tcacgcaagc aaccgcatcc ataatccttc + 4681 taatagctat cctcttcaac aatatactct ccggacaatg aaccataacc aatactacca + 4741 atcaatactc atcattaata atcataatag ctatagcaat aaaactagga atagccccct + 4801 ttcacttctg agtcccagag gttacccaag gcacccctct gacatccggc ctgcttcttc + 4861 tcacatgaca aaaactagcc cccatctcaa tcatatacca aatctctccc tcactaaacg + 4921 taagccttct cctcactctc tcaatcttat ccatcatagc aggcagttga ggtggattaa + 4981 accaaaccca gctacgcaaa atcttagcat actcctcaat tacccacata ggatgaataa + 5041 tagcagttct accgtacaac cctaacataa ccattcttaa tttaactatt tatattatcc + 5101 taactactac cgcattccta ctactcaact taaactccag caccacgacc ctactactat + 5161 ctcgcacctg aaacaagcta acatgactaa cacccttaat tccatccacc ctcctctccc + 5221 taggaggcct gcccccgcta accggctttt tgcccaaatg ggccattatc gaagaattca + 5281 caaaaaacaa tagcctcatc atccccacca tcatagccac catcaccctc cttaacctct + 5341 acttctacct acgcctaatc tactccacct caatcacact actccccata tctaacaacg + 5401 taaaaataaa atgacagttt gaacatacaa aacccacccc attcctcccc acactcatcg + 5461 cccttaccac gctactccta cctatctccc cttttatact aataatctta tagaaattta + 5521 ggttaaatac agaccaagag ccttcaaagc cctcagtaag ttgcaatact taatttctgt + 5581 aacagctaag gactgcaaaa ccccactctg catcaactga acgcaaatca gccactttaa + 5641 ttaagctaag cccttactag accaatggga cttaaaccca caaacactta gttaacagct + 5701 aagcacccta atcaactggc ttcaatctac ttctcccgcc gccgggaaaa aaggcgggag + 5761 aagccccggc aggtttgaag ctgcttcttc gaatttgcaa ttcaatatga aaatcacctc + 5821 ggagctggta aaaagaggcc taacccctgt ctttagattt acagtccaat gcttcactca + 5881 gccattttac ctcaccccca ctgatgttcg ccgaccgttg actattctct acaaaccaca + 5941 aagacattgg aacactatac ctattattcg gcgcatgagc tggagtccta ggcacagctc + 6001 taagcctcct tattcgagcc gagctgggcc agccaggcaa ccttctaggt aacgaccaca + 6061 tctacaacgt tatcgtcaca gcccatgcat ttgtaataat cttcttcata gtaataccca + 6121 tcataatcgg aggctttggc aactgactag ttcccctaat aatcggtgcc cccgatatgg + 6181 cgtttccccg cataaacaac ataagcttct gactcttacc tccctctctc ctactcctgc + 6241 tcgcatctgc tatagtggag gccggagcag gaacaggttg aacagtctac cctcccttag + 6301 cagggaacta ctcccaccct ggagcctccg tagacctaac catcttctcc ttacacctag + 6361 caggtgtctc ctctatctta ggggccatca atttcatcac aacaattatc aatataaaac + 6421 cccctgccat aacccaatac caaacgcccc tcttcgtctg atccgtccta atcacagcag + 6481 tcctacttct cctatctctc ccagtcctag ctgctggcat cactatacta ctaacagacc + 6541 gcaacctcaa caccaccttc ttcgaccccg ccggaggagg agaccccatt ctataccaac + 6601 acctattctg atttttcggt caccctgaag tttatattct tatcctacca ggcttcggaa + 6661 taatctccca tattgtaact tactactccg gaaaaaaaga accatttgga tacataggta + 6721 tggtctgagc tatgatatca attggcttcc tagggtttat cgtgtgagca caccatatat + 6781 ttacagtagg aatagacgta gacacacgag catatttcac ctccgctacc ataatcatcg + 6841 ctatccccac cggcgtcaaa gtatttagct gactcgccac actccacgga agcaatatga + 6901 aatgatctgc tgcagtgctc tgagccctag gattcatctt tcttttcacc gtaggtggcc + 6961 tgactggcat tgtattagca aactcatcac tagacatcgt actacacgac acgtactacg + 7021 ttgtagccca cttccactat gtcctatcaa taggagctgt atttgccatc ataggaggct + 7081 tcattcactg atttccccta ttctcaggct acaccctaga ccaaacctac gccaaaatcc + 7141 atttcactat catattcatc ggcgtaaatc taactttctt cccacaacac tttctcggcc + 7201 tatccggaat gccccgacgt tactcggact accccgatgc atacaccaca tgaaacatcc + 7261 tatcatctgt aggctcattc atttctctaa cagcagtaat attaataatt ttcatgattt + 7321 gagaagcctt cgcttcgaag cgaaaagtcc taatagtaga agaaccctcc ataaacctgg + 7381 agtgactata tggatgcccc ccaccctacc acacattcga agaacccgta tacataaaat + 7441 ctagacaaaa aaggaaggaa tcgaaccccc caaagctggt ttcaagccaa ccccatggcc + 7501 tccatgactt tttcaaaaag gtattagaaa aaccatttca taactttgtc aaagttaaat + 7561 tataggctaa atcctatata tcttaatggc acatgcagcg caagtaggtc tacaagacgc + 7621 tacttcccct atcatagaag agcttatcac ctttcatgat cacgccctca taatcatttt + 7681 ccttatctgc ttcctagtcc tgtatgccct tttcctaaca ctcacaacaa aactaactaa + 7741 tactaacatc tcagacgctc aggaaataga aaccgtctga actatcctgc ccgccatcat + 7801 cctagtcctc atcgccctcc catccctacg catcctttac ataacagacg aggtcaacga + 7861 tccctccctt accatcaaat caattggcca ccaatggtac tgaacctacg agtacaccga + 7921 ctacggcgga ctaatcttca actcctacat acttccccca ttattcctag aaccaggcga + 7981 cctgcgactc cttgacgttg acaatcgagt agtactcccg attgaagccc ccattcgtat + 8041 aataattaca tcacaagacg tcttgcactc atgagctgtc cccacattag gcttaaaaac + 8101 agatgcaatt cccggacgtc taaaccaaac cactttcacc gctacacgac cgggggtata + 8161 ctacggtcaa tgctctgaaa tctgtggagc aaaccacagt ttcatgccca tcgtcctaga + 8221 attaattccc ctaaaaatct ttgaaatagg gcccgtattt accctatagc accccctcta + 8281 ccccctctag agcccactgt aaagctaact tagcattaac cttttaagtt aaagattaag + 8341 agaaccaaca cctctttaca gtgaaatgcc ccaactaaat actaccgtat ggcccaccat + 8401 aattaccccc atactcctta cactattcct catcacccaa ctaaaaatat taaacacaaa + 8461 ctaccaccta cctccctcac caaagcccat aaaaataaaa aattataaca aaccctgaga + 8521 accaaaatga acgaaaatct gttcgcttca ttcattgccc ccacaatcct aggcctaccc + 8581 gccgcagtac tgatcattct atttccccct ctattgatcc ccacctccaa atatctcatc + 8641 aacaaccgac taatcaccac ccaacaatga ctaatcaaac taacctcaaa acaaatgata + 8701 accatacaca acactaaagg acgaacctga tctcttatac tagtatcctt aatcattttt + 8761 attgccacaa ctaacctcct cggactcctg cctcactcat ttacaccaac cacccaacta + 8821 tctataaacc tagccatggc catcccctta tgagcgggca cagtgattat aggctttcgc + 8881 tctaagatta aaaatgccct agcccacttc ttaccacaag gcacacctac accccttatc + 8941 cccatactag ttattatcga aaccatcagc ctactcattc aaccaatagc cctggccgta + 9001 cgcctaaccg ctaacattac tgcaggccac ctactcatgc acctaattgg aagcgccacc + 9061 ctagcaatat caaccattaa ccttccctct acacttatca tcttcacaat tctaattcta + 9121 ctgactatcc tagaaatcgc tgtcgcctta atccaagcct acgttttcac acttctagta + 9181 agcctctacc tgcacgacaa cacataatga cccaccaatc acatgcctat catatagtaa + 9241 aacccagccc atgaccccta acaggggccc tctcagccct cctaatgacc tccggcctag + 9301 ccatgtgatt tcacttccac tccataacgc tcctcatact aggcctacta accaacacac + 9361 taaccatata ccaatgatgg cgcgatgtaa cacgagaaag cacataccaa ggccaccaca + 9421 caccacctgt ccaaaaaggc cttcgatacg ggataatcct atttattacc tcagaagttt + 9481 ttttcttcgc aggatttttc tgagcctttt accactccag cctagcccct accccccaat + 9541 taggagggca ctggccccca acaggcatca ccccgctaaa tcccctagaa gtcccactcc + 9601 taaacacatc cgtattactc gcatcaggag tatcaatcac ctgagctcac catagtctaa + 9661 tagaaaacaa ccgaaaccaa ataattcaag cactgcttat tacaatttta ctgggtctct + 9721 attttaccct cctacaagcc tcagagtact tcgagtctcc cttcaccatt tccgacggca + 9781 tctacggctc aacatttttt gtagccacag gcttccacgg acttcacgtc attattggct + 9841 caactttcct cactatctgc ttcatccgcc aactaatatt tcactttaca tccaaacatc + 9901 actttggctt cgaagccgcc gcctgatact ggcattttgt agatgtggtt tgactatttc + 9961 tgtatgtctc catctattga tgagggtctt actcttttag tataaatagt accgttaact + 10021 tccaattaac tagttttgac aacattcaaa aaagagtaat aaacttcgcc ttaattttaa + 10081 taatcaacac cctcctagcc ttactactaa taattattac attttgacta ccacaactca + 10141 acggctacat agaaaaatcc accccttacg agtgcggctt cgaccctata tcccccgccc + 10201 gcgtcccttt ctccataaaa ttcttcttag tagctattac cttcttatta tttgatctag + 10261 aaattgccct ccttttaccc ctaccatgag ccctacaaac aactaacctg ccactaatag + 10321 ttatgtcatc cctcttatta atcatcatcc tagccctaag tctggcctat gagtgactac + 10381 aaaaaggatt agactgaacc gaattggtat atagtttaaa caaaacgaat gatttcgact + 10441 cattaaatta tgataatcat atttaccaaa tgcccctcat ttacataaat attatactag + 10501 catttaccat ctcacttcta ggaatactag tatatcgctc acacctcata tcctccctac + 10561 tatgcctaga aggaataata ctatcgctgt tcattatagc tactctcata accctcaaca + 10621 cccactccct cttagccaat attgtgccta ttgccatact agtctttgcc gcctgcgaag + 10681 cagcggtggg cctagcccta ctagtctcaa tctccaacac atatggccta gactacgtac + 10741 ataacctaaa cctactccaa tgctaaaact aatcgtccca acaattatat tactaccact + 10801 gacatgactt tccaaaaaac acataatttg aatcaacaca accacccaca gcctaattat + 10861 tagcatcatc cctctactat tttttaacca aatcaacaac aacctattta gctgttcccc + 10921 aaccttttcc tccgaccccc taacaacccc cctcctaata ctaactacct gactcctacc + 10981 cctcacaatc atggcaagcc aacgccactt atccagtgaa ccactatcac gaaaaaaact + 11041 ctacctctct atactaatct ccctacaaat ctccttaatt ataacattca cagccacaga + 11101 actaatcata ttttatatct tcttcgaaac cacacttatc cccaccttgg ctatcatcac + 11161 ccgatgaggc aaccagccag aacgcctgaa cgcaggcaca tacttcctat tctacaccct + 11221 agtaggctcc cttcccctac tcatcgcact aatttacact cacaacaccc taggctcact + 11281 aaacattcta ctactcactc tcactgccca agaactatca aactcctgag ccaacaactt + 11341 aatatgacta gcttacacaa tagcttttat agtaaagata cctctttacg gactccactt + 11401 atgactccct aaagcccatg tcgaagcccc catcgctggg tcaatagtac ttgccgcagt + 11461 actcttaaaa ctaggcggct atggtataat acgcctcaca ctcattctca accccctgac + 11521 aaaacacata gcctacccct tccttgtact atccctatga ggcataatta taacaagctc + 11581 catctgccta cgacaaacag acctaaaatc gctcattgca tactcttcaa tcagccacat + 11641 agccctcgta gtaacagcca ttctcatcca aaccccctga agcttcaccg gcgcagtcat + 11701 tctcataatc gcccacgggc ttacatcctc attactattc tgcctagcaa actcaaacta + 11761 cgaacgcact cacagtcgca tcataatcct ctctcaagga cttcaaactc tactcccact + 11821 aatagctttt tgatgacttc tagcaagcct cgctaacctc gccttacccc ccactattaa + 11881 cctactggga gaactctctg tgctagtaac cacgttctcc tgatcaaata tcactctcct + 11941 acttacagga ctcaacatac tagtcacagc cctatactcc ctctacatat ttaccacaac + 12001 acaatggggc tcactcaccc accacattaa caacataaaa ccctcattca cacgagaaaa + 12061 caccctcatg ttcatacacc tatcccccat tctcctccta tccctcaacc ccgacatcat + 12121 taccgggttt tcctcttgta aatatagttt aaccaaaaca tcagattgtg aatctgacaa + 12181 cagaggctta cgacccctta tttaccgaga aagctcacaa gaactgctaa ctcatgcccc + 12241 catgtctaac aacatggctt tctcaacttt taaaggataa cagctatcca ttggtcttag + 12301 gccccaaaaa ttttggtgca actccaaata aaagtaataa ccatgcacac tactataacc + 12361 accctaaccc tgacttccct aattcccccc atccttacca ccctcgttaa ccctaacaaa + 12421 aaaaactcat acccccatta tgtaaaatcc attgtcgcat ccacctttat tatcagtctc + 12481 ttccccacaa caatattcat gtgcctagac caagaagtta ttatctcgaa ctgacactga + 12541 gccacaaccc aaacaaccca gctctcccta agcttcaaac tagactactt ctccataata + 12601 ttcatccctg tagcattgtt cgttacatgg tccatcatag aattctcact gtgatatata + 12661 aactcagacc caaacattaa tcagttcttc aaatatctac tcatcttcct aattaccata + 12721 ctaatcttag ttaccgctaa caacctattc caactgttca tcggctgaga gggcgtagga + 12781 attatatcct tcttgctcat cagttgatga tacgcccgag cagatgccaa cacagcagcc + 12841 attcaagcaa tcctatacaa ccgtatcggc gatatcggtt tcatcctcgc cttagcatga + 12901 tttatcctac actccaactc atgagaccca caacaaatag cccttctaaa cgctaatcca + 12961 agcctcaccc cactactagg cctcctccta gcagcagcag gcaaatcagc ccaattaggt + 13021 ctccacccct gactcccctc agccatagaa ggccccaccc cagtctcagc cctactccac + 13081 tcaagcacta tagttgtagc aggaatcttc ttactcatcc gcttccaccc cctagcagaa + 13141 aatagcccac taatccaaac tctaacacta tgcttaggcg ctatcaccac tctgttcgca + 13201 gcagtctgcg cccttacaca aaatgacatc aaaaaaatcg tagccttctc cacttcaagt + 13261 caactaggac tcataatagt tacaatcggc atcaaccaac cacacctagc attcctgcac + 13321 atctgtaccc acgccttctt caaagccata ctatttatgt gctccgggtc catcatccac + 13381 aaccttaaca atgaacaaga tattcgaaaa ataggaggac tactcaaaac catacctctc + 13441 acttcaacct ccctcaccat tggcagccta gcattagcag gaataccttt cctcacaggt + 13501 ttctactcca aagaccacat catcgaaacc gcaaacatat catacacaaa cgcctgagcc + 13561 ctatctatta ctctcatcgc tacctccctg acaagcgcct atagcactcg aataattctt + 13621 ctcaccctaa caggtcaacc tcgcttcccc acccttacta acattaacga aaataacccc + 13681 accctactaa accccattaa acgcctggca gccggaagcc tattcgcagg atttctcatt + 13741 actaacaaca tttcccccgc atcccccttc caaacaacaa tccccctcta cctaaaactc + 13801 acagccctcg ctgtcacttt cctaggactt ctaacagccc tagacctcaa ctacctaacc + 13861 aacaaactta aaataaaatc cccactatgc acattttatt tctccaacat actcggattc + 13921 taccctagca tcacacaccg cacaatcccc tatctaggcc ttcttacgag ccaaaacctg + 13981 cccctactcc tcctagacct aacctgacta gaaaagctat tacctaaaac aatttcacag + 14041 caccaaatct ccacctccat catcacctca acccaaaaag gcataattaa actttacttc + 14101 ctctctttct tcttcccact catcctaacc ctactcctaa tcacataacc tattcccccg + 14161 agcaatctca attacaatat atacaccaac aaacaatgtt caaccagtaa ctactactaa + 14221 tcaacgccca taatcataca aagcccccgc accaatagga tcctcccgaa tcaaccctga + 14281 cccctctcct tcataaatta ttcagcttcc tacactatta aagtttacca caaccaccac + 14341 cccatcatac tctttcaccc acagcaccaa tcctacctcc atcgctaacc ccactaaaac + 14401 actcaccaag acctcaaccc ctgaccccca tgcctcagga tactcctcaa tagccatcgc + 14461 tgtagtatat ccaaagacaa ccatcattcc ccctaaataa attaaaaaaa ctattaaacc + 14521 catataacct cccccaaaat tcagaataat aacacacccg accacaccgc taacaatcaa + 14581 tactaaaccc ccataaatag gagaaggctt agaagaaaac cccacaaacc ccattactaa + 14641 acccacactc aacagaaaca aagcatacat cattattctc gcacggacta caaccacgac + 14701 caatgatatg aaaaaccatc gttgtatttc aactacaaga acaccaatga ccccaatacg + 14761 caaaactaac cccctaataa aattaattaa ccactcattc atcgacctcc ccaccccatc + 14821 caacatctcc gcatgatgaa acttcggctc actccttggc gcctgcctga tcctccaaat + 14881 caccacagga ctattcctag ccatgcacta ctcaccagac gcctcaaccg ccttttcatc + 14941 aatcgcccac atcactcgag acgtaaatta tggctgaatc atccgctacc ttcacgccaa + 15001 tggcgcctca atattcttta tctgcctctt cctacacatc gggcgaggcc tatattacgg + 15061 atcatttctc tactcagaaa cctgaaacat cggcattatc ctcctgcttg caactatagc + 15121 aacagccttc ataggctatg tcctcccgtg aggccaaata tcattctgag gggccacagt + 15181 aattacaaac ttactatccg ccatcccata cattgggaca gacctagttc aatgaatctg + 15241 aggaggctac tcagtagaca gtcccaccct cacacgattc tttacctttc acttcatctt + 15301 gcccttcatt attgcagccc tagcaacact ccacctccta ttcttgcacg aaacgggatc + 15361 aaacaacccc ctaggaatca cctcccattc cgataaaatc accttccacc cttactacac + 15421 aatcaaagac gccctcggct tacttctctt ccttctctcc ttaatgacat taacactatt + 15481 ctcaccagac ctcctaggcg acccagacaa ttatacccta gccaacccct taaacacccc + 15541 tccccacatc aagcccgaat gatatttcct attcgcctac acaattctcc gatccgtccc + 15601 taacaaacta ggaggcgtcc ttgccctatt actatccatc ctcatcctag caataatccc + 15661 catcctccat atatccaaac aacaaagcat aatatttcgc ccactaagcc aatcacttta + 15721 ttgactccta gccgcagacc tcctcattct aacctgaatc ggaggacaac cagtaagcta + 15781 cccttttacc atcattggac aagtagcatc cgtactatac ttcacaacaa tcctaatcct + 15841 aataccaact atctccctaa ttgaaaacaa aatactcaaa tgggcctgtc cttgtagtat + 15901 aaactaatac accagtcttg taaaccggag atgaaaacct ttttccaagg acaaatcaga + 15961 gaaaaagtct ttaactccac cattagcacc caaagctaag attctaattt aaactattct + 16021 ctgttctttc atggggaagc agatttgggt accacccaag tattgactca cccatcaaca + 16081 accgctatgt atttcgtaca ttactgccag ccaccatgaa tattgtacgg taccataaat + 16141 acttgaccac ctgtagtaca taaaaaccca atccacatca aaaccccctc cccatgctta + 16201 caagcaagta cagcaatcaa ccctcaacta tcacacatca actgcaactc caaagccacc + 16261 cctcacccac taggatacca acaaacctac ccacccttaa cagtacatag tacataaagc + 16321 catttaccgt acatagcaca ttacagtcaa atcccttctc gtccccatgg atgacccccc + 16381 tcagataggg gtcccttgac caccatcctc cgtgaaatca atatcccgca caagagtgct + 16441 actctcctcg ctccgggccc ataacacttg ggggtagcta aagtgaactg tatccgacat + 16501 ctggttccta cttcagggtc ataaagccta aatagcccac acgttcccct taaataagac + 16561 atcacgatg +// + diff --git a/tests/test_ncbi_process_mito.py b/tests/test_ncbi_process_mito.py new file mode 100644 index 0000000..d0a1982 --- /dev/null +++ b/tests/test_ncbi_process_mito.py @@ -0,0 +1,279 @@ +import os +import unittest +from unittest.mock import MagicMock, patch + +from Bio.SeqRecord import SeqRecord + +from sbin.ncbi_process_mito import ( + download_mito_files, + get_mito_genes, + parse_db_xrefs, + parse_nomenclature_value, +) + +BASE_DIR = os.path.dirname(os.path.abspath(__file__)) + + +class TestNcbiProcessMito(unittest.TestCase): + def verify_mito_gene_attributes(self, mito_gene, expected_values): + for k, v in expected_values.items(): + try: + self.assertEqual(getattr(mito_gene, k), v) + except AssertionError: + print( + f"Test failure on mito gene {mito_gene.gene_symbol} ({mito_gene.gene_id}) " + f'attribute "{k}" with value "{v}" not equal to "{getattr(mito_gene, k)}"' + ) + raise + + @patch("sbin.ncbi_process_mito.download_from_eutils") + def test_download_mito_files(self, mock_download): + output_dir = "test_dir" + accession = "test_accession" + result = download_mito_files(output_dir, accession) + self.assertEqual( + result, + { + "gbff": "test_dir/test_accession.gbff", + "fna": "test_dir/test_accession.fna", + }, + ) + mock_download.assert_any_call(accession, "gb", f"{output_dir}/{accession}.gbff") + mock_download.assert_any_call( + accession, "fasta", f"{output_dir}/{accession}.fna" + ) + + def test_db_xrefs(self): + gb_feature_mock = MagicMock(spec=SeqRecord) + gb_feature_mock.qualifiers = { + "db_xref": ["GeneID:4558", "HGNC:HGNC:7481", "MIM:590070"] + } + + result = parse_db_xrefs(gb_feature_mock) + self.assertEqual( + result, {"GeneID": "4558", "HGNC": "HGNC:7481", "MIM": "590070"} + ) + + def test_db_xrefs_empty(self): + gb_feature_mock = MagicMock(spec=SeqRecord) + gb_feature_mock.qualifiers = {} + + result = parse_db_xrefs(gb_feature_mock) + self.assertEqual(result, {}) + + def test_parse_nomenclature_value(self): + gb_feature_mock = MagicMock(spec=SeqRecord) + gb_feature_mock.qualifiers = { + "nomenclature": [ + "Official Symbol: MT-TF | Name: mitochondrially encoded tRNA phenylalanine | Provided by: HGNC:HGNC:7481" + ] + } + + result = parse_nomenclature_value(gb_feature_mock) + self.assertEqual( + result, + { + "Official Symbol": "MT-TF", + "Name": "mitochondrially encoded tRNA phenylalanine", + "Provided by": "HGNC:HGNC:7481", + }, + ) + + def test_parse_nomenclature_value_empty(self): + gb_feature_mock = MagicMock(spec=SeqRecord) + gb_feature_mock.qualifiers = {} + + result = parse_nomenclature_value(gb_feature_mock) + self.assertEqual(result, {}) + + def test_get_mito_genes(self): + mito_genbank_filepath = f"{BASE_DIR}/data/NC_012920.1.gbff" + results = [_ for _ in get_mito_genes(mito_genbank_filepath)] + expected_gene_ids = [ + 4508, + 4509, + 4511, + 4512, + 4513, + 4514, + 4519, + 4535, + 4536, + 4537, + 4538, + 4539, + 4540, + 4541, + 4549, + 4550, + 4553, + 4555, + 4556, + 4558, + 4563, + 4564, + 4565, + 4566, + 4567, + 4568, + 4569, + 4570, + 4571, + 4572, + 4573, + 4574, + 4575, + 4576, + 4577, + 4578, + 4579, + ] + expected_gene_symbols = [ + "MT-ATP6", + "MT-ATP8", + "MT-CO1", + "MT-CO2", + "MT-CO3", + "MT-CYB", + "MT-ND1", + "MT-ND2", + "MT-ND3", + "MT-ND4", + "MT-ND4L", + "MT-ND5", + "MT-ND6", + "MT-RNR1", + "MT-RNR2", + "MT-TA", + "MT-TC", + "MT-TD", + "MT-TE", + "MT-TF", + "MT-TG", + "MT-TH", + "MT-TI", + "MT-TK", + "MT-TL1", + "MT-TL2", + "MT-TM", + "MT-TN", + "MT-TP", + "MT-TQ", + "MT-TR", + "MT-TS1", + "MT-TS2", + "MT-TT", + "MT-TV", + "MT-TW", + "MT-TY", + ] + expected_origin = "NCBI" + expected_aln_method = "splign" + + self.assertEqual(len(results), 37) + self.assertEqual(sorted([r.gene_id for r in results]), expected_gene_ids) + self.assertEqual( + sorted([r.gene_symbol for r in results]), expected_gene_symbols + ) + self.assertEqual([r.origin for r in results], [expected_origin] * 37) + self.assertEqual( + [r.alignment_method for r in results], [expected_aln_method] * 37 + ) + + results_by_gene = {mg.gene_id: mg for mg in results} + + # Expected results for "MT-TV" non-coding tRNA gene on the plus strand + expected_mg4577_values = { + "gene_symbol": "MT-TV", + "name": "mitochondrially encoded tRNA valine", + "tx_ac": "NC_012920.1_01601_01670", + "tx_seq": "CAGAGTGTAGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGA", + "tx_start": 0, + "tx_end": 69, + "alt_ac": "NC_012920.1", + "alt_start": 1601, + "alt_end": 1670, + "strand": "+", + "transl_table": None, + "transl_except": None, + "pro_ac": None, + "pro_seq": None, + } + self.verify_mito_gene_attributes(results_by_gene[4577], expected_mg4577_values) + + # Expected results for "MT-TQ" tRNA gene on the minus strand + expected_mg4572_values = { + "gene_symbol": "MT-TQ", + "name": "mitochondrially encoded tRNA glutamine", + "tx_ac": "NC_012920.1_04328_04400", + "tx_seq": "TAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGGGATGGGTTCGATTCTCATAGTCCTAG", + "tx_start": 0, + "tx_end": 72, + "alt_ac": "NC_012920.1", + "alt_start": 4328, + "alt_end": 4400, + "strand": "-", + "transl_table": None, + "transl_except": None, + "pro_ac": None, + "pro_seq": None, + } + self.verify_mito_gene_attributes(results_by_gene[4572], expected_mg4572_values) + + # Expected results for "MT-CO2" coding gene on the plus strand + expected_mg4513_values = { + "gene_symbol": "MT-CO2", + "name": "mitochondrially encoded cytochrome c oxidase II", + "tx_ac": "NC_012920.1_07585_08269", + "tx_seq": "ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT" + "TCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACT" + "ATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGG" + "CCACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC" + "TTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTA" + "AAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAG" + "TTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG", + "tx_start": 0, + "tx_end": 684, + "alt_ac": "NC_012920.1", + "alt_start": 7585, + "alt_end": 8269, + "strand": "+", + "transl_table": "2", + "transl_except": None, + "pro_ac": "YP_003024029.1", + "pro_seq": "MAHAAQVGLQDATSPIMEELITFHDHALMIIFLICFLVLYALFLTLTTKLTNTNISDAQEMETVWTILPAIILVLIALPSLRILYMTDEVNDP" + "SLTIKSIGHQWYWTYEYTDYGGLIFNSYMLPPLFLEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVLHSWAVPTLGLKTDAIPGRLNQTTFTATRPGVYYGQCS" + "EICGANHSFMPIVLELIPLKIFEMGPVFTL", + } + self.verify_mito_gene_attributes(results_by_gene[4513], expected_mg4513_values) + + # Expected results for "MT-ND1" coding gene on the minus strand with a transl_except + expected_mg4535_values = { + "gene_symbol": "MT-ND1", + "name": "mitochondrially encoded NADH dehydrogenase 1", + "tx_ac": "NC_012920.1_03306_04262", + "tx_seq": "ATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATAC" + "AACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGCCACATCT" + "ACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACCATCGCTCTTCTACTATGAACCCCCCTCCCCATACCCAACCCCCTGGTCAACCTCAACCTAGG" + "CCTCCTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCGCACTGCGAGCAG" + "TAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCTACTATCAACATTACTAATAAGTGGCTCCTTTAACCTCTCCACCCTTATCACAACACAAGAA" + "CACCTCTGATTACTCCTGCCATCATGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTC" + "CGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCGAATACACAAACATTATTATAATAAACACCCTCACCACTA" + "CAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCTTATGAATTCGA" + "ACAGCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACCCAT" + "TACAATCTCCAGCATTCCCCCTCAAACCTA", + "tx_start": 0, + "tx_end": 956, + "alt_ac": "NC_012920.1", + "alt_start": 3306, + "alt_end": 4262, + "strand": "+", + "transl_table": "2", + "transl_except": "(pos:4261..4262,aa:TERM)", + "pro_ac": "YP_003024026.1", + "pro_seq": "MPMANLLLLIVPILIAMAFLMLTERKILGYMQLRKGPNVVGPYGLLQPFADAMKLFTKEPLKPATSTITLYITAPTLALTIALLLWTPLPMPN" + "PLVNLNLGLLFILATSSLAVYSILWSGWASNSNYALIGALRAVAQTISYEVTLAIILLSTLLMSGSFNLSTLITTQEHLWLLLPSWPLAMMWFISTLAETNRTP" + "FDLAEGESELVSGFNIEYAAGPFALFFMAEYTNIIMMNTLTTTIFLGTTYDALSPELYTTYFVTKTLLLTSLFLWIRTAYPRFRYDQLMHLLWKNFLPLTLALL" + "MWYVSMPITISSIPPQT", + } + self.verify_mito_gene_attributes(results_by_gene[4535], expected_mg4535_values) From c5c1918b32ef2af16e92c068eddece960f64cf13 Mon Sep 17 00:00:00 2001 From: nvta1209 <162694616+nvta1209@users.noreply.github.com> Date: Mon, 1 Apr 2024 13:10:31 -0700 Subject: [PATCH 26/54] feat(IPVC-2265): associated_accessions (#15) --- README.md | 2 +- etc/scripts/run-uta-build.sh | 6 ++ sbin/ncbi-parse-gene2refseq | 4 +- src/uta/cli.py | 7 +-- src/uta/exceptions.py | 1 + src/uta/formats/geneinfo.py | 1 - src/uta/loading.py | 81 ++++++++++++++++++++++++-- src/uta/models.py | 22 +++++++ tests/data/assocacs.gz | Bin 0 -> 144 bytes tests/test_uta_loading.py | 108 +++++++++++++++++++++++++++++++++++ tests/test_uta_models.py | 49 ++++++++++++++-- 11 files changed, 262 insertions(+), 19 deletions(-) create mode 100644 tests/data/assocacs.gz create mode 100644 tests/test_uta_loading.py diff --git a/README.md b/README.md index e897474..8b95314 100644 --- a/README.md +++ b/README.md @@ -289,7 +289,7 @@ To develop UTA, follow these steps. 4. Testing $ docker build --target uta-test -t uta-test . - $ docker run -it --rm -v $(pwd)/tests:/opt/repos/uta/tests -v $(pwd)/src:/opt/repos/uta/src uta-test bash + $ docker run -it --rm -v $(pwd):/opt/repos/uta uta-test bash $ python -m unittest ## UTA update procedure diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh index 86175c8..166c6cb 100755 --- a/etc/scripts/run-uta-build.sh +++ b/etc/scripts/run-uta-build.sh @@ -47,6 +47,9 @@ sbin/ncbi-parse-geneinfo $ncbi_dir/gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gen sbin/ncbi-parse-gene2refseq $ncbi_dir/gene/DATA/gene2accession.gz | gzip -c > "$loading_dir/assocacs.gz" 2>&1 | \ tee "$logs_dir/ncbi-fetch-assoc-acs" +sbin/assoc-acs-merge "$loading_dir/assocacs.gz" | gzip -c > "$loading_dir/assocacs.cleaned.gz" 2>&1 | \ + tee "$logs_dir/assoc-acs-merge" + # parse transcript info from GBFF input files GBFF_files=$(ls $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz) sbin/ncbi-parse-gbff "$GBFF_files" | gzip -c > "$loading_dir/gbff.txinfo.gz" 2>&1 | \ @@ -66,6 +69,9 @@ sbin/exonset-to-seqinfo -o NCBI "$loading_dir/gff.exonsets.gz" | gzip -c > "$loa uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$loading_dir/genes.geneinfo.gz" 2>&1 | \ tee "$logs_dir/load-geneinfo.log" +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-assoc-ac "$loading_dir/assocacs.cleaned.gz" 2>&1 | \ + tee "$logs_dir/load-assoc-ac.log" + # transcript info uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$loading_dir/gbff.txinfo.gz" 2>&1 | \ tee "$logs_dir/load-txinfo.log" diff --git a/sbin/ncbi-parse-gene2refseq b/sbin/ncbi-parse-gene2refseq index 7bfe70c..1f2f6cb 100755 --- a/sbin/ncbi-parse-gene2refseq +++ b/sbin/ncbi-parse-gene2refseq @@ -10,8 +10,6 @@ ftp://ftp.ncbi.nih.gov/gene/DATA/gene2refseq.gz import io import sys -from csv import DictReader - from uta.formats.geneaccessions import GeneAccessions, GeneAccessionsWriter from uta.formats.ncbitsv import NCBITSVReader @@ -47,7 +45,7 @@ if __name__ == "__main__": key = (ga.gene_symbol, ga.tx_ac, ga.gene_id, ga.pro_ac) if key in seen: - continue + continue seen.add(key) gaw.write(ga) diff --git a/src/uta/cli.py b/src/uta/cli.py index 4b85440..adc8e8b 100644 --- a/src/uta/cli.py +++ b/src/uta/cli.py @@ -13,13 +13,14 @@ uta (-C CONF ...) [options] load-geneinfo FILE uta (-C CONF ...) [options] load-txinfo FILE uta (-C CONF ...) [options] load-exonset FILE + uta (-C CONF ...) [options] load-assoc-ac FILE uta (-C CONF ...) [options] load-sequences uta (-C CONF ...) [options] align-exons [--sql SQL] uta (-C CONF ...) [options] load-ncbi-seqgene FILE uta (-C CONF ...) [options] grant-permissions uta (-C CONF ...) [options] refresh-matviews uta (-C CONF ...) [options] analyze - + Options: -C CONF, --conf CONF Configuration to read (required) @@ -69,6 +70,7 @@ def main(): ("create-schema", ul.create_schema), ("drop-schema", ul.drop_schema), ("grant-permissions", ul.grant_permissions), + ("load-assoc-ac", ul.load_assoc_ac), ("load-exonset", ul.load_exonset), ("load-geneinfo", ul.load_geneinfo), ("load-origin", ul.load_origin), @@ -118,13 +120,10 @@ def main(): cmd=cmd, elapsed=time.time() - t0)) - if __name__ == "__main__": main() - - # # Copyright 2014 UTA Contributors (https://bitbucket.org/biocommons/uta) ## diff --git a/src/uta/exceptions.py b/src/uta/exceptions.py index a0cad0c..e391297 100644 --- a/src/uta/exceptions.py +++ b/src/uta/exceptions.py @@ -21,6 +21,7 @@ class InvalidHGVSVariantError(UTAError): class EutilsDownloadError(Exception): pass + # # Copyright 2014 UTA Contributors (https://bitbucket.org/biocommons/uta) ## diff --git a/src/uta/formats/geneinfo.py b/src/uta/formats/geneinfo.py index c226452..e094adf 100644 --- a/src/uta/formats/geneinfo.py +++ b/src/uta/formats/geneinfo.py @@ -38,7 +38,6 @@ def __next__(self): return GeneInfo(**d) - if __name__ == '__main__': tmpfn = '/tmp/exonset' diff --git a/src/uta/loading.py b/src/uta/loading.py index 7d1c310..7ddd354 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -7,12 +7,14 @@ import itertools import logging import time +from typing import Any from biocommons.seqrepo import SeqRepo from bioutils.coordinates import strand_pm_to_int, MINUS_STRAND from bioutils.digests import seq_md5 from bioutils.sequences import reverse_complement from sqlalchemy.exc import IntegrityError +from sqlalchemy.orm import Session from sqlalchemy.orm.exc import NoResultFound from sqlalchemy import text import psycopg2.extras @@ -240,6 +242,47 @@ def grant_permissions(session, opts, cf): session.commit() +def load_assoc_ac(session, opts, cf): + """ + Insert rows into `associated_accessions` table in the UTA database, + using data from a file written by sbin/assoc-acs-merge. + """ + logger.info("load_assoc_ac") + + admin_role = cf.get("uta", "admin_role") + session.execute(text(f"set role {admin_role};")) + session.execute(text(f"set search_path = {usam.schema_name};")) + fname = opts["FILE"] + + with gzip.open(fname, "rt") as fhandle: + for file_row in csv.DictReader(fhandle, delimiter="\t"): + row = { + "origin": file_row["origin"], + "pro_ac": file_row["pro_ac"], + "tx_ac": file_row["tx_ac"], + } + aa, created = _get_or_insert( + session=session, + table=usam.AssociatedAccessions, + row=row, + row_identifier=('origin', 'tx_ac', 'pro_ac'), + ) + if created: + # If committing on every insert is too slow, we can + # look into committing in batches like load_txinfo does. + session.commit() + logger.info(f"Added: {aa.tx_ac}, {aa.pro_ac}, {aa.origin}") + else: + logger.info(f"Already exists: {file_row}") + # All fields should should match when unique identifiers match. + # Discrepancies should be investigated. + existing_row = { + "origin": aa.origin, + "pro_ac": aa.pro_ac, + "tx_ac": aa.tx_ac, + } + + def load_exonset(session, opts, cf): # exonsets and associated exons are loaded together @@ -265,7 +308,7 @@ def load_exonset(session, opts, cf): logger.exception(e) session.rollback() n_errors += 1 - finally: + finally: (no) = (n is not None, o is not None) if no == (True, False): n_new += 1 @@ -305,7 +348,7 @@ def load_geneinfo(session, opts, cf): def load_ncbi_geneinfo(session, opts, cf): """ - import data as downloaded (by you) from + import data as downloaded (by you) from ftp://ftp.ncbi.nlm.nih.gov/gene/DATA/gene_info.gz """ @@ -475,7 +518,7 @@ def _upsert_seq(si): for md5, si_iter in itertools.groupby(sorted(sir, key=lambda si: si.md5), key=lambda si: si.md5): sis = list(si_iter) - + # if sequence doesn't exist in sequence table, make it # this is to satisfy a FK dependency, which should be reconsidered si = sis[0] @@ -688,8 +731,6 @@ def _fetch_origin_by_name(name): i_ti=i_ti, n_rows=n_rows, n_new=n_new, n_unchanged=n_unchanged, n_cds_changed=n_cds_changed, n_exons_changed=n_exons_changed, p=(i_ti + 1) / n_rows * 100)) - - def refresh_matviews(session, opts, cf): @@ -740,6 +781,34 @@ def _get_seqrepo(cf): _get_seqfetcher = _get_seqrepo +def _get_or_insert( + session: Session, + table: type[usam.Base], + row: dict[str, Any], + row_identifier: str | tuple[str, ...], +) -> tuple[usam.Base, bool]: + """ + Returns a sqlalchemy model of the inserted or fetched row. + + `session` is a sqlalchemy session. + `table` is the database table in which to insert `row`. + `row` is the a list of key-value pairs to insert into the table. + `row_identifier` is a map of key-value pairs which define a match between `row` and an existing row in the table. + + sqlalchemy.orm.exc.MultipleResultsFound may be raised if `row_identifier` does not uniquely identify a row. + KeyError may be raised if `row_identifier` refers to columns not present as keys in `row`. + sqlalchemy.exc.IntegrityError (raised from psycopg2.errors.ForeignKeyViolation) may be raised if a foreign key reference does not exist + """ + row_filter = {ri: row[ri] for ri in row_identifier} + try: + row_instance = session.query(table).filter_by(**row_filter).one() + created = False + except NoResultFound: + row_instance = table(**row) + session.add(row_instance) + created = True + return row_instance, created + def _upsert_exon_set_record(session, tx_ac, alt_ac, strand, method, ess): @@ -749,7 +818,7 @@ def _upsert_exon_set_record(session, tx_ac, alt_ac, strand, method, ess): (new, None) -- no prior record; new was inserted (None, old) -- prior record and unchaged; nothing was inserted (new, old) -- prior record existed and was changed - + """ key = (tx_ac, alt_ac, method) diff --git a/src/uta/models.py b/src/uta/models.py index 2305666..20c71b5 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -6,6 +6,8 @@ import sqlalchemy as sa import sqlalchemy.orm as sao +import sqlalchemy.types +import sqlalchemy.sql.functions from sqlalchemy.ext.declarative import declarative_base @@ -220,6 +222,26 @@ class ExonAln(Base): # methods: +class AssociatedAccessions(Base): + __tablename__ = "associated_accessions" + __table_args__ = ( + sa.UniqueConstraint("origin", "tx_ac", "pro_ac", name="unique_pair_in_origin"), + sa.Index("associated_accessions_pro_ac", "pro_ac"), + sa.Index("associated_accessions_tx_ac", "tx_ac"), + ) + + # columns: + associated_accession_id = sa.Column(sa.Integer, primary_key=True, autoincrement=True) + tx_ac = sa.Column(sa.Text, nullable=False) + pro_ac = sa.Column(sa.Text, nullable=False) + origin = sa.Column(sa.Text, nullable=False) + added = sa.Column( + sqlalchemy.types.TIMESTAMP, + server_default=sqlalchemy.sql.functions.now(), + nullable=False, + ) + + # # Copyright 2014 UTA Contributors (https://bitbucket.org/biocommons/uta) ## diff --git a/tests/data/assocacs.gz b/tests/data/assocacs.gz new file mode 100644 index 0000000000000000000000000000000000000000..10214c22b780f85aff6da9cb855410b5b5462d90 GIT binary patch literal 144 zcmV;B0B`>viwFn`xcg-Q17UM>Z)0I&a{x Date: Tue, 2 Apr 2024 12:33:42 -0700 Subject: [PATCH 27/54] feat(IPVC-2299): Run tests in a github action (#17) --- .github/workflows/test.yml | 25 +++++++++++++++++++++++++ Dockerfile | 5 +++-- README.md | 3 +-- 3 files changed, 29 insertions(+), 4 deletions(-) create mode 100644 .github/workflows/test.yml diff --git a/.github/workflows/test.yml b/.github/workflows/test.yml new file mode 100644 index 0000000..409225a --- /dev/null +++ b/.github/workflows/test.yml @@ -0,0 +1,25 @@ +name: Continuous Integration + +on: + push: + branches: + - main + pull_request: + branches: + - main + merge_group: + types: + - checks_requested + +jobs: + test: + name: Run tests + runs-on: ubuntu-latest + timeout-minutes: 10 + steps: + - name: Checkout + uses: actions/checkout@v3 + - name: Build image + run: docker build --target uta-test -t uta-test . + - name: Run tests + run: docker run --rm uta-test python -m unittest diff --git a/Dockerfile b/Dockerfile index ec6d67d..e58df99 100644 --- a/Dockerfile +++ b/Dockerfile @@ -27,5 +27,6 @@ FROM uta as uta-test RUN DEBIAN_FRONTEND=noninteractive apt-get -yq install postgresql COPY tests ./tests RUN pip install -e .[test] -RUN useradd postgres-testing -USER postgres-testing +RUN useradd uta-tester +RUN chown -R uta-tester . +USER uta-tester diff --git a/README.md b/README.md index 8b95314..78da019 100644 --- a/README.md +++ b/README.md @@ -289,8 +289,7 @@ To develop UTA, follow these steps. 