diff --git a/pkgs/applications/graphics/hydrus/default.nix b/pkgs/applications/graphics/hydrus/default.nix
index 7da1858cb542a..8f2891639751a 100644
--- a/pkgs/applications/graphics/hydrus/default.nix
+++ b/pkgs/applications/graphics/hydrus/default.nix
@@ -6,7 +6,6 @@
 , enableSwftools ? false
 , swftools
 , python3Packages
-, pythonOlder
 , qtbase
 , qtcharts
 , makeDesktopItem
@@ -82,9 +81,6 @@ python3Packages.buildPythonPackage rec {
     twisted
   ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = with python3Packages; [
     nose
     mock
diff --git a/pkgs/development/python-modules/actdiag/default.nix b/pkgs/development/python-modules/actdiag/default.nix
index cf84a064b54f0..effba4c188bb6 100644
--- a/pkgs/development/python-modules/actdiag/default.nix
+++ b/pkgs/development/python-modules/actdiag/default.nix
@@ -27,9 +27,6 @@ buildPythonPackage rec {
 
   propagatedBuildInputs = [ blockdiag ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     nose
     pytestCheckHook
diff --git a/pkgs/development/python-modules/biopandas/default.nix b/pkgs/development/python-modules/biopandas/default.nix
index be59f3638d913..1102457cb94d4 100644
--- a/pkgs/development/python-modules/biopandas/default.nix
+++ b/pkgs/development/python-modules/biopandas/default.nix
@@ -35,10 +35,6 @@ buildPythonPackage rec {
     looseversion
   ];
 
-  # tests rely on nose
-  # resolved in 0.5.1: https://github.com/BioPandas/biopandas/commit/67aa2f237c70c826cd9ab59d6ae114582da2112f
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     nose
     pytestCheckHook
diff --git a/pkgs/development/python-modules/blockdiag/default.nix b/pkgs/development/python-modules/blockdiag/default.nix
index 8b43fd2c90656..8b0742ed77f5b 100644
--- a/pkgs/development/python-modules/blockdiag/default.nix
+++ b/pkgs/development/python-modules/blockdiag/default.nix
@@ -48,9 +48,6 @@ buildPythonPackage rec {
     webcolors
   ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     ephem
     nose
diff --git a/pkgs/development/python-modules/hkdf/default.nix b/pkgs/development/python-modules/hkdf/default.nix
index 176322d932212..f04e486fd73ca 100644
--- a/pkgs/development/python-modules/hkdf/default.nix
+++ b/pkgs/development/python-modules/hkdf/default.nix
@@ -2,7 +2,6 @@
   lib,
   buildPythonPackage,
   fetchFromGitHub,
-  pythonOlder,
   nose,
   setuptools,
 }:
@@ -23,9 +22,6 @@ buildPythonPackage {
 
   pythonImportsCheck = [ "hkdf" ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [ nose ];
 
   checkPhase = ''
diff --git a/pkgs/development/python-modules/lockfile/default.nix b/pkgs/development/python-modules/lockfile/default.nix
index 0a0b906e91229..5423a1abb300f 100644
--- a/pkgs/development/python-modules/lockfile/default.nix
+++ b/pkgs/development/python-modules/lockfile/default.nix
@@ -5,7 +5,6 @@
   setuptools,
   pbr,
   nose,
-  pythonOlder,
 }:
 
 buildPythonPackage rec {
@@ -23,9 +22,6 @@ buildPythonPackage rec {
     setuptools
   ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [ nose ];
 
   checkPhase = ''
diff --git a/pkgs/development/python-modules/nose/default.nix b/pkgs/development/python-modules/nose/default.nix
index f864cfb9125f3..44b98b0620e78 100644
--- a/pkgs/development/python-modules/nose/default.nix
+++ b/pkgs/development/python-modules/nose/default.nix
@@ -5,23 +5,35 @@
   isPy3k,
   isPyPy,
   python,
-  pythonAtLeast,
+  python312,
   coverage,
+  setuptools,
+  fetchpatch2,
 }:
 
 buildPythonPackage rec {
   version = "1.3.7";
-  format = "setuptools";
   pname = "nose";
-
-  # unmaintained, relies on the imp module
-  disabled = pythonAtLeast "3.12";
+  pyproject = true;
 
   src = fetchPypi {
     inherit pname version;
     sha256 = "f1bffef9cbc82628f6e7d7b40d7e255aefaa1adb6a1b1d26c69a8b79e6208a98";
   };
 
+  build-system = [ setuptools ];
+
+  patches = [
+    (fetchpatch2 {
+      url = "https://gitlab.alpinelinux.org/alpine/aports/-/raw/71a773b0618ce60d276a3df74ed69e6bd6d13237/community/py3-nose/python-nose-py311.patch";
+      hash = "sha256-CwY1pxzFFD0AYgmbERQyb6dD3bSInTqteUM85nfYkq8=";
+    })
+    (fetchpatch2 {
+      url = "https://gitlab.alpinelinux.org/alpine/aports/-/raw/71a773b0618ce60d276a3df74ed69e6bd6d13237/community/py3-nose/python-nose-py312.patch";
+      hash = "sha256-/mJHbal8XXOWvLtebrYmoK23rCNp+VWIFYDPUckEEGg=";
+    })
+  ];
+
   # 2to3 was removed in setuptools 58
   postPatch = ''
     substituteInPlace setup.py \
@@ -31,8 +43,9 @@ buildPythonPackage rec {
       --replace "from setuptools.command.build_py import Mixin2to3" "from distutils.util import Mixin2to3"
   '';
 