4. Testing $ docker build --target uta-test -t uta-test . - $ docker run -it --rm -v $(pwd):/opt/repos/uta uta-test bash - $ python -m unittest + $ docker run -it --rm uta-test python -m unittest ## UTA update procedure From 2111c42fd5d16e5b6e97ea91d5f9ec0304ae6014 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Fri, 5 Apr 2024 11:32:32 -0600 Subject: [PATCH 28/54] feat(IPVC-2323): add Alembic schema migrations (#19) --- etc/alembic.ini | 116 ++++++ etc/scripts/create-new-schema.sh | 1 + etc/scripts/run-uta-build.sh | 6 +- pyproject.toml | 1 + src/alembic/README | 1 + src/alembic/env.py | 92 +++++ src/alembic/script.py.mako | 26 ++ ...ae896_add_sqlalchemy_model_for_assocacs.py | 44 ++ .../versions/edadb97f6502_initial_state.py | 386 ++++++++++++++++++ src/uta/models.py | 14 +- 10 files changed, 679 insertions(+), 8 deletions(-) create mode 100644 etc/alembic.ini create mode 100644 src/alembic/README create mode 100644 src/alembic/env.py create mode 100644 src/alembic/script.py.mako create mode 100644 src/alembic/versions/cc51f50ae896_add_sqlalchemy_model_for_assocacs.py create mode 100644 src/alembic/versions/edadb97f6502_initial_state.py diff --git a/etc/alembic.ini b/etc/alembic.ini new file mode 100644 index 0000000..6777380 --- /dev/null +++ b/etc/alembic.ini @@ -0,0 +1,116 @@ +# A generic, single database configuration. + +[alembic] +# path to migration scripts +script_location = src/alembic + +# template used to generate migration file names; The default value is %%(rev)s_%%(slug)s +# Uncomment the line below if you want the files to be prepended with date and time +# see https://alembic.sqlalchemy.org/en/latest/tutorial.html#editing-the-ini-file +# for all available tokens +# file_template = %%(year)d_%%(month).2d_%%(day).2d_%%(hour).2d%%(minute).2d-%%(rev)s_%%(slug)s + +# sys.path path, will be prepended to sys.path if present. +# defaults to the current working directory. +prepend_sys_path = . + +# timezone to use when rendering the date within the migration file +# as well as the filename. +# If specified, requires the python>=3.9 or backports.zoneinfo library. +# Any required deps can installed by adding `alembic[tz]` to the pip requirements +# string value is passed to ZoneInfo() +# leave blank for localtime +# timezone = + +# max length of characters to apply to the +# "slug" field +# truncate_slug_length = 40 + +# set to 'true' to run the environment during +# the 'revision' command, regardless of autogenerate +# revision_environment = false + +# set to 'true' to allow .pyc and .pyo files without +# a source .py file to be detected as revisions in the +# versions/ directory +# sourceless = false + +# version location specification; This defaults +# to alembic/versions. When using multiple version +# directories, initial revisions must be specified with --version-path. +# The path separator used here should be the separator specified by "version_path_separator" below. +# version_locations = %(here)s/bar:%(here)s/bat:alembic/versions + +# version path separator; As mentioned above, this is the character used to split +# version_locations. The default within new alembic.ini files is "os", which uses os.pathsep. +# If this key is omitted entirely, it falls back to the legacy behavior of splitting on spaces and/or commas. +# Valid values for version_path_separator are: +# +# version_path_separator = : +# version_path_separator = ; +# version_path_separator = space +version_path_separator = os # Use os.pathsep. Default configuration used for new projects. + +# set to 'true' to search source files recursively +# in each "version_locations" directory +# new in Alembic version 1.10 +# recursive_version_locations = false + +# the output encoding used when revision files +# are written from script.py.mako +# output_encoding = utf-8 + +sqlalchemy.url = postgresql://uta_admin:@localhost/uta + + +[post_write_hooks] +# post_write_hooks defines scripts or Python functions that are run +# on newly generated revision scripts. See the documentation for further +# detail and examples + +# format using "black" - use the console_scripts runner, against the "black" entrypoint +# hooks = black +# black.type = console_scripts +# black.entrypoint = black +# black.options = -l 79 REVISION_SCRIPT_FILENAME + +# lint with attempts to fix using "ruff" - use the exec runner, execute a binary +# hooks = ruff +# ruff.type = exec +# ruff.executable = %(here)s/.venv/bin/ruff +# ruff.options = --fix REVISION_SCRIPT_FILENAME + +# Logging configuration +[loggers] +keys = root,sqlalchemy,alembic + +[handlers] +keys = console + +[formatters] +keys = generic + +[logger_root] +level = WARN +handlers = console +qualname = + +[logger_sqlalchemy] +level = WARN +handlers = +qualname = sqlalchemy.engine + +[logger_alembic] +level = INFO +handlers = +qualname = alembic + +[handler_console] +class = StreamHandler +args = (sys.stderr,) +level = NOTSET +formatter = generic + +[formatter_generic] +format = %(levelname)-5.5s [%(name)s] %(message)s +datefmt = %H:%M:%S diff --git a/etc/scripts/create-new-schema.sh b/etc/scripts/create-new-schema.sh index 0b6aa2b..5200e8d 100755 --- a/etc/scripts/create-new-schema.sh +++ b/etc/scripts/create-new-schema.sh @@ -21,4 +21,5 @@ pg_dump -U uta_admin -h localhost -d uta -n "$source_uta_v" | \ # create new schema gzip -cdq $dumps_dir/"$source_uta_v".pgd.gz | \ sbin/pg-dump-schema-rename "$source_uta_v" "$dest_uta_v" | \ + sbin/pg-dump-schema-rename "uta_1_1" "$dest_uta_v" | \ psql -U uta_admin -h localhost -d uta -aeE \ No newline at end of file diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh index 166c6cb..f4dec9a 100755 --- a/etc/scripts/run-uta-build.sh +++ b/etc/scripts/run-uta-build.sh @@ -19,7 +19,7 @@ then fi # set local variables and create working directories -loading_uta_v="uta_1_1" +loading_uta_v="uta" loading_dir="$working_dir/loading" dumps_dir="$working_dir/dumps" logs_dir="$working_dir/logs" @@ -31,6 +31,10 @@ done etc/scripts/delete-schema.sh "$loading_uta_v" etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_uta_v" +## for now set up Alembic for schema migrations +alembic -c etc/alembic.ini stamp edadb97f6502 +alembic -c etc/alembic.ini upgrade head + ## Load SeqRepo with new sequences seqrepo load -n NCBI -i "$seqrepo_data_release" \ $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz \ diff --git a/pyproject.toml b/pyproject.toml index 5b6b4a2..652c22d 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -39,6 +39,7 @@ classifiers = [ ] dependencies = [ + "alembic", "attrs", "biocommons.seqrepo", "biopython>=1.69", diff --git a/src/alembic/README b/src/alembic/README new file mode 100644 index 0000000..98e4f9c --- /dev/null +++ b/src/alembic/README @@ -0,0 +1 @@ +Generic single-database configuration. \ No newline at end of file diff --git a/src/alembic/env.py b/src/alembic/env.py new file mode 100644 index 0000000..1e8e830 --- /dev/null +++ b/src/alembic/env.py @@ -0,0 +1,92 @@ +from logging.config import fileConfig + +from sqlalchemy import engine_from_config +from sqlalchemy import pool + +from alembic import context + +# this is the Alembic Config object, which provides +# access to the values within the .ini file in use. +config = context.config + +# Interpret the config file for Python logging. +# This line sets up loggers basically. +if config.config_file_name is not None: + fileConfig(config.config_file_name) + +# add your model's MetaData object here +# for 'autogenerate' support +# from myapp import mymodel +# target_metadata = mymodel.Base.metadata +from uta.models import Base +target_metadata = Base.metadata + +# other values from the config, defined by the needs of env.py, +# can be acquired: +# my_important_option = config.get_main_option("my_important_option") +# ... etc. + + +def include_name(name, type_, parent_names) -> bool: + if type_ == "schema": + return name in ["uta"] + else: + return True + + +def run_migrations_offline() -> None: + """Run migrations in 'offline' mode. + + This configures the context with just a URL + and not an Engine, though an Engine is acceptable + here as well. By skipping the Engine creation + we don't even need a DBAPI to be available. + + Calls to context.execute() here emit the given string to the + script output. + + """ + url = config.get_main_option("sqlalchemy.url") + context.configure( + url=url, + target_metadata=target_metadata, + literal_binds=True, + dialect_opts={"paramstyle": "named"}, + ) + + with context.begin_transaction(): + context.run_migrations() + + +def run_migrations_online() -> None: + """Run migrations in 'online' mode. + + In this scenario we need to create an Engine + and associate a connection with the context. + + """ + connectable = engine_from_config( + config.get_section(config.config_ini_section, {}), + prefix="sqlalchemy.", + poolclass=pool.NullPool, + ) + + with connectable.connect() as connection: + context.configure( + connection=connection, + target_metadata=target_metadata, + version_table_schema=target_metadata.schema, + include_schemas=True, + include_name=include_name, + ) + + with context.begin_transaction(): + context.execute(f'create schema if not exists {target_metadata.schema};') + context.execute(f'set search_path to {target_metadata.schema}') + context.run_migrations() + + +if context.is_offline_mode(): + run_migrations_offline() +else: + run_migrations_online() diff --git a/src/alembic/script.py.mako b/src/alembic/script.py.mako new file mode 100644 index 0000000..fbc4b07 --- /dev/null +++ b/src/alembic/script.py.mako @@ -0,0 +1,26 @@ +"""${message} + +Revision ID: ${up_revision} +Revises: ${down_revision | comma,n} +Create Date: ${create_date} + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa +${imports if imports else ""} + +# revision identifiers, used by Alembic. +revision: str = ${repr(up_revision)} +down_revision: Union[str, None] = ${repr(down_revision)} +branch_labels: Union[str, Sequence[str], None] = ${repr(branch_labels)} +depends_on: Union[str, Sequence[str], None] = ${repr(depends_on)} + + +def upgrade() -> None: + ${upgrades if upgrades else "pass"} + + +def downgrade() -> None: + ${downgrades if downgrades else "pass"} diff --git a/src/alembic/versions/cc51f50ae896_add_sqlalchemy_model_for_assocacs.py b/src/alembic/versions/cc51f50ae896_add_sqlalchemy_model_for_assocacs.py new file mode 100644 index 0000000..c8ee756 --- /dev/null +++ b/src/alembic/versions/cc51f50ae896_add_sqlalchemy_model_for_assocacs.py @@ -0,0 +1,44 @@ +"""add sqlalchemy model for assocacs + +Revision ID: cc51f50ae896 +Revises: edadb97f6502 +Create Date: 2024-04-05 00:33:40.105587 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa + + +# revision identifiers, used by Alembic. +revision: str = 'cc51f50ae896' +down_revision: Union[str, None] = 'edadb97f6502' +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.alter_column('associated_accessions', 'tx_ac', + existing_type=sa.TEXT(), + nullable=False, + schema='uta') + op.alter_column('associated_accessions', 'pro_ac', + existing_type=sa.TEXT(), + nullable=False, + schema='uta') + # ### end Alembic commands ### + + +def downgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.alter_column('associated_accessions', 'pro_ac', + existing_type=sa.TEXT(), + nullable=True, + schema='uta') + op.alter_column('associated_accessions', 'tx_ac', + existing_type=sa.TEXT(), + nullable=True, + schema='uta') + # ### end Alembic commands ### diff --git a/src/alembic/versions/edadb97f6502_initial_state.py b/src/alembic/versions/edadb97f6502_initial_state.py new file mode 100644 index 0000000..86ea9cd --- /dev/null +++ b/src/alembic/versions/edadb97f6502_initial_state.py @@ -0,0 +1,386 @@ +"""initial state + +Revision ID: edadb97f6502 +Revises: +Create Date: 2024-04-03 21:41:05.875580 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa +from sqlalchemy.dialects import postgresql + + +# revision identifiers, used by Alembic. +revision: str = 'edadb97f6502' +down_revision: Union[str, None] = None +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.create_table('gene', + sa.Column('hgnc', sa.Text(), nullable=False), + sa.Column('maploc', sa.Text(), nullable=True), + sa.Column('descr', sa.Text(), nullable=True), + sa.Column('summary', sa.Text(), nullable=True), + sa.Column('aliases', sa.Text(), nullable=True), + sa.Column('added', sa.DateTime(), nullable=False), + sa.PrimaryKeyConstraint('hgnc'), + schema='uta' + ) + op.create_table('meta', + sa.Column('key', sa.Text(), nullable=False), + sa.Column('value', sa.Text(), nullable=False), + sa.PrimaryKeyConstraint('key'), + schema='uta' + ) + op.create_table('origin', + sa.Column('origin_id', sa.Integer(), autoincrement=True, nullable=False), + sa.Column('name', sa.Text(), nullable=False), + sa.Column('descr', sa.Text(), nullable=True), + sa.Column('updated', sa.DateTime(), nullable=True), + sa.Column('url', sa.Text(), nullable=True), + sa.Column('url_ac_fmt', sa.Text(), nullable=True), + sa.PrimaryKeyConstraint('origin_id'), + sa.UniqueConstraint('name'), + schema='uta' + ) + op.create_table('seq', + sa.Column('seq_id', sa.Text(), nullable=False), + sa.Column('len', sa.Integer(), nullable=False), + sa.Column('seq', sa.Text(), nullable=True), + sa.PrimaryKeyConstraint('seq_id'), + schema='uta' + ) + op.create_table('seq_anno', + sa.Column('seq_anno_id', sa.Integer(), autoincrement=True, nullable=False), + sa.Column('seq_id', sa.Text(), nullable=True), + sa.Column('origin_id', sa.Integer(), nullable=False), + sa.Column('ac', sa.Text(), nullable=False), + sa.Column('descr', sa.Text(), nullable=True), + sa.Column('added', sa.DateTime(), nullable=False), + sa.ForeignKeyConstraint(['origin_id'], ['uta.origin.origin_id'], onupdate='CASCADE', ondelete='CASCADE'), + sa.ForeignKeyConstraint(['seq_id'], ['uta.seq.seq_id'], onupdate='CASCADE', ondelete='CASCADE'), + sa.PrimaryKeyConstraint('seq_anno_id'), + schema='uta' + ) + op.create_index(op.f('ix_uta_seq_anno_ac'), 'seq_anno', ['ac'], unique=False, schema='uta') + op.create_index(op.f('ix_uta_seq_anno_seq_id'), 'seq_anno', ['seq_id'], unique=False, schema='uta') + op.create_index('seq_anno_ac_unique_in_origin', 'seq_anno', ['origin_id', 'ac'], unique=True, schema='uta') + op.create_table('transcript', + sa.Column('ac', sa.Text(), nullable=False), + sa.Column('origin_id', sa.Integer(), nullable=False), + sa.Column('hgnc', sa.Text(), nullable=True), + sa.Column('cds_start_i', sa.Integer(), nullable=True), + sa.Column('cds_end_i', sa.Integer(), nullable=True), + sa.Column('cds_md5', sa.Text(), nullable=True), + sa.Column('added', sa.DateTime(), nullable=False), + sa.CheckConstraint('cds_start_i <= cds_end_i', name='cds_start_i_must_be_le_cds_end_i'), + sa.ForeignKeyConstraint(['origin_id'], ['uta.origin.origin_id'], onupdate='CASCADE', ondelete='CASCADE'), + sa.PrimaryKeyConstraint('ac'), + schema='uta' + ) + op.create_index(op.f('ix_uta_transcript_cds_md5'), 'transcript', ['cds_md5'], unique=False, schema='uta') + op.create_index(op.f('ix_uta_transcript_origin_id'), 'transcript', ['origin_id'], unique=False, schema='uta') + op.create_table('exon_set', + sa.Column('exon_set_id', sa.Integer(), autoincrement=True, nullable=False), + sa.Column('tx_ac', sa.Text(), nullable=False), + sa.Column('alt_ac', sa.Text(), nullable=False), + sa.Column('alt_strand', sa.SmallInteger(), nullable=False), + sa.Column('alt_aln_method', sa.Text(), nullable=False), + sa.Column('added', sa.DateTime(), nullable=False), + sa.ForeignKeyConstraint(['tx_ac'], ['uta.transcript.ac'], onupdate='CASCADE', ondelete='CASCADE'), + sa.PrimaryKeyConstraint('exon_set_id'), + sa.UniqueConstraint('tx_ac', 'alt_ac', 'alt_aln_method', name=' must be unique'), + schema='uta' + ) + op.create_table('exon', + sa.Column('exon_id', sa.Integer(), autoincrement=True, nullable=False), + sa.Column('exon_set_id', sa.Integer(), nullable=False), + sa.Column('start_i', sa.Integer(), nullable=False), + sa.Column('end_i', sa.Integer(), nullable=False), + sa.Column('ord', sa.Integer(), nullable=False), + sa.Column('name', sa.Text(), nullable=True), + sa.CheckConstraint('start_i < end_i', name='exon_start_i_must_be_lt_end_i'), + sa.ForeignKeyConstraint(['exon_set_id'], ['uta.exon_set.exon_set_id'], onupdate='CASCADE', ondelete='CASCADE'), + sa.PrimaryKeyConstraint('exon_id'), + sa.UniqueConstraint('exon_set_id', 'end_i', name='end_i_must_be_unique_in_exon_set'), + sa.UniqueConstraint('exon_set_id', 'start_i', name='start_i_must_be_unique_in_exon_set'), + schema='uta' + ) + op.create_index(op.f('ix_uta_exon_exon_set_id'), 'exon', ['exon_set_id'], unique=False, schema='uta') + op.create_table('exon_aln', + sa.Column('exon_aln_id', sa.Integer(), autoincrement=True, nullable=False), + sa.Column('tx_exon_id', sa.Integer(), nullable=False), + sa.Column('alt_exon_id', sa.Integer(), nullable=False), + sa.Column('cigar', sa.Text(), nullable=False), + sa.Column('added', sa.DateTime(), nullable=False), + sa.Column('tx_aseq', sa.Text(), nullable=True), + sa.Column('alt_aseq', sa.Text(), nullable=True), + sa.ForeignKeyConstraint(['alt_exon_id'], ['uta.exon.exon_id'], onupdate='CASCADE', ondelete='CASCADE'), + sa.ForeignKeyConstraint(['tx_exon_id'], ['uta.exon.exon_id'], onupdate='CASCADE', ondelete='CASCADE'), + sa.PrimaryKeyConstraint('exon_aln_id'), + schema='uta' + ) + op.create_index(op.f('ix_uta_exon_aln_alt_exon_id'), 'exon_aln', ['alt_exon_id'], unique=False, schema='uta') + op.create_index(op.f('ix_uta_exon_aln_tx_exon_id'), 'exon_aln', ['tx_exon_id'], unique=False, schema='uta') + # ### end Alembic commands ### + + # ### custom commands to match the initial UTA database schema 1.1 ### + op.create_table('associated_accessions', + sa.Column('associated_accession_id', sa.Integer(), autoincrement=True, nullable=False), + sa.Column('tx_ac', sa.Text(), nullable=True), + sa.Column('pro_ac', sa.Text(), nullable=True), + sa.Column('origin', sa.Text(), nullable=False), + sa.Column('added', postgresql.TIMESTAMP(timezone=True), server_default=sa.text('now()'), nullable=False), + sa.PrimaryKeyConstraint('associated_accession_id'), + schema='uta' + ) + op.create_index('associated_accessions_tx_ac', 'associated_accessions', ['tx_ac'], unique=False, schema='uta') + op.create_index('associated_accessions_pro_ac', 'associated_accessions', ['pro_ac'], unique=False, schema='uta') + op.create_index('unique_pair_in_origin', 'associated_accessions', ['origin', 'tx_ac', 'pro_ac'], unique=True, schema='uta') + op.create_table_comment('associated_accessions', 'transcript-protein accession pairs associated in source databases', schema='uta') + # ### end custom commands ### + + # ### custom SQL to add views to match the initial UTA database schema 1.1 ### + op.execute(""" + CREATE VIEW _cds_exons_v AS + WITH cds_exons as ( + SELECT ES.exon_set_id, T.ac AS tx_ac, E.ord, + E.start_i, E.end_i, + CASE WHEN E.end_i >= T.cds_start_i AND E.start_i <= T.cds_end_i THEN greatest(E.start_i,T.cds_start_i) ELSE NULL end AS cds_ex_start_i, + CASE WHEN E.end_i >= T.cds_start_i AND E.start_i <= T.cds_end_i THEN least(E.end_i,T.cds_end_i) ELSE NULL end AS cds_ex_end_i + FROM transcript T + JOIN exon_set ES ON T.ac = ES.tx_ac AND ES.alt_aln_METHOD = 'transcript' + JOIN exon E ON ES.exon_set_id=E.exon_set_id + WHERE T.cds_start_i IS NOT NULL AND T.cds_end_i IS NOT NULL + ) + select *, end_i - start_i as ex_len, cds_ex_end_i - cds_ex_start_i as cds_ex_len from cds_exons; + """) + op.execute(""" + CREATE VIEW _cds_exons_flat_v AS + SELECT exon_set_id,tx_ac,MIN(ord) AS cds_start_exon,MAX(ord) AS cds_end_exon, + ARRAY_TO_STRING(ARRAY_AGG(format('%s,%s',cds_ex_start_i,cds_ex_end_i) ORDER BY ord),';') AS cds_se_i, + ARRAY_TO_STRING(ARRAY_AGG(cds_ex_len ORDER BY ord),';') AS cds_exon_lengths + FROM _cds_exons_v + WHERE cds_ex_start_i IS NOT NULL + GROUP BY exon_set_id, tx_ac; + """) + op.execute(""" + CREATE VIEW _seq_anno_most_recent AS + SELECT DISTINCT ON (ac) * + FROM seq_anno + ORDER BY ac,added DESC; + """) + op.execute(""" + CREATE VIEW _cds_exons_fp_v AS + SELECT SA.seq_id, md5(format('%s;%s',LOWER(SA.seq_id),CTEF.cds_se_i)) AS cds_es_fp, + md5(cds_exon_lengths) AS cds_exon_lengths_fp, CTEF.* + FROM _cds_exons_flat_v CTEF + JOIN _seq_anno_most_recent SA ON CTEF.tx_ac=SA.ac; + """) + op.execute(""" + CREATE VIEW _discontiguous_tx AS + SELECT t.hgnc, + es.exon_set_id, + es.tx_ac, + format('[%s-%s]'::text, e1.end_i, e2.start_i) AS gap, + e1.exon_id AS e1_exon_id, + e1.ord AS e1_ord, + e1.start_i AS e1_start_i, + e1.end_i AS e1_end_i, + e2.exon_id AS e2_exon_id, + e2.ord AS e2_ord, + e2.start_i AS e2_start_i, + e2.end_i AS e2_end_i + FROM exon_set es + LEFT JOIN transcript t ON es.tx_ac = t.ac + JOIN exon e1 ON es.exon_set_id = e1.exon_set_id + JOIN exon e2 ON es.exon_set_id = e2.exon_set_id AND e2.ord = (e1.ord + 1) AND e1.end_i <> e2.start_i + WHERE es.alt_aln_method = 'transcript'::text; + """) + op.execute(""" + CREATE VIEW tx_alt_exon_pairs_v AS + SELECT T.hgnc,TES.exon_SET_id AS tes_exon_SET_id,AES.exon_SET_id AS aes_exon_SET_id, + TES.tx_ac AS tx_ac,AES.alt_ac AS alt_ac,AES.alt_strand,AES.alt_aln_method, + TEX.ORD AS ORD,TEX.exon_id AS tx_exon_id,AEX.exon_id AS alt_exon_id, + TEX.start_i AS tx_start_i,TEX.END_i AS tx_END_i, AEX.start_i AS alt_start_i,AEX.END_i AS alt_END_i, + EA.exon_aln_id,EA.cigar + FROM exon_SET tes + JOIN transcript t ON tes.tx_ac=t.ac + JOIN exon_set aes ON tes.tx_ac=aes.tx_ac AND tes.alt_aln_method='transcript' AND aes.alt_aln_method!='transcript' + JOIN exon tex ON tes.exon_SET_id=tex.exon_SET_id + JOIN exon aex ON aes.exon_SET_id=aex.exon_SET_id AND tex.ORD=aex.ORD + LEFT JOIN exon_aln ea ON ea.tx_exon_id=tex.exon_id AND ea.alt_exon_id=AEX.exon_id; + """) + op.execute(""" + CREATE VIEW tx_exon_aln_v AS + SELECT T.hgnc,T.ac as tx_ac,AES.alt_ac,AES.alt_aln_method,AES.alt_strand, + TE.ord, TE.start_i as tx_start_i,TE.end_i as tx_end_i, + AE.start_i as alt_start_i, AE.end_i as alt_end_i, + EA.cigar, EA.tx_aseq, EA.alt_aseq, + TES.exon_set_id AS tx_exon_set_id,AES.exon_set_id as alt_exon_set_id, + TE.exon_id as tx_exon_id, AE.exon_id as alt_exon_id, + EA.exon_aln_id + FROM transcript T + JOIN exon_set TES ON T.ac=TES.tx_ac AND TES.alt_aln_method ='transcript' + JOIN exon_set AES on T.ac=AES.tx_ac and AES.alt_aln_method!='transcript' + JOIN exon TE ON TES.exon_set_id=TE.exon_set_id + JOIN exon AE ON AES.exon_set_id=AE.exon_set_id AND TE.ord=AE.ord + LEFT JOIN exon_aln EA ON TE.exon_id=EA.tx_exon_id AND AE.exon_id=EA.alt_exon_id; + """) + op.execute(""" + CREATE VIEW exon_set_exons_v AS + SELECT ES.*,EL.n_exons,EL.se_i,EL.starts_i,EL.ends_i,EL.lengths + FROM exon_set ES + JOIN (SELECT + iES.exon_set_id, + count(*) AS n_exons, + array_to_string(array_agg(format('%s,%s',iE.start_i,iE.end_i) ORDER BY iE.ord),';') AS se_i, + array_agg(iE.start_i ORDER BY iE.ord) AS starts_i, + array_agg(iE.end_i ORDER BY iE.ord) AS ends_i, + array_agg((iE.end_i-iE.start_i) ORDER BY iE.ord) AS lengths + FROM exon_set iES + JOIN exon iE ON iES.exon_set_id=iE.exon_set_id + GROUP BY iES.exon_set_id) EL + ON ES.exon_set_id = EL.exon_set_id; + """) + op.execute(""" + COMMENT ON VIEW exon_set_exons_v IS 'defining view of "flat" (aggregated) exons on a sequence; use _mv; for faster materialized version'; + """) + op.execute(""" + CREATE VIEW exon_set_exons_fp_v AS + SELECT ESE.*,md5(format('%s;%s',lower(ASA.seq_id),ESE.se_i)) AS es_fingerprint + FROM exon_set_exons_v ESE + JOIN _seq_anno_most_recent ASA ON ESE.alt_ac=ASA.ac; + """) + op.execute(""" + COMMENT ON VIEW exon_set_exons_fp_v IS 'flattened (aggregated) exons with exon set fingerprint'; + """) + op.execute(""" + CREATE MATERIALIZED VIEW exon_set_exons_fp_mv AS SELECT * FROM exon_set_exons_fp_v WITH NO DATA; + CREATE INDEX exon_set_exons_fp_mv_tx_ac_ix ON exon_set_exons_fp_mv(tx_ac); + CREATE INDEX exon_set_exons_fp_mv_alt_ac_ix ON exon_set_exons_fp_mv(alt_ac); + CREATE INDEX exon_set_exons_fp_mv_alt_aln_method_ix ON exon_set_exons_fp_mv(alt_aln_method); + GRANT SELECT ON exon_set_exons_fp_mv TO public; + """) + op.execute(""" + CREATE OR replace VIEW tx_exon_set_summary_dv AS + SELECT hgnc,cds_md5,es_fingerprint,tx_ac,alt_ac,alt_aln_method,alt_strand,exon_set_id,n_exons,se_i,starts_i,ends_i,lengths + FROM transcript T + JOIN exon_set_exons_fp_mv ESE ON T.ac=ESE.tx_ac; + """) + op.execute(""" + CREATE MATERIALIZED VIEW tx_exon_set_summary_mv AS SELECT * FROM tx_exon_set_summary_dv WITH NO DATA; + CREATE INDEX tx_exon_set_summary_mv_cds_md5_ix ON tx_exon_set_summary_mv(cds_md5); + CREATE INDEX tx_exon_set_summary_mv_es_fingerprint_ix ON tx_exon_set_summary_mv(es_fingerprint); + CREATE INDEX tx_exon_set_summary_mv_tx_ac_ix ON tx_exon_set_summary_mv(tx_ac); + CREATE INDEX tx_exon_set_summary_mv_alt_ac_ix ON tx_exon_set_summary_mv(alt_ac); + CREATE INDEX tx_exon_set_summary_mv_alt_aln_method_ix ON tx_exon_set_summary_mv(alt_aln_method); + GRANT SELECT ON tx_exon_set_summary_mv TO public; + """) + op.execute(""" + CREATE VIEW tx_def_summary_dv AS + SELECT TESS.exon_set_id, TESS.tx_ac, TESS.alt_ac, TESS.alt_aln_method, TESS.alt_strand, + TESS.hgnc, TESS.cds_md5, TESS.es_fingerprint, CEF.cds_es_fp, CEF.cds_exon_lengths_fp, + TESS.n_exons, TESS.se_i, CEF.cds_se_i, TESS.starts_i, TESS.ends_i, TESS.lengths, + T.cds_start_i, T.cds_end_i, CEF.cds_start_exon, CEF.cds_end_exon + FROM tx_exon_set_summary_mv TESS + JOIN transcript T ON TESS.tx_ac=T.ac + LEFT JOIN _cds_exons_fp_v CEF ON TESS.exon_set_id=CEF.exon_set_id + WHERE TESS.alt_aln_method = 'transcript'; + """) + op.execute(""" + COMMENT ON VIEW tx_def_summary_dv IS 'transcript definitions, with exon structures'; + """) + op.execute(""" + CREATE MATERIALIZED VIEW tx_def_summary_mv AS SELECT * FROM tx_def_summary_dv WITH NO DATA; + """) + op.execute(""" + COMMENT ON MATERIALIZED VIEW tx_def_summary_mv IS 'transcript definitions, with exon structures and fingerprints'; + """) + op.execute(""" + create index tx_def_summary_mv_tx_ac on tx_def_summary_mv (tx_ac); + create index tx_def_summary_mv_alt_ac on tx_def_summary_mv (alt_ac); + create index tx_def_summary_mv_alt_aln_method on tx_def_summary_mv (alt_aln_method); + create index tx_def_summary_mv_hgnc on tx_def_summary_mv (hgnc); + """) + op.execute(""" + CREATE VIEW tx_def_summary_v AS + SELECT * FROM tx_def_summary_mv; + """) + op.execute(""" + CREATE OR REPLACE VIEW tx_similarity_v AS + SELECT DISTINCT + D1.tx_ac as tx_ac1, D2.tx_ac as tx_ac2, + D1.hgnc = D2.hgnc as hgnc_eq, + D1.cds_md5=D2.cds_md5 as cds_eq, + D1.es_fingerprint=D2.es_fingerprint as es_fp_eq, + D1.cds_es_fp=D2.cds_es_fp as cds_es_fp_eq, + D1.cds_exon_lengths_fp=D2.cds_exon_lengths_fp as cds_exon_lengths_fp_eq + FROM tx_def_summary_mv D1 + JOIN tx_def_summary_mv D2 on (D1.tx_ac != D2.tx_ac + and (D1.hgnc=D2.hgnc + or D1.cds_md5=D2.cds_md5 + or D1.es_fingerprint=D2.es_fingerprint + or D1.cds_es_fp=D2.cds_es_fp + or D1.cds_exon_lengths_fp=D2.cds_exon_lengths_fp + )); + """) + # ### end custom SQL commands ### + + +def downgrade() -> None: + # ### custom SQL to remove views ### + op.execute("DROP VIEW tx_similarity_v CASCADE;") + op.execute("DROP VIEW tx_def_summary_v CASCADE;") + op.execute("DROP INDEX tx_def_summary_mv_hgnc CASCADE") + op.execute("DROP INDEX tx_def_summary_mv_alt_aln_method CASCADE") + op.execute("DROP INDEX tx_def_summary_mv_alt_ac CASCADE") + op.execute("DROP INDEX tx_def_summary_mv_tx_ac CASCADE") + op.execute("DROP MATERIALIZED VIEW tx_def_summary_mv CASCADE;") + op.execute("DROP VIEW tx_def_summary_dv CASCADE;") + op.execute("DROP MATERIALIZED VIEW tx_exon_set_summary_mv CASCADE;") + op.execute("DROP VIEW tx_exon_set_summary_dv CASCADE;") + op.execute("DROP MATERIALIZED VIEW exon_set_exons_fp_mv CASCADE;") + op.execute("DROP VIEW exon_set_exons_fp_v CASCADE;") + op.execute("DROP VIEW exon_set_exons_v CASCADE;") + op.execute("DROP VIEW tx_exon_aln_v CASCADE;") + op.execute("DROP VIEW tx_alt_exon_pairs_v CASCADE;") + op.execute("DROP VIEW _discontiguous_tx CASCADE;") + op.execute("DROP VIEW _cds_exons_fp_v CASCADE;") + op.execute("DROP VIEW _seq_anno_most_recent CASCADE;") + op.execute("DROP VIEW _cds_exons_flat_v CASCADE;") + op.execute("DROP VIEW _cds_exons_v CASCADE;") + # ### end custom SQL commands ### + + # ### commands auto generated by Alembic - please adjust! ### + op.drop_index(op.f('ix_uta_exon_aln_tx_exon_id'), table_name='exon_aln', schema='uta') + op.drop_index(op.f('ix_uta_exon_aln_alt_exon_id'), table_name='exon_aln', schema='uta') + op.drop_table('exon_aln', schema='uta') + op.drop_index(op.f('ix_uta_exon_exon_set_id'), table_name='exon', schema='uta') + op.drop_table('exon', schema='uta') + op.drop_table('exon_set', schema='uta') + op.drop_index(op.f('ix_uta_transcript_origin_id'), table_name='transcript', schema='uta') + op.drop_index(op.f('ix_uta_transcript_cds_md5'), table_name='transcript', schema='uta') + op.drop_table('transcript', schema='uta') + op.drop_index('seq_anno_ac_unique_in_origin', table_name='seq_anno', schema='uta') + op.drop_index(op.f('ix_uta_seq_anno_seq_id'), table_name='seq_anno', schema='uta') + op.drop_index(op.f('ix_uta_seq_anno_ac'), table_name='seq_anno', schema='uta') + op.drop_table('seq_anno', schema='uta') + op.drop_table('seq', schema='uta') + op.drop_table('origin', schema='uta') + op.drop_table('meta', schema='uta') + op.drop_table('gene', schema='uta') + # ### end Alembic commands ### + + # ### custom commands to remove items not autogenerated by Alembic ### + op.drop_index('unique_pair_in_origin', table_name='associated_accessions') + op.drop_index('associated_accessions_pro_ac', table_name='associated_accessions') + op.drop_index('associated_accessions_tx_ac', table_name='associated_accessions') + op.drop_table_comment('associated_accessions', existing_comment='transcript-protein accession pairs associated in source databases', schema='uta') + op.drop_table('associated_accessions') + # ### end custom commands ### \ No newline at end of file diff --git a/src/uta/models.py b/src/uta/models.py index 20c71b5..9a598b1 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -9,6 +9,7 @@ import sqlalchemy.types import sqlalchemy.sql.functions from sqlalchemy.ext.declarative import declarative_base +from sqlalchemy.dialects import postgresql ############################################################################ @@ -18,11 +19,9 @@ schema_version = "1.1" use_schema = True if use_schema: - schema_name = "uta_" + schema_version.replace(".","_") - schema_name_dot = schema_name + "." + schema_name = "uta" else: schema_name = None - schema_name_dot = "" ############################################################################ @@ -210,8 +209,8 @@ class ExonAln(Base): cigar = sa.Column(sa.Text, nullable=False) added = sa.Column( sa.DateTime, default=datetime.datetime.now(), nullable=False) - tx_aseq = sa.Column(sa.Text, nullable=False) - alt_aseq = sa.Column(sa.Text, nullable=False) + tx_aseq = sa.Column(sa.Text, nullable=True) + alt_aseq = sa.Column(sa.Text, nullable=True) # relationships: tx_exon = sao.relationship( @@ -225,9 +224,10 @@ class ExonAln(Base): class AssociatedAccessions(Base): __tablename__ = "associated_accessions" __table_args__ = ( - sa.UniqueConstraint("origin", "tx_ac", "pro_ac", name="unique_pair_in_origin"), + sa.Index("unique_pair_in_origin", "origin", "tx_ac", "pro_ac", unique=True), sa.Index("associated_accessions_pro_ac", "pro_ac"), sa.Index("associated_accessions_tx_ac", "tx_ac"), + {"comment": "transcript-protein accession pairs associated in source databases"}, ) # columns: @@ -236,7 +236,7 @@ class AssociatedAccessions(Base): pro_ac = sa.Column(sa.Text, nullable=False) origin = sa.Column(sa.Text, nullable=False) added = sa.Column( - sqlalchemy.types.TIMESTAMP, + postgresql.TIMESTAMP(timezone=True), server_default=sqlalchemy.sql.functions.now(), nullable=False, ) From 49e08e9b510d2b6fe1dc3fff6f54780e47cf528c Mon Sep 17 00:00:00 2001 From: Paige Date: Fri, 5 Apr 2024 12:07:06 -0700 Subject: [PATCH 29/54] feat(IPVC-2276): skip accessions from gff that are not in the tx_info file, log missing acs to file (#18) --- etc/scripts/run-uta-build.sh | 8 +++- sbin/filter_exonset_transcripts.py | 51 ++++++++++++++++++++++++ sbin/ncbi_parse_genomic_gff.py | 17 ++------ src/uta/tools/file_utils.py | 12 ++++++ tests/test_filter_exonset_transcripts.py | 46 +++++++++++++++++++++ tests/test_ncbi_parse_genomic_gff.py | 6 +-- 6 files changed, 121 insertions(+), 19 deletions(-) create mode 100755 sbin/filter_exonset_transcripts.py create mode 100644 src/uta/tools/file_utils.py create mode 100644 tests/test_filter_exonset_transcripts.py diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh index f4dec9a..613d6ce 100755 --- a/etc/scripts/run-uta-build.sh +++ b/etc/scripts/run-uta-build.sh @@ -64,8 +64,12 @@ GFF_files=$(ls $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_as sbin/ncbi_parse_genomic_gff.py "$GFF_files" | gzip -c > "$loading_dir/gff.exonsets.gz" 2>&1 | \ tee "$logs_dir/ncbi-parse-genomic-gff.log" +sbin/filter_exonset_transcripts.py --tx-info "$loading_dir/gbff.txinfo.gz" --exonsets "$loading_dir/gff.exonsets.gz" \ + --missing-ids "$loading_dir/filtered_tx_acs.txt" | gzip -c > "$loading_dir/gff.filtered_exonsets.gz" 2>&1 | \ + tee "$logs_dir/filter_exonset_transcripts.log" + # generate seqinfo files from exonsets -sbin/exonset-to-seqinfo -o NCBI "$loading_dir/gff.exonsets.gz" | gzip -c > "$loading_dir/seqinfo.gz" 2>&1 | \ +sbin/exonset-to-seqinfo -o NCBI "$loading_dir/gff.filtered_exonsets.gz" | gzip -c > "$loading_dir/seqinfo.gz" 2>&1 | \ tee "$logs_dir/exonset-to-seqinfo.log" ### update the uta database @@ -81,7 +85,7 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$loadi tee "$logs_dir/load-txinfo.log" # gff exon sets -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$loading_dir/gff.exonsets.gz" 2>&1 | \ +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$loading_dir/gff.filtered_exonsets.gz" 2>&1 | \ tee "$logs_dir/load-exonsets.log" # align exons diff --git a/sbin/filter_exonset_transcripts.py b/sbin/filter_exonset_transcripts.py new file mode 100755 index 0000000..b3d1fc2 --- /dev/null +++ b/sbin/filter_exonset_transcripts.py @@ -0,0 +1,51 @@ +#!/usr/bin/env python + +import argparse +import csv +import logging.config +import sys + +import importlib_resources + +from uta.formats.exonset import ExonSetReader, ExonSetWriter +from uta.formats.txinfo import TxInfoReader +from uta.tools.file_utils import open_file + +logging_conf_fn = importlib_resources.files("uta").joinpath("etc/logging.conf") +logging.config.fileConfig(logging_conf_fn) +logging.getLogger().setLevel(logging.INFO) +logger = logging.getLogger(__name__) + + +def filter_exonset(exonset_file, transcript_ids, missing_ids_file): + with open_file(exonset_file) as es_f, open(missing_ids_file, 'w') as missing_f: + exonsets = ExonSetReader(es_f) + esw = ExonSetWriter(sys.stdout) + writer_missing = csv.writer(missing_f) + missing_acs = set() + + for exonset in exonsets: + if exonset.tx_ac in transcript_ids: + esw.write(exonset) + else: + logger.warning(f"Exon set transcript {exonset.tx_ac} not found in txinfo file. Filtering out.") + writer_missing.writerow([exonset.tx_ac]) + missing_acs.add(exonset.tx_ac) + logger.info(f"Filtered out exon sets for {len(missing_acs)} transcript(s): {','.join(missing_acs)}") + + +def main(): + parser = argparse.ArgumentParser(description='Filter exonset data.') + parser.add_argument('--tx-info', help='Path to the transcript info file') + parser.add_argument('--exonsets', help='Path to the exonset file') + parser.add_argument('--missing-ids', help='Path to the missing transcript ids file') + args = parser.parse_args() + + with open_file(args.tx_info) as f: + tx_reader = TxInfoReader(f) + transcript_ids = {row.ac for row in tx_reader} + filter_exonset(args.exonsets, transcript_ids, args.missing_ids) + + +if __name__ == '__main__': + main() diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 8fb1169..728e8a5 100755 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -24,27 +24,16 @@ """ import argparse -import gzip import logging.config import sys from collections import defaultdict -from contextlib import contextmanager from dataclasses import dataclass from typing import List, Optional import pkg_resources from uta.formats.exonset import ExonSet, ExonSetWriter - - -@contextmanager -def open_file(filename): - if filename.endswith(".gz"): - with gzip.open(filename, "rt") as f: - yield f - else: - with open(filename) as f: - yield f +from uta.tools.file_utils import open_file @dataclass @@ -115,7 +104,7 @@ def _get_exon_number_from_id(alignment_id: str) -> int: return int(alignment_id.split("-")[-1]) -def parse_gff_file(file_paths: List[str]) -> dict[str, List[GFFRecord]]: +def parse_gff_files(file_paths: List[str]) -> dict[str, List[GFFRecord]]: tx_data = defaultdict(list) for file_path in file_paths: with open_file(file_path) as f: @@ -152,7 +141,7 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: gff_files = args.gff_files esw = ExonSetWriter(sys.stdout) - transcript_alignments = parse_gff_file(gff_files) + transcript_alignments = parse_gff_files(gff_files) logger.info( f"read {len(transcript_alignments)} transcript alignments from file(s): {', '.join(gff_files)}" ) diff --git a/src/uta/tools/file_utils.py b/src/uta/tools/file_utils.py new file mode 100644 index 0000000..78d8d3d --- /dev/null +++ b/src/uta/tools/file_utils.py @@ -0,0 +1,12 @@ +import gzip +from contextlib import contextmanager + + +@contextmanager +def open_file(filename): + if filename.endswith(".gz"): + with gzip.open(filename, "rt") as f: + yield f + else: + with open(filename) as f: + yield f diff --git a/tests/test_filter_exonset_transcripts.py b/tests/test_filter_exonset_transcripts.py new file mode 100644 index 0000000..7ae9cf6 --- /dev/null +++ b/tests/test_filter_exonset_transcripts.py @@ -0,0 +1,46 @@ +import contextlib +import io +import unittest +from tempfile import NamedTemporaryFile +from unittest.mock import patch + +from sbin.filter_exonset_transcripts import filter_exonset + + +class TestFilterExonsetTranscripts(unittest.TestCase): + + @patch('sbin.filter_exonset_transcripts.logger') + def test_filter_exonset(self, mock_logger): + # Test NR_046571.1 is filtered out + lines = [ + "tx_ac\talt_ac\tmethod\tstrand\texons_se_i\n", + "NR_122113.1\tNC_000022.10\tsplign\t-1\t16192905,16193009;16190680,16190791;16189263,16189378;16189031,16189143;16187164,16187302;16186810,16186953;16162396,16162487;16150528,16151821\n", + "NR_133911.1\tNC_000022.10\tsplign\t1\t16157078,16157342;16164481,16164569;16171951,16172265\n", + "NR_046571.1\tNC_000022.10\tsplign\t1\t16274608,16275003;16276480,16277577\n" + ] + with NamedTemporaryFile(delete=False) as temp_exonsets: + with open(temp_exonsets.name, "wt") as f: + for line in lines: + f.write(line) + temp_exonsets.seek(0) + missing_ids_file = NamedTemporaryFile() + + transcript_ids = {"NR_122113.1", "NR_133911.1"} + stdout = io.StringIO() + with contextlib.redirect_stdout(stdout): + filter_exonset(temp_exonsets.name, transcript_ids, missing_ids_file.name) + + # Assert the record for NR_046571.1 is filtered out + self.assertEqual(stdout.getvalue(), ''.join(lines[0:3])) + + # Confirm filtered transcript is present in missing_ids_file + with open(missing_ids_file.name, 'r') as f: + contents = f.read() + self.assertEqual(contents, 'NR_046571.1\n') + + mock_logger.warning.assert_called_with('Exon set transcript NR_046571.1 not found in txinfo file. Filtering out.') + mock_logger.info.assert_called_with('Filtered out exon sets for 1 transcript(s): NR_046571.1') + + +if __name__ == "__main__": + unittest.main() diff --git a/tests/test_ncbi_parse_genomic_gff.py b/tests/test_ncbi_parse_genomic_gff.py index 71d92f9..b40edeb 100644 --- a/tests/test_ncbi_parse_genomic_gff.py +++ b/tests/test_ncbi_parse_genomic_gff.py @@ -7,7 +7,7 @@ from sbin.ncbi_parse_genomic_gff import ( get_zero_based_exon_ranges, GFFRecord, - parse_gff_file, + parse_gff_files, parse_gff_record, ) @@ -160,7 +160,7 @@ def test_parse_gff_record_raises_unparseable_id(self): def test_parse_gff_file(self): # Test parsing the entire uncompressed GFF file expected_result = {"rna-NR_046018.2": self.gff_records} - parsed_result = parse_gff_file([self.temp_gff.name]) + parsed_result = parse_gff_files([self.temp_gff.name]) self.assertEqual(parsed_result, expected_result) def test_parse_gff_file_accepts_gzipped_files(self): @@ -171,7 +171,7 @@ def test_parse_gff_file_accepts_gzipped_files(self): # Test parsing the gzipped GFF file expected_result = {"rna-NR_046018.2": self.gff_records} - parsed_result = parse_gff_file([self.temp_gff.name + ".gz"]) + parsed_result = parse_gff_files([self.temp_gff.name + ".gz"]) self.assertEqual(parsed_result, expected_result) def test_get_zero_based_exon_ranges(self): From a072cb15596b7ae433b03fed773c19a734735e90 Mon Sep 17 00:00:00 2001 From: Paige Date: Mon, 8 Apr 2024 14:07:17 -0700 Subject: [PATCH 30/54] feat(IPVC-2280): add codon_table to transcript + migration (#21) --- README.md | 15 ++++++++++ ...7b584f699_add_codon_table_to_transcript.py | 30 +++++++++++++++++++ src/uta/models.py | 1 + 3 files changed, 46 insertions(+) create mode 100644 src/alembic/versions/a697b584f699_add_codon_table_to_transcript.py diff --git a/README.md b/README.md index 78da019..84e606c 100644 --- a/README.md +++ b/README.md @@ -342,3 +342,18 @@ Example: ``` sbin/uta-update $(pwd)/ncbi-data $(pwd)/seqrepo-data $(pwd)/uta-build uta_20210129b 2024-02-20 ``` + +## Migrations +UTA uses alembic to manage database migrations. To auto-generate a migration: +``` +alembic -c etc/alembic.ini revision --autogenerate -m "description of the migration" +``` +This will create a migration script in the alembic/versions directory. +Adjust the upgrade and downgrade function definitions. To apply the migration: +``` +alembic -c etc/alembic.ini upgrade head +``` +To reverse a migration, use `downgrade` with the number of steps to reverse. For example, to reverse the last: +``` +alembic -c etc/alembic.ini downgrade -1 +``` diff --git a/src/alembic/versions/a697b584f699_add_codon_table_to_transcript.py b/src/alembic/versions/a697b584f699_add_codon_table_to_transcript.py new file mode 100644 index 0000000..cb6eec1 --- /dev/null +++ b/src/alembic/versions/a697b584f699_add_codon_table_to_transcript.py @@ -0,0 +1,30 @@ +"""add codon_table to Transcript + +Revision ID: a697b584f699 +Revises: cc51f50ae896 +Create Date: 2024-04-08 17:27:41.570024 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa + + +# revision identifiers, used by Alembic. +revision: str = 'a697b584f699' +down_revision: Union[str, None] = 'cc51f50ae896' +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.add_column('transcript', sa.Column('codon_table', sa.Integer(), server_default='1', nullable=False), schema='uta') + # ### end Alembic commands ### + + +def downgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.drop_column('transcript', 'codon_table', schema='uta') + # ### end Alembic commands ### diff --git a/src/uta/models.py b/src/uta/models.py index 9a598b1..31a5392 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -130,6 +130,7 @@ class Transcript(Base): cds_md5 = sa.Column(sa.Text, index=True) added = sa.Column( sa.DateTime, default=datetime.datetime.now(), nullable=False) + codon_table = sa.Column(sa.Integer, nullable=False, server_default='1') # 1 = standard, 2 = mitochondrial # relationships: origin = sao.relationship("Origin", backref="transcripts") From d65d764c3e54f974511f95b9dfe55ecfae7c3bb4 Mon Sep 17 00:00:00 2001 From: nvta1209 <162694616+nvta1209@users.noreply.github.com> Date: Tue, 9 Apr 2024 08:07:55 -0700 Subject: [PATCH 31/54] feat(IPVC-2283): mitochondrial transcript workflow (#20) --- README.md | 95 +++++++++++++++++++------------- docker-compose.yml | 50 ++++++++++++++--- etc/scripts/run-uta-build.sh | 98 --------------------------------- sbin/ncbi-download | 24 ++++++++ sbin/ncbi-download-docker | 24 -------- sbin/ncbi_process_mito.py | 23 ++++---- sbin/seqrepo-download | 53 ------------------ sbin/seqrepo-load | 21 +++++++ sbin/uta-extract | 43 +++++++++++++++ sbin/uta-load | 77 ++++++++++++++++++++++++++ sbin/uta-update | 67 ---------------------- tests/test_ncbi_process_mito.py | 8 +-- 12 files changed, 280 insertions(+), 303 deletions(-) delete mode 100755 etc/scripts/run-uta-build.sh delete mode 100755 sbin/ncbi-download-docker mode change 100644 => 100755 sbin/ncbi_process_mito.py delete mode 100755 sbin/seqrepo-download create mode 100755 sbin/seqrepo-load create mode 100755 sbin/uta-extract create mode 100755 sbin/uta-load delete mode 100755 sbin/uta-update diff --git a/README.md b/README.md index 84e606c..2ade8c5 100644 --- a/README.md +++ b/README.md @@ -289,60 +289,77 @@ To develop UTA, follow these steps. 4. Testing $ docker build --target uta-test -t uta-test . - $ docker run -it --rm uta-test python -m unittest + $ docker run --rm uta-test python -m unittest ## UTA update procedure -### 1. Download files from NCBI +Requires docker. -Run `sbin/ncbi-download-docker`. Requires bash and docker. +### 0. Setup -Example: +Make directories: ``` -sbin/ncbi-download-docker $(pwd)/ncbi-data +mkdir -p $(pwd)/ncbi-data +mkdir -p $(pwd)/output/artifacts +mkdir -p $(pwd)/output/logs ``` -The specified directory will have the following structure: - - ├── gene - │ └── DATA - │ ├── GENE_INFO - │ │ └── Mammalia - │ │ └── Homo_sapiens.gene_info.gz - │ └── gene2accession.gz - ├── genomes - │ └── refseq - │ └── vertebrate_mammalian - │ └── Homo_sapiens - │ └── all_assembly_versions - │ └── GCF_000001405.25_GRCh37.p13 - │ ├── GCF_000001405.25_GRCh37.p13_genomic.fna.gz - │ └── GCF_000001405.25_GRCh37.p13_genomic.gff.gz - └── refseq - └── H_sapiens - └── mRNA_Prot - ├── human.1.protein.faa.gz - ├── human.1.rna.fna.gz - └── human.1.rna.gbff.gz - -### 2. Download SeqRepo data - -Run `sbin/seqrepo-download`. Requires bash and docker. - -Example: +Set variables: ``` -sbin/seqrepo-download 2024-02-20 $(pwd)/seqrepo-data +export UTA_ETL_OLD_SEQREPO_VERSION=2024-02-20 +export UTA_ETL_OLD_UTA_VERSION=uta_20210129b +export UTA_ETL_NCBI_DIR=./ncbi-data +export UTA_ETL_SEQREPO_DIR=./seqrepo-data +export UTA_ETL_WORK_DIR=./output/artifacts +export UTA_ETL_LOG_DIR=./output/logs ``` -### 3. Update UTA and SeqRepo +Build the UTA image: +``` +docker build --target uta -t uta-update . +``` + +### 1. Download SeqRepo data +``` +docker pull biocommons/seqrepo:$UTA_ETL_OLD_SEQREPO_VERSION + +# download seqrepo. can skip if container already exists. +docker run --name seqrepo biocommons/seqrepo:$UTA_ETL_OLD_SEQREPO_VERSION + +# copy seqrepo data into a local directory +docker run -v $UTA_ETL_SEQREPO_DIR:/output-dir --volumes-from seqrepo ubuntu bash -c 'cp -R /usr/local/share/seqrepo/* /output-dir' + +# allow seqrepo to be modified +docker run -it -v $UTA_ETL_SEQREPO_DIR:/output-dir ubuntu bash -c 'chmod -R +w /output-dir' +``` -Run `sbin/uta-update`. Requires bash and docker. +Note: pulling data takes ~30 minutes and requires ~13 GB. +Note: a container called seqrepo will be left behind. -Example: +### 2. Extract and transform data from NCBI + +Download files from NCBI, extract into intermediate files, and load into UTA and SeqRepo. + +See 2A for nuclear transcripts and 2B for mitochondrial transcripts. + +#### 2A. Nuclear transcripts +``` +docker compose run ncbi-download +docker compose run uta-extract +docker compose run seqrepo-load +UTA_ETL_SKIP_GENE_LOAD=false docker compose run uta-load ``` -sbin/uta-update $(pwd)/ncbi-data $(pwd)/seqrepo-data $(pwd)/uta-build uta_20210129b 2024-02-20 + +#### 2B. Mitochondrial transcripts +``` +docker compose run mito-extract +docker compose run seqrepo-load +UTA_ETL_SKIP_GENE_LOAD=true docker compose run uta-load ``` +UTA has updated and the database has been dumped into a pgd file in `UTA_ETL_WORK_DIR`. SeqRepo has been updated in place. + + ## Migrations UTA uses alembic to manage database migrations. To auto-generate a migration: ``` @@ -353,7 +370,7 @@ Adjust the upgrade and downgrade function definitions. To apply the migration: ``` alembic -c etc/alembic.ini upgrade head ``` -To reverse a migration, use `downgrade` with the number of steps to reverse. For example, to reverse the last: +To reverse a migration, use `downgrade` with the number of steps to reverse. For example, to reverse the last: ``` alembic -c etc/alembic.ini downgrade -1 ``` diff --git a/docker-compose.yml b/docker-compose.yml index 856a5ae..1247c09 100644 --- a/docker-compose.yml +++ b/docker-compose.yml @@ -3,24 +3,60 @@ version: '3' services: + ncbi-download: + image: uta-update + command: sbin/ncbi-download /ncbi-dir + volumes: + - .:/opt/repos/uta + - ${UTA_ETL_NCBI_DIR}:/ncbi-dir + working_dir: /opt/repos/uta + network_mode: host + uta-extract: + image: uta-update + command: sbin/uta-extract /ncbi-dir /uta-extract/work /uta-extract/logs + volumes: + - ${UTA_ETL_NCBI_DIR}:/ncbi-dir + - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo + - ${UTA_ETL_WORK_DIR}:/uta-extract/work + - ${UTA_ETL_LOG_DIR}:/uta-extract/logs + working_dir: /opt/repos/uta + network_mode: host + seqrepo-load: + image: uta-update + command: sbin/seqrepo-load /usr/local/share/seqrepo 2024-02-20 /seqrepo-load/work /seqrepo-load/logs + volumes: + - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo + - ${UTA_ETL_WORK_DIR}:/seqrepo-load/work + - ${UTA_ETL_LOG_DIR}:/seqrepo-load/logs + working_dir: /opt/repos/uta + network_mode: host uta: container_name: uta - image: biocommons/uta:${UTA_VERSION} + image: biocommons/uta:${UTA_ETL_OLD_UTA_VERSION} environment: - POSTGRES_HOST_AUTH_METHOD=trust healthcheck: - test: psql -h localhost -U anonymous -d uta -c "select * from ${UTA_VERSION}.meta" + test: psql -h localhost -U anonymous -d uta -c "select * from ${UTA_ETL_OLD_UTA_VERSION}.meta" interval: 10s retries: 60 network_mode: host - uta-update: + uta-load: image: uta-update - command: etc/scripts/run-uta-build.sh ${UTA_VERSION} ${SEQREPO_VERSION} /ncbi-dir /workdir + command: sbin/uta-load ${UTA_ETL_OLD_UTA_VERSION} /ncbi-dir /uta-load/work /uta-load/logs ${UTA_ETL_SKIP_GENE_LOAD} depends_on: uta: condition: service_healthy volumes: - - ${NCBI_DIR}:/ncbi-dir - - ${SEQREPO_DIR}:/usr/local/share/seqrepo - - ${WORKING_DIR}:/workdir + - ${UTA_ETL_NCBI_DIR}:/ncbi-dir + - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo + - ${UTA_ETL_WORK_DIR}:/uta-load/work + - ${UTA_ETL_LOG_DIR}:/uta-load/logs + network_mode: host + mito-extract: + image: uta-update + command: sbin/ncbi_process_mito.py NC_012920.1 --output-dir /mito-extract/work | tee /mito-extract/logs/mito.log + volumes: + - ${UTA_ETL_WORK_DIR}:/mito-extract/work + - ${UTA_ETL_LOG_DIR}:/mito-extract/logs + working_dir: /opt/repos/uta network_mode: host diff --git a/etc/scripts/run-uta-build.sh b/etc/scripts/run-uta-build.sh deleted file mode 100755 index 613d6ce..0000000 --- a/etc/scripts/run-uta-build.sh +++ /dev/null @@ -1,98 +0,0 @@ -#!/bin/bash - -# source_uta_v is the UTA version before the update. -# seqrepo_data_release is the SeqRepo version before the update. -# ncbi_dir is where the script looks for NCBI data files. -# working_dir stores log files, intermediate data files, and the final database dump. - -set -euxo pipefail - -source_uta_v=$1 -seqrepo_data_release=$2 -ncbi_dir=$3 -working_dir=$4 - -if [ -z "$source_uta_v" ] || [ -z "$seqrepo_data_release" ] || [ -z "$ncbi_dir" ] || [ -z "$working_dir" ] -then - echo 'Usage: run-uta-build.sh ' - exit 1 -fi - -# set local variables and create working directories -loading_uta_v="uta" -loading_dir="$working_dir/loading" -dumps_dir="$working_dir/dumps" -logs_dir="$working_dir/logs" -for d in "$loading_dir" "$dumps_dir" "$logs_dir"; - do mkdir -p "$d" -done - -## Drop loading schema, and recreate -etc/scripts/delete-schema.sh "$loading_uta_v" -etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_uta_v" - -## for now set up Alembic for schema migrations -alembic -c etc/alembic.ini stamp edadb97f6502 -alembic -c etc/alembic.ini upgrade head - -## Load SeqRepo with new sequences -seqrepo load -n NCBI -i "$seqrepo_data_release" \ - $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz \ - $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz \ - $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.fna.gz 2>& 1 | \ - tee "$logs_dir/seqrepo-load.log" - -### extract meta data -# genes -sbin/ncbi-parse-geneinfo $ncbi_dir/gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz | \ - gzip -c > "$loading_dir/genes.geneinfo.gz" 2>&1 | tee "$logs_dir/ncbi-parse-geneinfo.log" - -# transcript protein associations -sbin/ncbi-parse-gene2refseq $ncbi_dir/gene/DATA/gene2accession.gz | gzip -c > "$loading_dir/assocacs.gz" 2>&1 | \ - tee "$logs_dir/ncbi-fetch-assoc-acs" - -sbin/assoc-acs-merge "$loading_dir/assocacs.gz" | gzip -c > "$loading_dir/assocacs.cleaned.gz" 2>&1 | \ - tee "$logs_dir/assoc-acs-merge" - -# parse transcript info from GBFF input files -GBFF_files=$(ls $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz) -sbin/ncbi-parse-gbff "$GBFF_files" | gzip -c > "$loading_dir/gbff.txinfo.gz" 2>&1 | \ - tee "$logs_dir/ncbi-parse-gbff.log" - -# parse alignments from GFF input files -GFF_files=$(ls $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.gff.gz) -sbin/ncbi_parse_genomic_gff.py "$GFF_files" | gzip -c > "$loading_dir/gff.exonsets.gz" 2>&1 | \ - tee "$logs_dir/ncbi-parse-genomic-gff.log" - -sbin/filter_exonset_transcripts.py --tx-info "$loading_dir/gbff.txinfo.gz" --exonsets "$loading_dir/gff.exonsets.gz" \ - --missing-ids "$loading_dir/filtered_tx_acs.txt" | gzip -c > "$loading_dir/gff.filtered_exonsets.gz" 2>&1 | \ - tee "$logs_dir/filter_exonset_transcripts.log" - -# generate seqinfo files from exonsets -sbin/exonset-to-seqinfo -o NCBI "$loading_dir/gff.filtered_exonsets.gz" | gzip -c > "$loading_dir/seqinfo.gz" 2>&1 | \ - tee "$logs_dir/exonset-to-seqinfo.log" - -### update the uta database -# genes -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$loading_dir/genes.geneinfo.gz" 2>&1 | \ - tee "$logs_dir/load-geneinfo.log" - -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-assoc-ac "$loading_dir/assocacs.cleaned.gz" 2>&1 | \ - tee "$logs_dir/load-assoc-ac.log" - -# transcript info -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$loading_dir/gbff.txinfo.gz" 2>&1 | \ - tee "$logs_dir/load-txinfo.log" - -# gff exon sets -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$loading_dir/gff.filtered_exonsets.gz" 2>&1 | \ - tee "$logs_dir/load-exonsets.log" - -# align exons -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | tee "$logs_dir/align-exons.log" - -### run diff -sbin/uta-diff "$source_uta_v" "$loading_uta_v" - -### psql_dump -pg_dump -U uta_admin -h localhost -d uta -t "$loading_uta_v.gene" | gzip -c > "$dumps_dir/uta.pgd.gz" diff --git a/sbin/ncbi-download b/sbin/ncbi-download index 61a6874..dc445cd 100755 --- a/sbin/ncbi-download +++ b/sbin/ncbi-download @@ -1,6 +1,29 @@ #!/usr/bin/env bash # This script downloads the files needed for a UTA+SeqRepo update into to the given directory. +# +# DONWLOAD_DIR will have the following structure: +# +# ├── gene +# │ └── DATA +# │ ├── GENE_INFO +# │ │ └── Mammalia +# │ │ └── Homo_sapiens.gene_info.gz +# │ └── gene2accession.gz +# ├── genomes +# │ └── refseq +# │ └── vertebrate_mammalian +# │ └── Homo_sapiens +# │ └── all_assembly_versions +# │ └── GCF_000001405.25_GRCh37.p13 +# │ ├── GCF_000001405.25_GRCh37.p13_genomic.fna.gz +# │ └── GCF_000001405.25_GRCh37.p13_genomic.gff.gz +# └── refseq +# └── H_sapiens +# └── mRNA_Prot +# ├── human.1.protein.faa.gz +# ├── human.1.rna.fna.gz +# └── human.1.rna.gbff.gz set -e @@ -26,6 +49,7 @@ do DOWNLOAD_MODULE="${DOWNLOAD_PATH%%/*}" DOWNLOAD_SRC="ftp.ncbi.nlm.nih.gov::$DOWNLOAD_PATH" DOWNLOAD_DST="$DOWNLOAD_DIR/$DOWNLOAD_MODULE" + mkdir -p $DOWNLOAD_DST echo "Downloading $DOWNLOAD_SRC to $DOWNLOAD_DST" rsync --no-motd -DHPRprtv "$DOWNLOAD_SRC" "$DOWNLOAD_DST" done diff --git a/sbin/ncbi-download-docker b/sbin/ncbi-download-docker deleted file mode 100755 index 30d4f63..0000000 --- a/sbin/ncbi-download-docker +++ /dev/null @@ -1,24 +0,0 @@ -#!/usr/bin/env bash - -# This script runs ncbi-download in a docker container. - -set -e - -DOWNLOAD_DIR=$1 - -if [ -z "$DOWNLOAD_DIR" ] -then - echo 'Usage: sbin/ncbi-download-docker ' - exit 1 -else - echo "Downloading files to $DOWNLOAD_DIR" -fi - -if [[ $DOWNLOAD_DIR != /* ]] -then - echo 'Download directory must be an absolute path' - exit 1 -fi - -docker build --target uta -t ncbi-download --progress plain . -docker run -it -v $(pwd)/sbin/ncbi-download:/dl-script -v $DOWNLOAD_DIR:/output-dir ncbi-download /dl-script /output-dir diff --git a/sbin/ncbi_process_mito.py b/sbin/ncbi_process_mito.py old mode 100644 new mode 100755 index 2afe071..c65984b --- a/sbin/ncbi_process_mito.py +++ b/sbin/ncbi_process_mito.py @@ -1,3 +1,5 @@ +#!/usr/bin/env python + """ Download mito fasta and gbff file. Use BioPython to parse the features in the Mitochondrial genbank file to get the attributes of a region of the genome that correspond to genes along with their attributes. Output gene/tx/alignment @@ -52,6 +54,7 @@ """ import argparse import dataclasses +import gzip import importlib_resources import logging import logging.config @@ -83,7 +86,7 @@ class MitoGeneData: alt_ac: str alt_start: int alt_end: int - strand: str + strand: int origin: str = "NCBI" alignment_method: str = "splign" transl_table: Optional[str] = None @@ -166,6 +169,7 @@ def parse_nomenclature_value(gb_feature: SeqFeature) -> Dict[str, str]: def get_mito_genes(gbff_filepath: str): logger.info(f"processing NCBI GBFF file from {gbff_filepath}") with open(gbff_filepath) as fh: + # Bio.SeqIO.parse(fh, "gb") returns an empty iterator for .fna files and does not fail for record in Bio.SeqIO.parse(fh, "gb"): for feature in record.features: xrefs = parse_db_xrefs(feature) @@ -200,9 +204,7 @@ def get_mito_genes(gbff_filepath: str): # retrieve sequence, and reverse compliment if on reverse strand ac = f"{record.id}_{feature.location.start:05}_{feature.location.end:05}" feature_seq = record.seq[feature_start:feature_end] - strand = "+" if feature.location.strand == -1: - strand = "-" feature_seq = feature_seq.reverse_complement() if feature.type == "CDS": @@ -225,7 +227,7 @@ def get_mito_genes(gbff_filepath: str): alt_ac=record.id, alt_start=feature_start, alt_end=feature_end, - strand=strand, + strand=feature.location.strand, transl_table=transl_table, transl_except=transl_except, pro_ac=pro_ac, @@ -242,7 +244,7 @@ def main(ncbi_accession: str, output_dir: str): logger.info(f"found {len(mito_genes)} genes from parsing {input_files['gbff']}") # write gene accessions - with open(f"{output_dir}/{ncbi_accession}.assocacs", "w") as o_file: + with gzip.open(f"{output_dir}/assocacs.gz", "wt") as o_file: gaw = GeneAccessionsWriter(o_file) for mg in mito_genes: if mg.pro_ac is not None: @@ -253,7 +255,7 @@ def main(ncbi_accession: str, output_dir: str): ) # write sequence information - with open(f"{output_dir}/{ncbi_accession}.seqinfo", "w") as o_file: + with gzip.open(f"{output_dir}/seqinfo.gz", "wt") as o_file: siw = SeqInfoWriter(o_file) for mg in mito_genes: siw.write( @@ -279,7 +281,7 @@ def main(ncbi_accession: str, output_dir: str): ) # write out transcript sequence fasta files. - with open(f"{output_dir}/{ncbi_accession}.rna.fna", "w") as o_file: + with gzip.open(f"{output_dir}/{ncbi_accession}.rna.fna.gz", "wt") as o_file: for mg in mito_genes: record = SeqRecord( Seq(mg.tx_seq), @@ -289,7 +291,7 @@ def main(ncbi_accession: str, output_dir: str): o_file.write(record.format("fasta")) # write out protein sequence fasta files. - with open(f"{output_dir}/{ncbi_accession}.protein.faa", "w") as o_file: + with gzip.open(f"{output_dir}/{ncbi_accession}.protein.faa.gz", "wt") as o_file: for mg in mito_genes: if mg.pro_ac is not None: record = SeqRecord( @@ -300,7 +302,7 @@ def main(ncbi_accession: str, output_dir: str): o_file.write(record.format("fasta")) # write transcript information - with open(f"{output_dir}/{ncbi_accession}.txinfo", "w") as o_file: + with gzip.open(f"{output_dir}/txinfo.gz", "wt") as o_file: tiw = TxInfoWriter(o_file) for mg in mito_genes: tiw.write( @@ -315,7 +317,7 @@ def main(ncbi_accession: str, output_dir: str): ) # write exonset - with open(f"{output_dir}/{ncbi_accession}.exonset", "w") as o_file: + with gzip.open(f"{output_dir}/exonsets.gz", "wt") as o_file: esw = ExonSetWriter(o_file) for mg in mito_genes: esw.write( @@ -331,5 +333,4 @@ def main(ncbi_accession: str, output_dir: str): if __name__ == "__main__": args = parse_args() - main(args.accession, args.output_dir) diff --git a/sbin/seqrepo-download b/sbin/seqrepo-download deleted file mode 100755 index 0773030..0000000 --- a/sbin/seqrepo-download +++ /dev/null @@ -1,53 +0,0 @@ -#!/usr/bin/env bash - -# This script downloads SeqRepo into the given directory. -# Note: pulling data takes ~30 minutes and requires ~13 GB. -# Note: a container called seqrepo will be left behind. -# The name of the container can be changed by providing a third argument. - -set -e - -SEQREPO_VERSION=$1 -OUTPUT_DIR=$2 -# optional: -SEQREPO_CONTAINER_NAME=$3 - -if [ -z "$SEQREPO_VERSION" ] || [ -z "$OUTPUT_DIR" ] -then - echo 'Usage: sbin/seqrepo-download ' - exit 1 -else - echo "SeqRepo data for version $SEQREPO_VERSION will be available in $OUTPUT_DIR" -fi - -# Name of seqrepo container -if [ -z "$SEQREPO_CONTAINER_NAME" ] -then - SEQREPO_CONTAINER_NAME=seqrepo -fi - -if [[ $OUTPUT_DIR != /* ]] -then - echo 'Output directory must be an absolute path' - exit 1 -fi - -if [ ! -d "$OUTPUT_DIR" ]; then - echo "Directory $OUTPUT_DIR does not exist." - exit 1 -fi - -# Pull seqrepo image -docker pull biocommons/seqrepo:$SEQREPO_VERSION - -# Download seqrepo data using seqrepo image -if docker ps -aq -f name=$SEQREPO_CONTAINER_NAME -then - echo "Container called $SEQREPO_CONTAINER_NAME already exists. Skipping seqrepo data download." -else - docker run --name seqrepo biocommons/seqrepo:$SEQREPO_VERSION -fi - -# Copy seqrepo data into a local directory -echo "Copying seqrepo data into $OUTPUT_DIR ..." -docker run -it -v $OUTPUT_DIR:/output-dir --volumes-from $SEQREPO_CONTAINER_NAME:ro ubuntu bash -c 'cp -R /usr/local/share/seqrepo/* /output-dir' diff --git a/sbin/seqrepo-load b/sbin/seqrepo-load new file mode 100755 index 0000000..93942cf --- /dev/null +++ b/sbin/seqrepo-load @@ -0,0 +1,21 @@ +#!/usr/bin/env bash + +set -e + +seqrepo_root=$1 +seqrepo_version=$2 +sequence_dir=$3 +log_dir=$4 + +if [ -z "$seqrepo_root" ] || [ -z "$seqrepo_version" ] || [ -z "$sequence_dir" ] || [ -z "$log_dir" ] +then + echo 'Usage: sbin/seqrepo-load ' + exit 1 +fi + +## Load SeqRepo with new sequences +seqrepo --root-directory "$seqrepo_root" \ + load -n NCBI --instance-name "$seqrepo_version" \ + $sequence_dir/*.fna.gz \ + $sequence_dir/*.faa.gz 2>& 1 | \ + tee "$log_dir/seqrepo-load.log" diff --git a/sbin/uta-extract b/sbin/uta-extract new file mode 100755 index 0000000..2a60f3f --- /dev/null +++ b/sbin/uta-extract @@ -0,0 +1,43 @@ +#!/usr/bin/env bash + +# Extract data from NCBI files into intermediate files. + +set -e + +ncbi_dir=$1 +working_dir=$2 +log_dir=$3 + +if [ -z "$ncbi_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] +then + echo 'Usage: sbin/uta-extract ' + exit 1 +fi + +# genes +sbin/ncbi-parse-geneinfo $ncbi_dir/gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz | \ + gzip -c > "$working_dir/geneinfo.gz" 2>&1 | tee "$log_dir/ncbi-parse-geneinfo.log" + +# transcript protein associations +sbin/ncbi-parse-gene2refseq $ncbi_dir/gene/DATA/gene2accession.gz | gzip -c > "$working_dir/assocacs.gz" 2>&1 | \ + tee "$log_dir/ncbi-fetch-assoc-acs.log" + +# parse transcript info from GBFF input files +GBFF_files=$(ls $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz) +sbin/ncbi-parse-gbff "$GBFF_files" | gzip -c > "$working_dir/txinfo.gz" 2>&1 | \ + tee "$log_dir/ncbi-parse-gbff.log" + +# parse alignments from GFF input files +GFF_files=$(ls $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.gff.gz) +sbin/ncbi_parse_genomic_gff.py "$GFF_files" | gzip -c > "$working_dir/unfiltered_exonsets.gz" 2>&1 | \ + tee "$log_dir/ncbi-parse-genomic-gff.log" + +# filter transcripts +sbin/filter_exonset_transcripts.py --tx-info "$working_dir/txinfo.gz" --exonsets "$working_dir/unfiltered_exonsets.gz" \ + --missing-ids "$working_dir/filtered_tx_acs.txt" | gzip -c > "$working_dir/exonsets.gz" 2>&1 | \ + tee "$log_dir/filter_exonset_transcripts.log" + +# move fasta files into same dir +ln $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz $working_dir/ +ln $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz $working_dir/ +ln $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.fna.gz $working_dir/ diff --git a/sbin/uta-load b/sbin/uta-load new file mode 100755 index 0000000..c3da837 --- /dev/null +++ b/sbin/uta-load @@ -0,0 +1,77 @@ +#!/usr/bin/env bash + +# This script updates UTA and SeqRepo using NCBI files. +# source_uta_v is the UTA version before the update. +# ncbi_dir is where the script looks for NCBI data files. +# working_dir stores intermediate data files and the final database dump. +# log_dir stores log files. +# skip_load_genes, if truthy, will skip the gene loading step + +# Note that the uta loading code uses the seqrepo location defined in the conf files, under [sequences].seqrepo. + +set -euxo pipefail + +source_uta_v=$1 +ncbi_dir=$2 +working_dir=$3 +log_dir=$4 +skip_load_genes=$5 + +if [ -z "$source_uta_v" ] || [ -z "$ncbi_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] +then + echo 'Usage: uta-load ' + exit 1 +fi + +# set local variables and create working directories +loading_uta_v="uta" +mkdir -p "$log_dir" + +## Drop loading schema, and recreate +etc/scripts/delete-schema.sh "$loading_uta_v" +etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_uta_v" + +## for now set up Alembic for schema migrations +alembic -c etc/alembic.ini stamp edadb97f6502 +alembic -c etc/alembic.ini upgrade head + +# generate seqinfo files from exonsets (this step requires seqrepo) +sbin/exonset-to-seqinfo -o NCBI "$working_dir/exonsets.gz" | gzip -c > "$working_dir/seqinfo.gz" 2>&1 | \ + tee "$log_dir/exonset-to-seqinfo.log" + +# Filter out columns from assocacs file. +sbin/assoc-acs-merge "$working_dir/assocacs.gz" | gzip -c > "$working_dir/assoc-ac.gz" 2>&1 | \ + tee "$log_dir/assoc-acs-merge.log" + +# Load genes into gene table. +if [ "$skip_load_genes" = "true" ] +then + echo "Skipping load-geneinfo" +else + uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$working_dir/geneinfo.gz" 2>&1 | \ + tee "$log_dir/load-geneinfo.log" +fi + +# Load accessions into associated_accessions table. +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-assoc-ac "$working_dir/assoc-ac.gz" 2>&1 | \ + tee "$log_dir/load-assoc-ac.log" + +# Load transcript info into transcript and exon_set tables. +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$working_dir/txinfo.gz" 2>&1 | \ + tee "$log_dir/load-txinfo.log" + +# Load exon sets into into exon_set and exon tables. +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$working_dir/exonsets.gz" 2>&1 | \ + tee "$log_dir/load-exonsets.log" + +# Create cigar strings for all rows in tx_alt_exon_pairs_v view and update exon_aln table. +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | \ + tee "$log_dir/align-exons.log" + +# Load seqinfo? + +### run diff +sbin/uta-diff "$source_uta_v" "$loading_uta_v" + +### psql_dump +pg_dump -U uta_admin -h localhost -d uta -t "$loading_uta_v.gene" | gzip -c > "$working_dir/uta.pgd.gz" diff --git a/sbin/uta-update b/sbin/uta-update deleted file mode 100755 index 23d93af..0000000 --- a/sbin/uta-update +++ /dev/null @@ -1,67 +0,0 @@ -#!/usr/bin/env bash - -# This script runs the UTA update procedure. -# It updates the specified UTA and SeqRepo using the given NCBI files. -# It produces a postgres dump of the updated UTA database and an updated SeqRepo (updated in place). -# It expects to be run from the root of the uta repository. - -set -e - -# export environment variables for docker compose file -export NCBI_DIR=$1 -export SEQREPO_DIR=$2 -export WORKING_DIR=$3 -export UTA_VERSION=$4 -export SEQREPO_VERSION=$5 - -if [ -z "$NCBI_DIR" ] || [ -z "$SEQREPO_DIR" ] || [ -z "$WORKING_DIR" ] || [ -z "$UTA_VERSION" ] || [ -z "$SEQREPO_VERSION" ] -then - echo 'Usage: sbin/uta-update ' - exit 1 -else - echo "Updating UTA and SeqRepo using files in $NCBI_DIR and SeqRepo data in $SEQREPO_DIR" - echo "Starting from UTA version $UTA_VERSION and SeqRepo version $SEQREPO_VERSION" - echo "Logs and intermediate files will be available in $WORKING_DIR" -fi - -# Ensure directories are compatible with docker volume usage -if [[ $NCBI_DIR != /* ]] && [[ $NCBI_DIR != .* ]] -then - echo 'NCBI file directory must start with / or .' - exit 1 -fi - -if [[ $SEQREPO_DIR != /* ]] && [[ $SEQREPO_DIR != .* ]] -then - echo 'SeqRepo data directory must start with / or .' - exit 1 -fi - -if [[ $WORKING_DIR != /* ]] && [[ $WORKING_DIR != .* ]] -then - echo 'Working directory must start with / or .' - exit 1 -fi - -# Ensure directories exist. -if [ ! -d "$NCBI_DIR" ]; then - echo "Directory $NCBI_DIR does not exist." - exit 1 -fi - -if [ ! -d "$SEQREPO_DIR" ]; then - echo "Directory $SEQREPO_DIR does not exist." - exit 1 -fi - -if [ ! -d "$WORKING_DIR" ]; then - echo "Directory $WORKING_DIR does not exist." - exit 1 -fi - -# Build the UTA image. -docker build --target uta -t uta-update . - -# Bring up a UTA database and run the UTA update procedure. -# docker compose doesn't respect the container name specified in the compose file, so container name is specified here -docker compose run --rm --name uta-update uta-update diff --git a/tests/test_ncbi_process_mito.py b/tests/test_ncbi_process_mito.py index d0a1982..8beb535 100644 --- a/tests/test_ncbi_process_mito.py +++ b/tests/test_ncbi_process_mito.py @@ -193,7 +193,7 @@ def test_get_mito_genes(self): "alt_ac": "NC_012920.1", "alt_start": 1601, "alt_end": 1670, - "strand": "+", + "strand": 1, "transl_table": None, "transl_except": None, "pro_ac": None, @@ -212,7 +212,7 @@ def test_get_mito_genes(self): "alt_ac": "NC_012920.1", "alt_start": 4328, "alt_end": 4400, - "strand": "-", + "strand": -1, "transl_table": None, "transl_except": None, "pro_ac": None, @@ -237,7 +237,7 @@ def test_get_mito_genes(self): "alt_ac": "NC_012920.1", "alt_start": 7585, "alt_end": 8269, - "strand": "+", + "strand": 1, "transl_table": "2", "transl_except": None, "pro_ac": "YP_003024029.1", @@ -267,7 +267,7 @@ def test_get_mito_genes(self): "alt_ac": "NC_012920.1", "alt_start": 3306, "alt_end": 4262, - "strand": "+", + "strand": 1, "transl_table": "2", "transl_except": "(pos:4261..4262,aa:TERM)", "pro_ac": "YP_003024026.1", From 8431f274f919932204c374c64b168c4ed589ec39 Mon Sep 17 00:00:00 2001 From: Paige Date: Mon, 15 Apr 2024 07:20:14 -0700 Subject: [PATCH 32/54] feat(IPVC-2335): add the load-seqinfo step (#22) --- sbin/uta-load | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/sbin/uta-load b/sbin/uta-load index c3da837..f04c93b 100755 --- a/sbin/uta-load +++ b/sbin/uta-load @@ -64,12 +64,14 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo "$worki uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$working_dir/exonsets.gz" 2>&1 | \ tee "$log_dir/load-exonsets.log" +# Load seqinfo into the seq and seqanno tables. +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-seqinfo "$loading_dir/seqinfo.gz" 2>&1 | \ + tee "$logs_dir/load-seqinfo.log" + # Create cigar strings for all rows in tx_alt_exon_pairs_v view and update exon_aln table. uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | \ tee "$log_dir/align-exons.log" -# Load seqinfo? - ### run diff sbin/uta-diff "$source_uta_v" "$loading_uta_v" From 254176f45ac770cd212ddeab0d17ebf77ff184cf Mon Sep 17 00:00:00 2001 From: Paige Date: Wed, 17 Apr 2024 16:39:02 -0700 Subject: [PATCH 33/54] feat(IPVC-2331): add splign manual steps (#23) Co-authored-by: Shane Giles --- README.md | 13 +++++ docker-compose.yml | 12 +++++ loading/data/splign-manual/README.md | 2 +- loading/data/splign-manual/txdata.yaml | 32 +++++++++++- .../generate-loading-data | 31 +++++++---- sbin/uta-diff | 2 +- sbin/uta-splign-manual | 51 +++++++++++++++++++ src/uta/loading.py | 24 ++++----- 8 files changed, 143 insertions(+), 24 deletions(-) rename {loading/data/splign-manual => sbin}/generate-loading-data (77%) create mode 100755 sbin/uta-splign-manual diff --git a/README.md b/README.md index 2ade8c5..3acbe72 100644 --- a/README.md +++ b/README.md @@ -357,6 +357,19 @@ docker compose run seqrepo-load UTA_ETL_SKIP_GENE_LOAD=true docker compose run uta-load ``` +#### 2C. Manual splign transcripts +To load splign-manual transcripts, the workflow expects an input txdata.yaml file and splign alignments. Define this path +using the environment variable $UTA_SPLIGN_MANUAL_DIR. These file paths should exist: +- `$UTA_SPLIGN_MANUAL_DIR/splign-manual/txdata.yaml` +- `$UTA_SPLIGN_MANUAL_DIR/splign-manual/alignments/*.splign` + +[txdata.yaml](loading/data/splign-manual/txdata.yaml) defines the transcripts and their metadata. The [alignments dir](loading/data/splign-manual/alignments) contains the splign alignments. +To run the workflow: +``` +export UTA_SPLIGN_MANUAL_DIR=$(pwd)/loading/data/splign-manual/ +docker compose run splign-manual +``` + UTA has updated and the database has been dumped into a pgd file in `UTA_ETL_WORK_DIR`. SeqRepo has been updated in place. diff --git a/docker-compose.yml b/docker-compose.yml index 1247c09..b689645 100644 --- a/docker-compose.yml +++ b/docker-compose.yml @@ -60,3 +60,15 @@ services: - ${UTA_ETL_LOG_DIR}:/mito-extract/logs working_dir: /opt/repos/uta network_mode: host + splign-manual: + image: uta-update + command: sbin/uta-splign-manual 2024-02-20 ${UTA_ETL_OLD_UTA_VERSION} /uta-splign-manual/input /uta-splign-manual/work /uta-splign-manual/logs + depends_on: + uta: + condition: service_healthy + volumes: + - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo + - ${UTA_SPLIGN_MANUAL_DIR}:/uta-splign-manual/input + - ${UTA_ETL_WORK_DIR}:/uta-splign-manual/work + - ${UTA_ETL_LOG_DIR}:/uta-splign-manual/logs + network_mode: host diff --git a/loading/data/splign-manual/README.md b/loading/data/splign-manual/README.md index 3a4d62b..f364281 100644 --- a/loading/data/splign-manual/README.md +++ b/loading/data/splign-manual/README.md @@ -50,7 +50,7 @@ For a given RefSeq transcript (e.g., NM_000996.3), do the following: - Click on the gene id to go to the gene page (e.g., `6165`) - N.B. Strand is inferred from the orientation of aligned exons. -1. Enter the gene and CDS info in txdata.yaml +1. Enter the gene, geneID, and CDS info in txdata.yaml 1. Get the chromosome and coordinates from the gene page - From the "Genomic Context" section, note the chromosomal diff --git a/loading/data/splign-manual/txdata.yaml b/loading/data/splign-manual/txdata.yaml index 1e7498d..64ef14c 100644 --- a/loading/data/splign-manual/txdata.yaml +++ b/loading/data/splign-manual/txdata.yaml @@ -7,6 +7,7 @@ NM_000000.0: # transcript_accession cds: # CDS start and end, 1-based inclusive hgnc: # HGNC *symbol* genomic_region: # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/259291 + gene_id: # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/259291 NM_001025190.1: @@ -14,67 +15,80 @@ NM_001025190.1: cds: 1,3162 hgnc: MSLNL genomic_region: NC_000016.9 (819428..831996, complement) + gene_id: 401827 NM_006060.6: cds: 222,1781 hgnc: IKZF1 genomic_region: NC_000007.13 (50344378..50367358) , (50444231..50472799) + gene_id: 10320 NM_000769.4: cds: 26,1498 hgnc: CYP2C19 genomic_region: NC_000010.10 (96522463..96612671) + gene_id: 1557 NM_001807.4: cds: 17,2287 hgnc: CEL genomic_region: NC_000009.11 (135936741..135947250) + gene_id: 1056 NM_002116.7: cds: 85,1182 hgnc: HLA-A genomic_region: NC_000006.11 (29910247..29913661) + gene_id: 3105 NM_002122.3: cds: 54,821 hgnc: HLA-DQA1 genomic_region: NC_000006.11 (32605169..32612152) + gene_id: 3117 NM_006060.5: cds: 269,1828 hgnc: IKZF1 genomic_region: NC_000007.13 (50344378..50367358) , (50444231..50472799) + gene_id: 10320 NM_000996.3: cds: 65,397 hgnc: RPL35A genomic_region: NC_000003.11 (197677023..197682722) + gene_id: 6165 NM_001261826.2: cds: 293,3940 hgnc: AP3D1 genomic_region: NC_000019.9 (2100987..2151556, complement) + gene_id: 8943 NM_001355436.1: cds: 144,7130 hgnc: SPTB genomic_region: NC_000014.8 (65213001..65346604, complement) + gene_id: 6710 NM_001428.4: cds: 117,1421 hgnc: ENO1 genomic_region: NC_000001.10 (8921059..8939151, complement) + gene_id: 2023 NM_032589.2: - genomic_region: NM_032589.2 was permanently suppressed because currently there is support for the transcript but not for the protein. + # NM_032589.2 was permanently suppressed because currently there is support for the transcript but not for the protein. cds: 150,425 hgnc: DSCR8 genomic_region: NC_000021.8 (39493545..39528605) + gene_id: 84677 NM_176886.1: cds: 1,900 hgnc: TAS2R45 genomic_region: NW_003571050.1 (327525..328424, complement) + gene_id: 259291 @@ -90,6 +104,7 @@ NM_002457.4: cds: 28,15897 hgnc: MUC2 genomic_region: NC_000011.9 (1074875..1104417) + gene_id: 4583 # Case 2: overall low coverage and/or identity. @@ -99,6 +114,7 @@ NM_001277444.1: cds: 76,3411 hgnc: NBPF9 genomic_region: NC_000001.10 (144811743..144830407) + gene_id: 400818 # Case 3: high identity alignments but with large gaps. These @@ -110,18 +126,21 @@ NM_031421.4: cds: 131,2149 hgnc: TTC25 genomic_region: NC_000017.10 (40086888..40117669) + gene_id: 83538 NM_001349168.1: # Splign alignment has 159 nt unaligned exonic sequence. This is unusable. -Reece 2020-04-08 cds: 239,4762 hgnc: DCAF1 genomic_region: NC_000003.11 (51433298..51534018, complement) + gene_id: 9730 NM_001733.5: # Splign alignment has 232 nt unaligned exonic sequence. This is unusable. -Reece 2020-04-08 cds: 220,2337 hgnc: C1R genomic_region: NC_000012.11 (7241205..7245043, complement) , (7187513..7189412, complement) + gene_id: 715 # Transcript, gene, and genomic alignment info # cds start,end (in human, 1-based coordinates) and hgnc symbol @@ -132,53 +151,64 @@ NM_001038633.3: # transcript_accession cds: 893,1684 # CDS start and end, 1-based inclusive hgnc: RSPO1 # HGNC *symbol* genomic_region: NC_000001.10 (38076821..38100595, complement) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/284654 + gene_id: 284654 NM_005363.3: # transcript_accession cds: 208,1152 # CDS start and end, 1-based inclusive hgnc: MAGEA6 # HGNC *symbol* genomic_region: NC_000023.10 (151867245..151870814) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/4105 + gene_id: 4105 NM_006561.3: # transcript_accession cds: 161,1726 # CDS start and end, 1-based inclusive hgnc: CELF2 # HGNC *symbol* genomic_region: NC_000010.10 (10838851..11378674) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/10659 + gene_id: 10659 NM_001242908.1: # transcript_accession cds: 714,1505 # CDS start and end, 1-based inclusive hgnc: RSPO1 # HGNC *symbol* genomic_region: NC_000001.10 (38076821..38100595, complement) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/284654 + gene_id: 284654 NM_001242909.1: # transcript_accession cds: 474,1184 # CDS start and end, 1-based inclusive hgnc: RSPO1 # HGNC *symbol* genomic_region: NC_000001.10 (38076821..38100595, complement) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/259291 + gene_id: 284654 NM_001242910.1: # transcript_accession cds: 714,1316 # CDS start and end, 1-based inclusive hgnc: RSPO1 # HGNC *symbol* genomic_region: NC_000001.10 (38076821..38100595, complement) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/284654 + gene_id: 284654 NM_001012709.1: # transcript_accession cds: 46,912 # CDS start and end, 1-based inclusive hgnc: KRTAP5-4 # HGNC *symbol* genomic_region: NC_000011.9 (1642188..1643368, complement) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/387267 + gene_id: 387267 NM_001123068.1: # transcript_accession cds: 34,528 # CDS start and end, 1-based inclusive hgnc: COAS-2 # HGNC *symbol* genomic_region: NC_000001.10 (143767144..143767881, complement) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/644591 + gene_id: 644591 NM_130797.2: # transcript_accession cds: 130,2727 # CDS start and end, 1-based inclusive hgnc: DPPX # HGNC *symbol* genomic_region: NC_000007.13 (153584419..154264025) , (154400205..154685995) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/1804 + gene_id: 1804 NM_033060.2: # transcript_accession cds: 42,425 # CDS start and end, 1-based inclusive hgnc: KRTAP4-1 # HGNC *symbol* genomic_region: NC_000017.10 (39340352..39341147, complement) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/1804 + gene_id: 85285 NM_033060.3: # transcript_accession cds: 58,441 # CDS start and end, 1-based inclusive hgnc: KRTAP4-1 # HGNC *symbol* genomic_region: NC_000017.10 (39340352..39341163, complement) # from gene page e.g., https://www.ncbi.nlm.nih.gov/gene/1804 + gene_id: 85285 diff --git a/loading/data/splign-manual/generate-loading-data b/sbin/generate-loading-data similarity index 77% rename from loading/data/splign-manual/generate-loading-data rename to sbin/generate-loading-data index c904f3e..e02d3be 100755 --- a/loading/data/splign-manual/generate-loading-data +++ b/sbin/generate-loading-data @@ -6,6 +6,7 @@ import argparse import csv import gzip import logging +import os import sys import yaml @@ -20,7 +21,6 @@ method = "splign-manual" txinfo_fn = "txinfo.gz" exonset_fn = "exonset.gz" -txdata_fn = "txdata.yaml" def parse_args(argv): @@ -31,6 +31,16 @@ def parse_args(argv): "FILES", nargs="*" ) + ap.add_argument( + "--txdata", + required=True, + help="Path to txdata.yaml" + ) + ap.add_argument( + "--output-dir", + required=True, + help="Path to output directory" + ) opts = ap.parse_args(argv) return opts @@ -61,16 +71,19 @@ def parse_splign(fn, txdata): try: txd = txdata[tx_ac] except KeyError: - raise KeyError(f"{tx_ac}: no cds or gene_symbol info in {txdata_fn}") + raise KeyError(f"{tx_ac}: no cds or gene_symbol info in txdata") gene_symbol = txd["hgnc"] - gene_id = txd.get("gene_id") - if gene_symbol is None: - _logger.warning(f"No gene symbol in {txdata_fn} for {tx_ac}") + + gene_id = txd["gene_id"] + if gene_id is None: + msg = f"No gene id in txdata for {tx_ac}" + _logger.error(msg) + raise ValueError(msg) cds = txd["cds"] if cds is None: - _logger.warning(f"No CDS info {txdata_fn} for {tx_ac}; will be non-coding transcript") + _logger.warning(f"No CDS info txdata for {tx_ac}; will be non-coding transcript") cds_se_i = None else: cds = [int(i) for i in txd["cds"].split(",")] @@ -99,10 +112,10 @@ if __name__ == "__main__": opts = parse_args(sys.argv[1:]) - txdata = yaml.load(open(txdata_fn), Loader=yaml.SafeLoader) + txdata = yaml.load(open(opts.txdata), Loader=yaml.SafeLoader) - txinfo_out = uta.formats.txinfo.TxInfoWriter(gzip.open(txinfo_fn, "wt")) - exonset_out = uta.formats.exonset.ExonSetWriter(gzip.open(exonset_fn, "wt")) + txinfo_out = uta.formats.txinfo.TxInfoWriter(gzip.open(os.path.join(opts.output_dir, txinfo_fn), "wt")) + exonset_out = uta.formats.exonset.ExonSetWriter(gzip.open(os.path.join(opts.output_dir, exonset_fn), "wt")) for fn in opts.FILES: _logger.info("# " + fn) diff --git a/sbin/uta-diff b/sbin/uta-diff index 4caa50e..e65407a 100755 --- a/sbin/uta-diff +++ b/sbin/uta-diff @@ -66,7 +66,7 @@ if __name__ == "__main__": print("""UTA comparison: url={url}, s1={s1}, s2={s2} t: time taken (seconds) n1, n2: total number of rows in schemas s1 and s2 -nu1, nu2, c: number of rows unique to s1, unique to s2, and common to both +nu1, nu2, nc: number of rows unique to s1, unique to s2, and common to both cols: cols used for comparison """.format(url=url, s1=s1, s2=s2)) print(pt) diff --git a/sbin/uta-splign-manual b/sbin/uta-splign-manual new file mode 100755 index 0000000..2bc3e9b --- /dev/null +++ b/sbin/uta-splign-manual @@ -0,0 +1,51 @@ +#!