-  preBuild = lib.optionalString (isPy3k) ''
-    ${python.pythonOnBuildForHost}/bin/2to3 -wn nose functional_tests unit_tests
+  # Python 3.12 is the last version to ship 2to3.
+  preBuild = lib.optionalString isPy3k ''
+    ${python312.pythonOnBuildForHost}/bin/2to3 -wn nose functional_tests unit_tests
   '';
 
   propagatedBuildInputs = [ coverage ];
diff --git a/pkgs/development/python-modules/nwdiag/default.nix b/pkgs/development/python-modules/nwdiag/default.nix
index dc2fd74fbef56..ac121549596ee 100644
--- a/pkgs/development/python-modules/nwdiag/default.nix
+++ b/pkgs/development/python-modules/nwdiag/default.nix
@@ -27,9 +27,6 @@ buildPythonPackage rec {
 
   dependencies = [ blockdiag ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     nose
     pytestCheckHook
diff --git a/pkgs/development/python-modules/pprintpp/default.nix b/pkgs/development/python-modules/pprintpp/default.nix
index d7da3d5511e8b..904e067b6c0a8 100644
--- a/pkgs/development/python-modules/pprintpp/default.nix
+++ b/pkgs/development/python-modules/pprintpp/default.nix
@@ -38,9 +38,6 @@ buildPythonPackage rec {
 
   build-system = [ setuptools ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     nose
     parameterized
diff --git a/pkgs/development/python-modules/pydy/default.nix b/pkgs/development/python-modules/pydy/default.nix
index 1c858f5d02914..e9b916c888e22 100644
--- a/pkgs/development/python-modules/pydy/default.nix
+++ b/pkgs/development/python-modules/pydy/default.nix
@@ -2,7 +2,6 @@
   lib,
   buildPythonPackage,
   fetchPypi,
-  pythonOlder,
   numpy,
   scipy,
   sympy,
@@ -29,9 +28,6 @@ buildPythonPackage rec {
     sympy
   ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     nose
     cython
diff --git a/pkgs/development/python-modules/pypass/default.nix b/pkgs/development/python-modules/pypass/default.nix
index 3a2fc269ceda4..a13d4ea197209 100644
--- a/pkgs/development/python-modules/pypass/default.nix
+++ b/pkgs/development/python-modules/pypass/default.nix
@@ -55,8 +55,6 @@ buildPythonPackage rec {
     pexpect
   ];
 
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [ nose ];
 
   # Configuration so that the tests work
diff --git a/pkgs/development/python-modules/pytimeparse/default.nix b/pkgs/development/python-modules/pytimeparse/default.nix
index 819bc5875c6f1..03b0bc996cdfd 100644
--- a/pkgs/development/python-modules/pytimeparse/default.nix
+++ b/pkgs/development/python-modules/pytimeparse/default.nix
@@ -21,9 +21,6 @@ buildPythonPackage rec {
 
   build-system = [ setuptools ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [ nose ];
 
   pythonImportsCheck = [ "pytimeparse" ];
diff --git a/pkgs/development/python-modules/seqdiag/default.nix b/pkgs/development/python-modules/seqdiag/default.nix
index 79d575a9fa516..d205c2e986a3e 100644
--- a/pkgs/development/python-modules/seqdiag/default.nix
+++ b/pkgs/development/python-modules/seqdiag/default.nix
@@ -27,9 +27,6 @@ buildPythonPackage rec {
 
   dependencies = [ blockdiag ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     nose
     pytestCheckHook
diff --git a/pkgs/development/python-modules/sphinx-rtd-dark-mode/default.nix b/pkgs/development/python-modules/sphinx-rtd-dark-mode/default.nix
index d3b4bf7f41045..67d6794ee1d58 100644
--- a/pkgs/development/python-modules/sphinx-rtd-dark-mode/default.nix
+++ b/pkgs/development/python-modules/sphinx-rtd-dark-mode/default.nix
@@ -25,9 +25,6 @@ buildPythonPackage rec {
 
   dependencies = [ sphinx-rtd-theme ];
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     nose
     sphinx
diff --git a/pkgs/development/python-modules/uvcclient/default.nix b/pkgs/development/python-modules/uvcclient/default.nix
index c884e3a029261..374bbdde7a4e4 100644
--- a/pkgs/development/python-modules/uvcclient/default.nix
+++ b/pkgs/development/python-modules/uvcclient/default.nix
@@ -2,7 +2,6 @@
   lib,
   buildPythonPackage,
   fetchFromGitHub,
-  pythonOlder,
   nose,
   mock,
 }:
@@ -24,9 +23,6 @@ buildPythonPackage rec {
       --replace-fail "assertEquals" "assertEqual"
   '';
 
-  # tests rely on nose
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [
     nose
     mock
diff --git a/pkgs/development/python-modules/xlwt/default.nix b/pkgs/development/python-modules/xlwt/default.nix
index 12dfb75ce3410..e4f1821431298 100644
--- a/pkgs/development/python-modules/xlwt/default.nix
+++ b/pkgs/development/python-modules/xlwt/default.nix
@@ -21,9 +21,6 @@ buildPythonPackage rec {
 
   build-system = [ setuptools ];
 
-  # tests rely on nose, archived in 2020
-  doCheck = pythonOlder "3.12";
-
   nativeCheckInputs = [ nose ];
 
   checkPhase = ''