/usr/bin/env bash + +# Process splign-manual alignments + +set -euxo pipefail + +seqrepo_version=$1 +source_uta_v=$2 +input_dir=$3 +working_dir=$4 +log_dir=$5 + +if [ -z "$seqrepo_version" ] || [ -z "$source_uta_v" ] || [ -z "$input_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] +then + echo 'Usage: sbin/uta-splign-manual ' + exit 1 +fi + +# set local variables and create working directories +loading_uta_v="uta" +working_dir="$working_dir/splign-manual" +log_dir="$log_dir/splign-manual" +mkdir -p "$log_dir" +mkdir -p "$working_dir" + +# Generate txinfo.gz and exonset.gz files +python sbin/generate-loading-data $input_dir/alignments/*.splign --txdata $input_dir/txdata.yaml --output-dir $working_dir 2>&1 | tee "$log_dir/generate-loading-data.log" + +# Generate fasta files +seqrepo export $(gzip -cdq $working_dir/txinfo.gz | cut -f2 | tail +2) --instance-name "$seqrepo_version" | gzip -c > $working_dir/seqs.fa.gz 2>&1 | tee "$log_dir/seqrepo-export.log" + +# Generate seqinfo.gz file +sbin/fasta-to-seqinfo -o NCBI $working_dir/seqs.fa.gz | gzip -c > $working_dir/seqinfo.gz 2>&1 | tee "$log_dir/fasta-to-seqinfo.log" + +# Load seqinfo +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-seqinfo $working_dir/seqinfo.gz 2>&1 | tee "$log_dir/load-seqinfo.log" + +# Load txinfo +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo $working_dir/txinfo.gz 2>&1 | tee "$log_dir/load-txinfo.log" + +# Load exonset +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset $working_dir/exonset.gz 2>&1 | tee "$log_dir/load-exonset.log" + +# Align exons +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | tee "$log_dir/align-exons.log" + +### run diff +sbin/uta-diff "$source_uta_v" "$loading_uta_v" 2>&1 | tee "$log_dir/uta-diff.log" + +### psql_dump +pg_dump -U uta_admin -h localhost -d uta -t "$loading_uta_v.gene" | gzip -c > "$working_dir/uta.pgd.gz" diff --git a/src/uta/loading.py b/src/uta/loading.py index 7ddd354..3939cbc 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -161,7 +161,7 @@ def analyze(session, opts, cf): ] for cmd in cmds: logger.info(cmd) - session.execute(cmd) + session.execute(text(cmd)) session.commit() @@ -214,31 +214,31 @@ def grant_permissions(session, opts, cf): sql = "select concat(schemaname,'.',tablename) as fqrn from pg_tables where schemaname='{schema}'".format( schema=schema) - rows = list(session.execute(sql)) + rows = list(session.execute(text(sql))) cmds += ["grant select on {fqrn} to PUBLIC".format( - fqrn=row["fqrn"]) for row in rows] + fqrn=row.fqrn) for row in rows] cmds += ["alter table {fqrn} owner to uta_admin".format( - fqrn=row["fqrn"]) for row in rows] + fqrn=row.fqrn) for row in rows] sql = "select concat(schemaname,'.',viewname) as fqrn from pg_views where schemaname='{schema}'".format( schema=schema) - rows = list(session.execute(sql)) + rows = list(session.execute(text(sql))) cmds += ["grant select on {fqrn} to PUBLIC".format( - fqrn=row["fqrn"]) for row in rows] + fqrn=row.fqrn) for row in rows] cmds += ["alter view {fqrn} owner to uta_admin".format( - fqrn=row["fqrn"]) for row in rows] + fqrn=row.fqrn) for row in rows] sql = "select concat(schemaname,'.',matviewname) as fqrn from pg_matviews where schemaname='{schema}'".format( schema=schema) - rows = list(session.execute(sql)) + rows = list(session.execute(text(sql))) cmds += ["grant select on {fqrn} to PUBLIC".format( - fqrn=row["fqrn"]) for row in rows] + fqrn=row.fqrn) for row in rows] cmds += ["alter materialized view {fqrn} owner to uta_admin".format( - fqrn=row["fqrn"]) for row in rows] + fqrn=row.fqrn) for row in rows] for cmd in sorted(cmds): logger.info(cmd) - session.execute(cmd) + session.execute(text(cmd)) session.commit() @@ -761,7 +761,7 @@ def refresh_matviews(session, opts, cf): for cmd in cmds: logger.info(cmd) - session.execute(cmd) + session.execute(text(cmd)) session.commit() From 5432818f239fe12c3f32f71e9b5dca12e204c0af Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Thu, 18 Apr 2024 15:14:06 -0600 Subject: [PATCH 34/54] feat(IPVC-2264) add gene_id to UTA models (#24) --- .gitignore | 1 - etc/scripts/create-new-schema.sh | 3 +- misc/gene-update/backfill_gene_id.py | 116 +++++++ misc/gene-update/upgrade-uta-schema.sh | 55 +++ sbin/ncbi-parse-geneinfo | 2 - sbin/uta-diff | 2 +- sbin/uta-extract | 6 +- sbin/uta-load | 5 +- ...6de7_add_gene_id_to_gene_and_transcript.py | 44 +++ ...et_gene_id_and_primary_and_foreign_keys.py | 318 ++++++++++++++++++ src/uta/loading.py | 40 +-- src/uta/models.py | 12 +- tests/test_uta_loading.py | 30 +- tests/test_uta_models.py | 4 +- 14 files changed, 587 insertions(+), 51 deletions(-) create mode 100644 misc/gene-update/backfill_gene_id.py create mode 100644 misc/gene-update/upgrade-uta-schema.sh create mode 100644 src/alembic/versions/595a586e6de7_add_gene_id_to_gene_and_transcript.py create mode 100644 src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py diff --git a/.gitignore b/.gitignore index b0a2906..d7fefb9 100644 --- a/.gitignore +++ b/.gitignore @@ -57,7 +57,6 @@ misc/ncbi-geneinfo/ncbi.geneinfo.tsv.gz misc/partial-alignments sdist tmp -update/ncbi/ venv nosetests.xml .vscode diff --git a/etc/scripts/create-new-schema.sh b/etc/scripts/create-new-schema.sh index 5200e8d..d13100a 100755 --- a/etc/scripts/create-new-schema.sh +++ b/etc/scripts/create-new-schema.sh @@ -21,5 +21,4 @@ pg_dump -U uta_admin -h localhost -d uta -n "$source_uta_v" | \ # create new schema gzip -cdq $dumps_dir/"$source_uta_v".pgd.gz | \ sbin/pg-dump-schema-rename "$source_uta_v" "$dest_uta_v" | \ - sbin/pg-dump-schema-rename "uta_1_1" "$dest_uta_v" | \ - psql -U uta_admin -h localhost -d uta -aeE \ No newline at end of file + psql -U uta_admin -h localhost -d uta -aeE diff --git a/misc/gene-update/backfill_gene_id.py b/misc/gene-update/backfill_gene_id.py new file mode 100644 index 0000000..5961cfd --- /dev/null +++ b/misc/gene-update/backfill_gene_id.py @@ -0,0 +1,116 @@ +import argparse +import logging + +from datetime import datetime +from sqlalchemy.orm import Session +from sqlalchemy import text + +import uta +from uta.models import Gene, Transcript + + +logger = None +n = 50000 + + +def backfill_gene(uta_session: Session, gene_update_file: str) -> None: + logger.info("Dropping gene table contents") + uta_session.execute(text("DELETE FROM uta.gene;")) + uta_session.commit() + + logger.info(f"Back filling gene table from {gene_update_file}") + now_ts = datetime.now() + i = 0 + new_genes = [] + with open(gene_update_file) as f: + for line in f: + if line.startswith("gene_id"): + continue + + if i % n == 0: + if i > 0: + logger.info(f"Bulk inserting {len(new_genes)} genes") + uta_session.bulk_save_objects(new_genes) + uta_session.commit() + logger.info(f"Processing chunk {int(i/n) + 1}") + new_genes = [] + + gene_id, hgnc, maploc, desc, summary, aliases, added = line.rstrip("\r\n").split("\t") + # set timestamp from file string, if empty set to now. + if added == "": + added_ts = now_ts + else: + added_ts = datetime.strptime(added, "%Y-%m-%d %H:%M:%S.%f") + + # clean up aliases + aliases = aliases.replace("{", "").replace("}", "") + if aliases == "-": + aliases = None + + gene = Gene( + gene_id=gene_id, + hgnc=hgnc, + maploc=maploc if maploc else None, + descr=desc if desc else None, + summary=summary if desc else None, + aliases=aliases if aliases else None, + added=added_ts, + ) + i += 1 + new_genes.append(gene) + + logger.info(f"Bulk inserting {len(new_genes)} genes") + uta_session.bulk_save_objects(new_genes) + uta_session.commit() + logger.info(f"Inserted {i} total genes") + + +def backfill_transcript(uta_session: Session, transcript_update_file: str) -> None: + logger.info("Backfilling gene_id in transcript table") + tx_ac_to_gene_id = {} + + logger.info(f"Reading transcript to gene id mappings from {transcript_update_file}") + with open(transcript_update_file) as f: + for line in f: + if line.startswith("origin"): + continue + _, tx_ac, gene_id, _ = line.rstrip("\r\n").split("\t") + tx_ac_to_gene_id[tx_ac] = gene_id + logger.info(f" - {len(tx_ac_to_gene_id)} mappings read") + + i = 0 + txs = [] + for tx_ac, gene_id in tx_ac_to_gene_id.items(): + if i % n == 0: + if i > 0: + logger.info(f"Updating {len(txs)} transcripts") + uta_session.flush() + + logger.info(f"Processing chunk {int(i/n) + 1}") + txs = [] + + tx = uta_session.query(Transcript).filter(Transcript.ac == tx_ac).one() + tx.gene_id = gene_id + txs.append(tx) + i += 1 + + logger.info(f"Updating {len(txs)} transcripts") + uta_session.flush() + uta_session.commit() + + +if __name__ == "__main__": + parser = argparse.ArgumentParser(description="Backfill gene_id in gene and transcript tables") + parser.add_argument("db_url", help="URL of the UTA database") + parser.add_argument("gene_update_file", type=str, help="File containing gene_id updates for gene table") + parser.add_argument("transcript_update_file", type=str, help="File containing gene_id updates for transcript table") + args = parser.parse_args() + + logging.basicConfig(level=logging.INFO, format='%(asctime)s %(message)s') + logger = logging.getLogger("backfill_gene_id") + + session = uta.connect(args.db_url) + + backfill_gene(session, args.gene_update_file) + backfill_transcript(session, args.transcript_update_file) + session.close() diff --git a/misc/gene-update/upgrade-uta-schema.sh b/misc/gene-update/upgrade-uta-schema.sh new file mode 100644 index 0000000..0671780 --- /dev/null +++ b/misc/gene-update/upgrade-uta-schema.sh @@ -0,0 +1,55 @@ +#!/bin/bash + +# This script is used to upgrade older UTA schemas (specifically uta_20210129b) to a newer version. +# Part of this upgrade is to introduce gene_id to the gene and transcript tables. The columns are +# added with a Alembic migration. Then a data migration to back fill the new columns. Then a second +# Alembic migration to add the constraints to the columns and update primary and foreign keys. + +if [ "$#" -lt 1 ] +then + echo "error: too few arguments, you provided $#, 1 required" + echo "usage: upgrade-uta-schema.sh " + exit 1 +fi + +set -euxo pipefail + +source_uta_v="uta_20210129b" +working_uta_v="uta" +dest_uta_v=$1 +dumps_dir="/workdir/dumps" +mkdir -p $dumps_dir + +## setup working uta schema +# delete schema if exists +psql -h localhost -U uta_admin -d uta -c "DROP SCHEMA IF EXISTS $working_uta_v CASCADE;" + +# dump source version +pg_dump -U uta_admin -h localhost -d uta -n "$source_uta_v" | \ + gzip -c > $dumps_dir/"$source_uta_v".pgd.gz + +# create new schema +gzip -cdq $dumps_dir/"$source_uta_v".pgd.gz | \ + sbin/pg-dump-schema-rename "$source_uta_v" "$working_uta_v" | \ + sbin/pg-dump-schema-rename "uta_1_1" "$working_uta_v" | \ + psql -U uta_admin -h localhost -d uta -aeE + +## upgrade working uta schema +# set initial Alembic migration so it is not ran. +alembic -c etc/alembic.ini stamp edadb97f6502 + +# run Alembic migration to add gene_id to gene and transcript tables +alembic -c etc/alembic.ini upgrade 595a586e6de7 + +# run data migration to back fill gene_id +python misc/gene-update/backfill_gene_id.py \ + postgresql://uta_admin:@localhost/uta \ + /workdir/backfill/gene_update.tsv \ + /workdir/backfill/transcript_update.tsv + +# run Alembic migrations to add constraints and update existing views +alembic -c etc/alembic.ini upgrade head + +## Rename schema to destination schema name +psql -h localhost -U uta_admin -d uta -c "DROP SCHEMA IF EXISTS $dest_uta_v CASCADE;" +psql -h localhost -U uta_admin -d uta -c "ALTER SCHEMA uta RENAME TO $dest_uta_v"; diff --git a/sbin/ncbi-parse-geneinfo b/sbin/ncbi-parse-geneinfo index 3dd2534..bc33aad 100755 --- a/sbin/ncbi-parse-geneinfo +++ b/sbin/ncbi-parse-geneinfo @@ -30,8 +30,6 @@ if __name__ == "__main__": giw = GeneInfoWriter(sys.stdout) for rec in gi_in: - if rec["symbol_from_nomenclature_authority"] == "-": - continue gi = GeneInfo( tax_id=rec["tax_id"], gene_symbol=rec["symbol"], diff --git a/sbin/uta-diff b/sbin/uta-diff index e65407a..7b35158 100755 --- a/sbin/uta-diff +++ b/sbin/uta-diff @@ -14,7 +14,7 @@ cmp_cols = collections.defaultdict(lambda: ['*']) cmp_cols.update({ "associated_accessions": "tx_ac pro_ac origin".split(), "exon_aln": "exon_aln_id tx_exon_id alt_exon_id cigar added".split(), - "gene": "hgnc".split(), + "gene": "gene_id".split(), "transcript": "ac".split(), }) diff --git a/sbin/uta-extract b/sbin/uta-extract index 2a60f3f..a8d3d60 100755 --- a/sbin/uta-extract +++ b/sbin/uta-extract @@ -38,6 +38,6 @@ sbin/filter_exonset_transcripts.py --tx-info "$working_dir/txinfo.gz" --exonsets tee "$log_dir/filter_exonset_transcripts.log" # move fasta files into same dir -ln $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz $working_dir/ -ln $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz $working_dir/ -ln $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.fna.gz $working_dir/ +cp -f $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz $working_dir/ +cp -f $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz $working_dir/ +cp -f $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.fna.gz $working_dir/ diff --git a/sbin/uta-load b/sbin/uta-load index f04c93b..df0f3db 100755 --- a/sbin/uta-load +++ b/sbin/uta-load @@ -32,7 +32,6 @@ etc/scripts/delete-schema.sh "$loading_uta_v" etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_uta_v" ## for now set up Alembic for schema migrations -alembic -c etc/alembic.ini stamp edadb97f6502 alembic -c etc/alembic.ini upgrade head # generate seqinfo files from exonsets (this step requires seqrepo) @@ -65,8 +64,8 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset "$work tee "$log_dir/load-exonsets.log" # Load seqinfo into the seq and seqanno tables. -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-seqinfo "$loading_dir/seqinfo.gz" 2>&1 | \ - tee "$logs_dir/load-seqinfo.log" +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-seqinfo "$working_dir/seqinfo.gz" 2>&1 | \ + tee "$log_dir/load-seqinfo.log" # Create cigar strings for all rows in tx_alt_exon_pairs_v view and update exon_aln table. uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | \ diff --git a/src/alembic/versions/595a586e6de7_add_gene_id_to_gene_and_transcript.py b/src/alembic/versions/595a586e6de7_add_gene_id_to_gene_and_transcript.py new file mode 100644 index 0000000..06156bb --- /dev/null +++ b/src/alembic/versions/595a586e6de7_add_gene_id_to_gene_and_transcript.py @@ -0,0 +1,44 @@ +"""add gene_id to gene and transcript + +Revision ID: 595a586e6de7 +Revises: a697b584f699 +Create Date: 2024-04-10 19:47:43.685672 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa + + +# revision identifiers, used by Alembic. +revision: str = '595a586e6de7' +down_revision: Union[str, None] = 'a697b584f699' +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.add_column('gene', sa.Column('gene_id', sa.Text(), nullable=True), schema='uta') + op.add_column('gene', sa.Column('type', sa.Text(), nullable=True), schema='uta') + op.add_column('gene', sa.Column('xrefs', sa.Text(), nullable=True), schema='uta') + op.add_column('transcript', sa.Column('gene_id', sa.Text(), nullable=True), schema='uta') + # ### end Alembic commands ### + + # ### commands to drop existing primary key on gene table ### + op.drop_constraint('gene_pkey', 'gene', schema='uta') + # ### end of commands ### + + +def downgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.drop_column('transcript', 'gene_id', schema='uta') + op.drop_column('gene', 'xrefs', schema='uta') + op.drop_column('gene', 'type', schema='uta') + op.drop_column('gene', 'gene_id', schema='uta') + # ### end Alembic commands ### + + # ### commands to add primary key on gene table ### + op.create_primary_key('gene_pkey', 'gene', ['hgnc'], schema='uta') + # ### end of commands ### diff --git a/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py b/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py new file mode 100644 index 0000000..094d05a --- /dev/null +++ b/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py @@ -0,0 +1,318 @@ +"""set gene_id and primary and foreign keys + +Revision ID: f85dd97bd9f5 +Revises: 595a586e6de7 +Create Date: 2024-04-10 22:14:14.055461 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa + + +# revision identifiers, used by Alembic. +revision: str = 'f85dd97bd9f5' +down_revision: Union[str, None] = '595a586e6de7' +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.alter_column( + "gene", "gene_id", existing_type=sa.TEXT(), nullable=False, schema="uta" + ) + op.create_primary_key("gene_pkey", "gene", ["gene_id"], schema="uta") + op.create_index(op.f("ix_uta_gene_hgnc"), "gene", ["hgnc"], unique=False, schema="uta") + op.alter_column( + "transcript", "gene_id", existing_type=sa.TEXT(), nullable=False, schema="uta" + ) + op.create_index( + op.f("ix_uta_transcript_gene_id"), + "transcript", + ["gene_id"], + unique=False, + schema="uta", + ) + op.create_foreign_key( + "fk_uta_transcript_gene_gene_id", + "transcript", + "gene", + ["gene_id"], + ["gene_id"], + source_schema="uta", + referent_schema="uta", + onupdate="RESTRICT", + ondelete="RESTRICT", + ) + # ### end Alembic commands ### + + # ### handle first part of hgnc -> gene_symbol column rename ### + op.add_column("gene", sa.Column("symbol", sa.Text(), nullable=True), schema="uta") + op.create_index(op.f("ix_uta_gene_symbol"), "gene", ["symbol"], unique=False, schema="uta") + op.execute("UPDATE gene SET symbol = hgnc;") + # ### end of hgnc -> gene_symbol column rename ### + + # ### updates required to existing views needed to drop hgnc from transcript. ### + op.execute("DROP VIEW IF EXISTS tx_similarity_v CASCADE;") + op.execute("DROP MATERIALIZED VIEW IF EXISTS tx_def_summary_mv CASCADE;") + op.execute("DROP VIEW IF EXISTS tx_def_summary_dv CASCADE;") + op.execute("DROP MATERIALIZED VIEW IF EXISTS tx_exon_set_summary_mv CASCADE;") + op.execute("DROP VIEW IF EXISTS tx_exon_set_summary_dv CASCADE;") + op.execute("DROP VIEW IF EXISTS tx_exon_aln_v CASCADE;") + op.execute("DROP VIEW IF EXISTS tx_alt_exon_pairs_v CASCADE;") + op.execute("DROP VIEW IF EXISTS _discontiguous_tx CASCADE;") + op.execute(""" + CREATE VIEW _discontiguous_tx AS + SELECT g.symbol, + g.symbol as hgnc, + g.gene_id, + es.exon_set_id, + es.tx_ac, + format('[%s-%s]'::text, e1.end_i, e2.start_i) AS gap, + e1.exon_id AS e1_exon_id, + e1.ord AS e1_ord, + e1.start_i AS e1_start_i, + e1.end_i AS e1_end_i, + e2.exon_id AS e2_exon_id, + e2.ord AS e2_ord, + e2.start_i AS e2_start_i, + e2.end_i AS e2_end_i + FROM exon_set es + JOIN transcript t ON es.tx_ac = t.ac + JOIN gene as g ON t.gene_id = g.gene_id + JOIN exon e1 ON es.exon_set_id = e1.exon_set_id + JOIN exon e2 ON es.exon_set_id = e2.exon_set_id AND e2.ord = (e1.ord + 1) AND e1.end_i <> e2.start_i + WHERE es.alt_aln_method = 'transcript'::text; + """) + op.execute(""" + CREATE VIEW tx_alt_exon_pairs_v AS + SELECT g.symbol, g.symbol as hgnc, g.gene_id,TES.exon_SET_id AS tes_exon_SET_id, + AES.exon_SET_id AS aes_exon_SET_id, TES.tx_ac AS tx_ac,AES.alt_ac AS alt_ac, + AES.alt_strand,AES.alt_aln_method, TEX.ORD AS ORD,TEX.exon_id AS tx_exon_id, + AEX.exon_id AS alt_exon_id, TEX.start_i AS tx_start_i,TEX.END_i AS tx_END_i, + AEX.start_i AS alt_start_i, AEX.END_i AS alt_END_i, EA.exon_aln_id,EA.cigar + FROM exon_SET tes + JOIN transcript t ON tes.tx_ac=t.ac + JOIN gene g ON t.gene_id=g.gene_id + JOIN exon_set aes ON tes.tx_ac=aes.tx_ac AND tes.alt_aln_method='transcript' AND aes.alt_aln_method!='transcript' + JOIN exon tex ON tes.exon_SET_id=tex.exon_SET_id + JOIN exon aex ON aes.exon_SET_id=aex.exon_SET_id AND tex.ORD=aex.ORD + LEFT JOIN exon_aln ea ON ea.tx_exon_id=tex.exon_id AND ea.alt_exon_id=AEX.exon_id; + """) + op.execute(""" + CREATE VIEW tx_exon_aln_v AS + SELECT G.symbol, G.symbol AS hgnc, G.gene_id, T.ac as tx_ac, AES.alt_ac, + AES.alt_aln_method,AES.alt_strand, TE.ord, TE.start_i as tx_start_i, + TE.end_i as tx_end_i, AE.start_i as alt_start_i, AE.end_i as alt_end_i, + EA.cigar, EA.tx_aseq, EA.alt_aseq, TES.exon_set_id AS tx_exon_set_id, + AES.exon_set_id as alt_exon_set_id, TE.exon_id as tx_exon_id, + AE.exon_id as alt_exon_id, EA.exon_aln_id + FROM transcript T + JOIN gene G ON T.gene_id=G.gene_id + JOIN exon_set TES ON T.ac=TES.tx_ac AND TES.alt_aln_method ='transcript' + JOIN exon_set AES on T.ac=AES.tx_ac and AES.alt_aln_method!='transcript' + JOIN exon TE ON TES.exon_set_id=TE.exon_set_id + JOIN exon AE ON AES.exon_set_id=AE.exon_set_id AND TE.ord=AE.ord + LEFT JOIN exon_aln EA ON TE.exon_id=EA.tx_exon_id AND AE.exon_id=EA.alt_exon_id; + """) + op.execute(""" + CREATE VIEW tx_exon_set_summary_dv AS + SELECT G.symbol, G.symbol as hgnc, G.gene_id, cds_md5, es_fingerprint, tx_ac, alt_ac, + alt_aln_method, alt_strand, exon_set_id, n_exons, se_i, starts_i, ends_i, lengths + FROM transcript T + JOIN gene G ON T.gene_id=G.gene_id + JOIN exon_set_exons_fp_mv ESE ON T.ac=ESE.tx_ac; + """) + op.execute(""" + CREATE MATERIALIZED VIEW tx_exon_set_summary_mv AS SELECT * FROM tx_exon_set_summary_dv WITH NO DATA; + CREATE INDEX tx_exon_set_summary_mv_cds_md5_ix ON tx_exon_set_summary_mv(cds_md5); + CREATE INDEX tx_exon_set_summary_mv_es_fingerprint_ix ON tx_exon_set_summary_mv(es_fingerprint); + CREATE INDEX tx_exon_set_summary_mv_tx_ac_ix ON tx_exon_set_summary_mv(tx_ac); + CREATE INDEX tx_exon_set_summary_mv_alt_ac_ix ON tx_exon_set_summary_mv(alt_ac); + CREATE INDEX tx_exon_set_summary_mv_alt_aln_method_ix ON tx_exon_set_summary_mv(alt_aln_method); + GRANT SELECT ON tx_exon_set_summary_mv TO public; + REFRESH MATERIALIZED VIEW tx_exon_set_summary_mv; + """) + op.execute(""" + CREATE VIEW tx_def_summary_dv AS + SELECT TESS.exon_set_id, TESS.tx_ac, TESS.alt_ac, TESS.alt_aln_method, TESS.alt_strand, + TESS.symbol, TESS.hgnc, TESS.gene_id, TESS.cds_md5, TESS.es_fingerprint, CEF.cds_es_fp, + CEF.cds_exon_lengths_fp, TESS.n_exons, TESS.se_i, CEF.cds_se_i, TESS.starts_i, + TESS.ends_i, TESS.lengths, T.cds_start_i, T.cds_end_i, CEF.cds_start_exon, CEF.cds_end_exon + FROM tx_exon_set_summary_mv TESS + JOIN transcript T ON TESS.tx_ac=T.ac + LEFT JOIN _cds_exons_fp_v CEF ON TESS.exon_set_id=CEF.exon_set_id + WHERE TESS.alt_aln_method = 'transcript'; + """) + op.execute(""" + CREATE MATERIALIZED VIEW tx_def_summary_mv AS SELECT * FROM tx_def_summary_dv WITH NO DATA; + CREATE INDEX tx_def_summary_mv_tx_ac ON tx_def_summary_mv (tx_ac); + CREATE INDEX tx_def_summary_mv_alt_ac ON tx_def_summary_mv (alt_ac); + CREATE INDEX tx_def_summary_mv_alt_aln_method ON tx_def_summary_mv (alt_aln_method); + CREATE INDEX tx_def_summary_mv_hgnc ON tx_def_summary_mv (hgnc); + CREATE INDEX tx_def_summary_mv_symbol ON tx_def_summary_mv (symbol); + CREATE INDEX tx_def_summary_mv_gene_id ON tx_def_summary_mv (gene_id); + REFRESH MATERIALIZED VIEW tx_def_summary_mv; + """) + op.execute(""" + CREATE VIEW tx_similarity_v AS + SELECT DISTINCT + D1.tx_ac as tx_ac1, D2.tx_ac as tx_ac2, + D1.symbol = D2.symbol as symbol_eq, + D1.cds_md5=D2.cds_md5 as cds_eq, + D1.es_fingerprint=D2.es_fingerprint as es_fp_eq, + D1.cds_es_fp=D2.cds_es_fp as cds_es_fp_eq, + D1.cds_exon_lengths_fp=D2.cds_exon_lengths_fp as cds_exon_lengths_fp_eq + FROM tx_def_summary_mv D1 + JOIN tx_def_summary_mv D2 on (D1.tx_ac != D2.tx_ac + and (D1.symbol=D2.symbol + or D1.cds_md5=D2.cds_md5 + or D1.es_fingerprint=D2.es_fingerprint + or D1.cds_es_fp=D2.cds_es_fp + or D1.cds_exon_lengths_fp=D2.cds_exon_lengths_fp + )); + """) + # ### end of updates to existing views ### + + # ### drop hgnc from transcript ### + op.drop_column('transcript', 'hgnc', schema='uta') + # ### end Alembic commands ### + + +def downgrade() -> None: + # ### updates to views to add hgnc to transcript ### + op.add_column('transcript', sa.Column('hgnc', sa.Text(), nullable=True), schema='uta') + # ### end of updates to transcript ### + + # ### commands to downgrade views before adding hgnc to transcript ### + op.execute("DROP VIEW IF EXISTS tx_similarity_v CASCADE;") + op.execute("DROP MATERIALIZED VIEW IF EXISTS tx_def_summary_mv CASCADE;") + op.execute("DROP VIEW IF EXISTS tx_def_summary_dv CASCADE;") + op.execute("DROP MATERIALIZED VIEW IF EXISTS tx_exon_set_summary_mv CASCADE;") + op.execute("DROP VIEW IF EXISTS tx_exon_set_summary_dv CASCADE;") + op.execute("DROP VIEW IF EXISTS tx_exon_aln_v CASCADE;") + op.execute("DROP VIEW IF EXISTS tx_alt_exon_pairs_v CASCADE;") + op.execute("DROP VIEW IF EXISTS _discontiguous_tx CASCADE;") + op.execute(""" + CREATE VIEW _discontiguous_tx AS + SELECT t.hgnc, + es.exon_set_id, + es.tx_ac, + format('[%s-%s]'::text, e1.end_i, e2.start_i) AS gap, + e1.exon_id AS e1_exon_id, + e1.ord AS e1_ord, + e1.start_i AS e1_start_i, + e1.end_i AS e1_end_i, + e2.exon_id AS e2_exon_id, + e2.ord AS e2_ord, + e2.start_i AS e2_start_i, + e2.end_i AS e2_end_i + FROM exon_set es + JOIN transcript t ON es.tx_ac = t.ac + JOIN exon e1 ON es.exon_set_id = e1.exon_set_id + JOIN exon e2 ON es.exon_set_id = e2.exon_set_id AND e2.ord = (e1.ord + 1) AND e1.end_i <> e2.start_i + WHERE es.alt_aln_method = 'transcript'::text; + """) + op.execute(""" + CREATE VIEW tx_alt_exon_pairs_v AS + SELECT t.hgnc,TES.exon_SET_id AS tes_exon_SET_id,AES.exon_SET_id AS aes_exon_SET_id, + TES.tx_ac AS tx_ac,AES.alt_ac AS alt_ac,AES.alt_strand,AES.alt_aln_method, + TEX.ORD AS ORD,TEX.exon_id AS tx_exon_id,AEX.exon_id AS alt_exon_id, + TEX.start_i AS tx_start_i,TEX.END_i AS tx_END_i, AEX.start_i AS alt_start_i,AEX.END_i AS alt_END_i, + EA.exon_aln_id,EA.cigar + FROM exon_SET tes + JOIN transcript t ON tes.tx_ac=t.ac + JOIN exon_set aes ON tes.tx_ac=aes.tx_ac AND tes.alt_aln_method='transcript' AND aes.alt_aln_method!='transcript' + JOIN exon tex ON tes.exon_SET_id=tex.exon_SET_id + JOIN exon aex ON aes.exon_SET_id=aex.exon_SET_id AND tex.ORD=aex.ORD + LEFT JOIN exon_aln ea ON ea.tx_exon_id=tex.exon_id AND ea.alt_exon_id=AEX.exon_id; + """) + op.execute(""" + CREATE VIEW tx_exon_aln_v AS + SELECT T.hgnc,T.ac as tx_ac,AES.alt_ac,AES.alt_aln_method,AES.alt_strand, + TE.ord, TE.start_i as tx_start_i,TE.end_i as tx_end_i, + AE.start_i as alt_start_i, AE.end_i as alt_end_i, + EA.cigar, EA.tx_aseq, EA.alt_aseq, + TES.exon_set_id AS tx_exon_set_id,AES.exon_set_id as alt_exon_set_id, + TE.exon_id as tx_exon_id, AE.exon_id as alt_exon_id, + EA.exon_aln_id + FROM transcript T + JOIN exon_set TES ON T.ac=TES.tx_ac AND TES.alt_aln_method ='transcript' + JOIN exon_set AES on T.ac=AES.tx_ac and AES.alt_aln_method!='transcript' + JOIN exon TE ON TES.exon_set_id=TE.exon_set_id + JOIN exon AE ON AES.exon_set_id=AE.exon_set_id AND TE.ord=AE.ord + LEFT JOIN exon_aln EA ON TE.exon_id=EA.tx_exon_id AND AE.exon_id=EA.alt_exon_id; + """) + op.execute(""" + CREATE VIEW tx_exon_set_summary_dv AS + SELECT T.hgnc,cds_md5,es_fingerprint,tx_ac,alt_ac,alt_aln_method,alt_strand,exon_set_id,n_exons,se_i,starts_i,ends_i,lengths + FROM transcript T + JOIN exon_set_exons_fp_mv ESE ON T.ac=ESE.tx_ac; + """) + op.execute(""" + CREATE MATERIALIZED VIEW tx_exon_set_summary_mv AS SELECT * FROM tx_exon_set_summary_dv WITH NO DATA; + CREATE INDEX tx_exon_set_summary_mv_cds_md5_ix ON tx_exon_set_summary_mv(cds_md5); + CREATE INDEX tx_exon_set_summary_mv_es_fingerprint_ix ON tx_exon_set_summary_mv(es_fingerprint); + CREATE INDEX tx_exon_set_summary_mv_tx_ac_ix ON tx_exon_set_summary_mv(tx_ac); + CREATE INDEX tx_exon_set_summary_mv_alt_ac_ix ON tx_exon_set_summary_mv(alt_ac); + CREATE INDEX tx_exon_set_summary_mv_alt_aln_method_ix ON tx_exon_set_summary_mv(alt_aln_method); + GRANT SELECT ON tx_exon_set_summary_mv TO public; + REFRESH MATERIALIZED VIEW tx_exon_set_summary_mv; + """) + op.execute(""" + CREATE VIEW tx_def_summary_dv AS + SELECT TESS.exon_set_id, TESS.tx_ac, TESS.alt_ac, TESS.alt_aln_method, TESS.alt_strand, + TESS.hgnc, TESS.cds_md5, TESS.es_fingerprint, CEF.cds_es_fp, + CEF.cds_exon_lengths_fp, TESS.n_exons, TESS.se_i, CEF.cds_se_i, TESS.starts_i, + TESS.ends_i, TESS.lengths, T.cds_start_i, T.cds_end_i, CEF.cds_start_exon, CEF.cds_end_exon + FROM tx_exon_set_summary_mv TESS + JOIN transcript T ON TESS.tx_ac=T.ac + LEFT JOIN _cds_exons_fp_v CEF ON TESS.exon_set_id=CEF.exon_set_id + WHERE TESS.alt_aln_method = 'transcript'; + """) + op.execute(""" + CREATE MATERIALIZED VIEW tx_def_summary_mv AS SELECT * FROM tx_def_summary_dv WITH NO DATA; + CREATE INDEX tx_def_summary_mv_tx_ac ON tx_def_summary_mv (tx_ac); + CREATE INDEX tx_def_summary_mv_alt_ac ON tx_def_summary_mv (alt_ac); + CREATE INDEX tx_def_summary_mv_alt_aln_method ON tx_def_summary_mv (alt_aln_method); + CREATE INDEX tx_def_summary_mv_hgnc ON tx_def_summary_mv (hgnc); + REFRESH MATERIALIZED VIEW tx_def_summary_mv; + """) + op.execute(""" + CREATE VIEW tx_similarity_v AS + SELECT DISTINCT + D1.tx_ac as tx_ac1, D2.tx_ac as tx_ac2, + D1.hgnc = D2.hgnc as hgnc_eq, + D1.cds_md5=D2.cds_md5 as cds_eq, + D1.es_fingerprint=D2.es_fingerprint as es_fp_eq, + D1.cds_es_fp=D2.cds_es_fp as cds_es_fp_eq, + D1.cds_exon_lengths_fp=D2.cds_exon_lengths_fp as cds_exon_lengths_fp_eq + FROM tx_def_summary_mv D1 + JOIN tx_def_summary_mv D2 on (D1.tx_ac != D2.tx_ac + and (D1.hgnc=D2.hgnc + or D1.cds_md5=D2.cds_md5 + or D1.es_fingerprint=D2.es_fingerprint + or D1.cds_es_fp=D2.cds_es_fp + or D1.cds_exon_lengths_fp=D2.cds_exon_lengths_fp + )); + """) + # ### end of updates to views ### + + # ### commands auto generated by Alembic - please adjust! ### + op.drop_constraint("fk_uta_transcript_gene_gene_id", "transcript", schema="uta", type_="foreignkey") + op.drop_index(op.f("ix_uta_transcript_gene_id"), table_name="transcript", schema="uta") + op.alter_column("transcript", "gene_id", + existing_type=sa.TEXT(), + nullable=True, + schema="uta") + op.drop_index(op.f("ix_uta_gene_hgnc"), table_name="gene", schema="uta") + op.drop_constraint("gene_pkey", "gene", schema="uta") + op.alter_column("gene", "gene_id", + existing_type=sa.TEXT(), + nullable=True, + schema="uta") + op.drop_index(op.f("ix_uta_gene_symbol"), table_name="gene", schema="uta") + op.drop_column("gene", "symbol", schema="uta") + # ### end Alembic commands ### diff --git a/src/uta/loading.py b/src/uta/loading.py index 3939cbc..9837f99 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -336,40 +336,17 @@ def load_geneinfo(session, opts, cf): for i_gi, gi in enumerate(gir): session.merge( usam.Gene( + gene_id=gi.gene_id, hgnc=gi.hgnc, + symbol=gi.gene_symbol, maploc=gi.maploc, descr=gi.descr, summary=gi.summary, aliases=gi.aliases, + type=gi.type, + xrefs=gi.xrefs, )) - logger.info("Added {gi.hgnc} ({gi.summary})".format(gi=gi)) - session.commit() - - -def load_ncbi_geneinfo(session, opts, cf): - """ - import data as downloaded (by you) from - ftp://ftp.ncbi.nlm.nih.gov/gene/DATA/gene_info.gz - """ - - session.execute(text("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role")))) - session.execute(text("set search_path = " + usam.schema_name)) - - gip = uta.parsers.geneinfo.GeneInfoParser(gzip.open(opts["FILE"], 'rt')) - for gi in gip: - if gi["tax_id"] != "9606" or gi["Symbol_from_nomenclature_authority"] == "-": - continue - g = usam.Gene( - gene_id=gi["GeneID"], - hgnc=gi["Symbol_from_nomenclature_authority"], - maploc=gi["map_location"], - descr=gi["Full_name_from_nomenclature_authority"], - aliases=gi["Synonyms"], - strand=gi[""], - ) - session.add(g) - logger.info("loaded gene {g.hgnc} ({g.descr})".format(g=g)) + logger.info("Added {gi.gene_symbol}: {gi.gene_id} ({gi.summary})".format(gi=gi)) session.commit() @@ -698,16 +675,15 @@ def _fetch_origin_by_name(name): u_tx = usam.Transcript( ac=ti.ac, origin=ori, - hgnc=ti.gene_symbol, + gene_id=ti.gene_id, cds_start_i=cds_start_i, cds_end_i=cds_end_i, cds_md5=cds_md5, ) session.add(u_tx) - if u_tx.hgnc != ti.gene_symbol: - logger.warn("{ti.ac}: HGNC symbol changed from {u_tx.hgnc} to {ti.gene_symbol}".format( + if u_tx.gene_id != ti.gene_id: + raise Exception("{ti.ac}: GeneID changed from {u_tx.gene_id} to {ti.gene_id}".format( u_tx=u_tx, ti=ti)) - u_tx.hgnc = ti.gene_symbol # state: transcript now exists, either existing or freshly-created diff --git a/src/uta/models.py b/src/uta/models.py index 31a5392..58476d9 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -98,13 +98,17 @@ class Gene(Base): __tablename__ = "gene" # columns: - hgnc = sa.Column(sa.Text, primary_key=True) + gene_id = sa.Column(sa.Text, primary_key=True) + hgnc = sa.Column(sa.Text, nullable=False, index=True) + symbol = sa.Column(sa.Text, nullable=False, index=True) maploc = sa.Column(sa.Text) descr = sa.Column(sa.Text) summary = sa.Column(sa.Text) aliases = sa.Column(sa.Text) added = sa.Column( sa.DateTime, nullable=False, default=datetime.datetime.now()) + type = sa.Column(sa.Text) + xrefs = sa.Column(sa.Text) # methods: @@ -124,9 +128,9 @@ class Transcript(Base): ac = sa.Column(sa.Text, primary_key=True) origin_id = sa.Column( sa.Integer, sa.ForeignKey("origin.origin_id", onupdate="CASCADE", ondelete="CASCADE"), nullable=False, index=True) - hgnc = sa.Column(sa.Text) # , sa.ForeignKey("gene.hgnc")) - cds_start_i = sa.Column(sa.Integer) #, nullable=False) - cds_end_i = sa.Column(sa.Integer) #, nullable=False) + gene_id = sa.Column(sa.Text, sa.ForeignKey("gene.gene_id"), nullable=False, index=True) + cds_start_i = sa.Column(sa.Integer) + cds_end_i = sa.Column(sa.Integer) cds_md5 = sa.Column(sa.Text, index=True) added = sa.Column( sa.DateTime, default=datetime.datetime.now(), nullable=False) diff --git a/tests/test_uta_loading.py b/tests/test_uta_loading.py index 1fbd029..711d714 100644 --- a/tests/test_uta_loading.py +++ b/tests/test_uta_loading.py @@ -48,11 +48,37 @@ def test_load_assoc_ac(self): ) self.session.add(o1) + # insert genes required for transcripts + g1 = usam.Gene( + gene_id='49', + hgnc='ACR', + symbol='ACR', + maploc='22q13.33', + descr='acrosin', + summary='acrosin', + aliases='SPGF87', + type='protein-coding', + xrefs='MIM:102480,HGNC:HGNC:126,Ensembl:ENSG00000100312,AllianceGenome:HGNC:126', + ) + g2 = usam.Gene( + gene_id=50, + hgnc='ACO2', + symbol='ACO2', + maploc='22q13.2', + descr='aconitase 2', + summary='aconitase 2', + aliases='ACONM,HEL-S-284,ICRD,OCA8,OPA9', + type='protein-coding', + xrefs='MIM:100850,HGNC:HGNC:118,Ensembl:ENSG00000100412,AllianceGenome:HGNC:118', + ) + self.session.add(g1) + self.session.add(g2) + # insert transcripts referenced in data file t1 = usam.Transcript( ac='NM_001097.3', origin=o1, - hgnc='ACR', + gene_id=g1.gene_id, cds_start_i=0, cds_end_i=1, cds_md5='a', @@ -60,7 +86,7 @@ def test_load_assoc_ac(self): t2 = usam.Transcript( ac='NM_001098.3', origin=o1, - hgnc='ACO2', + gene_id=g2.gene_id, cds_start_i=2, cds_end_i=3, cds_md5='b', diff --git a/tests/test_uta_models.py b/tests/test_uta_models.py index 3d81cb4..d221d16 100644 --- a/tests/test_uta_models.py +++ b/tests/test_uta_models.py @@ -77,7 +77,9 @@ def setUpClass(cls): cls.session.add(o) g = usam.Gene( + gene_id='148', hgnc='ADRA1A', + symbol='ADRA1A', maploc='8p21.2', descr='adrenoceptor alpha 1A', summary='''Alpha-1-adrenergic receptors (alpha-1-ARs) are @@ -121,7 +123,7 @@ def setUpClass(cls): t = usam.Transcript( ac=ac, origin=o, - hgnc=g.hgnc, + gene_id=g.gene_id, cds_start_i=tx_info['t_cds_start_i'], cds_end_i=tx_info['t_cds_end_i'], cds_md5='d41d8cd98f00b204e9800998ecf8427e', From c2af389a8b390ed6ea43307cf5950602a983a0f3 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Wed, 24 Apr 2024 12:16:43 -0600 Subject: [PATCH 35/54] IPVC-2379: add necessary NCBI input files to download config (#25) --- docker-compose.yml | 2 +- etc/ncbi-files.txt | 57 ++++++++++++++++++++++++++++++++++++++++++++++ sbin/ncbi-download | 38 +++++-------------------------- 3 files changed, 64 insertions(+), 33 deletions(-) create mode 100644 etc/ncbi-files.txt diff --git a/docker-compose.yml b/docker-compose.yml index b689645..cad902f 100644 --- a/docker-compose.yml +++ b/docker-compose.yml @@ -5,7 +5,7 @@ version: '3' services: ncbi-download: image: uta-update - command: sbin/ncbi-download /ncbi-dir + command: sbin/ncbi-download etc/ncbi-files.txt /ncbi-dir volumes: - .:/opt/repos/uta - ${UTA_ETL_NCBI_DIR}:/ncbi-dir diff --git a/etc/ncbi-files.txt b/etc/ncbi-files.txt new file mode 100644 index 0000000..6cb31ef --- /dev/null +++ b/etc/ncbi-files.txt @@ -0,0 +1,57 @@ +# This configuration file contains the paths to the NCBI data files needed by the SeqRepo/UTA load pipelines. +# +# ├── gene +# │ └── DATA +# │ ├── GENE_INFO +# │ │ └── Mammalia +# │ │ └── Homo_sapiens.gene_info.gz +# │ └── gene2accession.gz +# ├── genomes +# │ └── refseq +# │ └── vertebrate_mammalian +# │ └── Homo_sapiens +# │ └── all_assembly_versions +# │ └── GCF_000001405.25_GRCh37.p13 +# │ ├── GCF_000001405.25_GRCh37.p13_genomic.fna.gz +# │ └── GCF_000001405.25_GRCh37.p13_genomic.gff.gz +# └── refseq +# └── H_sapiens +# ├── historical +# │ └── GRCh38 +# │ └── GCF_000001405.40-RS_2023_03_historical +# │ ├── GCF_000001405.40-RS_2023_03_knownrefseq_alns.gff.gz +# │ └── GCF_000001405.40-RS_2023_03_knownrefseq_rna.gbff.gz +# └── mRNA_Prot +# ├── human.1.protein.faa.gz +# ├── human.1.rna.fna.gz +# ├── human.1.rna.gbff.gz +# ├── ... + +## Gene Data +gene/DATA/gene2refseq.gz +gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz + +## RefSeq Data +refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz +refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz +refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz + +## Historical RefSeq alignments +refseq/H_sapiens/historical/GRCh38/GCF_000001405.40-RS_2023_03_historical/GCF_000001405.40-RS_2023_03_knownrefseq_alns.gff.gz +refseq/H_sapiens/historical/GRCh38/GCF_000001405.40-RS_2023_03_historical/GCF_000001405.40-RS_2023_03_knownrefseq_rna.gbff.gz + +## Genome build and alignment data +# Build 37 +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.25_GRCh37.p13/GCF_000001405.25_GRCh37.p13_assembly_report.txt +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.25_GRCh37.p13/GCF_000001405.25_GRCh37.p13_genomic.fna.gz +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.25_GRCh37.p13/GCF_000001405.25_GRCh37.p13_genomic.gff.gz + +# Build 38 +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.40_GRCh38.p14/GCF_000001405.40_GRCh38.p14_assembly_report.txt +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.40_GRCh38.p14/GCF_000001405.40_GRCh38.p14_genomic.fna.gz +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.40_GRCh38.p14/GCF_000001405.40_GRCh38.p14_genomic.gff.gz + +# T2Tv2.0 +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_assembly_report.txt +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.fna.gz +genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz diff --git a/sbin/ncbi-download b/sbin/ncbi-download index dc445cd..09ad4c2 100755 --- a/sbin/ncbi-download +++ b/sbin/ncbi-download @@ -2,48 +2,22 @@ # This script downloads the files needed for a UTA+SeqRepo update into to the given directory. # -# DONWLOAD_DIR will have the following structure: -# -# ├── gene -# │ └── DATA -# │ ├── GENE_INFO -# │ │ └── Mammalia -# │ │ └── Homo_sapiens.gene_info.gz -# │ └── gene2accession.gz -# ├── genomes -# │ └── refseq -# │ └── vertebrate_mammalian -# │ └── Homo_sapiens -# │ └── all_assembly_versions -# │ └── GCF_000001405.25_GRCh37.p13 -# │ ├── GCF_000001405.25_GRCh37.p13_genomic.fna.gz -# │ └── GCF_000001405.25_GRCh37.p13_genomic.gff.gz -# └── refseq -# └── H_sapiens -# └── mRNA_Prot -# ├── human.1.protein.faa.gz -# ├── human.1.rna.fna.gz -# └── human.1.rna.gbff.gz +# DESTINATION_DIR will have a directory structure matching the source. set -e -DOWNLOAD_DIR=$1 -DOWNLOAD_PATHS=( - 'gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gene_info.gz' - 'gene/DATA/gene2accession.gz' - 'refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz' -) +FILE_PATH_CONFIG=$1 +DOWNLOAD_DIR=$2 -if [ -z "$DOWNLOAD_DIR" ] +if [ -z "$FILE_PATH_CONFIG" ] || [ -z "$DOWNLOAD_DIR" ] then - echo 'Usage: sbin/ncbi-download ' + echo 'Usage: sbin/ncbi-download ' exit 1 else echo "Downloading files to $DOWNLOAD_DIR" fi -for DOWNLOAD_PATH in "${DOWNLOAD_PATHS[@]}" -do +grep -v -e '^#' -e '^$' "$FILE_PATH_CONFIG" | while read -r DOWNLOAD_PATH; do # each top-level directory in NCBI is an rsync module. # bash parameter expansion removes all content after first slash. 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zY}b{N1tzavxF*$abv;%n~G{e2tq8!v9{pc)a9p|Sb4SGwr}&lBS` ztpdD=v26RD5cCyoH+ySqIT;HLr41yf7F2m_7-dw{+TFGI&WLLnL+a>GB~P8v8ayBc|h*{{kD)lBWOw literal 0 HcmV?d00001 From 8ff7464f3dc88c7861c859f9264ceee6e73788cd Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Thu, 25 Apr 2024 16:00:30 -0600 Subject: [PATCH 37/54] IPVC-2344: Update SeqRepo load to discover local fasta files (#26) --- Dockerfile | 2 +- docker-compose.yml | 4 ++-- sbin/seqrepo-load | 4 ++-- sbin/uta-extract | 4 +--- 4 files changed, 6 insertions(+), 8 deletions(-) diff --git a/Dockerfile b/Dockerfile index e58df99..4b83e36 100644 --- a/Dockerfile +++ b/Dockerfile @@ -4,7 +4,7 @@ FROM ubuntu:22.04 as uta ARG python_version="3.10" # list and install dependencies -ARG dependencies="python${python_version} python3-dev python3-pip rsync git postgresql-client-14" +ARG dependencies="python${python_version} python3-dev python3-pip rsync git postgresql-client-14 tabix" RUN apt-get update && apt-get install -y $dependencies && apt-get clean diff --git a/docker-compose.yml b/docker-compose.yml index cad902f..c7c9f6e 100644 --- a/docker-compose.yml +++ b/docker-compose.yml @@ -23,7 +23,7 @@ services: network_mode: host seqrepo-load: image: uta-update - command: sbin/seqrepo-load /usr/local/share/seqrepo 2024-02-20 /seqrepo-load/work /seqrepo-load/logs + command: sbin/seqrepo-load /usr/local/share/seqrepo ${UTA_ETL_OLD_SEQREPO_VERSION} /seqrepo-load/work /seqrepo-load/logs volumes: - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo - ${UTA_ETL_WORK_DIR}:/seqrepo-load/work @@ -62,7 +62,7 @@ services: network_mode: host splign-manual: image: uta-update - command: sbin/uta-splign-manual 2024-02-20 ${UTA_ETL_OLD_UTA_VERSION} /uta-splign-manual/input /uta-splign-manual/work /uta-splign-manual/logs + command: sbin/uta-splign-manual ${UTA_ETL_OLD_SEQREPO_VERSION} ${UTA_ETL_OLD_UTA_VERSION} /uta-splign-manual/input /uta-splign-manual/work /uta-splign-manual/logs depends_on: uta: condition: service_healthy diff --git a/sbin/seqrepo-load b/sbin/seqrepo-load index 93942cf..adadc04 100755 --- a/sbin/seqrepo-load +++ b/sbin/seqrepo-load @@ -16,6 +16,6 @@ fi ## Load SeqRepo with new sequences seqrepo --root-directory "$seqrepo_root" \ load -n NCBI --instance-name "$seqrepo_version" \ - $sequence_dir/*.fna.gz \ - $sequence_dir/*.faa.gz 2>& 1 | \ + "$sequence_dir"/*.fna.gz \ + "$sequence_dir"/*.faa.gz 2>& 1 | \ tee "$log_dir/seqrepo-load.log" diff --git a/sbin/uta-extract b/sbin/uta-extract index a8d3d60..c4e239d 100755 --- a/sbin/uta-extract +++ b/sbin/uta-extract @@ -38,6 +38,4 @@ sbin/filter_exonset_transcripts.py --tx-info "$working_dir/txinfo.gz" --exonsets tee "$log_dir/filter_exonset_transcripts.log" # move fasta files into same dir -cp -f $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz $working_dir/ -cp -f $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz $working_dir/ -cp -f $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.fna.gz $working_dir/ +find "$ncbi_dir" -type f -name "*.f[an]a.gz" -print0 | xargs -i --null cp {} "$working_dir/" From b915f31c158de3f49a6d332e3ee8dc76d4d0bdad Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Fri, 26 Apr 2024 12:14:41 -0600 Subject: [PATCH 38/54] =?UTF-8?q?fix(IPVC-2399):=20fix=20gene=20id=20back?= =?UTF-8?q?=20fill=20migration=20script,=20update=20paths=20=E2=80=A6=20(#?= =?UTF-8?q?29)?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- misc/gene-update/backfill_gene_id.py | 5 +++-- misc/gene-update/upgrade-uta-schema.sh | 4 ++-- ...set_gene_id_and_primary_and_foreign_keys.py | 18 ++++++------------ 3 files changed, 11 insertions(+), 16 deletions(-) diff --git a/misc/gene-update/backfill_gene_id.py b/misc/gene-update/backfill_gene_id.py index 5961cfd..350ef46 100644 --- a/misc/gene-update/backfill_gene_id.py +++ b/misc/gene-update/backfill_gene_id.py @@ -7,6 +7,7 @@ import uta from uta.models import Gene, Transcript +from uta.tools.file_utils import open_file logger = None @@ -22,7 +23,7 @@ def backfill_gene(uta_session: Session, gene_update_file: str) -> None: now_ts = datetime.now() i = 0 new_genes = [] - with open(gene_update_file) as f: + with open_file(gene_update_file) as f: for line in f: if line.startswith("gene_id"): continue @@ -70,7 +71,7 @@ def backfill_transcript(uta_session: Session, transcript_update_file: str) -> No tx_ac_to_gene_id = {} logger.info(f"Reading transcript to gene id mappings from {transcript_update_file}") - with open(transcript_update_file) as f: + with open_file(transcript_update_file) as f: for line in f: if line.startswith("origin"): continue diff --git a/misc/gene-update/upgrade-uta-schema.sh b/misc/gene-update/upgrade-uta-schema.sh index 0671780..dab0ef0 100644 --- a/misc/gene-update/upgrade-uta-schema.sh +++ b/misc/gene-update/upgrade-uta-schema.sh @@ -44,8 +44,8 @@ alembic -c etc/alembic.ini upgrade 595a586e6de7 # run data migration to back fill gene_id python misc/gene-update/backfill_gene_id.py \ postgresql://uta_admin:@localhost/uta \ - /workdir/backfill/gene_update.tsv \ - /workdir/backfill/transcript_update.tsv + misc/gene-update/gene_update.tsv.gz \ + misc/gene-update/transcript_update.tsv.gz # run Alembic migrations to add constraints and update existing views alembic -c etc/alembic.ini upgrade head diff --git a/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py b/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py index 094d05a..62cabe1 100644 --- a/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py +++ b/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py @@ -35,23 +35,17 @@ def upgrade() -> None: unique=False, schema="uta", ) - op.create_foreign_key( - "fk_uta_transcript_gene_gene_id", - "transcript", - "gene", - ["gene_id"], - ["gene_id"], - source_schema="uta", - referent_schema="uta", - onupdate="RESTRICT", - ondelete="RESTRICT", - ) + op.create_foreign_key(None, 'transcript', 'gene', ['gene_id'], ['gene_id'], source_schema='uta', referent_schema='uta') # ### end Alembic commands ### # ### handle first part of hgnc -> gene_symbol column rename ### op.add_column("gene", sa.Column("symbol", sa.Text(), nullable=True), schema="uta") op.create_index(op.f("ix_uta_gene_symbol"), "gene", ["symbol"], unique=False, schema="uta") op.execute("UPDATE gene SET symbol = hgnc;") + op.alter_column('gene', 'symbol', + existing_type=sa.TEXT(), + nullable=False, + schema='uta') # ### end of hgnc -> gene_symbol column rename ### # ### updates required to existing views needed to drop hgnc from transcript. ### @@ -301,7 +295,7 @@ def downgrade() -> None: # ### end of updates to views ### # ### commands auto generated by Alembic - please adjust! ### - op.drop_constraint("fk_uta_transcript_gene_gene_id", "transcript", schema="uta", type_="foreignkey") + op.drop_constraint(None, 'transcript', schema='uta', type_='foreignkey') op.drop_index(op.f("ix_uta_transcript_gene_id"), table_name="transcript", schema="uta") op.alter_column("transcript", "gene_id", existing_type=sa.TEXT(), From c007662b9c741a2111a9962a8d59514d24ca665d Mon Sep 17 00:00:00 2001 From: nvta1209 <162694616+nvta1209@users.noreply.github.com> Date: Mon, 29 Apr 2024 11:16:50 -0700 Subject: [PATCH 39/54] feat(IPVC-2390): translation exceptions (#28) --- sbin/generate-loading-data | 6 +- sbin/ncbi-parse-gbff | 18 ++-- sbin/ncbi_process_mito.py | 20 ++-- ...f90d_create_translation_exception_table.py | 37 +++++++ src/uta/formats/txinfo.py | 23 ++++- src/uta/loading.py | 48 ++++++++- src/uta/models.py | 24 +++++ src/uta/parsers/seqrecord.py | 22 +++- tests/data/txinfo.gz | Bin 0 -> 164 bytes tests/test_ncbi_process_mito.py | 2 +- tests/test_uta_formats_txinfo.py | 10 ++ tests/test_uta_loading.py | 95 ++++++++++++++++++ tests/test_uta_models.py | 38 +++++++ tests/test_uta_parsers_seqrecord.py | 31 +++++- 14 files changed, 341 insertions(+), 33 deletions(-) create mode 100644 src/alembic/versions/14eed54ff90d_create_translation_exception_table.py create mode 100644 tests/data/txinfo.gz create mode 100644 tests/test_uta_formats_txinfo.py diff --git a/sbin/generate-loading-data b/sbin/generate-loading-data index e02d3be..0b2fad3 100755 --- a/sbin/generate-loading-data +++ b/sbin/generate-loading-data @@ -96,7 +96,9 @@ def parse_splign(fn, txdata): gene_id=gene_id, gene_symbol=gene_symbol, cds_se_i=cds_se_i, - exons_se_i=tx_exons_str) + exons_se_i=tx_exons_str, + transl_except=None, + ) exonset = uta.formats.exonset.ExonSet( tx_ac=tx_ac, alt_ac=alt_ac, @@ -116,7 +118,7 @@ if __name__ == "__main__": txinfo_out = uta.formats.txinfo.TxInfoWriter(gzip.open(os.path.join(opts.output_dir, txinfo_fn), "wt")) exonset_out = uta.formats.exonset.ExonSetWriter(gzip.open(os.path.join(opts.output_dir, exonset_fn), "wt")) - + for fn in opts.FILES: _logger.info("# " + fn) txinfo, exonset = parse_splign(fn, txdata) diff --git a/sbin/ncbi-parse-gbff b/sbin/ncbi-parse-gbff index ba76e2b..3b3b7ff 100755 --- a/sbin/ncbi-parse-gbff +++ b/sbin/ncbi-parse-gbff @@ -113,15 +113,15 @@ if __name__ == "__main__": skipped_ids.add(srf.id) continue cds_se_i = srf.cds_se_i - ti = TxInfo(ac=srf.id, - origin=opts.origin, - gene_id=srf.gene_id, - gene_symbol=srf.gene_symbol, - cds_se_i=None if cds_se_i is None else "{},{}".format( - *cds_se_i), - exons_se_i=";".join( - ["{},{}".format(*ese) for ese in srf.exons_se_i]) - ) + ti = TxInfo( + ac=srf.id, + origin=opts.origin, + gene_id=srf.gene_id, + gene_symbol=srf.gene_symbol, + cds_se_i=None if cds_se_i is None else "{},{}".format(*cds_se_i), + exons_se_i=";".join(["{},{}".format(*ese) for ese in srf.exons_se_i]), + transl_except=TxInfo.serialize_transl_except(srf.transl_except), + ) tiw.write(ti) genes.add(srf.gene_symbol) logger.info("{ng} genes in {fn} ({c})".format(ng=len(genes), fn=fn, c=prefixes)) diff --git a/sbin/ncbi_process_mito.py b/sbin/ncbi_process_mito.py index c65984b..ccd1d69 100755 --- a/sbin/ncbi_process_mito.py +++ b/sbin/ncbi_process_mito.py @@ -58,14 +58,14 @@ import importlib_resources import logging import logging.config -from typing import Dict, Optional +from typing import Dict, List, Optional from Bio.Seq import Seq import Bio.SeqIO from Bio.SeqFeature import SeqFeature from Bio.SeqRecord import SeqRecord from bioutils.digests import seq_md5 -from more_itertools import first, one +from more_itertools import one from uta.formats.geneaccessions import GeneAccessions, GeneAccessionsWriter from uta.formats.seqinfo import SeqInfo, SeqInfoWriter @@ -90,7 +90,7 @@ class MitoGeneData: origin: str = "NCBI" alignment_method: str = "splign" transl_table: Optional[str] = None - transl_except: Optional[str] = None + transl_except: Optional[List[str]] = None pro_ac: Optional[str] = None pro_seq: Optional[str] = None @@ -195,11 +195,6 @@ def get_mito_genes(gbff_filepath: str): assert int(xrefs["GeneID"]) == gene_id assert feature_start == feature.location.start assert feature_end == feature.location.end - # if feature type not CDS, set defaults - pro_ac = None - pro_seq = None - transl_table = None - transl_except = None # retrieve sequence, and reverse compliment if on reverse strand ac = f"{record.id}_{feature.location.start:05}_{feature.location.end:05}" @@ -212,8 +207,12 @@ def get_mito_genes(gbff_filepath: str): pro_ac = one(feature.qualifiers["protein_id"]) pro_seq = str(one(feature.qualifiers["translation"])) transl_table = one(feature.qualifiers["transl_table"]) - if "transl_except" in feature.qualifiers: - transl_except = one(feature.qualifiers["transl_except"]) + transl_except = feature.qualifiers.get("transl_except") + else: + pro_ac = None + pro_seq = None + transl_table = None + transl_except = None # yield gene data yield MitoGeneData( @@ -313,6 +312,7 @@ def main(ncbi_accession: str, output_dir: str): mg.gene_symbol, mg.cds_se_i(), mg.exons_se_i(), + TxInfo.serialize_transl_except(mg.transl_except), ) ) diff --git a/src/alembic/versions/14eed54ff90d_create_translation_exception_table.py b/src/alembic/versions/14eed54ff90d_create_translation_exception_table.py new file mode 100644 index 0000000..b55a336 --- /dev/null +++ b/src/alembic/versions/14eed54ff90d_create_translation_exception_table.py @@ -0,0 +1,37 @@ +"""create translation_exception table + +Revision ID: 14eed54ff90d +Revises: f85dd97bd9f5 +Create Date: 2024-04-25 23:57:12.455316 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa + + +# revision identifiers, used by Alembic. +revision: str = '14eed54ff90d' +down_revision: Union[str, None] = 'f85dd97bd9f5' +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + op.create_table( + 'translation_exception', + sa.Column('translation_exception_id', sa.Integer(), autoincrement=True, nullable=False), + sa.Column('tx_ac', sa.Text(), nullable=False), + sa.Column('start_position', sa.Integer(), nullable=False), + sa.Column('end_position', sa.Integer(), nullable=False), + sa.Column('amino_acid', sa.Text(), nullable=False), + sa.CheckConstraint('start_position <= end_position', name='start_less_than_or_equal_to_end'), + sa.ForeignKeyConstraint(['tx_ac'], ['uta.transcript.ac'], onupdate='CASCADE', ondelete='CASCADE'), + sa.PrimaryKeyConstraint('translation_exception_id'), + schema='uta', + ) + + +def downgrade() -> None: + op.drop_table('translation_exception', schema='uta') diff --git a/src/uta/formats/txinfo.py b/src/uta/formats/txinfo.py index fa811fd..c869c42 100644 --- a/src/uta/formats/txinfo.py +++ b/src/uta/formats/txinfo.py @@ -1,10 +1,23 @@ import csv import recordtype - - -class TxInfo(recordtype.recordtype('TxInfo', - ['origin', 'ac', 'gene_id', 'gene_symbol', 'cds_se_i', 'exons_se_i'])): - pass +from typing import List, Optional + + +# transl_except should be a semicolon-separated list: +# (pos:333..335,aa:Sec);(pos:1017,aa:TERM) +class TxInfo( + recordtype.recordtype( + 'TxInfo', + ['origin', 'ac', 'gene_id', 'gene_symbol', 'cds_se_i', 'exons_se_i', 'transl_except'], +)): + + @staticmethod + def serialize_transl_except(transl_except_list: Optional[List[str]]) -> Optional[str]: + """Helper for formatting transl_except list as a string.""" + if transl_except_list is None: + return None + else: + return ";".join(transl_except_list) class TxInfoWriter(csv.DictWriter): diff --git a/src/uta/loading.py b/src/uta/loading.py index 9837f99..90cdc2f 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -7,7 +7,7 @@ import itertools import logging import time -from typing import Any +from typing import Any, Dict, List from biocommons.seqrepo import SeqRepo from bioutils.coordinates import strand_pm_to_int, MINUS_STRAND @@ -681,9 +681,17 @@ def _fetch_origin_by_name(name): cds_md5=cds_md5, ) session.add(u_tx) + + if ti.transl_except: + # if transl_except exists, it looks like this: + # (pos:333..335,aa:Sec);(pos:1017,aa:TERM) + transl_except_list = ti.transl_except.split(';') + te_list = _create_translation_exceptions(transcript=ti.ac, transl_except_list=transl_except_list) + for te in te_list: + session.add(usam.TranslationException(**te)) + if u_tx.gene_id != ti.gene_id: - raise Exception("{ti.ac}: GeneID changed from {u_tx.gene_id} to {ti.gene_id}".format( - u_tx=u_tx, ti=ti)) + raise Exception("{ti.ac}: GeneID changed from {u_tx.gene_id} to {ti.gene_id}".format(u_tx=u_tx, ti=ti)) # state: transcript now exists, either existing or freshly-created @@ -709,6 +717,40 @@ def _fetch_origin_by_name(name): p=(i_ti + 1) / n_rows * 100)) +def _create_translation_exceptions(transcript: str, transl_except_list: List[str]) -> List[Dict]: + """ + Create TranslationException object data where start and end positions are 0-based, from transl_except data that is 1-based. + For example, [(pos:333..335,aa:Sec), (pos:1017,aa:TERM)] should result in start and end positions [(332, 335), (1016, 1017)] + """ + result = [] + + for te in transl_except_list: + # remove parens + te = te.replace('(','').replace(')','') + + # extract positions + pos_str, aa_str = te.split(',') + pos_str = pos_str.removeprefix('pos:') + if '..' in pos_str: + start_position, _, end_position = pos_str.partition('..') + else: + start_position = end_position = pos_str + + # extract amino acid + amino_acid = aa_str.removeprefix('aa:') + + result.append( + { + 'tx_ac': transcript, + 'start_position': int(start_position) - 1, + 'end_position': int(end_position), + 'amino_acid': amino_acid, + } + ) + + return result + + def refresh_matviews(session, opts, cf): session.execute(text("set role {admin_role};".format( admin_role=cf.get("uta", "admin_role")))) diff --git a/src/uta/models.py b/src/uta/models.py index 58476d9..3f3076a 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -140,6 +140,30 @@ class Transcript(Base): origin = sao.relationship("Origin", backref="transcripts") +class TranslationException(Base): + """ + Represents `transl_except` annotations on CDS features in transcript records from NCBI. + + Examples: + /transl_except=(pos:333..335,aa:Sec) + /transl_except=(pos:1017,aa:TERM) + """ + + __tablename__ = "translation_exception" + __table_args__ = ( + sa.CheckConstraint("start_position <= end_position", "start_less_than_or_equal_to_end"), + ) + + translation_exception_id = sa.Column(sa.Integer, autoincrement=True, primary_key=True) + tx_ac = sa.Column(sa.Text, sa.ForeignKey("transcript.ac", onupdate="CASCADE", ondelete="CASCADE"), nullable=False) + start_position = sa.Column(sa.Integer, nullable=False) + end_position = sa.Column(sa.Integer, nullable=False) + amino_acid = sa.Column(sa.Text, nullable=False) + + # relationships: + transcript = sao.relationship("Transcript", backref="translation_exceptions") + + class ExonSet(Base): __tablename__ = "exon_set" __table_args__ = ( diff --git a/src/uta/parsers/seqrecord.py b/src/uta/parsers/seqrecord.py index f8bf57e..3497f4d 100644 --- a/src/uta/parsers/seqrecord.py +++ b/src/uta/parsers/seqrecord.py @@ -1,6 +1,6 @@ from collections import defaultdict from functools import cached_property -from typing import Union +from typing import List, Optional import Bio.SeqRecord from Bio.SeqFeature import SeqFeature @@ -31,9 +31,20 @@ def validate_features_by_type(features: dict[str, list]) -> None: if "gene" not in features or len(features["gene"]) != 1: raise SeqRecordFeatureError("Expected exactly one `gene` feature") + @cached_property + def cds_feature(self) -> Optional[SeqFeature]: + """ + Returns the CDS feature for any coding transcript, None for any non-coding transcript. + """ + cds_features = self.features_by_type.get("CDS") + if cds_features is None: + return None + else: + return cds_features[0] + @cached_property def gene_feature(self) -> SeqFeature: - """Returns the gene feature, which is assumed to exist for all transcripts. """ + """Returns the gene feature, which should exist for all transcripts.""" return self.features_by_type.get("gene")[0] @property @@ -67,3 +78,10 @@ def exons_se_i(self): else: exons = [self.gene_feature] return [(f.location.start.real, f.location.end.real) for f in exons] + + @property + def transl_except(self) -> Optional[List[str]]: + if self.cds_feature is None: + return None + else: + return self.cds_feature.qualifiers.get("transl_except") diff --git a/tests/data/txinfo.gz b/tests/data/txinfo.gz new file mode 100644 index 0000000000000000000000000000000000000000..a2f4776388f3de042dd9f763c3b5281ff13f4a93 GIT binary patch literal 164 zcmV;V09*ebiwFo0-YaGR19W(4Zf0))EyzI%gD?<<;q`cu7J`hEn5x;exF}S+@Bkr= zK?IwKaba)YK)d+(A1`e4GCg+`=$6w>;{R#`%G3uCJa7Xa}xo^hDMZ3egb Date: Fri, 3 May 2024 08:05:13 -0600 Subject: [PATCH 40/54] feat(IPVC-2195): add script to generate alignment metrics (#30) --- misc/generate_alignment_metrics.py | 301 +++++++++++++++++++++++++++++ 1 file changed, 301 insertions(+) create mode 100644 misc/generate_alignment_metrics.py diff --git a/misc/generate_alignment_metrics.py b/misc/generate_alignment_metrics.py new file mode 100644 index 0000000..0e51c8f --- /dev/null +++ b/misc/generate_alignment_metrics.py @@ -0,0 +1,301 @@ +""" +To determine the quality of an alignment in UTA this script will compute metrics that can be used to evaluate +the quality of the alignment. + +The metrics are: + seq_length: max(exon.ends_i) + exon_count: count of exons linked to transcript (from genbank file) + aligned_exon_count: count of blocks from GFF file + exon_structure_mismatch: True if exon_count != aligned_exon_count + matches: count of matching bases between chromosome and transcript within alignment bounds + mismatches: count of mismatched bases between chromosome and transcript within alignment bounds + gap_count: count of gaps (indels) between chromosome and transcript sequenceswithin alignment bounds + aln_length: total number of alignment blocks, includes counts of indel positions + identity_gapped: matches / aln_length ## not gap compressed identity calculation + identity_ungapped: matches / (matches + mismatches) + coverage: (matches + mismatches + deletions) / seq_length + +Example: + + 1 11 21 31 41 + Chromo+: 1 CCAGTGTGGC CGATACCCCA GGTTGGC-AC GCATCGTTGC CTTGGTAAGC 49 + |||||||||| |||| ||| || || || |||||||||| |||||||||| + Refseq+: 1 CCAGTGTGGC CGATGCCC-- -GT--GCTAC GCATCGTTGC CTTGGTAAGC 45 + + seq_length: 45 + exon_count: 1 + aligned_exon_count: 1 + matches: 43 + mismatches: 1 + gap_count: 3 + aln_length: 43 matches + 1 mismatch + 3bp insertion + 2bp insertion + 1bp deletion = 50 + identity_gapped: 43 / 50 = 0.86 + identity_ungapped: 43 / (43 + 1) = 0.9772 + coverage: (43 + 1 + 1) / 45 = 1.0 + +Usage: + python generate_alignment_metrics.py --db-url --schema-name +""" + +import argparse +import logging +import re +from dataclasses import dataclass, field + +import psycopg2 +import six + +import uta + + +@dataclass +class CigarAln: + op: str + length: int + + +@dataclass +class ExonAln: + tx_start_i: int + tx_end_i: int + alt_start_i: int + alt_end_i: int + cigar: str + cigar_alns: list[CigarAln] = field(default_factory=list) + + +@dataclass +class TxAln: + hgnc: str + tx_ac: str + seq_length: int + exon_count: int + alt_ac: str + alt_aln_method: str + alt_strand: int + aligned_exon_count: int + exon_alignments: list[ExonAln] = field(default_factory=list) + + @staticmethod + def metrics_header(): + return "{}\n".format("\t".join([ + "hgnc", + "tx_ac", + "seq_length", + "exon_count", + "alt_ac", + "alt_aln_method", + "alt_strand", + "aligned_exon_count", + "exon_structure_mismatch", + "matches_bps", + "mismatches_bps", + "gap_count", + "deletions_bps", + "aln_length", + "identity_gap", + "identity_ungap", + "coverage", + ])) + + def to_metric_output_row(self): + return "{}\n".format("\t".join(map(str, [ + self.hgnc, + self.tx_ac, + self.seq_length, + self.exon_count, + self.alt_ac, + self.alt_aln_method, + self.alt_strand, + self.aligned_exon_count, + not self.exon_count == self.aligned_exon_count, + self.matches(), + self.mismatches(), + self.gap_count(), + self.deletions(), + self.aln_length(), + self.identity_gap(), + self.identity_ungap(), + self.coverage(), + ]))) + + def matches(self): + matches = 0 + for exon_aln in self.exon_alignments: + for cigar_aln in exon_aln.cigar_alns: + if cigar_aln.op == MATCH: + matches += cigar_aln.length + return matches + + def mismatches(self): + mismatches = 0 + for exon_aln in self.exon_alignments: + for cigar_aln in exon_aln.cigar_alns: + if cigar_aln.op == MM: + mismatches += cigar_aln.length + return mismatches + + def deletions(self): + deletions = 0 + for exon_aln in self.exon_alignments: + for cigar_aln in exon_aln.cigar_alns: + if cigar_aln.op == DEL: + deletions += cigar_aln.length + return deletions + + def gap_count(self): + gaps = 0 + for exon_aln in self.exon_alignments: + for cigar_aln in exon_aln.cigar_alns: + if cigar_aln.op == DEL or cigar_aln.op == INS: + gaps += 1 + return gaps + + def aln_length(self): + length = 0 + for exon_aln in self.exon_alignments: + for cigar_aln in exon_aln.cigar_alns: + length += cigar_aln.length + return length + + def identity_gap(self): + return f"{self.matches() / float(self.aln_length()):.6f}" + + def identity_ungap(self): + return f"{self.matches() / float(self.matches() + self.mismatches()):.6f}" + + def coverage(self): + return f"{(self.matches() + self.mismatches() + self.deletions()) / float(self.seq_length):.6f}" + + +logging.basicConfig(level=logging.INFO) +logger = logging.getLogger("alignment_metrics") + +p = re.compile("\d+[=DIX]") +MATCH = "=" +INS = "I" +DEL = "D" +MM = "X" + +TX_EXON_SET_SUMMARY_ALL_BUILD37_SQL = """ +select * +from uta_20210129b.tx_exon_set_summary_mv as mv +where mv.alt_aln_method in ('splign', 'splign-manual') and mv.tx_ac ~ 'N[MR]_*' and mv.tx_ac !~ '/' + and mv.alt_ac in ('NC_000001.10', 'NC_000002.11', 'NC_000003.11', 'NC_000004.11', 'NC_000005.9', 'NC_000006.11', + 'NC_000007.13', 'NC_000008.10', 'NC_000009.11', 'NC_000010.10', 'NC_000011.9', 'NC_000012.11', + 'NC_000013.10', 'NC_000014.8', 'NC_000015.9', 'NC_000016.9', 'NC_000017.10', 'NC_000018.9', + 'NC_000019.9', 'NC_000020.10', 'NC_000021.8', 'NC_000022.10', 'NC_000023.10', 'NC_000024.9' + ); +""" + +TX_EXON_SET_SUMMARY_SQL = """ +select mv.ends_i[mv.n_exons] as tx_length, * +from uta_20210129b.tx_exon_set_summary_mv as mv +where mv.tx_ac='{tx_ac}' and mv.alt_ac='{alt_ac}' and mv.alt_aln_method='{alt_aln_method}'; +""" + +TX_EXON_ALN_SQL = """ +select v.hgnc, v.tx_ac, v.alt_ac, v.alt_aln_method, v.alt_strand, v.ord, v.tx_start_i, v.tx_end_i, + v.alt_start_i, v.alt_end_i, v.cigar +from uta_20210129b.tx_exon_aln_v as v +where tx_ac='{tx_ac}' and alt_ac='{alt_ac}' and alt_aln_method='{alt_aln_method}' +order by ord; +""" + + +def parse_args(): + parser = argparse.ArgumentParser(description="Generate alignment metrics for transcript alignments") + parser.add_argument("output_file", type=str) + parser.add_argument("--db-url", default="postgresql://uta_admin@localhost/uta") + parser.add_argument("--schema-name", default="uta_20210129b") + return parser.parse_args() + + +def _get_cursor(con, schema_name): + cur = con.cursor(cursor_factory=psycopg2.extras.NamedTupleCursor) + cur.execute("set role uta_admin;") + cur.execute(f"set search_path = {schema_name}") + return cur + + +def _get_alignment(cur, tx_ac, alt_ac, alt_aln_method, aligned_exon_count): + # get transcript exon count + cur.execute( + TX_EXON_SET_SUMMARY_SQL.format(tx_ac=tx_ac, alt_ac=tx_ac, alt_aln_method="transcript") + ) + row = cur.fetchone() + + if row is None: + logger.warn(f"no transcript alignment found for {tx_ac} {alt_ac} {alt_aln_method}") + tx_exon_count = None + else: + tx_exon_count = row.n_exons + tx_seq_length = row.tx_length + + cur.execute( + TX_EXON_ALN_SQL.format( + tx_ac=tx_ac, alt_ac=alt_ac, alt_aln_method=alt_aln_method + ) + ) + rows = cur.fetchall() + tx_aln = None + + for row in rows: + if tx_aln is None: + tx_aln = TxAln( + hgnc=row.hgnc, + tx_ac=row.tx_ac, + seq_length=tx_seq_length, + exon_count=tx_exon_count, + alt_ac=row.alt_ac, + alt_aln_method=row.alt_aln_method, + alt_strand=row.alt_strand, + aligned_exon_count=aligned_exon_count, + ) + exon_aln = ExonAln( + tx_start_i=row.tx_start_i, + tx_end_i=row.tx_end_i, + alt_start_i=row.alt_start_i, + alt_end_i=row.alt_end_i, + cigar=row.cigar, + ) + for match in p.finditer(exon_aln.cigar): + cigar_aln = CigarAln(op=match.group()[-1], length=int(match.group()[:-1])) + exon_aln.cigar_alns.append(cigar_aln) + tx_aln.exon_alignments.append(exon_aln) + return tx_aln + + +def main(db_url, schema_name, output_file): + logger.info(f"connecting to {db_url}") + session = uta.connect(db_url) + + con = session.bind.pool.connect() + cur = _get_cursor(con, schema_name) + + # get tx_ac/alt_ac pairs + tx_alt_ac_pairs = [] + cur.execute(TX_EXON_SET_SUMMARY_ALL_BUILD37_SQL) + rows = cur.fetchall() + + for row in rows: + tx_alt_ac_pairs.append((row.tx_ac, row.alt_ac, row.alt_aln_method, row.n_exons)) + + logger.info(f"writing metrics to {output_file} for {len(tx_alt_ac_pairs)} transcript alignments") + with open(output_file, "w") as f_out: + f_out.write(TxAln.metrics_header()) + i = 0 + for tx_ac, alt_ac, alt_aln_method, aligned_exon_count in tx_alt_ac_pairs: + tx_aln = _get_alignment( + cur, tx_ac, alt_ac, alt_aln_method, aligned_exon_count + ) + f_out.write(tx_aln.to_metric_output_row()) + f_out.flush() + i += 1 + if i % 500 == 0: + logger.info(f" - {i} transcript alignments processed") + + +if __name__ == "__main__": + arguments = parse_args() + main(arguments.db_url, arguments.schema_name, arguments.output_file) From 93e79f7751e662d59bb0efc8763ea396f1394cec Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Sun, 5 May 2024 09:12:48 -0600 Subject: [PATCH 41/54] feat(IPVC-2408): Mito processing updates and bug fixes (#31) --- README.md | 4 ++-- docker-compose.yml | 2 +- sbin/ncbi_process_mito.py | 39 ++++++++++++++++++++++++++++++++++----- sbin/seqrepo-load | 8 +++++--- sbin/uta-diff | 3 ++- sbin/uta-load | 14 ++------------ src/uta/loading.py | 2 ++ 7 files changed, 48 insertions(+), 24 deletions(-) diff --git a/README.md b/README.md index 3acbe72..e79452b 100644 --- a/README.md +++ b/README.md @@ -347,14 +347,14 @@ See 2A for nuclear transcripts and 2B for mitochondrial transcripts. docker compose run ncbi-download docker compose run uta-extract docker compose run seqrepo-load -UTA_ETL_SKIP_GENE_LOAD=false docker compose run uta-load +docker compose run uta-load ``` #### 2B. Mitochondrial transcripts ``` docker compose run mito-extract docker compose run seqrepo-load -UTA_ETL_SKIP_GENE_LOAD=true docker compose run uta-load +docker compose run uta-load ``` #### 2C. Manual splign transcripts diff --git a/docker-compose.yml b/docker-compose.yml index c7c9f6e..b7962ac 100644 --- a/docker-compose.yml +++ b/docker-compose.yml @@ -42,7 +42,7 @@ services: network_mode: host uta-load: image: uta-update - command: sbin/uta-load ${UTA_ETL_OLD_UTA_VERSION} /ncbi-dir /uta-load/work /uta-load/logs ${UTA_ETL_SKIP_GENE_LOAD} + command: sbin/uta-load ${UTA_ETL_OLD_UTA_VERSION} /ncbi-dir /uta-load/work /uta-load/logs depends_on: uta: condition: service_healthy diff --git a/sbin/ncbi_process_mito.py b/sbin/ncbi_process_mito.py index ccd1d69..51190f7 100755 --- a/sbin/ncbi_process_mito.py +++ b/sbin/ncbi_process_mito.py @@ -58,7 +58,7 @@ import importlib_resources import logging import logging.config -from typing import Dict, List, Optional +from typing import Dict, Iterable, List, Optional from Bio.Seq import Seq import Bio.SeqIO @@ -68,6 +68,7 @@ from more_itertools import one from uta.formats.geneaccessions import GeneAccessions, GeneAccessionsWriter +from uta.formats.geneinfo import GeneInfo, GeneInfoWriter from uta.formats.seqinfo import SeqInfo, SeqInfoWriter from uta.formats.txinfo import TxInfo, TxInfoWriter from uta.formats.exonset import ExonSet, ExonSetWriter @@ -79,6 +80,9 @@ class MitoGeneData: gene_id: int gene_symbol: str name: str + synonym: str + xrefs: List[str] + type: str tx_ac: str tx_seq: str tx_start: int @@ -110,7 +114,7 @@ def alt_exons_se_i(self) -> str: logger = logging.getLogger(__name__) -def parse_args(): +def parse_args() -> argparse.Namespace: parser = argparse.ArgumentParser(description=__doc__) parser.add_argument("accession", type=str) parser.add_argument("--output-dir", "-o", default=".", type=str) @@ -166,7 +170,7 @@ def parse_nomenclature_value(gb_feature: SeqFeature) -> Dict[str, str]: return nomenclature_results -def get_mito_genes(gbff_filepath: str): +def get_mito_genes(gbff_filepath: str) -> Iterable[MitoGeneData]: logger.info(f"processing NCBI GBFF file from {gbff_filepath}") with open(gbff_filepath) as fh: # Bio.SeqIO.parse(fh, "gb") returns an empty iterator for .fna files and does not fail @@ -199,11 +203,14 @@ def get_mito_genes(gbff_filepath: str): # retrieve sequence, and reverse compliment if on reverse strand ac = f"{record.id}_{feature.location.start:05}_{feature.location.end:05}" feature_seq = record.seq[feature_start:feature_end] + gene_synonym = feature.qualifiers.get("gene_synonym", "") + type = feature.type if feature.location.strand == -1: feature_seq = feature_seq.reverse_complement() if feature.type == "CDS": # override defaults for CDS features + type = "protein-coding" pro_ac = one(feature.qualifiers["protein_id"]) pro_seq = str(one(feature.qualifiers["translation"])) transl_table = one(feature.qualifiers["transl_table"]) @@ -219,6 +226,9 @@ def get_mito_genes(gbff_filepath: str): gene_id=gene_id, gene_symbol=hgnc, name=name, + synonym=gene_synonym, + xrefs=[f"{k}:{v}" for k, v in xrefs.items()], + type=type, tx_ac=ac, tx_seq=str(feature_seq), tx_start=0, @@ -234,7 +244,7 @@ def get_mito_genes(gbff_filepath: str): ) -def main(ncbi_accession: str, output_dir: str): +def main(ncbi_accession: str, output_dir: str) -> None: # get input files input_files = download_mito_files(output_dir=output_dir, accession=ncbi_accession) @@ -242,7 +252,26 @@ def main(ncbi_accession: str, output_dir: str): mito_genes = [mg for mf in input_files.values() for mg in get_mito_genes(mf)] logger.info(f"found {len(mito_genes)} genes from parsing {input_files['gbff']}") - # write gene accessions + # write gene information + with gzip.open(f"{output_dir}/geneinfo.gz", "wt") as o_file: + giw = GeneInfoWriter(o_file) + for mg in mito_genes: + giw.write( + GeneInfo( + mg.gene_id, + mg.gene_symbol, + 9606, + mg.gene_symbol, + "", + mg.synonym, + mg.type, + mg.name, + mg.name, + mg.xrefs, + ) + ) + + # write gene accession associations with gzip.open(f"{output_dir}/assocacs.gz", "wt") as o_file: gaw = GeneAccessionsWriter(o_file) for mg in mito_genes: diff --git a/sbin/seqrepo-load b/sbin/seqrepo-load index adadc04..3eb3345 100755 --- a/sbin/seqrepo-load +++ b/sbin/seqrepo-load @@ -13,9 +13,11 @@ then exit 1 fi -## Load SeqRepo with new sequences +# find all fasta files in the working directory +mapfile -t FASTA_FILES < <(find "$sequence_dir" -type f -name "*.f[an]a*") + +# Load SeqRepo with new sequences seqrepo --root-directory "$seqrepo_root" \ load -n NCBI --instance-name "$seqrepo_version" \ - "$sequence_dir"/*.fna.gz \ - "$sequence_dir"/*.faa.gz 2>& 1 | \ + "${FASTA_FILES[@]}" 2>& 1 | \ tee "$log_dir/seqrepo-load.log" diff --git a/sbin/uta-diff b/sbin/uta-diff index 7b35158..d89798d 100755 --- a/sbin/uta-diff +++ b/sbin/uta-diff @@ -15,6 +15,7 @@ cmp_cols.update({ "associated_accessions": "tx_ac pro_ac origin".split(), "exon_aln": "exon_aln_id tx_exon_id alt_exon_id cigar added".split(), "gene": "gene_id".split(), + "seq_anno": "seq_anno_id seq_id origin_id ac added".split(), "transcript": "ac".split(), }) @@ -41,7 +42,7 @@ def cmp1(con, tbl, s1, s2): if __name__ == "__main__": - logging.basicConfig(level=logging.DEBUG) + logging.basicConfig(level=logging.INFO) logger = logging.getLogger() url = "postgresql://uta_admin@localhost/uta" diff --git a/sbin/uta-load b/sbin/uta-load index df0f3db..06daec8 100755 --- a/sbin/uta-load +++ b/sbin/uta-load @@ -5,7 +5,6 @@ # ncbi_dir is where the script looks for NCBI data files. # working_dir stores intermediate data files and the final database dump. # log_dir stores log files. -# skip_load_genes, if truthy, will skip the gene loading step # Note that the uta loading code uses the seqrepo location defined in the conf files, under [sequences].seqrepo. @@ -15,7 +14,6 @@ source_uta_v=$1 ncbi_dir=$2 working_dir=$3 log_dir=$4 -skip_load_genes=$5 if [ -z "$source_uta_v" ] || [ -z "$ncbi_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] then @@ -43,13 +41,8 @@ sbin/assoc-acs-merge "$working_dir/assocacs.gz" | gzip -c > "$working_dir/assoc- tee "$log_dir/assoc-acs-merge.log" # Load genes into gene table. -if [ "$skip_load_genes" = "true" ] -then - echo "Skipping load-geneinfo" -else - uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$working_dir/geneinfo.gz" 2>&1 | \ - tee "$log_dir/load-geneinfo.log" -fi +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-geneinfo "$working_dir/geneinfo.gz" 2>&1 | \ + tee "$log_dir/load-geneinfo.log" # Load accessions into associated_accessions table. uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-assoc-ac "$working_dir/assoc-ac.gz" 2>&1 | \ @@ -73,6 +66,3 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | ### run diff sbin/uta-diff "$source_uta_v" "$loading_uta_v" - -### psql_dump -pg_dump -U uta_admin -h localhost -d uta -t "$loading_uta_v.gene" | gzip -c > "$working_dir/uta.pgd.gz" diff --git a/src/uta/loading.py b/src/uta/loading.py index 90cdc2f..9590c49 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -523,6 +523,7 @@ def _upsert_seq(si): session.merge(u_seqanno) else: # create the new annotation + logger.debug("creating seq_anno({si.origin},{si.ac},{si.md5})".format(si=si)) u_seqanno = usam.SeqAnno(origin_id=u_ori.origin_id, seq_id=si.md5, ac=si.ac, descr=si.descr) session.add(u_seqanno) @@ -533,6 +534,7 @@ def _upsert_seq(si): logger.info("{n_created} annotations created/{i_md5} sequences seen ({p:.1f}%)/{n_rows} sequences total".format( n_created=n_created, i_md5=i_md5, n_rows=n_rows, md5=md5, p=i_md5 / n_rows * 100)) session.commit() + session.commit() def load_sequences(session, opts, cf): From 4cb5f4f837c81171d278344c93751f4ef86bf86b Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Wed, 8 May 2024 10:27:45 -0600 Subject: [PATCH 42/54] feat(IPVC-2386): Backfill transl_excepts (#32) --- Dockerfile | 1 + docker-compose.yml | 2 - etc/ncbi-files.txt | 9 - .../docker-compose-backfill.yml | 14 ++ .../ncbi_extract_gbff.py | 196 ++++++++++++++++++ .../uta-extract-historical | 52 +++++ sbin/exonset-to-seqinfo | 14 +- sbin/filter_exonset_transcripts.py | 4 +- sbin/ncbi-parse-gbff | 12 +- sbin/ncbi_parse_genomic_gff.py | 5 +- src/uta/formats/txinfo.py | 13 ++ src/uta/loading.py | 47 ++++- src/uta/parsers/seqrecord.py | 129 ++++++++++-- tests/test_filter_exonset_transcripts.py | 4 +- tests/test_uta_parsers_seqrecord.py | 104 ++++++++-- 15 files changed, 539 insertions(+), 67 deletions(-) create mode 100644 misc/refseq-historical-backfill/docker-compose-backfill.yml create mode 100755 misc/refseq-historical-backfill/ncbi_extract_gbff.py create mode 100755 misc/refseq-historical-backfill/uta-extract-historical diff --git a/Dockerfile b/Dockerfile index 4b83e36..34d9a05 100644 --- a/Dockerfile +++ b/Dockerfile @@ -17,6 +17,7 @@ RUN pip install pysam WORKDIR /opt/repos/uta/ COPY pyproject.toml ./ COPY etc ./etc +COPY misc ./misc COPY sbin ./sbin COPY src ./src RUN pip install -e .[dev] diff --git a/docker-compose.yml b/docker-compose.yml index b7962ac..aa4081e 100644 --- a/docker-compose.yml +++ b/docker-compose.yml @@ -16,7 +16,6 @@ services: command: sbin/uta-extract /ncbi-dir /uta-extract/work /uta-extract/logs volumes: - ${UTA_ETL_NCBI_DIR}:/ncbi-dir - - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo - ${UTA_ETL_WORK_DIR}:/uta-extract/work - ${UTA_ETL_LOG_DIR}:/uta-extract/logs working_dir: /opt/repos/uta @@ -47,7 +46,6 @@ services: uta: condition: service_healthy volumes: - - ${UTA_ETL_NCBI_DIR}:/ncbi-dir - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo - ${UTA_ETL_WORK_DIR}:/uta-load/work - ${UTA_ETL_LOG_DIR}:/uta-load/logs diff --git a/etc/ncbi-files.txt b/etc/ncbi-files.txt index 6cb31ef..2b9bfb6 100644 --- a/etc/ncbi-files.txt +++ b/etc/ncbi-files.txt @@ -16,11 +16,6 @@ # │ └── GCF_000001405.25_GRCh37.p13_genomic.gff.gz # └── refseq # └── H_sapiens -# ├── historical -# │ └── GRCh38 -# │ └── GCF_000001405.40-RS_2023_03_historical -# │ ├── GCF_000001405.40-RS_2023_03_knownrefseq_alns.gff.gz -# │ └── GCF_000001405.40-RS_2023_03_knownrefseq_rna.gbff.gz # └── mRNA_Prot # ├── human.1.protein.faa.gz # ├── human.1.rna.fna.gz @@ -36,10 +31,6 @@ refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz refseq/H_sapiens/mRNA_Prot/human.*.rna.fna.gz refseq/H_sapiens/mRNA_Prot/human.*.protein.faa.gz -## Historical RefSeq alignments -refseq/H_sapiens/historical/GRCh38/GCF_000001405.40-RS_2023_03_historical/GCF_000001405.40-RS_2023_03_knownrefseq_alns.gff.gz -refseq/H_sapiens/historical/GRCh38/GCF_000001405.40-RS_2023_03_historical/GCF_000001405.40-RS_2023_03_knownrefseq_rna.gbff.gz - ## Genome build and alignment data # Build 37 genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405.25_GRCh37.p13/GCF_000001405.25_GRCh37.p13_assembly_report.txt diff --git a/misc/refseq-historical-backfill/docker-compose-backfill.yml b/misc/refseq-historical-backfill/docker-compose-backfill.yml new file mode 100644 index 0000000..6e773b0 --- /dev/null +++ b/misc/refseq-historical-backfill/docker-compose-backfill.yml @@ -0,0 +1,14 @@ +# docker compose file for the RefSeq historical backfill procedure + +version: '3' + +services: + uta-extract-historical: + image: uta-update + command: misc/refseq-historical-backfill/uta-extract-historical /ncbi-dir /uta-extract/work /uta-extract/logs + volumes: + - ${UTA_ETL_NCBI_DIR}:/ncbi-dir + - ${UTA_ETL_WORK_DIR}:/uta-extract/work + - ${UTA_ETL_LOG_DIR}:/uta-extract/logs + working_dir: /opt/repos/uta + network_mode: host diff --git a/misc/refseq-historical-backfill/ncbi_extract_gbff.py b/misc/refseq-historical-backfill/ncbi_extract_gbff.py new file mode 100755 index 0000000..4b877e1 --- /dev/null +++ b/misc/refseq-historical-backfill/ncbi_extract_gbff.py @@ -0,0 +1,196 @@ +""" +Extract and write all files needed by UTA load, except alt accession exonsets (aka, alignments). From a single +GBFF file we can create dna fasta, protein fasta, associated accessions, geneinfo, and txinfo files. +""" +import argparse +import gzip +import importlib_resources +import io +import logging +import logging.config +from collections import Counter +from contextlib import ExitStack +from typing import Iterable + +from Bio.Seq import Seq +import Bio.SeqIO +from Bio.SeqRecord import SeqRecord + +from uta.formats.geneaccessions import GeneAccessions, GeneAccessionsWriter +from uta.formats.geneinfo import GeneInfo, GeneInfoWriter +from uta.formats.txinfo import TxInfo, TxInfoWriter +from uta.parsers.seqrecord import SeqRecordFacade, SeqRecordFeatureError +from uta.tools.file_utils import open_file + + +logging_conf_fn = importlib_resources.files("uta").joinpath("etc/logging.conf") +logging.config.fileConfig(logging_conf_fn) +logging.getLogger().setLevel(logging.INFO) +logger = logging.getLogger(__name__) + + +def parse_args(): + ap = argparse.ArgumentParser( + description=__doc__, + ) + ap.add_argument("GBFF_FILES", nargs="+") + ap.add_argument("--origin", "-o", default="NCBI") + ap.add_argument("--prefix", "-p", default="") + ap.add_argument("--output_dir", "-d", default=".", type=str) + opts = ap.parse_args() + return opts + + +def main(gbff_files: Iterable, origin: str, prefix: str, output_dir: str) -> None: + if prefix: + prefix = f"{prefix}." + + # setup context managers for file writers + with ExitStack() as stack: + # DNA fasta file + dna_fasta_fh = stack.enter_context( + io.TextIOWrapper( + gzip.open(f"{output_dir}/{prefix}rna.fna.gz", "wb"), encoding="utf-8" + ) + ) + + # Protein fasta file + protein_fasta_fh = stack.enter_context( + io.TextIOWrapper( + gzip.open(f"{output_dir}/{prefix}protein.faa.gz", "wb"), + encoding="utf-8", + ) + ) + + geneinfo_fh = stack.enter_context( + io.TextIOWrapper( + gzip.open(f"{output_dir}/{prefix}geneinfo.gz", "wb"), encoding="utf-8" + ) + ) + geneinfo_writer = GeneInfoWriter(geneinfo_fh) + + txinfo_fh = stack.enter_context( + io.TextIOWrapper( + gzip.open(f"{output_dir}/{prefix}txinfo.gz", "w"), encoding="utf-8" + ) + ) + txinfo_writer = TxInfoWriter(txinfo_fh) + + assocacs_fh = stack.enter_context( + io.TextIOWrapper( + gzip.open(f"{output_dir}/{prefix}assocacs.gz", "w"), encoding="utf-8" + ) + ) + assocacs_writer = GeneAccessionsWriter(assocacs_fh) + + total_genes = set() + total_skipped = set() + all_prefixes = Counter() + for gbff_fn in gbff_files: + logger.info(f"Processing {gbff_fn}") + gbff_file_handler = stack.enter_context(open_file(gbff_fn)) + i = 0 + genes = set() + skipped = set() + prefixes = Counter() + for r in Bio.SeqIO.parse(gbff_file_handler, "gb"): + srf = SeqRecordFacade(r) + + # skip transcripts where the exon structure is unknown + if not srf.exons_se_i: + skipped.add(srf.id) + continue + + prefixes.update([srf.id[:2]]) + try: + fna_record = SeqRecord( + Seq(srf.feature_seq), id=srf.id, description="" + ) + dna_fasta_fh.write(fna_record.format("fasta")) + + if srf.gene_id not in genes: + geneinfo_writer.write( + GeneInfo( + gene_id=srf.gene_id, + gene_symbol=srf.gene_symbol, + tax_id="9606", + hgnc=srf.gene_symbol, + maploc="", + aliases=srf.gene_synonyms, + type=srf.gene_type, + summary="", + descr="", + xrefs=srf.db_xrefs, + ) + ) + + txinfo_writer.write( + TxInfo( + origin=origin, + ac=srf.id, + gene_id=srf.gene_id, + gene_symbol=srf.gene_symbol, + cds_se_i=TxInfo.serialize_cds_se_i(srf.cds_se_i), + exons_se_i=TxInfo.serialize_exons_se_i(srf.exons_se_i), + transl_except=TxInfo.serialize_transl_except( + srf.transl_except + ), + ) + ) + + # only write cds features for protein-coding transcripts + if srf.cds_feature is not None: + pro_record = SeqRecord( + Seq(srf.cds_translation), + id=srf.cds_protein_id, + description=srf.cds_product, + ) + protein_fasta_fh.write(pro_record.format("fasta")) + + assocacs_writer.write( + GeneAccessions( + origin=origin, + gene_id=srf.gene_id, + gene_symbol=srf.gene_symbol, + tx_ac=srf.id, + pro_ac=srf.cds_protein_id, + ) + ) + + genes.add(srf.gene_id) + i += 1 + if i % 5000 == 0: + logger.info( + " - {ng} genes in {fn} ({c}); {s} transcripts skipped".format( + ng=len(genes), + fn=gbff_fn, + c=prefixes, + s=len(skipped), + ) + ) + except SeqRecordFeatureError as e: + logger.error(f"SeqRecordFeatureError processing {r.id}: {e}") + raise + except ValueError as e: + logger.error(f"ValueError processing {r.id}: {e}") + raise + + + logger.info( + "{ng} genes in {fn} ({c}); {s} transcripts skipped".format( + ng=len(genes), fn=gbff_fn, c=prefixes, s=len(skipped) + ) + ) + total_genes ^= genes + total_skipped ^= skipped + all_prefixes += prefixes + logger.info( + "{ng} genes in {nf} ({c}); {s} transcripts skipped".format( + ng=len(total_genes), nf=len(gbff_files), c=all_prefixes, s=len(total_skipped) + ) + ) + + +if __name__ == "__main__": + args = parse_args() + main(args.GBFF_FILES, args.origin, args.prefix, args.output_dir) diff --git a/misc/refseq-historical-backfill/uta-extract-historical b/misc/refseq-historical-backfill/uta-extract-historical new file mode 100755 index 0000000..1586cfe --- /dev/null +++ b/misc/refseq-historical-backfill/uta-extract-historical @@ -0,0 +1,52 @@ +#!/usr/bin/env bash + +# Download, then extract intermediate files out of the NCBI historical alignment files. + +set -e + +ncbi_dir=$1 +working_dir=$2 +log_dir=$3 + +if [ -z "$ncbi_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] +then + echo 'Usage: sbin/uta-extract-historical ' + exit 1 +fi + +download_ncbi_file () { + download_path=$1 + download_dir=$2 + + download_module="${download_path%%/*}" + download_source="ftp.ncbi.nlm.nih.gov::$download_path" + download_destination="$download_dir/$download_module" + + mkdir -p $download_destination + echo "Downloading $download_source to $download_destination" + rsync --no-motd -DHPRprtv "$download_source" "$download_destination" +} + +relative_path="refseq/H_sapiens/historical/GRCh38/GCF_000001405.40-RS_2023_03_historical" + +# download historical genbank file +file_path="$relative_path/GCF_000001405.40-RS_2023_03_knownrefseq_rna.gbff.gz" +download_ncbi_file $file_path $ncbi_dir + +# download historical gff file +file_path="$relative_path/GCF_000001405.40-RS_2023_03_knownrefseq_alns.gff.gz" +download_ncbi_file $file_path $ncbi_dir + +# extract intermediate files from genbank file +python misc/refseq-historical-backfill/ncbi_extract_gbff.py \ + "$ncbi_dir/$relative_path/GCF_000001405.40-RS_2023_03_knownrefseq_rna.gbff.gz" \ + --output_dir "$working_dir" 2>&1 | tee "$log_dir/ncbi-parse-historical-ggbb.log" + +# extract exonset intermediate file from gff file +python sbin/ncbi_parse_genomic_gff.py "$ncbi_dir/$relative_path/GCF_000001405.40-RS_2023_03_knownrefseq_alns.gff.gz" | \ + gzip -c > "$working_dir/unfiltered_exonsets.gz" 2>&1 | tee "$log_dir/ncbi-parse-historical-gff.log" + +# filter exonset alignments by txinfo +sbin/filter_exonset_transcripts.py --tx-info "$working_dir/txinfo.gz" --exonsets "$working_dir/unfiltered_exonsets.gz" \ + --missing-ids "$working_dir/filtered_tx_acs.txt" | gzip -c > "$working_dir/exonsets.gz" 2>&1 | \ + tee "$log_dir/filter_exonset_transcripts.log" diff --git a/sbin/exonset-to-seqinfo b/sbin/exonset-to-seqinfo index 33b3cba..21544f3 100755 --- a/sbin/exonset-to-seqinfo +++ b/sbin/exonset-to-seqinfo @@ -5,16 +5,15 @@ import argparse import configparser as ConfigParser import gzip +import importlib_resources import itertools import logging import logging.config -import pkg_resources import re import sys from bioutils.digests import seq_md5 from biocommons.seqrepo import SeqRepo -# from multifastadb import MultiFastaDB from uta.formats.exonset import ExonSetReader from uta.formats.seqinfo import SeqInfo, SeqInfoWriter @@ -32,16 +31,15 @@ def parse_args(argv): required=True) ap.add_argument("--conf", default=[ - pkg_resources.resource_filename("uta", "../../etc/global.conf")] - ) + importlib_resources.files("uta").joinpath("../../etc/global.conf") + ]) opts = ap.parse_args(argv) return opts if __name__ == "__main__": - logging_conf_fn = pkg_resources.resource_filename( - "uta", "etc/logging.conf") + logging_conf_fn = importlib_resources.files("uta").joinpath("etc/logging.conf") logging.config.fileConfig(logging_conf_fn) logger = logging.getLogger(__name__) logger.setLevel(logging.INFO) @@ -53,15 +51,13 @@ if __name__ == "__main__": cf = ConfigParser.ConfigParser() for conf_fn in opts.conf: cf.read_file(open(conf_fn)) - logger.info("loaded " + conf_fn) + logger.info("loaded " + str(conf_fn)) in_fn = opts.FILES[0] in_fh = gzip.open(in_fn, 'rt') if in_fn.endswith(".gz") else open(in_fn) esr = ExonSetReader(in_fh) logger.info("opened " + in_fn) - #fa_dirs = cf.get("sequences", "fasta_directories").strip().splitlines() - #mfdb = MultiFastaDB(fa_dirs, use_meta_index=True) sr_dir = cf.get("sequences", "seqrepo") sr = SeqRepo(root_dir=sr_dir) logger.info("Opened sequence directories: " + sr_dir) diff --git a/sbin/filter_exonset_transcripts.py b/sbin/filter_exonset_transcripts.py index b3d1fc2..1d97cc4 100755 --- a/sbin/filter_exonset_transcripts.py +++ b/sbin/filter_exonset_transcripts.py @@ -28,10 +28,10 @@ def filter_exonset(exonset_file, transcript_ids, missing_ids_file): if exonset.tx_ac in transcript_ids: esw.write(exonset) else: - logger.warning(f"Exon set transcript {exonset.tx_ac} not found in txinfo file. Filtering out.") + logger.debug(f"Exon set transcript {exonset.tx_ac} not found in txinfo file. Filtering out.") writer_missing.writerow([exonset.tx_ac]) missing_acs.add(exonset.tx_ac) - logger.info(f"Filtered out exon sets for {len(missing_acs)} transcript(s): {','.join(missing_acs)}") + logger.info(f"Filtered out exon sets for {len(missing_acs)} transcript(s)") def main(): diff --git a/sbin/ncbi-parse-gbff b/sbin/ncbi-parse-gbff index 3b3b7ff..9850d4c 100755 --- a/sbin/ncbi-parse-gbff +++ b/sbin/ncbi-parse-gbff @@ -27,10 +27,10 @@ from __future__ import division, unicode_literals import argparse from collections import Counter import gzip +import importlib_resources import io import logging import logging.config -import pkg_resources import re import sys @@ -38,7 +38,7 @@ import Bio.SeqIO from bioutils.digests import seq_md5 from uta.formats.txinfo import TxInfo, TxInfoWriter -from uta.parsers.seqrecord import SeqRecordFacade +from uta.parsers.seqrecord import SeqRecordFacade, SeqRecordFeatureError origin = "NCBI" @@ -89,8 +89,7 @@ def gbff_block_reader(it): if __name__ == "__main__": - logging_conf_fn = pkg_resources.resource_filename( - "uta", "etc/logging.conf") + logging_conf_fn = importlib_resources.files("uta").joinpath("etc/logging.conf") logging.config.fileConfig(logging_conf_fn) logging.getLogger().setLevel(logging.INFO) logger = logging.getLogger(__name__) @@ -112,14 +111,13 @@ if __name__ == "__main__": if srf.id.partition("_")[0] not in ["NM", "NR"]: skipped_ids.add(srf.id) continue - cds_se_i = srf.cds_se_i ti = TxInfo( ac=srf.id, origin=opts.origin, gene_id=srf.gene_id, gene_symbol=srf.gene_symbol, - cds_se_i=None if cds_se_i is None else "{},{}".format(*cds_se_i), - exons_se_i=";".join(["{},{}".format(*ese) for ese in srf.exons_se_i]), + cds_se_i=TxInfo.serialize_cds_se_i(srf.cds_se_i), + exons_se_i=TxInfo.serialize_exons_se_i(srf.exons_se_i), transl_except=TxInfo.serialize_transl_except(srf.transl_except), ) tiw.write(ti) diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index 728e8a5..d784a10 100755 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -24,14 +24,13 @@ """ import argparse +import importlib_resources import logging.config import sys from collections import defaultdict from dataclasses import dataclass from typing import List, Optional -import pkg_resources - from uta.formats.exonset import ExonSet, ExonSetWriter from uta.tools.file_utils import open_file @@ -129,7 +128,7 @@ def get_zero_based_exon_ranges(transcript_exons: List[GFFRecord]) -> str: if __name__ == "__main__": - logging_conf_fn = pkg_resources.resource_filename("uta", "etc/logging.conf") + logging_conf_fn = importlib_resources.files("uta").joinpath("etc/logging.conf") logging.config.fileConfig(logging_conf_fn) logging.getLogger().setLevel(logging.INFO) logger = logging.getLogger(__name__) diff --git a/src/uta/formats/txinfo.py b/src/uta/formats/txinfo.py index c869c42..9e812bf 100644 --- a/src/uta/formats/txinfo.py +++ b/src/uta/formats/txinfo.py @@ -19,6 +19,19 @@ def serialize_transl_except(transl_except_list: Optional[List[str]]) -> Optional else: return ";".join(transl_except_list) + @staticmethod + def serialize_cds_se_i(cds_se_i: Optional[tuple]) -> Optional[str]: + """Helper for formatting cds_se_i tuple as a string.""" + if cds_se_i is None: + return None + else: + return "{},{}".format(*cds_se_i) + + @staticmethod + def serialize_exons_se_i(exons_se_i: List[tuple]) -> str: + """Helper for formatting exons_se_i list as a string.""" + return ";".join(["{},{}".format(*ese) for ese in exons_se_i]) + class TxInfoWriter(csv.DictWriter): diff --git a/src/uta/loading.py b/src/uta/loading.py index 9590c49..27187a8 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -346,7 +346,7 @@ def load_geneinfo(session, opts, cf): type=gi.type, xrefs=gi.xrefs, )) - logger.info("Added {gi.gene_symbol}: {gi.gene_id} ({gi.summary})".format(gi=gi)) + logger.debug("Added {gi.gene_symbol}: {gi.gene_id} ({gi.summary})".format(gi=gi)) session.commit() @@ -656,6 +656,29 @@ def _fetch_origin_by_name(name): u_tx = None n_cds_changed += 1 + if ti.transl_except: + # if the transl_except exists, make sure it exists in the database. + te_list = _create_translation_exceptions( + transcript=ti.ac, transl_except_list=ti.transl_except.split(";") + ) + for te_data in te_list: + te, created = _get_or_insert( + session=session, + table=usam.TranslationException, + row=te_data, + row_identifier=("tx_ac", "start_position", "end_position", "amino_acid"), + ) + if created: + logger.info( + f"TranslationException added: {te.tx_ac}, {te.start_position}, {te.end_position}, {te.amino_acid}" + ) + else: + logger.info( + f"TranslationException already exists: {te.tx_ac}, {te.start_position}, {te.end_position}, {te.amino_acid}" + ) + + + # state: u_tx is set if a transcript was found and was # unchanged, or None if 1) no such was found or 2) was found # and had updated CDS coords. @@ -836,7 +859,7 @@ def _upsert_exon_set_record(session, tx_ac, alt_ac, strand, method, ess): returns tuple of (new_record, old_record) as follows: (new, None) -- no prior record; new was inserted - (None, old) -- prior record and unchaged; nothing was inserted + (None, old) -- prior record and unchanged; nothing was inserted (new, old) -- prior record existed and was changed """ @@ -870,9 +893,29 @@ def _upsert_exon_set_record(session, tx_ac, alt_ac, strand, method, ess): usam.ExonSet.alt_aln_method == alt_aln_method_with_hash, ) if existing.count() == 1: + logger.warning( + "Exon set {tx_ac}/{alt_ac} with method {method} already exists with hash {esh}".format( + tx_ac=tx_ac, + alt_ac=alt_ac, + method=method, + esh=alt_aln_method_with_hash, + ) + ) return (None, existing[0]) # update aln_method to add a unique exon set hash based on the *existing* exon set string + logger.warning( + "Exon set {tx_ac}/{alt_ac} with method {method} already exists, but with different exons; " + "existing exon set: {es_ess}; new exon set: {ess}; updated alt_aln_method of exonset to " + "{alt_aln_method_with_hash}".format( + tx_ac=tx_ac, + alt_ac=alt_ac, + method=method, + es_ess=es_ess, + ess=ess, + alt_aln_method_with_hash=alt_aln_method_with_hash, + ) + ) es.alt_aln_method = alt_aln_method_with_hash session.flush() old_es = es diff --git a/src/uta/parsers/seqrecord.py b/src/uta/parsers/seqrecord.py index 3497f4d..dd030e2 100644 --- a/src/uta/parsers/seqrecord.py +++ b/src/uta/parsers/seqrecord.py @@ -11,7 +11,6 @@ class SeqRecordFeatureError(Exception): class SeqRecordFacade: - def __init__(self, seqrecord: Bio.SeqRecord.SeqRecord): self._sr = seqrecord @@ -20,32 +19,57 @@ def features_by_type(self) -> dict[str, list]: result = defaultdict(list) for feat in self._sr.features: result[feat.type].append(feat) - self.validate_features_by_type(result) return result - @staticmethod - def validate_features_by_type(features: dict[str, list]) -> None: - """Raise exceptions if feature mapping is invalid.""" - if "CDS" in features and len(features["CDS"]) > 1: - raise SeqRecordFeatureError("Expected one `CDS` feature at most") - if "gene" not in features or len(features["gene"]) != 1: - raise SeqRecordFeatureError("Expected exactly one `gene` feature") - @cached_property def cds_feature(self) -> Optional[SeqFeature]: """ Returns the CDS feature for any coding transcript, None for any non-coding transcript. + Some NCBI records will contain multiple CDS features. In these one CDS describes a protein + with accession and protein sequence, the other CDS features describes a pseudogene. This method + will preferentially choose the CDS feature with a protein sequence. + Example: + CDS 422..778 + /gene="C6orf119" + /gene_synonym="dJ427A4.2" + /codon_start=1 + /product="chromosome 6 open reading frame 119" + /protein_id="NP_001012240.1" + /db_xref="GI:59276067" + /db_xref="GeneID:353267" + /translation="MTDTAEAVPNFEEMFASRFTENDKEYQEYLKRPPESPPIVEEWN + SRAGGNQRNRGNRLQDNRQFRGRDNRWGWPSDNRSNQWHGRSWGNNYPQHRQEPYYPQ + QYGHYGYNQRPPYGYY" + CDS 422..775 + /locus_tag="RP3-427A4.2-001" + /note="match: proteins: Q9BTL3 Q9CQY2 Q9CWI1" + /pseudo + /codon_start=1 + /product="Novel pseudogene" """ cds_features = self.features_by_type.get("CDS") if cds_features is None: return None else: - return cds_features[0] + # Prefer CDS with protein accession and translated sequence. + translated_cds_features = [ + f + for f in cds_features + if all([key in f.qualifiers for key in ("protein_id", "translation")]) + ] + if len(translated_cds_features) != 1: + raise SeqRecordFeatureError("Expected one `CDS` feature at most") + return translated_cds_features[0] @cached_property def gene_feature(self) -> SeqFeature: """Returns the gene feature, which should exist for all transcripts.""" - return self.features_by_type.get("gene")[0] + gene_features = self.features_by_type.get("gene") + if gene_features is None or len(gene_features) != 1: + raise SeqRecordFeatureError(f"Expected exactly one `gene` feature, for {self.id} " + f"found {len(gene_features) if gene_features is not None else None}") + + return gene_features[0] @property def id(self): @@ -55,6 +79,38 @@ def id(self): def gene_symbol(self): return self.gene_feature.qualifiers["gene"][0] + @property + def gene_synonyms(self): + if "gene_synonym" in self.gene_feature.qualifiers: + return [gs.strip() for gs in self.gene_feature.qualifiers["gene_synonym"][0].split(";")] + else: + return [] + + @property + def gene_type(self): + if self.cds_feature: + return "protein-coding" + elif "ncRNA" in self.features_by_type: + return "ncRNA" + elif "pseudo" in self.features_by_type: + return "pseudo" + elif "rRNA" in self.features_by_type: + return "rRNA" + elif "snoRNA" in self.features_by_type: + return "snoRNA" + elif "tRNA" in self.features_by_type: + return "tRNA" + elif "scRNA" in self.features_by_type: + return "scRNA" + elif "snRNA" in self.features_by_type: + return "snRNA" + elif "misc_RNA" in self.features_by_type: + return "misc_RNA" + elif "other" in self.features_by_type: + return "other" + else: + return "unknown" + @property def gene_id(self): # db_xref="GeneID:1234" @@ -63,21 +119,54 @@ def gene_id(self): assert len(gene_ids) == 1 return gene_ids[0] + @property + def db_xrefs(self): + """ + gene 1..4577 + /gene="A2M" + /gene_synonym="DKFZp779B086; FWP007; S863-7" + /db_xref="GeneID:2" + /db_xref="HPRD:00072" + /db_xref="MIM:103950" + """ + db_xrefs = self.gene_feature.qualifiers["db_xref"] + return [xref for xref in db_xrefs] + @property def cds_se_i(self): - if self.features_by_type.get("CDS"): - cds_feature = self.features_by_type.get("CDS")[0] - return (cds_feature.location.start.real, cds_feature.location.end.real) + if self.cds_feature is not None: + return self.cds_feature.location.start.real, self.cds_feature.location.end.real + else: + return None + + @property + def cds_product(self): + if self.cds_feature is not None: + return self.cds_feature.qualifiers["product"][0] + else: + return None + + @property + def cds_protein_id(self): + if self.cds_feature is not None: + return self.cds_feature.qualifiers["protein_id"][0] + else: + return None + + @property + def cds_translation(self): + if self.cds_feature is not None: + return str(self.cds_feature.qualifiers["translation"][0]) else: return None @property def exons_se_i(self): + se_i = [] if "exon" in self.features_by_type: exons = self.features_by_type["exon"] - else: - exons = [self.gene_feature] - return [(f.location.start.real, f.location.end.real) for f in exons] + se_i = [(f.location.start.real, f.location.end.real) for f in exons] + return se_i @property def transl_except(self) -> Optional[List[str]]: @@ -85,3 +174,7 @@ def transl_except(self) -> Optional[List[str]]: return None else: return self.cds_feature.qualifiers.get("transl_except") + + @property + def feature_seq(self): + return str(self._sr.seq) diff --git a/tests/test_filter_exonset_transcripts.py b/tests/test_filter_exonset_transcripts.py index 7ae9cf6..8bc7995 100644 --- a/tests/test_filter_exonset_transcripts.py +++ b/tests/test_filter_exonset_transcripts.py @@ -38,8 +38,8 @@ def test_filter_exonset(self, mock_logger): contents = f.read() self.assertEqual(contents, 'NR_046571.1\n') - mock_logger.warning.assert_called_with('Exon set transcript NR_046571.1 not found in txinfo file. Filtering out.') - mock_logger.info.assert_called_with('Filtered out exon sets for 1 transcript(s): NR_046571.1') + mock_logger.debug.assert_called_with('Exon set transcript NR_046571.1 not found in txinfo file. Filtering out.') + mock_logger.info.assert_called_with('Filtered out exon sets for 1 transcript(s)') if __name__ == "__main__": diff --git a/tests/test_uta_parsers_seqrecord.py b/tests/test_uta_parsers_seqrecord.py index b346cec..092ed63 100644 --- a/tests/test_uta_parsers_seqrecord.py +++ b/tests/test_uta_parsers_seqrecord.py @@ -17,8 +17,16 @@ class TestSeqRecordFacade(unittest.TestCase): file_name='rna.NM_001396027.gbff', expected_id='NM_001396027.1', expected_gene_symbol='FAM246C', + expected_gene_synonyms=[], + expected_gene_type="protein-coding", expected_gene_id='117134596', + expected_db_xrefs=["GeneID:117134596", "HGNC:HGNC:54842"], expected_cds_se_i=(0, 696), + expected_cds_product="protein FAM246C", + expected_cds_protein_id="NP_001382956.1", + expected_cds_translation="MAESGRPWAQARSAYRASEVLRRGTGRRRDPGPQSNGPGQEDARAPGRMARLRGQLRAEAASRSEVPRLLKLVERAGAG" \ + "AAGAGERTGAHSRGSVCSVCGEPRGGATYPAGVLEVSERRLQEGLAAVREELGAGIEALRAELRAELDALRALLPPPPSPPARREPRAVPRAAPRGPTLP" \ + "RTLGTVSALVAASRPADDAPDGPAECGAHRAPARKNHKKMPVPPGAPQGGGD", expected_exons_se_i=[(0, 696)], ), param( @@ -26,8 +34,21 @@ class TestSeqRecordFacade(unittest.TestCase): file_name='rna.NM_001996.gbff', expected_id='NM_001996.4', expected_gene_symbol='FBLN1', - expected_gene_id='2192', + expected_gene_synonyms=["FBLN","FIBL1"], + expected_gene_type="protein-coding", + expected_gene_id="2192", + expected_db_xrefs=["GeneID:2192", "HGNC:HGNC:3600", "MIM:135820"], expected_cds_se_i=(103, 2155), + expected_cds_product="fibulin-1 isoform C precursor", + expected_cds_protein_id="NP_001987.3", + expected_cds_translation="MERAAPSRRVPLPLLLLGGLALLAAGVDADVLLEACCADGHRMATHQKDCSLPYATESKECRMVQEQCCHSQLEELHCA" \ + "TGISLANEQDRCATPHGDNASLEATFVKRCCHCCLLGRAAQAQGQSCEYSLMVGYQCGQVFQACCVKSQETGDLDVGGLQETDKIIEVEEEQEDPYLNDR" \ + "CRGGGPCKQQCRDTGDEVVCSCFVGYQLLSDGVSCEDVNECITGSHSCRLGESCINTVGSFRCQRDSSCGTGYELTEDNSCKDIDECESGIHNCLPDFIC" \ + "QNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPIGHTCINTEGSYTCQKNVPNCGRGYHLNEEGTRCVDVDECAPPAEPCGKGHRCVNSPGS" \ + "FRCECKTGYYFDGISRMCVDVNECQRYPGRLCGHKCENTLGSYLCSCSVGFRLSVDGRSCEDINECSSSPCSQECANVYGSYQCYCRRGYQLSDVDGVTC" \ + "EDIDECALPTGGHICSYRCINIPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGGFRCLAFECPENYRRSAATRCERLPCHENREC" \ + "SKLPLRITYYHLSFPTNIQAPAVVFRMGPSSAVPGDSMQLAITGGNEEGFFTTRKVSPHSGVVALTKPVPEPRDLLLTVKMDLSRHGTVSSFVAKLFIFV" \ + "SAEL", expected_exons_se_i=[ (0, 182), (182, 288), @@ -51,18 +72,30 @@ class TestSeqRecordFacade(unittest.TestCase): file_name='rna.NR_173080.gbff', expected_id='NR_173080.1', expected_gene_symbol='LOC122455341', + expected_gene_synonyms=[], + expected_gene_type="ncRNA", expected_gene_id='122455341', + expected_db_xrefs=["GeneID:122455341"], expected_cds_se_i=None, - expected_exons_se_i=[(0,1073)], + expected_cds_product=None, + expected_cds_protein_id=None, + expected_cds_translation=None, + expected_exons_se_i=[], ), param( 'NR_173148 - no exon features, misc_RNA', file_name='rna.NR_173148.gbff', expected_id='NR_173148.1', expected_gene_symbol='FAM246C', + expected_gene_synonyms=[], + expected_gene_type="misc_RNA", expected_gene_id='117134596', + expected_db_xrefs=["GeneID:117134596", "HGNC:HGNC:54842"], expected_cds_se_i=None, - expected_exons_se_i=[(0,698)], + expected_cds_product=None, + expected_cds_protein_id=None, + expected_cds_translation=None, + expected_exons_se_i=[], ), ]) def test_seq_record_facade( @@ -71,8 +104,14 @@ def test_seq_record_facade( file_name, expected_id, expected_gene_symbol, + expected_gene_synonyms, + expected_gene_type, expected_gene_id, + expected_db_xrefs, expected_cds_se_i, + expected_cds_product, + expected_cds_protein_id, + expected_cds_translation, expected_exons_se_i, ): gbff_file = os.path.join(self.test_data_dir, file_name) @@ -80,19 +119,49 @@ def test_seq_record_facade( self.seq_record_facade = SeqRecordFacade(seq_record) assert self.seq_record_facade.id == expected_id assert self.seq_record_facade.gene_symbol == expected_gene_symbol + assert self.seq_record_facade.gene_synonyms == expected_gene_synonyms + assert self.seq_record_facade.gene_type == expected_gene_type assert self.seq_record_facade.gene_id == expected_gene_id + assert self.seq_record_facade.db_xrefs == expected_db_xrefs assert self.seq_record_facade.cds_se_i == expected_cds_se_i + assert self.seq_record_facade.cds_product == expected_cds_product + assert self.seq_record_facade.cds_protein_id == expected_cds_protein_id + assert self.seq_record_facade.cds_translation == expected_cds_translation assert self.seq_record_facade.exons_se_i == expected_exons_se_i + # @parameterized.expand([ @parameterized.expand([ + # param('no genes', features={}), + # param('no genes', features={'gene': []}), + # param('more than one gene', features={'gene': [Mock(), Mock()]}), + # param('more than one CDS', features={'CDS': [Mock(), Mock()]}), + # ]) + # def test_validate_features_by_type_invalid(self, test_name, features): + # with self.assertRaises(SeqRecordFeatureError): + # SeqRecordFacade.validate_features_by_type(features) + @parameterized.expand([ - param('no genes', features={}), - param('no genes', features={'gene': []}), - param('more than one gene', features={'gene': [Mock(), Mock()]}), - param('more than one CDS', features={'CDS': [Mock(), Mock()]}), + param("no gene feature", gene_feature_mock={}), + param("gene feature is None", gene_feature_mock={"gene": None}), + param("gene feature is empty", gene_feature_mock={"gene": []}), + param("gene feature has more than one", gene_feature_mock={"gene": [Mock(), Mock()]}), ]) - def test_validate_features_by_type_invalid(self, test_name, features): - with self.assertRaises(SeqRecordFeatureError): - SeqRecordFacade.validate_features_by_type(features) + def test_validate_gene_feature(self, test_name, gene_feature_mock): + with patch('uta.parsers.seqrecord.SeqRecordFacade.features_by_type', + new_callable=PropertyMock) as mock_features_by_type: + mock_features_by_type.return_value = gene_feature_mock + srf = SeqRecordFacade(seqrecord=Mock()) + with self.assertRaises(SeqRecordFeatureError): + _ = srf.gene_feature + + def test_cds_feature_validation_error(self): + with patch('uta.parsers.seqrecord.SeqRecordFacade.features_by_type', + new_callable=PropertyMock) as mock_features_by_type: + mock_cds_feature = Mock() + mock_cds_feature.qualifiers = {"protein_id": "NP_fake", "translation": "MNBVCXZ"} + mock_features_by_type.return_value = {'CDS': [mock_cds_feature, mock_cds_feature]} + srf = SeqRecordFacade(seqrecord=Mock()) + with self.assertRaises(SeqRecordFeatureError): + _ = srf.cds_feature def test_cds_feature(self): with patch('uta.parsers.seqrecord.SeqRecordFacade.features_by_type', new_callable=PropertyMock) as mock_features_by_type: @@ -100,11 +169,20 @@ def test_cds_feature(self): mock_features_by_type.return_value = {} srf = SeqRecordFacade(seqrecord=Mock()) self.assertIsNone(srf.cds_feature) + # one CDS feature - dummy_cds_feature = Mock() - mock_features_by_type.return_value = {'CDS': [dummy_cds_feature]} + desired_cds_feature = Mock() + desired_cds_feature.qualifiers = {"protein_id": "NP_fake", "translation": "MNBVCXZ"} + mock_features_by_type.return_value = {'CDS': [desired_cds_feature]} + srf = SeqRecordFacade(seqrecord=Mock()) + self.assertIs(srf.cds_feature, desired_cds_feature) + + # more than one CDS feature, but only one is returned + extra_cds_feature = Mock() + extra_cds_feature.qualifiers = {"other_key": "NP_fake", "second_key": "MNBVCXZ"} + mock_features_by_type.return_value = {'CDS': [desired_cds_feature, extra_cds_feature]} srf = SeqRecordFacade(seqrecord=Mock()) - self.assertIs(srf.cds_feature, dummy_cds_feature) + self.assertIs(srf.cds_feature, desired_cds_feature) def test_transl_except(self): with patch('uta.parsers.seqrecord.SeqRecordFacade.cds_feature', new_callable=PropertyMock) as mock_cds_feature: From 4cc66040bd1910b06fd0be954c9036d95f5bb023 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Wed, 8 May 2024 10:28:06 -0600 Subject: [PATCH 43/54] feat(IPVC-2407): check exon structure of exon alignment (#33) --- src/uta/loading.py | 76 +++++++++++---- tests/data/exonsets.mm-exons.gz | Bin 0 -> 220 bytes tests/test_uta_loading.py | 168 ++++++++++++++++++++++++++++++++ 3 files changed, 226 insertions(+), 18 deletions(-) create mode 100644 tests/data/exonsets.mm-exons.gz diff --git a/src/uta/loading.py b/src/uta/loading.py index 27187a8..3a93ad3 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -288,40 +288,80 @@ def load_exonset(session, opts, cf): update_period = 25 - session.execute(text("set role {admin_role};".format( - admin_role=cf.get("uta", "admin_role")))) + session.execute( + text("set role {admin_role};".format(admin_role=cf.get("uta", "admin_role"))) + ) session.execute(text("set search_path = " + usam.schema_name)) - n_rows = len(gzip.open(opts["FILE"], 'rt').readlines()) - 1 - esr = ufes.ExonSetReader(gzip.open(opts["FILE"], 'rt')) + n_rows = len(gzip.open(opts["FILE"], "rt").readlines()) - 1 + esr = ufes.ExonSetReader(gzip.open(opts["FILE"], "rt")) logger.info("opened " + opts["FILE"]) n_new = 0 n_unchanged = 0 n_deprecated = 0 + n_skipped = 0 n_errors = 0 for i_es, es in enumerate(esr): + skipped = False try: - n, o = _upsert_exon_set_record(session, es.tx_ac, es.alt_ac, es.strand, es.method, es.exons_se_i) + # determine if alignment and transcript have the same exon structure + tx_es = ( + session.query(usam.ExonSet) + .filter( + usam.ExonSet.tx_ac == es.tx_ac, + usam.ExonSet.alt_ac == es.tx_ac, + usam.ExonSet.alt_aln_method == "transcript", + ) + .one() + ) + tx_exon_count = len(tx_es.exons_se_i()) + aln_exon_count = len(es.exons_se_i.split(";")) + if tx_exon_count != aln_exon_count: + logger.warning( + "Exon structure mismatch: {tx_exon_count} exons in transcript {es.tx_ac}; {aln_exon_count} in alignment {es.alt_ac}".format( + tx_exon_count=tx_exon_count, + aln_exon_count=aln_exon_count, + es=es, + ) + ) + skipped = True + continue + + n, o = _upsert_exon_set_record( + session, es.tx_ac, es.alt_ac, es.strand, es.method, es.exons_se_i + ) session.commit() except IntegrityError as e: logger.exception(e) session.rollback() n_errors += 1 + else: + if skipped: + n_skipped += 1 + else: + (no) = (n is not None, o is not None) + if no == (True, False): + n_new += 1 + elif no == (True, True): + n_deprecated += 1 + elif no == (False, True): + n_unchanged += 1 finally: - (no) = (n is not None, o is not None) - if no == (True, False): - n_new += 1 - elif no == (True, True): - n_deprecated += 1 - elif no == (False, True): - n_unchanged += 1 - if i_es % update_period == 0 or i_es + 1 == n_rows: - logger.info("{i_es}/{n_rows} {p:.1f}%; {n_new} new, {n_unchanged} unchanged, {n_deprecated} deprecated, {n_errors} n_errors".format( - i_es=i_es, n_rows=n_rows, - n_new=n_new, n_unchanged=n_unchanged, n_deprecated=n_deprecated, n_errors=n_errors, - p=(i_es + 1) / n_rows * 100)) + logger.info( + "{i_es}/{n_rows} {p:.1f}%; {n_new} new, {n_unchanged} unchanged, {n_deprecated} deprecated, {n_skipped} skipped, {n_errors} n_errors".format( + i_es=i_es, + n_rows=n_rows, + n_new=n_new, + n_unchanged=n_unchanged, + n_deprecated=n_deprecated, + n_skipped=n_skipped, + n_errors=n_errors, + p=(i_es + 1) / n_rows * 100, + ) + ) + session.commit() @@ -727,7 +767,7 @@ def _fetch_origin_by_name(name): if no == (True, False): n_new += 1 elif no == (True, True): - logger.warn("Transcript {ti.ac} exon structure changed".format(ti=ti)) + logger.warning("Transcript {ti.ac} exon structure changed".format(ti=ti)) n_exons_changed += 1 elif no == (False, True): logger.debug("Transcript {ti.ac} exon structure unchanged".format(ti=ti)) diff --git a/tests/data/exonsets.mm-exons.gz b/tests/data/exonsets.mm-exons.gz new file mode 100644 index 0000000000000000000000000000000000000000..9cec37e1489650e65fbb16e7d469bd75a505a5cb GIT binary patch literal 220 zcmV<203-h&iwFpHcsgbP17&z`ZgXXHb1rRdEoFFbZgT*2k1=k;FbqX!>YMk^o&A4btNW{{yt$#_15I%hsQ-dHcK`!@B)U%Q4Ki>#~mP zJf3&^kC6yOQnVxv`*$DxAOT_hzMM}>K&Von(eIpxDgtws=4_N}Y!H<{5x17`ujtK9 zD!v}8R<}Jmq_re@Rp88MY!vSx!~ Date: Wed, 8 May 2024 10:28:34 -0600 Subject: [PATCH 44/54] feat(IPVC-2425): add migration to fix tx-alt-exon-pairs view (#34) --- ...f885cb84efce_update_tx_alt_exon_pairs_v.py | 57 +++++++++++++++++++ 1 file changed, 57 insertions(+) create mode 100644 src/alembic/versions/f885cb84efce_update_tx_alt_exon_pairs_v.py diff --git a/src/alembic/versions/f885cb84efce_update_tx_alt_exon_pairs_v.py b/src/alembic/versions/f885cb84efce_update_tx_alt_exon_pairs_v.py new file mode 100644 index 0000000..5ecaa9a --- /dev/null +++ b/src/alembic/versions/f885cb84efce_update_tx_alt_exon_pairs_v.py @@ -0,0 +1,57 @@ +"""update tx_alt_exon_pairs_v + +Revision ID: f885cb84efce +Revises: 14eed54ff90d +Create Date: 2024-05-07 21:01:03.693969 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa + + +# revision identifiers, used by Alembic. +revision: str = 'f885cb84efce' +down_revision: Union[str, None] = '14eed54ff90d' +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + op.execute("DROP VIEW IF EXISTS tx_alt_exon_pairs_v CASCADE;") + op.execute(""" + CREATE VIEW tx_alt_exon_pairs_v AS + SELECT g.symbol, g.symbol as hgnc, g.gene_id,TES.exon_SET_id AS tes_exon_SET_id, + AES.exon_SET_id AS aes_exon_SET_id, TES.tx_ac AS tx_ac,AES.alt_ac AS alt_ac, + AES.alt_strand,AES.alt_aln_method, TEX.ORD AS ORD,TEX.exon_id AS tx_exon_id, + AEX.exon_id AS alt_exon_id, TEX.start_i AS tx_start_i,TEX.END_i AS tx_END_i, + AEX.start_i AS alt_start_i, AEX.END_i AS alt_END_i, EA.exon_aln_id,EA.cigar + FROM exon_SET tes + JOIN transcript t ON tes.tx_ac=t.ac + JOIN gene g ON t.gene_id=g.gene_id + JOIN exon_set aes ON tes.tx_ac=aes.tx_ac AND tes.alt_aln_method='transcript' AND aes.alt_aln_method !~ 'transcript' + JOIN exon tex ON tes.exon_SET_id=tex.exon_SET_id + JOIN exon aex ON aes.exon_SET_id=aex.exon_SET_id AND tex.ORD=aex.ORD + LEFT JOIN exon_aln ea ON ea.tx_exon_id=tex.exon_id AND ea.alt_exon_id=AEX.exon_id; + """) + + +def downgrade() -> None: + op.execute("DROP VIEW IF EXISTS tx_alt_exon_pairs_v CASCADE;") + op.execute(""" + CREATE VIEW tx_alt_exon_pairs_v AS + SELECT g.symbol, g.symbol as hgnc, g.gene_id,TES.exon_SET_id AS tes_exon_SET_id, + AES.exon_SET_id AS aes_exon_SET_id, TES.tx_ac AS tx_ac,AES.alt_ac AS alt_ac, + AES.alt_strand,AES.alt_aln_method, TEX.ORD AS ORD,TEX.exon_id AS tx_exon_id, + AEX.exon_id AS alt_exon_id, TEX.start_i AS tx_start_i,TEX.END_i AS tx_END_i, + AEX.start_i AS alt_start_i, AEX.END_i AS alt_END_i, EA.exon_aln_id,EA.cigar + FROM exon_SET tes + JOIN transcript t ON tes.tx_ac=t.ac + JOIN gene g ON t.gene_id=g.gene_id + JOIN exon_set aes ON tes.tx_ac=aes.tx_ac AND tes.alt_aln_method='transcript' AND aes.alt_aln_method!='transcript' + JOIN exon tex ON tes.exon_SET_id=tex.exon_SET_id + JOIN exon aex ON aes.exon_SET_id=aex.exon_SET_id AND tex.ORD=aex.ORD + LEFT JOIN exon_aln ea ON ea.tx_exon_id=tex.exon_id AND ea.alt_exon_id=AEX.exon_id; + """) + From 3f970c8c028a6bfae788f4396513667cdc31860b Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Fri, 10 May 2024 16:47:51 -0600 Subject: [PATCH 45/54] feat(IPVC-2422): add migration to create materialized view for tx_exon_aln_v (#35) --- ...8_create_materialized_view_for_tx_exon_.py | 32 +++++++++++++++++++ 1 file changed, 32 insertions(+) create mode 100644 src/alembic/versions/19561fe444c8_create_materialized_view_for_tx_exon_.py diff --git a/src/alembic/versions/19561fe444c8_create_materialized_view_for_tx_exon_.py b/src/alembic/versions/19561fe444c8_create_materialized_view_for_tx_exon_.py new file mode 100644 index 0000000..ae21036 --- /dev/null +++ b/src/alembic/versions/19561fe444c8_create_materialized_view_for_tx_exon_.py @@ -0,0 +1,32 @@ +"""create materialized view for tx_exon_aln_v + +Revision ID: 19561fe444c8 +Revises: f885cb84efce +Create Date: 2024-05-07 21:59:09.078549 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa + + +# revision identifiers, used by Alembic. +revision: str = '19561fe444c8' +down_revision: Union[str, None] = 'f885cb84efce' +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + op.execute("DROP MATERIALIZED VIEW IF EXISTS tx_exon_aln_mv CASCADE;") + op.execute(""" + CREATE MATERIALIZED VIEW tx_exon_aln_mv AS SELECT * FROM tx_exon_aln_v WITH NO DATA; + CREATE INDEX tx_exon_aln_mv_tx_alt_ac_ix ON tx_exon_aln_mv(tx_ac, alt_ac, alt_aln_method); + GRANT SELECT ON tx_exon_set_summary_mv TO public; + REFRESH MATERIALIZED VIEW tx_exon_aln_mv; + """) + + +def downgrade() -> None: + op.execute("DROP MATERIALIZED VIEW IF EXISTS tx_exon_aln_mv CASCADE;") From 2f261e45660cb1e7c952c442898ea3496cd87856 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Fri, 10 May 2024 16:48:08 -0600 Subject: [PATCH 46/54] =?UTF-8?q?feat(IPVC-2435):=20add=20chromosome=20ref?= =?UTF-8?q?erence=20accession=20to=20unique=20alignme=E2=80=A6=20(#36)?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- sbin/ncbi_parse_genomic_gff.py | 6 +++- tests/test_ncbi_parse_genomic_gff.py | 53 +++++++++++++++++++++++++--- 2 files changed, 53 insertions(+), 6 deletions(-) diff --git a/sbin/ncbi_parse_genomic_gff.py b/sbin/ncbi_parse_genomic_gff.py index d784a10..0035d31 100755 --- a/sbin/ncbi_parse_genomic_gff.py +++ b/sbin/ncbi_parse_genomic_gff.py @@ -45,6 +45,10 @@ class GFFRecord: parent_id: str transcript_id: str + @property + def key(self) -> str: + return f"{self.transcript_id}:{self.seqid}" + def _sort_exons(exons: List[GFFRecord]) -> List[GFFRecord]: return sorted(exons, key=lambda e: e.exon_number) @@ -115,7 +119,7 @@ def parse_gff_files(file_paths: List[str]) -> dict[str, List[GFFRecord]]: except ValueError as e: raise Exception(f"Failed at line :{line} with error: {e}") if record: - tx_data[record.parent_id].append(record) + tx_data[record.key].append(record) return {k: _sort_exons(v) for k, v in tx_data.items()} diff --git a/tests/test_ncbi_parse_genomic_gff.py b/tests/test_ncbi_parse_genomic_gff.py index b40edeb..4a0b638 100644 --- a/tests/test_ncbi_parse_genomic_gff.py +++ b/tests/test_ncbi_parse_genomic_gff.py @@ -44,6 +44,15 @@ def setUp(self): f.write( "NC_000001.10\tBestRefSeq\texon\t13221\t14409\t.\t+\t.\tID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n" ) + f.write( + "NC_000001.11\tBestRefSeq\texon\t15874\t16227\t.\t+\t.\tID=exon-NR_046018.2-1;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n" + ) + f.write( + "NC_000001.11\tBestRefSeq\texon\t16613\t16721\t.\t+\t.\tID=exon-NR_046018.2-2;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n" + ) + f.write( + "NC_000001.11\tBestRefSeq\texon\t17221\t18409\t.\t+\t.\tID=exon-NR_046018.2-3;Parent=rna-NR_046018.2;transcript_id=NR_046018.2\n" + ) temp_gff.seek(0) self.temp_gff = temp_gff self.gff_records = [ @@ -74,6 +83,33 @@ def setUp(self): parent_id="rna-NR_046018.2", transcript_id="NR_046018.2", ), + GFFRecord( + seqid="NC_000001.11", + start=15874, + end=16227, + strand="+", + exon_number=1, + parent_id="rna-NR_046018.2", + transcript_id="NR_046018.2", + ), + GFFRecord( + seqid="NC_000001.11", + start=16613, + end=16721, + strand="+", + exon_number=2, + parent_id="rna-NR_046018.2", + transcript_id="NR_046018.2", + ), + GFFRecord( + seqid="NC_000001.11", + start=17221, + end=18409, + strand="+", + exon_number=3, + parent_id="rna-NR_046018.2", + transcript_id="NR_046018.2", + ), ] def tearDown(self): @@ -93,6 +129,7 @@ def test_parse_gff_record(self): ) parsed_record = parse_gff_record(line) self.assertEqual(parsed_record, expected_record) + self.assertEqual(parsed_record.key, f"{expected_record.transcript_id}:{expected_record.seqid}") def test_parse_gff_record_skips_non_exon_records(self): # We exclude non-exon records @@ -102,7 +139,7 @@ def test_parse_gff_record_skips_non_exon_records(self): self.assertEqual(parsed_record, expected_record) def test_parse_gff_record_skips_missing_transcript_id(self): - # We exclude alignments missing transcript_id + # We exclude alignments missing a parent field line = sample_line( attributes_str="ID=exon-NR_046018.2-1;transcript_id=NR_046018.2" ) # parent missing from attributes @@ -111,7 +148,7 @@ def test_parse_gff_record_skips_missing_transcript_id(self): self.assertEqual(parsed_record, expected_record) def test_parse_gff_record_skips_missing_parent_field(self): - # We exclude alignments missing a parent field + # We exclude alignments missing transcript_id line = sample_line( attributes_str="ID=exon-NR_046018.2-1;Parent=rna-NR_046018.2" ) # transcript_id missing from attributes @@ -159,7 +196,10 @@ def test_parse_gff_record_raises_unparseable_id(self): def test_parse_gff_file(self): # Test parsing the entire uncompressed GFF file - expected_result = {"rna-NR_046018.2": self.gff_records} + expected_result = { + "NR_046018.2:NC_000001.10": self.gff_records[:3], + "NR_046018.2:NC_000001.11": self.gff_records[3:], + } parsed_result = parse_gff_files([self.temp_gff.name]) self.assertEqual(parsed_result, expected_result) @@ -170,13 +210,16 @@ def test_parse_gff_file_accepts_gzipped_files(self): f_out.write(f_in.read()) # Test parsing the gzipped GFF file - expected_result = {"rna-NR_046018.2": self.gff_records} + expected_result = { + "NR_046018.2:NC_000001.10": self.gff_records[:3], + "NR_046018.2:NC_000001.11": self.gff_records[3:], + } parsed_result = parse_gff_files([self.temp_gff.name + ".gz"]) self.assertEqual(parsed_result, expected_result) def test_get_zero_based_exon_ranges(self): # Test converting exon ranges to 0-based half-open format yields expected values - exon_ranges = get_zero_based_exon_ranges(self.gff_records) + exon_ranges = get_zero_based_exon_ranges(self.gff_records[:3]) assert exon_ranges == "11873,12227;12612,12721;13220,14409" def test_script_output(self): From 59fd1b2e4737248d5bd113c3cb30de08f3062604 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Tue, 14 May 2024 15:39:57 -0600 Subject: [PATCH 47/54] feat(IPVC-2438): coalesce cdna match alignments (#37) --- sbin/coalesce_exonsets.py | 57 ++++++++ {archive => sbin}/ncbi-parse-gff | 230 +++++++++---------------------- sbin/uta-extract | 15 +- tests/test_coalesce_exonsets.py | 48 +++++++ 4 files changed, 186 insertions(+), 164 deletions(-) create mode 100755 sbin/coalesce_exonsets.py rename {archive => sbin}/ncbi-parse-gff (54%) create mode 100644 tests/test_coalesce_exonsets.py diff --git a/sbin/coalesce_exonsets.py b/sbin/coalesce_exonsets.py new file mode 100755 index 0000000..2ba0df8 --- /dev/null +++ b/sbin/coalesce_exonsets.py @@ -0,0 +1,57 @@ +#!/usr/bin/env python + +""" +This script coalesces exonsets from multiple input files. It builds a cache of tx_ac/alt_ac pairs. If a mapping is +seen in a later input file, the exonset is skipped. The output is written to stdout. +""" + +import argparse +import logging.config +import sys +from typing import Dict, List, Tuple + +import importlib_resources + +from uta.formats.exonset import ExonSetReader, ExonSetWriter +from uta.tools.file_utils import open_file + +logging_conf_fn = importlib_resources.files("uta").joinpath("etc/logging.conf") +logging.config.fileConfig(logging_conf_fn) +logging.getLogger().setLevel(logging.INFO) +logger = logging.getLogger(__name__) + + +def coalesce_exonsets(exonset_files: List[str]) -> None: + skipped = 0 + esw = ExonSetWriter(sys.stdout) + seen_ess: Dict[Tuple[str, str], str] = {} + + for exonset_fn in exonset_files: + logger.info(f" - processing exonset file {exonset_fn}") + with open_file(exonset_fn) as f: + exonsets = ExonSetReader(f) + for exonset in exonsets: + key = (exonset.tx_ac, exonset.alt_ac) + if key in seen_ess: + logger.warning(f" - exon set for transcript {exonset.tx_ac}/{exonset.alt_ac} already " + f"seen in {seen_ess[(exonset.tx_ac, exonset.alt_ac)]}. Skipping.") + skipped += 1 + else: + seen_ess[key] = exonset_fn + esw.write(exonset) + + logger.info(f"Coalesced {len(seen_ess)} exonsets from {len(exonset_files)} files, skipped {skipped} duplicates.") + return seen_ess + + +def main(): + parser = argparse.ArgumentParser(description='Coalesce exonsets.') + parser.add_argument('exonsets', nargs="+", help='Path to the exonset file') + args = parser.parse_args() + + logger.info(f"Coalescing exonsets from {len(args.exonsets)} files") + coalesce_exonsets(args.exonsets) + + +if __name__ == '__main__': + main() diff --git a/archive/ncbi-parse-gff b/sbin/ncbi-parse-gff similarity index 54% rename from archive/ncbi-parse-gff rename to sbin/ncbi-parse-gff index 4a34ed2..cdc546a 100755 --- a/archive/ncbi-parse-gff +++ b/sbin/ncbi-parse-gff @@ -1,17 +1,13 @@ #!/usr/bin/env python -"""Write exonsets and txinfo files from NCBI GFF alignments, as obtained from -ftp://ftp.ncbi.nlm.nih.gov/refseq/H_sapiens/alignments/ +"""Write exonsets files from NCBI GFF alignments, as obtained from +ftp://ftp.ncbi.nlm.nih.gov/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_*/ This service appeared in April 2015 and is due to update weekly. See uta.formats for a description of those file formats. In a nutshell, this means that you'll get data like this: -ncbi.txinfo.gz: -origin ac hgnc cds_se_i exons_se_i -NCBI RefSeq NM_053283.2 DCD 62,395 0,120;120,159;159,261;261,351;351,517 - ncbi.exonsets.gz: tx_ac alt_ac method strand exons_se_i NM_130786.3 NC_000019.9 splign -1 58864769,58864865;588646... @@ -35,12 +31,12 @@ from __future__ import division import argparse import collections import gzip +import importlib_resources import io import itertools import logging.config import os import pprint -import pkg_resources import re import sys @@ -50,6 +46,7 @@ import prettytable from uta.formats.exonset import ExonSet, ExonSetWriter from uta.formats.txinfo import TxInfo, TxInfoWriter, TxInfoReader from uta.formats.geneaccessions import GeneAccessionsReader +from uta.tools.file_utils import open_file origin = "NCBI" @@ -138,23 +135,22 @@ class TranscriptAlignment(object): return self.exon_alignments[0].pct_identity_ungap -def parse_args(argv): +def parse_args(): ap = argparse.ArgumentParser( description=__doc__, ) - ap.add_argument("in_fn") + ap.add_argument("GFF_files", nargs="+", + help="NCBI GFF files to process") ap.add_argument("--origin", "-o", default=origin) ap.add_argument("--prefix", "-p", default="ncbi-gff") - ap.add_argument("--geneacs", "-G") - ap.add_argument("--txinfo", "-T", required=False) ap.add_argument("--strict-coverage", "-C", type=float, default=95.0) ap.add_argument("--min-coverage", "-c", type=float, default=85.0) ap.add_argument("--strict-pct-identity-gap", "-I", type=float, default=95.0) ap.add_argument("--min-pct-identity-gap", "-i", type=float, default=85.0) - opts = ap.parse_args(argv) + opts = ap.parse_args() assert opts.strict_coverage > opts.min_coverage assert opts.strict_pct_identity_gap > opts.min_pct_identity_gap @@ -165,24 +161,28 @@ def parse_args(argv): def read_exon_alignments(fn): """read lines of NCBI's alignment gff file, fn, returning ExonAlignment records""" - # NC_000007.13 RefSeq cDNA_match 50344265 50344518 254 + . ID=aln58042;Target=NM_001220765.2 1 254 +;gap_count=0;identity=0.0691326;idty=1;num_ident=428;num_mismatch=0;pct_coverage=6.91326;pct_identity_gap=100;pct_identity_ungap=100;score=254 - # NC_000002.11 RefSeq cDNA_match 179671939 179672150 212 - . ID=ed951d46-194c-477a-a480-4bc64530c5ba;Target=NM_001267550.2 1 212 +;gap_count=0;identity=0.999991;idty=1;num_ident=109223;num_mismatch=1;pct_coverage=100;pct_identity_gap=99.9991;pct_identity_ungap=99.9991 + # NC_000022.10 RefSeq cDNA_match 20783512 20783627 116 - . ID=7b8c7a437b92bf9dee20d81acadadd8e;Target=NM_182895.5 1496 1611 +;consensus_splices=20;exon_identity=0.99769;for_remapping=2;gap_count=3;identity=0.99769;idty=1;matches=3455;num_ident=3455;num_mismatch=5;pct_coverage=99.9134;pct_coverage_hiqual=99.9134;pct_identity_gap=99.769;pct_identity_ungap=99.8555;product_coverage=1;rank=1;splices=20;weighted_identity=0.996898 + # NC_000022.10 RefSeq cDNA_match 20781685 20781837 153 - . ID=7b8c7a437b92bf9dee20d81acadadd8e;Target=NM_182895.5 1612 1764 +;consensus_splices=20;exon_identity=0.99769;for_remapping=2;gap_count=3;identity=0.99769;idty=1;matches=3455;num_ident=3455;num_mismatch=5;pct_coverage=99.9134;pct_coverage_hiqual=99.9134;pct_identity_gap=99.769;pct_identity_ungap=99.8555;product_coverage=1;rank=1;splices=20;weighted_identity=0.996898 + # NC_000022.10 RefSeq cDNA_match 20778874 20780569 1676.05 - . ID=7b8c7a437b92bf9dee20d81acadadd8e;Target=NM_182895.5 1765 3463 +;consensus_splices=20;exon_identity=0.99769;for_remapping=2;gap_count=3;identity=0.99769;idty=0.995291;matches=3455;num_ident=3455;num_mismatch=5;pct_coverage=99.9134;pct_coverage_hiqual=99.9134;pct_identity_gap=99.769;pct_identity_ungap=99.8555;product_coverage=1;rank=1;splices=20;weighted_identity=0.996898;Gap=M540 I1 M5 I1 M51 I1 M1100 line_re = re.compile( "(?P\S+)\s+(?P\S+)\s+(?P\S+)\s+" "(?P\d+)\s+(?P\d+)\s+(?P\S+)\s+" "(?P[-+])\s+\.\s+ID=(?P[^;]+);Target=(?P\S+)" "\s+(?P\d+)\s+(?P\d+).+?" - "pct_coverage=(?P[^;]+);" + "pct_coverage=(?P[^;]+);.+?" "pct_identity_gap=(?P[^;]+);" "pct_identity_ungap=(?P[^;]+)" ) - fh = io.open(fn, "rt") - for line in fh.readlines(): - if not line.startswith('#'): - try: - yield ExonAlignment(**line_re.match(line).groupdict()) - except (AttributeError, ValueError): - raise Exception("Failed at", line) + + with open_file(fn) as fh: + for line in fh: + if not line.startswith('#'): + try: + re_match = line_re.match(line) + if re_match and re_match["match_type"] == "cDNA_match": + yield ExonAlignment(**line_re.match(line).groupdict()) + except (AttributeError, ValueError): + raise Exception("Failed at", line) def read_transcript_alignments(fn): @@ -217,14 +217,8 @@ def group_transcript_alignments(transcript_alignments): for key, alns_i in itertools.groupby(transcript_alignments, key=_key)) -def convert_exon_data(opts, transcript_alignment): +def convert_exon_data(transcript_alignment): """return (TxInfo,ExonSet) tuple for given exon record data""" - ti = TxInfo(ac=transcript_alignment.tx_ac, - origin=opts.origin, - hgnc=None, - cds_se_i=None, - exons_se_i=transcript_alignment.tx_exons_se_i - ) es = ExonSet( tx_ac=transcript_alignment.tx_ac, alt_ac=transcript_alignment.ref_ac, @@ -232,67 +226,20 @@ def convert_exon_data(opts, transcript_alignment): strand=-1 if transcript_alignment.strand == "-" else 1, exons_se_i=transcript_alignment.ref_exons_se_i ) - return (ti, es) - + return es -if __name__ == "__main__": - logging_conf_fn = pkg_resources.resource_filename( - "uta", "etc/logging.conf") - logging.config.fileConfig(logging_conf_fn) - logging.getLogger().setLevel(logging.INFO) - logger = logging.getLogger(__name__) - - opts = parse_args(sys.argv[1:]) - - if opts.geneacs: - gar = GeneAccessionsReader(gzip.open(opts.geneacs, "rt")) - tx2gene = {ga.tx_ac: ga.hgnc for ga in gar} - logger.info( - "read {} gene-accession mappings from {}".format(len(tx2gene), opts.geneacs)) - else: - tx2gene = None - logger.info("No geneacs (-G) file provided; gene info will be empty.") - - if opts.txinfo: - tir = TxInfoReader(gzip.open(opts.txinfo, "rt")) - tx2ti = {ti.ac: ti for ti in tir} - logger.info( - "read {} CDS data from {}".format(len(tx2ti), opts.txinfo)) - # add any gene-accession mappings from txinfo file if they are not in geneacs file; log warning if they disagree - if tx2gene: - for ti_ac in tx2ti: - if not tx2gene.get(ti_ac): - tx2gene[ti_ac] = tx2ti[ti_ac].hgnc - if tx2gene[ti_ac] != tx2ti[ti_ac].hgnc: - logger.warning('HGNC symbol disagrees in txinfo ({tx2ti_hgnc}) and geneacs ({tx2gene_hgnc}) files for accession {ti_ac}'.format( - tx2ti_hgnc=tx2ti[ti_ac].hgnc, - tx2gene_hgnc=tx2gene[ti_ac].hgnc, - ti_ac=ti_ac - )) - else: - tx2ti = None - logger.info("No gbff txinfo provided (-T); CDS start,end will be undefined for all transcripts and transcript-genome exon structures will not be verified") - - es_fn = opts.prefix + "exonset.gz" - ti_fn = opts.prefix + "txinfo.gz" - - esw = ExonSetWriter(gzip.open(es_fn + ".tmp", "wt")) - tiw = TxInfoWriter(gzip.open(ti_fn + ".tmp", "wt")) - - ties = {} - ti_written = collections.defaultdict(lambda: False) - ac_not_in_gbff = set() - ac_exons_differ = set() +def write_exonsets_from_gff_file(gff_fn, logger, opts, esw): + """write exonsets from a single gff file""" ac_in_source = set() ac_failed = set() - bins = "nogbff esdiffer unique multiple minimum none".split() + bins = "unique multiple minimum none skipped".split() sets = collections.defaultdict(lambda: {k: list() for k in bins}) - transcript_alignments = read_transcript_alignments(opts.in_fn) + transcript_alignments = read_transcript_alignments(gff_fn) logger.info( - "read {} transcript alignments from {}".format(len(transcript_alignments), opts.in_fn)) + "read {} transcript alignments from {}".format(len(transcript_alignments), gff_fn)) for _, txalns in group_transcript_alignments(transcript_alignments): assert len(txalns) > 0 @@ -300,40 +247,13 @@ if __name__ == "__main__": ta0 = txalns[0] tx_ac, ref_ac = ta0.tx_ac, ta0.ref_ac skey = "{:.2s} {:.2s}".format(tx_ac, ref_ac) + if not tx_ac[:2] in ("NM", "NR") or not ref_ac[:2] == "NC": + sets[skey]["skipped"] += [txalns] + continue + bin = None - - # ############################################################ - # Optionally compare exon structure from gbff with input gff - # And get cds s,e from gbff (sigh) - if tx2ti is None: - cds_se_i = None - txalns_esm = txalns - else: - if tx_ac not in tx2ti: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: no transcript info in {opts.txinfo}; skipping transcript".format( - ta=ta0, opts=opts)) - ac_not_in_gbff.add(tx_ac) - bin = "nogbff" - sets[skey][bin] += [txalns] - continue - - gbff_ti = tx2ti[tx_ac] - txalns_esm = [ta for ta in txalns if ta.tx_exons_se_i == gbff_ti.exons_se_i] - n_rm = len(txalns) - len(txalns_esm) - if n_rm > 0: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: Removed {n_rm}/{n_tot} exon structures that differ from gbff definition".format( - n_rm=n_rm, n_tot=len(txalns), ta=ta, opts=opts)) - if len(txalns_esm) == 0: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: All {n} exon structures differ from gbff definition; skipping alignment".format( - ta=ta, opts=opts, n=len(txalns))) - ac_exons_differ.add(tx_ac) - bin = "esdiffer" - sets[skey][bin] += [txalns] - continue - - cds_se_i = gbff_ti.cds_se_i # possibly None - - + txalns_load = [] + # ############################################################ # Filter alignments by coverage and pct_identity_gap # From Terence Murphy, NCBI: @@ -341,7 +261,7 @@ if __name__ == "__main__": # and RefSeqGene alignments that meet the filter: # 'pct_identity_gap >= 99.5 and pct_coverage >= 95'" txalns_strict = [txaln - for txaln in txalns_esm + for txaln in txalns if (txaln.pct_coverage > opts.strict_coverage and txaln.pct_identity_gap > opts.strict_pct_identity_gap)] @@ -354,90 +274,76 @@ if __name__ == "__main__": logger.warning("{ta.tx_ac}~{ta.ref_ac}: Multiple ({n}) strict alignments; cov/pig: {stats}".format( ta=txalns_strict[0], n=len(txalns_strict), opts=opts, - stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_strict), - )) + stats="; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_strict), + )) txalns_load = txalns_strict bin = "multiple" if len(txalns_strict) == 0: txalns_min = [txaln - for txaln in txalns_esm + for txaln in txalns if (txaln.pct_coverage > opts.min_coverage and txaln.pct_identity_gap > opts.min_pct_identity_gap)] if len(txalns_min) == 0: logger.warning("{ta.tx_ac}~{ta.ref_ac}: No usable alignments; cov/pig: {stats}".format( ta=txalns[0], - stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_esm), - )) + stats="; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns), + )) bin = "none" + ac_failed.add(skey) else: - logger.warning("{ta.tx_ac}~{ta.ref_ac}: Resorting to minimum criteria; loading {n} alignments; cov/pig: {stats}".format( - ta=txalns_min[0], n=len(txalns_min), - stats = "; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_min), + logger.warning( + "{ta.tx_ac}~{ta.ref_ac}: Resorting to minimum criteria; loading {n} alignments; cov/pig: {stats}".format( + ta=txalns_min[0], n=len(txalns_min), + stats="; ".join("{ta.pct_coverage}/{ta.pct_identity_gap}".format(ta=ta) for ta in txalns_min), )) bin = "minimum" txalns_load = txalns_min - sets[skey][bin] += [txalns_esm] + sets[skey][bin] += [txalns] for ta in txalns_load: - ti, es = convert_exon_data(opts, ta) - ti.cds_se_i = cds_se_i + es = convert_exon_data(ta) ac_in_source.add(tx_ac) - ti.hgnc = tx2gene.get(ti.ac, None) - - if not ti_written[ti.ac]: - # write a single txinfo line once; multiple may occur for multiple alignments of e.g., one NM to NC, NW, NT - tiw.write(ti) - ti_written[ti.ac] = True esw.write(es) # END HEINOUS LOOP - for fn in [ti_fn, es_fn]: - os.rename(fn + ".tmp", fn) - seen_but_failed = ac_failed - ac_in_source if seen_but_failed: logger.warning("{n_acv} acvs seen but failed criteria: {acs}".format( n_acv=len(seen_but_failed), acs=",".join(sorted(seen_but_failed)))) - if ac_not_in_gbff: - s_not_g_b = set(k.partition(".")[ - 0] for k in ac_in_source) - set(k.partition(".")[0] for k in tx2gene.keys()) - logger.warning("{n_acv} acvs ({n_ac} base acs) in source not in geneacs file: {acs}".format( - n_acv=len(ac_not_in_gbff), n_ac=len(s_not_g_b), opts=opts, acs=",".join(sorted(ac_not_in_gbff)))) - - if ac_exons_differ: - logger.warning("{n} accessions in gbff-derived txinfo have different exon coordinates: {acs}".format( - n=len(ac_exons_differ), opts=opts, acs=",".join(sorted(ac_exons_differ)))) - - pprint.pprint(opts) pt = prettytable.PrettyTable(field_names=["ac_pair"] - + bins - + "max_coverage max_pct_identity_gap nobgffs nogbff_noup esdiffers nones".split() + + bins + + "max_coverage max_pct_identity_gap nones".split() ) for ack in sorted(sets.keys()): n = 5 - nogbff_acs = sorted(set(ta.tx_ac for ta in itertools.chain.from_iterable(sets[ack]["nogbff"])))[:n] - esdiffer_acs = sorted(set(ta.tx_ac for ta in itertools.chain.from_iterable(sets[ack]["esdiffer"])))[:n] nones = list(itertools.chain.from_iterable(sets[ack]["none"])) nones_acs = sorted(set(ta.tx_ac for ta in nones))[:n] max_pct_identity_gap = "{:.2f}".format(max(ta.pct_identity_gap for ta in nones)) if nones else "n/a" max_pct_coverage = "{:.2f}".format(max(ta.pct_coverage for ta in nones)) if nones else "n/a" - nogbff_noup = sorted( - set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["nogbff"])) - - set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["unique"])) - - set(ta.tx_ac.split('.')[0] for ta in itertools.chain.from_iterable(sets[ack]["multiple"])) - ) - pt.add_row([ack] + [len(sets[ack][bk]) for bk in bins] + [max_pct_coverage, max_pct_identity_gap, - " ".join(nogbff_acs), - str(len(nogbff_noup)) + ": " + " ".join(nogbff_noup[:n]), - " ".join(esdiffer_acs), - " ".join(nones_acs) ]) - print(pt) + " ".join(nones_acs)]) + logger.info("summary in table below...\n" + str(pt)) + + + +if __name__ == "__main__": + logging_conf_fn = importlib_resources.files("uta").joinpath("etc/logging.conf") + logging.config.fileConfig(logging_conf_fn) + logging.getLogger().setLevel(logging.INFO) + logger = logging.getLogger(__name__) + + opts = parse_args() + + esw = ExonSetWriter(sys.stdout) + + for gff_fn in opts.GFF_files: + logger.info("processing {}".format(gff_fn)) + write_exonsets_from_gff_file(gff_fn, logger, opts, esw) diff --git a/sbin/uta-extract b/sbin/uta-extract index c4e239d..5d3f072 100755 --- a/sbin/uta-extract +++ b/sbin/uta-extract @@ -28,10 +28,21 @@ sbin/ncbi-parse-gbff "$GBFF_files" | gzip -c > "$working_dir/txinfo.gz" 2>&1 | \ tee "$log_dir/ncbi-parse-gbff.log" # parse alignments from GFF input files -GFF_files=$(ls $ncbi_dir/genomes/refseq/vertebrate_mammalian/Homo_sapiens/all_assembly_versions/GCF_000001405*/GCF_*_genomic.gff.gz) -sbin/ncbi_parse_genomic_gff.py "$GFF_files" | gzip -c > "$working_dir/unfiltered_exonsets.gz" 2>&1 | \ +# Due to NCBI's handling of transcripts with "frameshifting insertions and deletions with micro-introns" we +# need to parse out the cDNA_match alignment and use them preferentially over exons from genome GFF files. +# The cDNA_match records include the indels and do not have micro-introns. +mapfile -t GFF_FILES < <(find "$ncbi_dir/genomes" -type f -name "GCF_*_genomic.gff.gz") +sbin/ncbi-parse-gff "${GFF_FILES[@]}" | gzip -c > "$working_dir/cdna_match.exonsets.gz" 2>&1 | \ + tee "$log_dir/ncbi_parse_gff.log" + +# extract exon blocks from GFF files +sbin/ncbi_parse_genomic_gff.py "${GFF_FILES[@]}" | gzip -c > "$working_dir/exon_block.exonsets.gz" 2>&1 | \ tee "$log_dir/ncbi-parse-genomic-gff.log" +# coalesce exonsets +sbin/coalesce_exonsets.py "$working_dir/cdna_match.exonsets.gz" "$working_dir/exon_block.exonsets.gz" | \ + gzip -c > "$working_dir/unfiltered_exonsets.gz" 2>&1 | tee "$log_dir/coalesce_exonsets.log" + # filter transcripts sbin/filter_exonset_transcripts.py --tx-info "$working_dir/txinfo.gz" --exonsets "$working_dir/unfiltered_exonsets.gz" \ --missing-ids "$working_dir/filtered_tx_acs.txt" | gzip -c > "$working_dir/exonsets.gz" 2>&1 | \ diff --git a/tests/test_coalesce_exonsets.py b/tests/test_coalesce_exonsets.py new file mode 100644 index 0000000..757ec0b --- /dev/null +++ b/tests/test_coalesce_exonsets.py @@ -0,0 +1,48 @@ +import contextlib +import io +import sys +import unittest +from tempfile import NamedTemporaryFile +from unittest.mock import patch + +from sbin.coalesce_exonsets import coalesce_exonsets +from uta.formats.exonset import ExonSetWriter + + +class TestCoalesceExonsets(unittest.TestCase): + + def _create_temporary_file(self, lines): + with NamedTemporaryFile(delete=False) as temp_exonsets: + with open(temp_exonsets.name, "wt") as f: + for line in lines: + f.write(line) + temp_exonsets.seek(0) + return temp_exonsets.name + + @patch('sbin.coalesce_exonsets.logger') + def test_coalesce_exonsets(self, mock_logger): + lines_1 = [ + "tx_ac\talt_ac\tmethod\tstrand\texons_se_i\n", + "NM_145660.2\tNC_000022.10\tsplign\t-1\t36600673,36600879;36598038,36598101;36595375,36595422;36591356,36591483;36585175,36587958\n", + "NM_000348.4\tNC_000002.11\tsplign\t-1\t31805689,31806007;31758672,31758836;31756440,31756542;31754376,31754527;31747549,31751332\n" + ] + lines_2 = [ + "tx_ac\talt_ac\tmethod\tstrand\texons_se_i\n", + "NM_145660.2\tNC_000022.10\tsplign\t-1\t36600673,36600879;36598038,36598101;36595375,36595422;36591356,36591483;36587846,36587958;36585175,36587845\n", + "NM_145660.2\tNC_000022.11\tsplign\t-1\t36204627,36204833;36201992,36202055;36199329,36199376;36195310,36195437;36189127,36191912\n", + "NM_001005484.2\tNC_000001.10\tsplign\t1\t65418,65433;65519,65573;69036,71585\n" + ] + temp_exonsets_1_fn = self._create_temporary_file(lines_1) + temp_exonsets_2_fn = self._create_temporary_file(lines_2) + + # the first record in lines_2 (NM_145660.2, NC_000022.10) will be skipped, as it is already passed to the output + expected_output = lines_1 + lines_2[2:] + stdout = io.StringIO() + + with contextlib.redirect_stdout(stdout): + coalesce_exonsets([temp_exonsets_1_fn, temp_exonsets_2_fn]) + + output = stdout.getvalue() + self.assertEqual(output, ''.join(expected_output)) + + mock_logger.warning.assert_called_with(f" - exon set for transcript NM_145660.2/NC_000022.10 already seen in {temp_exonsets_1_fn}. Skipping.") From c0d5a376940413522f319a33409b558b4a30c4a2 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Wed, 15 May 2024 08:57:32 -0600 Subject: [PATCH 48/54] feat(IPVC-2441): various workflow fixes (#39) --- etc/ncbi-files.txt | 2 +- sbin/seqrepo-load | 4 ++-- sbin/uta-extract | 8 ++++---- src/uta/exceptions.py | 4 ++++ src/uta/loading.py | 43 ++++++++++++++++++++++++------------------- 5 files changed, 35 insertions(+), 26 deletions(-) diff --git a/etc/ncbi-files.txt b/etc/ncbi-files.txt index 2b9bfb6..0a70efc 100644 --- a/etc/ncbi-files.txt +++ b/etc/ncbi-files.txt @@ -5,7 +5,7 @@ # │ ├── GENE_INFO # │ │ └── Mammalia # │ │ └── Homo_sapiens.gene_info.gz -# │ └── gene2accession.gz +# │ └── gene2refseq.gz # ├── genomes # │ └── refseq # │ └── vertebrate_mammalian diff --git a/sbin/seqrepo-load b/sbin/seqrepo-load index 3eb3345..02197ec 100755 --- a/sbin/seqrepo-load +++ b/sbin/seqrepo-load @@ -1,6 +1,6 @@ #!/usr/bin/env bash -set -e +set -euxo pipefail seqrepo_root=$1 seqrepo_version=$2 @@ -19,5 +19,5 @@ mapfile -t FASTA_FILES < <(find "$sequence_dir" -type f -name "*.f[an]a*") # Load SeqRepo with new sequences seqrepo --root-directory "$seqrepo_root" \ load -n NCBI --instance-name "$seqrepo_version" \ - "${FASTA_FILES[@]}" 2>& 1 | \ + "${FASTA_FILES[@]}" 2>&1 | \ tee "$log_dir/seqrepo-load.log" diff --git a/sbin/uta-extract b/sbin/uta-extract index 5d3f072..9dbf716 100755 --- a/sbin/uta-extract +++ b/sbin/uta-extract @@ -2,7 +2,7 @@ # Extract data from NCBI files into intermediate files. -set -e +set -euxo pipefail ncbi_dir=$1 working_dir=$2 @@ -19,12 +19,12 @@ sbin/ncbi-parse-geneinfo $ncbi_dir/gene/DATA/GENE_INFO/Mammalia/Homo_sapiens.gen gzip -c > "$working_dir/geneinfo.gz" 2>&1 | tee "$log_dir/ncbi-parse-geneinfo.log" # transcript protein associations -sbin/ncbi-parse-gene2refseq $ncbi_dir/gene/DATA/gene2accession.gz | gzip -c > "$working_dir/assocacs.gz" 2>&1 | \ +sbin/ncbi-parse-gene2refseq $ncbi_dir/gene/DATA/gene2refseq.gz | gzip -c > "$working_dir/assocacs.gz" 2>&1 | \ tee "$log_dir/ncbi-fetch-assoc-acs.log" # parse transcript info from GBFF input files -GBFF_files=$(ls $ncbi_dir/refseq/H_sapiens/mRNA_Prot/human.*.rna.gbff.gz) -sbin/ncbi-parse-gbff "$GBFF_files" | gzip -c > "$working_dir/txinfo.gz" 2>&1 | \ +mapfile -t GBFF_FILES < <(find "$ncbi_dir/refseq" -type f -name "human.*.rna.gbff.gz") +sbin/ncbi-parse-gbff "${GBFF_FILES[@]}" | gzip -c > "$working_dir/txinfo.gz" 2>&1 | \ tee "$log_dir/ncbi-parse-gbff.log" # parse alignments from GFF input files diff --git a/src/uta/exceptions.py b/src/uta/exceptions.py index e391297..4691ed8 100644 --- a/src/uta/exceptions.py +++ b/src/uta/exceptions.py @@ -22,6 +22,10 @@ class EutilsDownloadError(Exception): pass +class ExonStructureMismatchError(UTAError): + pass + + # # Copyright 2014 UTA Contributors (https://bitbucket.org/biocommons/uta) ## diff --git a/src/uta/loading.py b/src/uta/loading.py index 3a93ad3..db2d8f2 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -30,6 +30,7 @@ import uta.formats.txinfo as ufti import uta.parsers.geneinfo import uta.parsers.seqgene +from uta.exceptions import ExonStructureMismatchError usam = uta.models @@ -317,37 +318,41 @@ def load_exonset(session, opts, cf): ) tx_exon_count = len(tx_es.exons_se_i()) aln_exon_count = len(es.exons_se_i.split(";")) - if tx_exon_count != aln_exon_count: - logger.warning( + if tx_exon_count == aln_exon_count: + n, o = _upsert_exon_set_record( + session, es.tx_ac, es.alt_ac, es.strand, es.method, es.exons_se_i + ) + session.commit() + else: + raise ExonStructureMismatchError( "Exon structure mismatch: {tx_exon_count} exons in transcript {es.tx_ac}; {aln_exon_count} in alignment {es.alt_ac}".format( tx_exon_count=tx_exon_count, aln_exon_count=aln_exon_count, es=es, ) ) - skipped = True - continue - - n, o = _upsert_exon_set_record( - session, es.tx_ac, es.alt_ac, es.strand, es.method, es.exons_se_i - ) - session.commit() except IntegrityError as e: logger.exception(e) session.rollback() n_errors += 1 + except NoResultFound as e: + logger.exception(e) + logger.warning("NoResultFound for transcript ExonSet: {es.tx_ac}".format(es=es)) + skipped = True + except ExonStructureMismatchError as e: + logger.exception(e) + skipped = True else: + (no) = (n is not None, o is not None) + if no == (True, False): + n_new += 1 + elif no == (True, True): + n_deprecated += 1 + elif no == (False, True): + n_unchanged += 1 + finally: if skipped: n_skipped += 1 - else: - (no) = (n is not None, o is not None) - if no == (True, False): - n_new += 1 - elif no == (True, True): - n_deprecated += 1 - elif no == (False, True): - n_unchanged += 1 - finally: if i_es % update_period == 0 or i_es + 1 == n_rows: logger.info( "{i_es}/{n_rows} {p:.1f}%; {n_new} new, {n_unchanged} unchanged, {n_deprecated} deprecated, {n_skipped} skipped, {n_errors} n_errors".format( @@ -756,7 +761,7 @@ def _fetch_origin_by_name(name): session.add(usam.TranslationException(**te)) if u_tx.gene_id != ti.gene_id: - raise Exception("{ti.ac}: GeneID changed from {u_tx.gene_id} to {ti.gene_id}".format(u_tx=u_tx, ti=ti)) + logger.warning("{ti.ac}: GeneID changed from {u_tx.gene_id} to {ti.gene_id}".format(u_tx=u_tx, ti=ti)) # state: transcript now exists, either existing or freshly-created From 9e2a927557d1dfb8480c788198ed37937df2539b Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Wed, 15 May 2024 09:01:45 -0600 Subject: [PATCH 49/54] feat(IPVC-2440/IPVC-2442): specify input and output schema names, move one time workflows (#38) --- README.md | 7 ++++--- docker-compose.yml | 16 ++++------------ misc/gene-update/docker-compose-gene-update.yml | 17 +++++++++++++++++ misc/gene-update/upgrade-uta-schema.sh | 10 ++++++---- .../docker-compose-mito-extract.yml | 13 +++++++++++++ sbin/uta-load | 17 ++++++++++++----- 6 files changed, 56 insertions(+), 24 deletions(-) create mode 100644 misc/gene-update/docker-compose-gene-update.yml mode change 100644 => 100755 misc/gene-update/upgrade-uta-schema.sh create mode 100644 misc/mito-transcripts/docker-compose-mito-extract.yml diff --git a/README.md b/README.md index e79452b..8e229cc 100644 --- a/README.md +++ b/README.md @@ -307,6 +307,7 @@ mkdir -p $(pwd)/output/logs Set variables: ``` export UTA_ETL_OLD_SEQREPO_VERSION=2024-02-20 +export UTA_ETL_OLD_UTA_IMAGE_TAG=uta_20210129b export UTA_ETL_OLD_UTA_VERSION=uta_20210129b export UTA_ETL_NCBI_DIR=./ncbi-data export UTA_ETL_SEQREPO_DIR=./seqrepo-data @@ -347,14 +348,14 @@ See 2A for nuclear transcripts and 2B for mitochondrial transcripts. docker compose run ncbi-download docker compose run uta-extract docker compose run seqrepo-load -docker compose run uta-load +UTA_ETL_NEW_UTA_VERSION=uta_20240512 docker compose run uta-load ``` #### 2B. Mitochondrial transcripts ``` -docker compose run mito-extract +docker compose -f docker-compose.yml -f misc/mito-transcripts/docker-compose-mito-extract.yml run mito-extract docker compose run seqrepo-load -docker compose run uta-load +UTA_ETL_NEW_UTA_VERSION=uta_20240512 docker compose run uta-load ``` #### 2C. Manual splign transcripts diff --git a/docker-compose.yml b/docker-compose.yml index aa4081e..2141e7d 100644 --- a/docker-compose.yml +++ b/docker-compose.yml @@ -31,17 +31,17 @@ services: network_mode: host uta: container_name: uta - image: biocommons/uta:${UTA_ETL_OLD_UTA_VERSION} + image: biocommons/uta:${UTA_ETL_OLD_UTA_IMAGE_TAG} environment: - POSTGRES_HOST_AUTH_METHOD=trust healthcheck: - test: psql -h localhost -U anonymous -d uta -c "select * from ${UTA_ETL_OLD_UTA_VERSION}.meta" + test: psql -h localhost -U anonymous -d uta -c "select * from ${UTA_ETL_OLD_UTA_IMAGE_TAG}.meta" interval: 10s - retries: 60 + retries: 80 network_mode: host uta-load: image: uta-update - command: sbin/uta-load ${UTA_ETL_OLD_UTA_VERSION} /ncbi-dir /uta-load/work /uta-load/logs + command: sbin/uta-load ${UTA_ETL_OLD_UTA_VERSION} ${UTA_ETL_NEW_UTA_VERSION} /ncbi-dir /uta-load/work /uta-load/logs depends_on: uta: condition: service_healthy @@ -50,14 +50,6 @@ services: - ${UTA_ETL_WORK_DIR}:/uta-load/work - ${UTA_ETL_LOG_DIR}:/uta-load/logs network_mode: host - mito-extract: - image: uta-update - command: sbin/ncbi_process_mito.py NC_012920.1 --output-dir /mito-extract/work | tee /mito-extract/logs/mito.log - volumes: - - ${UTA_ETL_WORK_DIR}:/mito-extract/work - - ${UTA_ETL_LOG_DIR}:/mito-extract/logs - working_dir: /opt/repos/uta - network_mode: host splign-manual: image: uta-update command: sbin/uta-splign-manual ${UTA_ETL_OLD_SEQREPO_VERSION} ${UTA_ETL_OLD_UTA_VERSION} /uta-splign-manual/input /uta-splign-manual/work /uta-splign-manual/logs diff --git a/misc/gene-update/docker-compose-gene-update.yml b/misc/gene-update/docker-compose-gene-update.yml new file mode 100644 index 0000000..2245f44 --- /dev/null +++ b/misc/gene-update/docker-compose-gene-update.yml @@ -0,0 +1,17 @@ +# docker compose file for the NCBI gene_id update and backfill procedure + +version: '3' + +services: + uta-gene-update: + image: uta-update + command: misc/gene-update/upgrade-uta-schema.sh ${UTA_ETL_NEW_UTA_VERSION} + depends_on: + uta: + condition: service_healthy + volumes: + - ${UTA_ETL_NCBI_DIR}:/ncbi-dir + - ${UTA_ETL_WORK_DIR}:/uta-gene-update/work + - ${UTA_ETL_LOG_DIR}:/uta-gene-update/logs + working_dir: /opt/repos/uta + network_mode: host diff --git a/misc/gene-update/upgrade-uta-schema.sh b/misc/gene-update/upgrade-uta-schema.sh old mode 100644 new mode 100755 index dab0ef0..ba66c0b --- a/misc/gene-update/upgrade-uta-schema.sh +++ b/misc/gene-update/upgrade-uta-schema.sh @@ -17,8 +17,8 @@ set -euxo pipefail source_uta_v="uta_20210129b" working_uta_v="uta" dest_uta_v=$1 -dumps_dir="/workdir/dumps" -mkdir -p $dumps_dir +tmp_dumps_dir="/tmp/dumps" +mkdir -p $tmp_dumps_dir ## setup working uta schema # delete schema if exists @@ -26,10 +26,10 @@ psql -h localhost -U uta_admin -d uta -c "DROP SCHEMA IF EXISTS $working_uta_v C # dump source version pg_dump -U uta_admin -h localhost -d uta -n "$source_uta_v" | \ - gzip -c > $dumps_dir/"$source_uta_v".pgd.gz + gzip -c > $tmp_dumps_dir/"$source_uta_v".pgd.gz # create new schema -gzip -cdq $dumps_dir/"$source_uta_v".pgd.gz | \ +gzip -cdq $tmp_dumps_dir/"$source_uta_v".pgd.gz | \ sbin/pg-dump-schema-rename "$source_uta_v" "$working_uta_v" | \ sbin/pg-dump-schema-rename "uta_1_1" "$working_uta_v" | \ psql -U uta_admin -h localhost -d uta -aeE @@ -53,3 +53,5 @@ alembic -c etc/alembic.ini upgrade head ## Rename schema to destination schema name psql -h localhost -U uta_admin -d uta -c "DROP SCHEMA IF EXISTS $dest_uta_v CASCADE;" psql -h localhost -U uta_admin -d uta -c "ALTER SCHEMA uta RENAME TO $dest_uta_v"; +pg_dump -h localhost -U uta_admin -d uta -n "$dest_uta_v" | \ + gzip -c > "/uta-gene-update/work/$dest_uta_v.pgd.gz" diff --git a/misc/mito-transcripts/docker-compose-mito-extract.yml b/misc/mito-transcripts/docker-compose-mito-extract.yml new file mode 100644 index 0000000..c5a8a0d --- /dev/null +++ b/misc/mito-transcripts/docker-compose-mito-extract.yml @@ -0,0 +1,13 @@ +# docker compose file for the mito transcript extraction for the UTA update procedure + +version: '3' + +services: + mito-extract: + image: uta-update + command: sbin/ncbi_process_mito.py NC_012920.1 --output-dir /mito-extract/work | tee /mito-extract/logs/mito.log + volumes: + - ${UTA_ETL_WORK_DIR}:/mito-extract/work + - ${UTA_ETL_LOG_DIR}:/mito-extract/logs + working_dir: /opt/repos/uta + network_mode: host diff --git a/sbin/uta-load b/sbin/uta-load index 06daec8..ab90405 100755 --- a/sbin/uta-load +++ b/sbin/uta-load @@ -11,13 +11,14 @@ set -euxo pipefail source_uta_v=$1 -ncbi_dir=$2 -working_dir=$3 -log_dir=$4 +dest_uta_v=$2 +ncbi_dir=$3 +working_dir=$4 +log_dir=$5 -if [ -z "$source_uta_v" ] || [ -z "$ncbi_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] +if [ -z "$source_uta_v" ] || [ -z "$dest_uta_v" ] || [ -z "$ncbi_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] then - echo 'Usage: uta-load ' + echo 'Usage: uta-load ' exit 1 fi @@ -66,3 +67,9 @@ uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | ### run diff sbin/uta-diff "$source_uta_v" "$loading_uta_v" + +## Rename schema to destination schema name and export to dump file +psql -h localhost -U uta_admin -d uta -c "DROP SCHEMA IF EXISTS $dest_uta_v CASCADE;" +psql -h localhost -U uta_admin -d uta -c "ALTER SCHEMA uta RENAME TO $dest_uta_v"; +pg_dump -h localhost -U uta_admin -d uta -n "$dest_uta_v" | \ + gzip -c > "$working_dir/$dest_uta_v.pgd.gz" From accee56a7a24b103f3a9f81c8fd7b5ee831e216e Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Tue, 21 May 2024 16:07:50 -0600 Subject: [PATCH 50/54] feat(IPVC-2464): Add a seqrepo-pull compose service (#40) --- README.md | 20 +++--------- docker-compose.yml | 28 ++++++++-------- etc/global.conf | 2 +- .../docker-compose-splign-manual.yml | 16 ++++++++++ .../splign-manual}/uta-splign-manual | 32 +++++++++++-------- sbin/seqrepo-load | 14 ++++---- sbin/seqrepo-pull | 22 +++++++++++++ 7 files changed, 82 insertions(+), 52 deletions(-) create mode 100644 misc/splign-manual/docker-compose-splign-manual.yml rename {sbin => misc/splign-manual}/uta-splign-manual (56%) create mode 100755 sbin/seqrepo-pull diff --git a/README.md b/README.md index 8e229cc..8f9c455 100644 --- a/README.md +++ b/README.md @@ -306,11 +306,10 @@ mkdir -p $(pwd)/output/logs Set variables: ``` -export UTA_ETL_OLD_SEQREPO_VERSION=2024-02-20 export UTA_ETL_OLD_UTA_IMAGE_TAG=uta_20210129b -export UTA_ETL_OLD_UTA_VERSION=uta_20210129b +export UTA_ETL_OLD_UTA_VERSION=UTA_ETL_OLD_UTA_IMAGE_TAG +export UTA_ETL_NEW_UTA_VERSION=uta_20240512 export UTA_ETL_NCBI_DIR=./ncbi-data -export UTA_ETL_SEQREPO_DIR=./seqrepo-data export UTA_ETL_WORK_DIR=./output/artifacts export UTA_ETL_LOG_DIR=./output/logs ``` @@ -322,16 +321,7 @@ docker build --target uta -t uta-update . ### 1. Download SeqRepo data ``` -docker pull biocommons/seqrepo:$UTA_ETL_OLD_SEQREPO_VERSION - -# download seqrepo. can skip if container already exists. -docker run --name seqrepo biocommons/seqrepo:$UTA_ETL_OLD_SEQREPO_VERSION - -# copy seqrepo data into a local directory -docker run -v $UTA_ETL_SEQREPO_DIR:/output-dir --volumes-from seqrepo ubuntu bash -c 'cp -R /usr/local/share/seqrepo/* /output-dir' - -# allow seqrepo to be modified -docker run -it -v $UTA_ETL_SEQREPO_DIR:/output-dir ubuntu bash -c 'chmod -R +w /output-dir' +docker compose run seqrepo-pull ``` Note: pulling data takes ~30 minutes and requires ~13 GB. @@ -348,14 +338,14 @@ See 2A for nuclear transcripts and 2B for mitochondrial transcripts. docker compose run ncbi-download docker compose run uta-extract docker compose run seqrepo-load -UTA_ETL_NEW_UTA_VERSION=uta_20240512 docker compose run uta-load +docker compose run uta-load ``` #### 2B. Mitochondrial transcripts ``` docker compose -f docker-compose.yml -f misc/mito-transcripts/docker-compose-mito-extract.yml run mito-extract docker compose run seqrepo-load -UTA_ETL_NEW_UTA_VERSION=uta_20240512 docker compose run uta-load +docker compose run uta-load ``` #### 2C. Manual splign transcripts diff --git a/docker-compose.yml b/docker-compose.yml index 2141e7d..84b043a 100644 --- a/docker-compose.yml +++ b/docker-compose.yml @@ -3,6 +3,13 @@ version: '3' services: + seqrepo-pull: + user: root + image: uta-update + command: sbin/seqrepo-pull + volumes: + - seqrepo-volume:/biocommons/dl.biocommons.org/seqrepo + network_mode: host ncbi-download: image: uta-update command: sbin/ncbi-download etc/ncbi-files.txt /ncbi-dir @@ -22,9 +29,9 @@ services: network_mode: host seqrepo-load: image: uta-update - command: sbin/seqrepo-load /usr/local/share/seqrepo ${UTA_ETL_OLD_SEQREPO_VERSION} /seqrepo-load/work /seqrepo-load/logs + command: sbin/seqrepo-load /seqrepo-load/work /seqrepo-load/logs volumes: - - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo + - seqrepo-volume:/biocommons/dl.biocommons.org/seqrepo - ${UTA_ETL_WORK_DIR}:/seqrepo-load/work - ${UTA_ETL_LOG_DIR}:/seqrepo-load/logs working_dir: /opt/repos/uta @@ -46,19 +53,10 @@ services: uta: condition: service_healthy volumes: - - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo + - seqrepo-volume:/biocommons/dl.biocommons.org/seqrepo - ${UTA_ETL_WORK_DIR}:/uta-load/work - ${UTA_ETL_LOG_DIR}:/uta-load/logs network_mode: host - splign-manual: - image: uta-update - command: sbin/uta-splign-manual ${UTA_ETL_OLD_SEQREPO_VERSION} ${UTA_ETL_OLD_UTA_VERSION} /uta-splign-manual/input /uta-splign-manual/work /uta-splign-manual/logs - depends_on: - uta: - condition: service_healthy - volumes: - - ${UTA_ETL_SEQREPO_DIR}:/usr/local/share/seqrepo - - ${UTA_SPLIGN_MANUAL_DIR}:/uta-splign-manual/input - - ${UTA_ETL_WORK_DIR}:/uta-splign-manual/work - - ${UTA_ETL_LOG_DIR}:/uta-splign-manual/logs - network_mode: host + +volumes: + seqrepo-volume: diff --git a/etc/global.conf b/etc/global.conf index 1aece93..0d3cfcc 100644 --- a/etc/global.conf +++ b/etc/global.conf @@ -16,7 +16,7 @@ aligner = utaaa fasta_directories = aux/sequences2 aux/sequences -seqrepo = /usr/local/share/seqrepo/2024-02-20 +seqrepo = /biocommons/dl.biocommons.org/seqrepo/master #data/manual #data/bic/sequences.fasta.bgz diff --git a/misc/splign-manual/docker-compose-splign-manual.yml b/misc/splign-manual/docker-compose-splign-manual.yml new file mode 100644 index 0000000..7ef0e87 --- /dev/null +++ b/misc/splign-manual/docker-compose-splign-manual.yml @@ -0,0 +1,16 @@ +# docker compose file for the splign-manual uta update procedure + +version: '3' + +services: + splign-manual: + image: uta-update + command: sbin/uta-splign-manual ${UTA_ETL_OLD_UTA_VERSION} /uta-splign-manual/input /uta-splign-manual/work /uta-splign-manual/logs + depends_on: + uta: + condition: service_healthy + volumes: + - ${UTA_SPLIGN_MANUAL_DIR}:/uta-splign-manual/input + - ${UTA_ETL_WORK_DIR}:/uta-splign-manual/work + - ${UTA_ETL_LOG_DIR}:/uta-splign-manual/logs + network_mode: host diff --git a/sbin/uta-splign-manual b/misc/splign-manual/uta-splign-manual similarity index 56% rename from sbin/uta-splign-manual rename to misc/splign-manual/uta-splign-manual index 2bc3e9b..cb1e9ee 100755 --- a/sbin/uta-splign-manual +++ b/misc/splign-manual/uta-splign-manual @@ -4,15 +4,14 @@ set -euxo pipefail -seqrepo_version=$1 -source_uta_v=$2 -input_dir=$3 -working_dir=$4 -log_dir=$5 +source_uta_v=$1 +input_dir=$2 +working_dir=$3 +log_dir=$4 -if [ -z "$seqrepo_version" ] || [ -z "$source_uta_v" ] || [ -z "$input_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] +if [ -z "$source_uta_v" ] || [ -z "$input_dir" ] || [ -z "$working_dir" ] || [ -z "$log_dir" ] then - echo 'Usage: sbin/uta-splign-manual ' + echo 'Usage: misc/uta-splign-manual ' exit 1 fi @@ -24,22 +23,29 @@ mkdir -p "$log_dir" mkdir -p "$working_dir" # Generate txinfo.gz and exonset.gz files -python sbin/generate-loading-data $input_dir/alignments/*.splign --txdata $input_dir/txdata.yaml --output-dir $working_dir 2>&1 | tee "$log_dir/generate-loading-data.log" +python sbin/generate-loading-data $input_dir/alignments/*.splign --txdata $input_dir/txdata.yaml \ + --output-dir $working_dir 2>&1 | tee "$log_dir/generate-loading-data.log" # Generate fasta files -seqrepo export $(gzip -cdq $working_dir/txinfo.gz | cut -f2 | tail +2) --instance-name "$seqrepo_version" | gzip -c > $working_dir/seqs.fa.gz 2>&1 | tee "$log_dir/seqrepo-export.log" +seqrepo --root-directory "/biocommons/dl.biocommons.org/seqrepo" \ + export $(gzip -cdq $working_dir/txinfo.gz | cut -f2 | tail +2) \ + --instance-name "master" | gzip -c > $working_dir/seqs.fa.gz 2>&1 | tee "$log_dir/seqrepo-export.log" # Generate seqinfo.gz file -sbin/fasta-to-seqinfo -o NCBI $working_dir/seqs.fa.gz | gzip -c > $working_dir/seqinfo.gz 2>&1 | tee "$log_dir/fasta-to-seqinfo.log" +sbin/fasta-to-seqinfo -o NCBI $working_dir/seqs.fa.gz | gzip -c > $working_dir/seqinfo.gz 2>&1 | \ + tee "$log_dir/fasta-to-seqinfo.log" # Load seqinfo -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-seqinfo $working_dir/seqinfo.gz 2>&1 | tee "$log_dir/load-seqinfo.log" +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-seqinfo $working_dir/seqinfo.gz 2>&1 | \ + tee "$log_dir/load-seqinfo.log" # Load txinfo -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo $working_dir/txinfo.gz 2>&1 | tee "$log_dir/load-txinfo.log" +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-txinfo $working_dir/txinfo.gz 2>&1 | \ + tee "$log_dir/load-txinfo.log" # Load exonset -uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset $working_dir/exonset.gz 2>&1 | tee "$log_dir/load-exonset.log" +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf load-exonset $working_dir/exonset.gz 2>&1 | \ + tee "$log_dir/load-exonset.log" # Align exons uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf align-exons 2>&1 | tee "$log_dir/align-exons.log" diff --git a/sbin/seqrepo-load b/sbin/seqrepo-load index 02197ec..95b662f 100755 --- a/sbin/seqrepo-load +++ b/sbin/seqrepo-load @@ -2,14 +2,12 @@ set -euxo pipefail -seqrepo_root=$1 -seqrepo_version=$2 -sequence_dir=$3 -log_dir=$4 +sequence_dir=$1 +log_dir=$2 -if [ -z "$seqrepo_root" ] || [ -z "$seqrepo_version" ] || [ -z "$sequence_dir" ] || [ -z "$log_dir" ] +if [ -z "$sequence_dir" ] || [ -z "$log_dir" ] then - echo 'Usage: sbin/seqrepo-load ' + echo 'Usage: sbin/seqrepo-load ' exit 1 fi @@ -17,7 +15,7 @@ fi mapfile -t FASTA_FILES < <(find "$sequence_dir" -type f -name "*.f[an]a*") # Load SeqRepo with new sequences -seqrepo --root-directory "$seqrepo_root" \ - load -n NCBI --instance-name "$seqrepo_version" \ +seqrepo --root-directory "/biocommons/dl.biocommons.org/seqrepo" \ + load -n NCBI --instance-name "master" \ "${FASTA_FILES[@]}" 2>&1 | \ tee "$log_dir/seqrepo-load.log" diff --git a/sbin/seqrepo-pull b/sbin/seqrepo-pull new file mode 100755 index 0000000..894dffa --- /dev/null +++ b/sbin/seqrepo-pull @@ -0,0 +1,22 @@ +#!/usr/bin/env bash + +set -euxo pipefail + +SEQREPO_DIR="/biocommons/dl.biocommons.org/seqrepo" + +# pull the latest seqrepo version from biocommons +latest_version=$(seqrepo list-remote-instances | tail -n 1 | xargs) +cd "$SEQREPO_DIR" +rsync -rtHP --no-motd dl.biocommons.org::seqrepo/"$latest_version" . + +# setup seqrepo build directory +mkdir -p master/sequences +cd "$latest_version" +cp -av aliases.sqlite3 "$SEQREPO_DIR"/master/ +chmod u+w "$SEQREPO_DIR"/master/aliases.sqlite3 +cd sequences +cp -av db.sqlite3 "$SEQREPO_DIR"/master/sequences/ +chmod u+w "$SEQREPO_DIR"/master/sequences/db.sqlite3 +for d in 2???; do + cp -alv $d "$SEQREPO_DIR"/master/sequences/ +done From 8b6e4d93b2b9ec74b25ed49a675586636246f1a9 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Wed, 22 May 2024 09:13:09 -0600 Subject: [PATCH 51/54] feat(IPVC-2445): add a method to uta.loading to update schema version (#41) --- sbin/uta-load | 3 ++- src/uta/cli.py | 2 ++ src/uta/loading.py | 27 ++++++++++++++++++++++++++ src/uta/models.py | 2 +- tests/test_uta_loading.py | 41 ++++++++++++++++++++++++++++----------- 5 files changed, 62 insertions(+), 13 deletions(-) diff --git a/sbin/uta-load b/sbin/uta-load index ab90405..d419007 100755 --- a/sbin/uta-load +++ b/sbin/uta-load @@ -30,8 +30,9 @@ mkdir -p "$log_dir" etc/scripts/delete-schema.sh "$loading_uta_v" etc/scripts/create-new-schema.sh "$source_uta_v" "$loading_uta_v" -## for now set up Alembic for schema migrations +## apply any outstanding alembic migrations and update schema version if necessary alembic -c etc/alembic.ini upgrade head +uta --conf=etc/global.conf --conf=etc/uta_dev@localhost.conf update-meta-data # generate seqinfo files from exonsets (this step requires seqrepo) sbin/exonset-to-seqinfo -o NCBI "$working_dir/exonsets.gz" | gzip -c > "$working_dir/seqinfo.gz" 2>&1 | \ diff --git a/src/uta/cli.py b/src/uta/cli.py index adc8e8b..bda63b0 100644 --- a/src/uta/cli.py +++ b/src/uta/cli.py @@ -6,6 +6,7 @@ uta (-C CONF ...) [options] shell uta (-C CONF ...) [options] drop-schema uta (-C CONF ...) [options] create-schema + uta (-C CONF ...) [options] update-meta-data uta (-C CONF ...) [options] load-sql FILES ... uta (-C CONF ...) [options] rebuild uta (-C CONF ...) [options] load-origin FILE @@ -68,6 +69,7 @@ def main(): ("align-exons", ul.align_exons), ("analyze", ul.analyze), ("create-schema", ul.create_schema), + ("update-meta-data", ul.update_meta_data), ("drop-schema", ul.drop_schema), ("grant-permissions", ul.grant_permissions), ("load-assoc-ac", ul.load_assoc_ac), diff --git a/src/uta/loading.py b/src/uta/loading.py index db2d8f2..2df1409 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -188,6 +188,33 @@ def create_schema(session, opts, cf): logger.info("created schema") +def update_meta_data(session, opts, cf): + """Update Meta table with schema version""" + session.execute(text("set role {admin_role};".format( + admin_role=cf.get("uta", "admin_role")))) + session.execute(text("set search_path = " + usam.schema_name)) + + # check if schema version is up-to-date + md_schema_version = session.query(usam.Meta).filter_by(key="schema_version").one() + if md_schema_version.value != usam.schema_version: + logger.info(f"updating schema version from {md_schema_version.value} to {usam.schema_version}") + md_schema_version.value = usam.schema_version + session.commit() + else: + logger.info(f"schema version {md_schema_version.value} is already up-to-date") + + # set updated on + md_updated_on = session.query(usam.Meta).filter_by(key="updated on").one_or_none() + if md_updated_on is None: + session.add(usam.Meta(key="updated on", value=datetime.datetime.now().isoformat())) + session.commit() + logger.info("added updated on") + else: + md_updated_on.value = datetime.datetime.now().isoformat() + session.commit() + logger.info("updated updated on") + + def drop_schema(session, opts, cf): if session.bind.name == "postgresql" and usam.use_schema: session.execute( diff --git a/src/uta/models.py b/src/uta/models.py index 3f3076a..6dea5ca 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -16,7 +16,7 @@ # schema name support # also see etc/uta.conf -schema_version = "1.1" +schema_version = "1.2" use_schema = True if use_schema: schema_name = "uta" diff --git a/tests/test_uta_loading.py b/tests/test_uta_loading.py index 5036506..49188c5 100644 --- a/tests/test_uta_loading.py +++ b/tests/test_uta_loading.py @@ -14,26 +14,45 @@ class TestUtaLoading(unittest.TestCase): def setUp(self): + # setup test database self.db = testing.postgresql.Postgresql() self.session = uta.connect(self.db.url()) - schema = usam.schema_name - self.session.execute(sa.text(f'drop schema if exists {schema} cascade')) - self.session.execute(sa.text(f'create schema {schema}')) - self.session.execute(sa.text('create role uta_admin')) - self.session.execute(sa.text(f'grant all privileges on schema {schema} to uta_admin')) - self.session.commit() - # create all uta tables - usam.Base.metadata.create_all(self.session.bind.engine) - self.session.execute(sa.text(f'grant all privileges on all tables in schema {schema} to uta_admin')) - self.session.execute(sa.text(f'grant all privileges on all sequences in schema {schema} to uta_admin')) - self.session.commit() + admin_role = 'uta_admin' + self.session.execute(sa.text(f'create user {admin_role}')) + self.session.execute(sa.text(f'grant all privileges on database test to {admin_role}')) + + self.cf = configparser.ConfigParser() + self.cf.add_section('uta') + self.cf.set('uta', 'admin_role', 'uta_admin') + + ul.create_schema(self.session, {}, self.cf) + ul.grant_permissions(self.session, {}, self.cf) def tearDown(self): self.session.close() self.db.stop(_signal=signal.SIGKILL) self.db.cleanup() + def test_meta_data(self): + """ + Metadata should exist, then updated when update_meta_data is called. + """ + # the schema_version should match existing values in UTA models + expected_schema_version = usam.schema_version + md_schema_version = self.session.query(usam.Meta).filter(usam.Meta.key == 'schema_version').one() + self.assertEqual(md_schema_version.value, expected_schema_version) + + new_schema_version = '9.9' + with patch('uta.models.schema_version', new_schema_version): + ul.update_meta_data(self.session, {}, self.cf) + + md_schema_version = self.session.query(usam.Meta).filter(usam.Meta.key == 'schema_version').one() + self.assertEqual(md_schema_version.value, new_schema_version) + + md_updated_at = self.session.query(usam.Meta).filter(usam.Meta.key == 'updated on').one_or_none() + self.assertIsNotNone(md_updated_at) + def test_load_assoc_ac(self): """ Loading file tests/data/assocacs.gz should create associated_accessions records in the database. From e8c811c02211d733b647b3c29d12cdb572e81568 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Thu, 23 May 2024 12:47:06 -0600 Subject: [PATCH 52/54] feat(IPVC-2471): add codon_table to txinfo file (#42) --- sbin/ncbi_process_mito.py | 1 + ...7b584f699_add_codon_table_to_transcript.py | 5 ++- src/uta/formats/txinfo.py | 2 +- src/uta/loading.py | 3 ++ src/uta/parsers/seqrecord.py | 9 +++++ tests/data/txinfo.gz | Bin 164 -> 215 bytes tests/test_uta_loading.py | 31 +++++++++++++++++- tests/test_uta_parsers_seqrecord.py | 16 ++++----- 8 files changed, 54 insertions(+), 13 deletions(-) diff --git a/sbin/ncbi_process_mito.py b/sbin/ncbi_process_mito.py index 51190f7..b414f2e 100755 --- a/sbin/ncbi_process_mito.py +++ b/sbin/ncbi_process_mito.py @@ -341,6 +341,7 @@ def main(ncbi_accession: str, output_dir: str) -> None: mg.gene_symbol, mg.cds_se_i(), mg.exons_se_i(), + mg.transl_table, TxInfo.serialize_transl_except(mg.transl_except), ) ) diff --git a/src/alembic/versions/a697b584f699_add_codon_table_to_transcript.py b/src/alembic/versions/a697b584f699_add_codon_table_to_transcript.py index cb6eec1..d440495 100644 --- a/src/alembic/versions/a697b584f699_add_codon_table_to_transcript.py +++ b/src/alembic/versions/a697b584f699_add_codon_table_to_transcript.py @@ -20,7 +20,10 @@ def upgrade() -> None: # ### commands auto generated by Alembic - please adjust! ### - op.add_column('transcript', sa.Column('codon_table', sa.Integer(), server_default='1', nullable=False), schema='uta') + op.add_column('transcript', sa.Column('codon_table', sa.Text(), nullable=True), schema='uta') + # ### end Alembic commands ### + # ### population of codon_table column with data ### + op.execute("UPDATE transcript SET codon_table = '1' WHERE cds_start_i NOTNULL;") # ### end Alembic commands ### diff --git a/src/uta/formats/txinfo.py b/src/uta/formats/txinfo.py index 9e812bf..42660a4 100644 --- a/src/uta/formats/txinfo.py +++ b/src/uta/formats/txinfo.py @@ -8,7 +8,7 @@ class TxInfo( recordtype.recordtype( 'TxInfo', - ['origin', 'ac', 'gene_id', 'gene_symbol', 'cds_se_i', 'exons_se_i', 'transl_except'], + ['origin', 'ac', 'gene_id', 'gene_symbol', 'cds_se_i', 'exons_se_i', 'codon_table', 'transl_except'], )): @staticmethod diff --git a/src/uta/loading.py b/src/uta/loading.py index 2df1409..e5fa03d 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -707,8 +707,10 @@ def _fetch_origin_by_name(name): if ti.cds_se_i: cds_start_i, cds_end_i = map(int, ti.cds_se_i.split(",")) + codon_table = ti.codon_table else: cds_start_i = cds_end_i = None + codon_table = None cds_md5 = None # 1. Fetch or make the Transcript record @@ -776,6 +778,7 @@ def _fetch_origin_by_name(name): cds_start_i=cds_start_i, cds_end_i=cds_end_i, cds_md5=cds_md5, + codon_table=codon_table, ) session.add(u_tx) diff --git a/src/uta/parsers/seqrecord.py b/src/uta/parsers/seqrecord.py index dd030e2..9168b42 100644 --- a/src/uta/parsers/seqrecord.py +++ b/src/uta/parsers/seqrecord.py @@ -168,6 +168,15 @@ def exons_se_i(self): se_i = [(f.location.start.real, f.location.end.real) for f in exons] return se_i + @property + def codon_table(self) -> Optional[str]: + if self.cds_feature is None: + return None + else: + # default codon table is the standard table, aka "1" + # https://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi + return "1" + @property def transl_except(self) -> Optional[List[str]]: if self.cds_feature is None: diff --git a/tests/data/txinfo.gz b/tests/data/txinfo.gz index a2f4776388f3de042dd9f763c3b5281ff13f4a93..8b3315327e6e04405af62cf6b546ebbcf342079b 100644 GIT binary patch literal 215 zcmV;|04V<-iwFoL!A@oX19W(4Zf0))Esa5|!Y~ko&+9Ky$Sa#o8XJ4F^dP)eQ12n8 zK?*ey(}VtdBlhskEX(dpe2%vCg+`=$6w>;{R#`%G3uCJa7Xa}xo^hDMZ3egb Date: Wed, 10 Jul 2024 14:00:20 -0600 Subject: [PATCH 53/54] feat(IPVC-2478): updates/fixes to UTA files found during testing (#43) --- etc/uta_dev@uic.com | 4 +- misc/export_mappings.py | 184 ++++++++++++++++++ misc/generate_alignment_metrics.py | 7 +- .../ncbi_extract_gbff.py | 1 + pyproject.toml | 1 + sbin/ncbi-parse-gbff | 1 + sbin/update-ncbi | 2 +- .../versions/edadb97f6502_initial_state.py | 4 + ...et_gene_id_and_primary_and_foreign_keys.py | 1 + src/uta/loading.py | 3 +- 10 files changed, 199 insertions(+), 9 deletions(-) create mode 100644 misc/export_mappings.py diff --git a/etc/uta_dev@uic.com b/etc/uta_dev@uic.com index e61a48b..cbf2e77 100644 --- a/etc/uta_dev@uic.com +++ b/etc/uta_dev@uic.com @@ -1,4 +1,4 @@ [uta] -hostport = uta.invitae.com +hostport = uta-int-02.cj7o8ef9mt4v.us-east-1.rds.amazonaws.com user = uta_admin -database = uta_dev +database = uta diff --git a/misc/export_mappings.py b/misc/export_mappings.py new file mode 100644 index 0000000..ded3ddb --- /dev/null +++ b/misc/export_mappings.py @@ -0,0 +1,184 @@ +import argparse +import logging +import re +from bioutils.assemblies import make_name_ac_map +from contextlib import ExitStack +from dataclasses import dataclass, field + +import psycopg2 +import six + +import uta + +logging.basicConfig(format='%(asctime)s %(levelname)-8s %(message)s', level=logging.INFO, datefmt='%Y-%m-%d %H:%M:%S') +logger = logging.getLogger("export_mappings") + + +UTA_SCHEMA_VERSION_SQL = """ +select value as schema_version +from meta +where key='schema_version'; +""" + +ASSOCIATED_ACCESSIONS_SQL = """ +select aa.tx_ac, aa.pro_ac +from associated_accessions as aa +where aa.tx_ac='{}'; +""" + +# get_tx_mapping_options +TX_MAPPING_OPTIONS_SQL = """ +select distinct tx_ac,alt_ac,alt_aln_method +from tx_exon_aln_v where tx_ac='{}' and exon_aln_id is not NULL +order by alt_ac,alt_aln_method; +""" + +# get_tx_info +TX_V1_INFO_SQL = """ +select hgnc, cds_start_i, cds_end_i, tx_ac, alt_ac, alt_aln_method +from transcript T +join exon_set ES on T.ac=ES.tx_ac +where tx_ac='{}' and alt_ac='{}' and alt_aln_method='{}'; +""" + +TX_V2_INFO_SQL = """ +select G.hgnc, T.cds_start_i, T.cds_end_i, ES.tx_ac, ES.alt_ac, ES.alt_aln_method +from gene G +join transcript T on G.gene_id=T.gene_id +join exon_set ES on T.ac=ES.tx_ac +where tx_ac='{}' and alt_ac='{}' and alt_aln_method='{}'; +""" + +EXON_SET_SQL = """ +select * +from tx_exon_aln_v +where tx_ac='{}' and alt_ac='{}' and alt_aln_method='{}' +order by alt_start_i; +""" + +TX_INDENTITY_SQL = """ +select distinct(tx_ac), alt_ac, alt_aln_method, cds_start_i, cds_end_i, lengths, hgnc +from tx_def_summary_v +where tx_ac='{}'; +""" + + +def parse_args(): + parser = argparse.ArgumentParser( + description="Export transcript alignments for a given genome build from UTA database." + ) + parser.add_argument("transcripts_file", type=str) + parser.add_argument("--genome-build", type=str, default="GRCh37.p13") + parser.add_argument("--db-url", default="postgresql://uta_admin@localhost/uta") + parser.add_argument("--schema-name", default="uta_20210129") + return parser.parse_args() + + +def _get_cursor(con, schema_name): + cur = con.cursor(cursor_factory=psycopg2.extras.NamedTupleCursor) + cur.execute(f"set search_path = {schema_name}") + return cur + + +def _get_rows(cur, sql): + cur.execute(sql) + return cur.fetchall() + + +def _get_chr_ac_map(genome_build): + filtered_chr_ac_map = {} + + def_name_ac_map = make_name_ac_map(assy_name=genome_build, primary_only=True) + for chr_name in list(map(str, range(1, 23))) + ["X", "Y"]: + filtered_chr_ac_map[chr_name] = def_name_ac_map.get(chr_name) + + return filtered_chr_ac_map + + +def main(transcripts_file, genome_build, db_url, schema_name): + logger.info(f"connecting to {db_url}") + session = uta.connect(db_url) + + con = session.bind.pool.connect() + cur = _get_cursor(con, schema_name) + + chr_to_acc_dict = _get_chr_ac_map(genome_build=genome_build) + + schema_version = _get_rows(cur, UTA_SCHEMA_VERSION_SQL)[0].schema_version + if schema_version == "1.1": + TX_INFO_SQL = TX_V1_INFO_SQL + else: + TX_INFO_SQL = TX_V2_INFO_SQL + + # read in transcripts + transcripts = [] + with open(transcripts_file, "r") as f: + for line in f: + if line.startswith("accession"): + continue + accession, chrom = line.rstrip("\r\n").split("\t") + transcripts.append((accession, chrom)) + + # setup context managers for file writers + with ExitStack() as stack: + # associated acccessions + assocacs_fh = stack.enter_context( + open(f"{schema_name}_associated_accessions.tsv", "w") + ) + assocacs_fh.write("tx_ac\tpro_ac\n") + + # transcript info + txinfo_fh = stack.enter_context(open(f"{schema_name}_transcript_info.tsv", "w")) + txinfo_fh.write("hgnc\ttx_ac\tcds_start_i\tcds_end_i\talt_ac\talt_aln_method\n") + + # exon sets + exons_fh = stack.enter_context(open(f"{schema_name}_exon_sets.tsv", "w")) + exons_fh.write("hgnc\ttx_ac\talt_ac\talt_aln_method\talt_strand\tords\ttx_ac_se_i\talt_ac_se_i\tcigars\n") + + # transcript identity + tx_identity_fh = stack.enter_context( + open(f"{schema_name}_transcript_identity.tsv", "w") + ) + + logger.info("querying database for transcript mappings...") + for i, (tx_ac, chrom) in enumerate(transcripts): + assocacs_rows = _get_rows(cur, ASSOCIATED_ACCESSIONS_SQL.format(tx_ac)) + for row in assocacs_rows: + assocacs_fh.write(f"{row.tx_ac}\t{row.pro_ac}\n") + + alt_ac = chr_to_acc_dict.get(chrom) + for alt_aln_method in ("splign", "splign-manual"): + txinfo_rows = _get_rows( + cur, TX_INFO_SQL.format(tx_ac, alt_ac, alt_aln_method) + ) + if txinfo_rows: + for row in txinfo_rows: + txinfo_fh.write( + f"{row.hgnc}\t{row.tx_ac}\t{row.cds_start_i}\t{row.cds_end_i}\t{row.alt_ac}\t{row.alt_aln_method}\n" + ) + exons_rows = _get_rows( + cur, EXON_SET_SQL.format(tx_ac, alt_ac, alt_aln_method) + ) + if exons_rows: + hgnc = exons_rows[0].hgnc + tx_ac = exons_rows[0].tx_ac + alt_ac = exons_rows[0].alt_ac + alt_aln_method = exons_rows[0].alt_aln_method + alt_strand = exons_rows[0].alt_strand + ords, tx_ac_se_i, alt_ac_se_i, cigars = [], [], [], [] + for row in sorted(exons_rows, key=lambda x: x.ord): + ords.append(str(row.ord)) + tx_ac_se_i.append(f"{row.tx_start_i},{row.tx_end_i}") + alt_ac_se_i.append(f"{row.alt_start_i},{row.alt_end_i}") + cigars.append(row.cigar) + exons_fh.write( + f"{hgnc}\t{tx_ac}\t{alt_ac}\t{alt_aln_method}\t{alt_strand}\t{';'.join(ords)}\t{';'.join(tx_ac_se_i)}\t{';'.join(alt_ac_se_i)}\t{';'.join(cigars)}\n" + ) + + if i % 2500 == 0 and i > 0: + logger.info(f"processed {i} transcripts") + + +if __name__ == '__main__': + arguments = parse_args() + main(arguments.transcripts_file, arguments.genome_build, arguments.db_url, arguments.schema_name) diff --git a/misc/generate_alignment_metrics.py b/misc/generate_alignment_metrics.py index 0e51c8f..d0e0e7f 100644 --- a/misc/generate_alignment_metrics.py +++ b/misc/generate_alignment_metrics.py @@ -179,7 +179,7 @@ def coverage(self): TX_EXON_SET_SUMMARY_ALL_BUILD37_SQL = """ select * -from uta_20210129b.tx_exon_set_summary_mv as mv +from tx_exon_set_summary_mv as mv where mv.alt_aln_method in ('splign', 'splign-manual') and mv.tx_ac ~ 'N[MR]_*' and mv.tx_ac !~ '/' and mv.alt_ac in ('NC_000001.10', 'NC_000002.11', 'NC_000003.11', 'NC_000004.11', 'NC_000005.9', 'NC_000006.11', 'NC_000007.13', 'NC_000008.10', 'NC_000009.11', 'NC_000010.10', 'NC_000011.9', 'NC_000012.11', @@ -190,14 +190,14 @@ def coverage(self): TX_EXON_SET_SUMMARY_SQL = """ select mv.ends_i[mv.n_exons] as tx_length, * -from uta_20210129b.tx_exon_set_summary_mv as mv +from tx_exon_set_summary_mv as mv where mv.tx_ac='{tx_ac}' and mv.alt_ac='{alt_ac}' and mv.alt_aln_method='{alt_aln_method}'; """ TX_EXON_ALN_SQL = """ select v.hgnc, v.tx_ac, v.alt_ac, v.alt_aln_method, v.alt_strand, v.ord, v.tx_start_i, v.tx_end_i, v.alt_start_i, v.alt_end_i, v.cigar -from uta_20210129b.tx_exon_aln_v as v +from tx_exon_aln_v as v where tx_ac='{tx_ac}' and alt_ac='{alt_ac}' and alt_aln_method='{alt_aln_method}' order by ord; """ @@ -213,7 +213,6 @@ def parse_args(): def _get_cursor(con, schema_name): cur = con.cursor(cursor_factory=psycopg2.extras.NamedTupleCursor) - cur.execute("set role uta_admin;") cur.execute(f"set search_path = {schema_name}") return cur diff --git a/misc/refseq-historical-backfill/ncbi_extract_gbff.py b/misc/refseq-historical-backfill/ncbi_extract_gbff.py index 4b877e1..cab3277 100755 --- a/misc/refseq-historical-backfill/ncbi_extract_gbff.py +++ b/misc/refseq-historical-backfill/ncbi_extract_gbff.py @@ -132,6 +132,7 @@ def main(gbff_files: Iterable, origin: str, prefix: str, output_dir: str) -> Non gene_symbol=srf.gene_symbol, cds_se_i=TxInfo.serialize_cds_se_i(srf.cds_se_i), exons_se_i=TxInfo.serialize_exons_se_i(srf.exons_se_i), + codon_table=srf.codon_table, transl_except=TxInfo.serialize_transl_except( srf.transl_except ), diff --git a/pyproject.toml b/pyproject.toml index 652c22d..af4e7e9 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -55,6 +55,7 @@ dependencies = [ "psycopg2-binary", "pytz", "recordtype", + "retry", "sqlalchemy", "uta-align>=0.3", ] diff --git a/sbin/ncbi-parse-gbff b/sbin/ncbi-parse-gbff index 9850d4c..110f01a 100755 --- a/sbin/ncbi-parse-gbff +++ b/sbin/ncbi-parse-gbff @@ -118,6 +118,7 @@ if __name__ == "__main__": gene_symbol=srf.gene_symbol, cds_se_i=TxInfo.serialize_cds_se_i(srf.cds_se_i), exons_se_i=TxInfo.serialize_exons_se_i(srf.exons_se_i), + codon_table=srf.codon_table, transl_except=TxInfo.serialize_transl_except(srf.transl_except), ) tiw.write(ti) diff --git a/sbin/update-ncbi b/sbin/update-ncbi index 33211c7..41271d3 100755 --- a/sbin/update-ncbi +++ b/sbin/update-ncbi @@ -88,7 +88,7 @@ if __name__ == "__main__": logging.basicConfig(level=logging.DEBUG) logger = logging.getLogger() - url = "postgresql://uta_admin@localhost/uta_dev" + url = "postgresql://uta_admin@localhost/uta" uta, ncbi = sys.argv[1:3] conn = psycopg2.connect(url) diff --git a/src/alembic/versions/edadb97f6502_initial_state.py b/src/alembic/versions/edadb97f6502_initial_state.py index 86ea9cd..ba46093 100644 --- a/src/alembic/versions/edadb97f6502_initial_state.py +++ b/src/alembic/versions/edadb97f6502_initial_state.py @@ -299,6 +299,9 @@ def upgrade() -> None: op.execute(""" CREATE MATERIALIZED VIEW tx_def_summary_mv AS SELECT * FROM tx_def_summary_dv WITH NO DATA; """) + op.execute(""" + CREATE VIEW tx_def_summary_v AS SELECT * FROM tx_def_summary_mv; + """) op.execute(""" COMMENT ON MATERIALIZED VIEW tx_def_summary_mv IS 'transcript definitions, with exon structures and fingerprints'; """) @@ -341,6 +344,7 @@ def downgrade() -> None: op.execute("DROP INDEX tx_def_summary_mv_alt_aln_method CASCADE") op.execute("DROP INDEX tx_def_summary_mv_alt_ac CASCADE") op.execute("DROP INDEX tx_def_summary_mv_tx_ac CASCADE") + op.execute("DROP VIEW tx_def_summary_v CASCADE;") op.execute("DROP MATERIALIZED VIEW tx_def_summary_mv CASCADE;") op.execute("DROP VIEW tx_def_summary_dv CASCADE;") op.execute("DROP MATERIALIZED VIEW tx_exon_set_summary_mv CASCADE;") diff --git a/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py b/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py index 62cabe1..446fae1 100644 --- a/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py +++ b/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py @@ -154,6 +154,7 @@ def upgrade() -> None: CREATE VIEW tx_similarity_v AS SELECT DISTINCT D1.tx_ac as tx_ac1, D2.tx_ac as tx_ac2, + D1.hgnc = D2.hgnc as hgnc_eq, D1.symbol = D2.symbol as symbol_eq, D1.cds_md5=D2.cds_md5 as cds_eq, D1.es_fingerprint=D2.es_fingerprint as es_fp_eq, diff --git a/src/uta/loading.py b/src/uta/loading.py index e5fa03d..3301c40 100644 --- a/src/uta/loading.py +++ b/src/uta/loading.py @@ -873,8 +873,7 @@ def refresh_matviews(session, opts, cf): "refresh materialized view exon_set_exons_fp_mv", "refresh materialized view tx_exon_set_summary_mv", "refresh materialized view tx_def_summary_mv", - # "refresh materialized view tx_aln_cigar_mv", - # "refresh materialized view tx_aln_summary_mv", + "refresh materialized view tx_exon_aln_mv", ] for cmd in cmds: From 684c8040a59c3e269ec5fb9a3b1c23cfe7480518 Mon Sep 17 00:00:00 2001 From: Shane Giles <62901608+bsgiles73@users.noreply.github.com> Date: Mon, 26 Aug 2024 17:15:19 -0600 Subject: [PATCH 54/54] feat(/IPVC-2695): Updates needed to retain HGNC on transcript (#45) --- .../77076df4224c_add_tx_hgnc_index.py | 30 +++++++++++++++++++ ...et_gene_id_and_primary_and_foreign_keys.py | 8 ----- src/uta/models.py | 3 +- tests/test_uta_loading.py | 4 +-- 4 files changed, 34 insertions(+), 11 deletions(-) create mode 100644 src/alembic/versions/77076df4224c_add_tx_hgnc_index.py diff --git a/src/alembic/versions/77076df4224c_add_tx_hgnc_index.py b/src/alembic/versions/77076df4224c_add_tx_hgnc_index.py new file mode 100644 index 0000000..0a684a2 --- /dev/null +++ b/src/alembic/versions/77076df4224c_add_tx_hgnc_index.py @@ -0,0 +1,30 @@ +"""hgnc test + +Revision ID: 77076df4224c +Revises: 19561fe444c8 +Create Date: 2024-08-26 17:08:13.160259 + +""" +from typing import Sequence, Union + +from alembic import op +import sqlalchemy as sa + + +# revision identifiers, used by Alembic. +revision: str = '77076df4224c' +down_revision: Union[str, None] = '19561fe444c8' +branch_labels: Union[str, Sequence[str], None] = None +depends_on: Union[str, Sequence[str], None] = None + + +def upgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.create_index(op.f('ix_uta_transcript_hgnc'), 'transcript', ['hgnc'], unique=False, schema='uta') + # ### end Alembic commands ### + + +def downgrade() -> None: + # ### commands auto generated by Alembic - please adjust! ### + op.drop_index(op.f('ix_uta_transcript_hgnc'), table_name='transcript', schema='uta') + # ### end Alembic commands ### diff --git a/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py b/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py index 446fae1..1548b95 100644 --- a/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py +++ b/src/alembic/versions/f85dd97bd9f5_set_gene_id_and_primary_and_foreign_keys.py @@ -171,16 +171,8 @@ def upgrade() -> None: """) # ### end of updates to existing views ### - # ### drop hgnc from transcript ### - op.drop_column('transcript', 'hgnc', schema='uta') - # ### end Alembic commands ### - def downgrade() -> None: - # ### updates to views to add hgnc to transcript ### - op.add_column('transcript', sa.Column('hgnc', sa.Text(), nullable=True), schema='uta') - # ### end of updates to transcript ### - # ### commands to downgrade views before adding hgnc to transcript ### op.execute("DROP VIEW IF EXISTS tx_similarity_v CASCADE;") op.execute("DROP MATERIALIZED VIEW IF EXISTS tx_def_summary_mv CASCADE;") diff --git a/src/uta/models.py b/src/uta/models.py index 6dea5ca..a8ec1dd 100644 --- a/src/uta/models.py +++ b/src/uta/models.py @@ -129,12 +129,13 @@ class Transcript(Base): origin_id = sa.Column( sa.Integer, sa.ForeignKey("origin.origin_id", onupdate="CASCADE", ondelete="CASCADE"), nullable=False, index=True) gene_id = sa.Column(sa.Text, sa.ForeignKey("gene.gene_id"), nullable=False, index=True) + hgnc = sa.Column(sa.Text, nullable=True, index=True) cds_start_i = sa.Column(sa.Integer) cds_end_i = sa.Column(sa.Integer) cds_md5 = sa.Column(sa.Text, index=True) added = sa.Column( sa.DateTime, default=datetime.datetime.now(), nullable=False) - codon_table = sa.Column(sa.Integer, nullable=False, server_default='1') # 1 = standard, 2 = mitochondrial + codon_table = sa.Column(sa.Text, nullable=True, server_default='1') # 1 = standard, 2 = mitochondrial # relationships: origin = sao.relationship("Origin", backref="transcripts") diff --git a/tests/test_uta_loading.py b/tests/test_uta_loading.py index 902ccfb..210d2ab 100644 --- a/tests/test_uta_loading.py +++ b/tests/test_uta_loading.py @@ -214,7 +214,7 @@ def test_load_txinfo(self): 'gene_id': '140606', 'cds_start_i': 63, 'cds_end_i': 501, - 'codon_table': 1, + 'codon_table': '1', }, ) @@ -232,7 +232,7 @@ def test_load_txinfo(self): 'gene_id': '4514', 'cds_start_i': 0, 'cds_end_i': 784, - 'codon_table': 2, + 'codon_table': '2', }, )