diff --git a/pkgs/by-name/ma/mackup/package.nix b/pkgs/by-name/ma/mackup/package.nix
index b39590b6454a2..1e75c0cefcb65 100644
--- a/pkgs/by-name/ma/mackup/package.nix
+++ b/pkgs/by-name/ma/mackup/package.nix
@@ -23,7 +23,6 @@ python3Packages.buildPythonApplication rec {
 
   nativeBuildInputs = with python3Packages; [
     poetry-core
-    pythonRelaxDepsHook
     nose
   ];
 
diff --git a/pkgs/by-name/mo/mov-cli/package.nix b/pkgs/by-name/mo/mov-cli/package.nix
index 46828bbb23b5c..1a00f4ba019ab 100644
--- a/pkgs/by-name/mo/mov-cli/package.nix
+++ b/pkgs/by-name/mo/mov-cli/package.nix
@@ -43,10 +43,6 @@ python3.pkgs.buildPythonPackage {
     (callPackage ./mov-cli-test.nix {})
   ];
 
-  nativeBuildInputs = [
-    python3.pkgs.pythonRelaxDepsHook
-  ];
-
   pythonRelaxDeps = [
     "httpx"
     "tldextract"
diff --git a/pkgs/by-name/sp/sploitscan/package.nix b/pkgs/by-name/sp/sploitscan/package.nix
index 183832f099120..aaf61456b9228 100644
--- a/pkgs/by-name/sp/sploitscan/package.nix
+++ b/pkgs/by-name/sp/sploitscan/package.nix
@@ -24,8 +24,6 @@ python3.pkgs.buildPythonApplication rec {
     setuptools
   ];
 
-  nativeBuildInputs = with python3.pkgs; [ pythonRelaxDepsHook ];
-
   dependencies = with python3.pkgs; [
     jinja2
     openai
diff --git a/pkgs/by-name/tg/tg-archive/package.nix b/pkgs/by-name/tg/tg-archive/package.nix
index 7fd336ecedece..4465fa35cbc75 100644
--- a/pkgs/by-name/tg/tg-archive/package.nix
+++ b/pkgs/by-name/tg/tg-archive/package.nix
@@ -20,10 +20,6 @@ in python3.pkgs.buildPythonApplication {
   pyproject = true;
   pythonRelaxDeps = true;
 
-  nativeBuildInputs = with python3.pkgs; [
-    pythonRelaxDepsHook
-  ];
-
   propagatedBuildInputs = with python3.pkgs; [
     setuptools
     telethon
diff --git a/pkgs/development/python-modules/bentoml/default.nix b/pkgs/development/python-modules/bentoml/default.nix
index ab5d4025dbf99..828021217c413 100644
--- a/pkgs/development/python-modules/bentoml/default.nix
+++ b/pkgs/development/python-modules/bentoml/default.nix
@@ -4,7 +4,6 @@
   buildPythonPackage,
   fetchFromGitHub,
   pythonOlder,
-  pythonRelaxDepsHook,
   hatchling,
   hatch-vcs,
   aiohttp,
@@ -130,8 +129,6 @@ buildPythonPackage {
     hash = "sha256-giZteSikwS9YEcVMPCC9h2khbBgvUPRW1biAyixO13Y=";
   };
 
-  nativeBuildInputs = [ pythonRelaxDepsHook ];
-
   pythonRelaxDeps = [
     "cattrs"
     "nvidia-ml-py"
diff --git a/pkgs/development/python-modules/hdate/default.nix b/pkgs/development/python-modules/hdate/default.nix
index 0b3fa2ab78a44..9fe99f93b30e5 100644
--- a/pkgs/development/python-modules/hdate/default.nix
+++ b/pkgs/development/python-modules/hdate/default.nix
@@ -4,7 +4,6 @@
   buildPythonPackage,
   fetchFromGitHub,
   pdm-backend,
-  pythonRelaxDepsHook,
   pytestCheckHook,
   pythonOlder,
   pytz,
diff --git a/pkgs/development/python-modules/llama-index/default.nix b/pkgs/development/python-modules/llama-index/default.nix
index 575c61c89f41f..97cfb265396d0 100644
--- a/pkgs/development/python-modules/llama-index/default.nix
+++ b/pkgs/development/python-modules/llama-index/default.nix
@@ -2,7 +2,6 @@
   lib,
   buildPythonPackage,
   poetry-core,
-  pythonRelaxDepsHook,
   llama-index-agent-openai,
   llama-index-cli,
   llama-index-core,
@@ -26,10 +25,6 @@ buildPythonPackage {
 
   build-system = [ poetry-core ];
 
-  nativeBuildInputs = [
-    pythonRelaxDepsHook
-  ];
-
   pythonRelaxDeps = [
     "llama-index-core"
   ];
diff --git a/pkgs/development/python-modules/rapidocr-onnxruntime/default.nix b/pkgs/development/python-modules/rapidocr-onnxruntime/default.nix
index 48209fc9efca3..aae91c7567e0b 100644
--- a/pkgs/development/python-modules/rapidocr-onnxruntime/default.nix
+++ b/pkgs/development/python-modules/rapidocr-onnxruntime/default.nix
@@ -6,7 +6,6 @@
 
   fetchzip,
   substitute,
-  pythonRelaxDepsHook,
   pytestCheckHook,
 
   setuptools,
@@ -73,8 +72,6 @@ buildPythonPackage {
     echo "from .rapidocr_onnxruntime.main import RapidOCR, VisRes" > __init__.py
   '';
 
-  nativeBuildInputs = [ pythonRelaxDepsHook ];
-
   # Upstream expects the source files to be under rapidocr_onnxruntime/rapidocr_onnxruntime
   # instead of rapidocr_onnxruntime for the wheel to build correctly.
   preBuild = ''
diff --git a/pkgs/development/python-modules/vcard/default.nix b/pkgs/development/python-modules/vcard/default.nix
index a2fe7abb3b3ba..11d8c496a7067 100644
--- a/pkgs/development/python-modules/vcard/default.nix
+++ b/pkgs/development/python-modules/vcard/default.nix
@@ -6,7 +6,6 @@
   python-dateutil,
   pythonAtLeast,
   pythonOlder,
-  pythonRelaxDepsHook,
   setuptools,
 }:
 
@@ -28,8 +27,6 @@ buildPythonPackage rec {
 
   build-system = [ setuptools ];
 
-  nativeBuildInputs = [ pythonRelaxDepsHook ];
-
   dependencies = [ python-dateutil ];
 
   nativeCheckInputs = [ pytestCheckHook ];
diff --git a/pkgs/development/tools/aws-sam-cli/default.nix b/pkgs/development/tools/aws-sam-cli/default.nix
index e94a98a6a96a8..9ad378db45e8f 100644
--- a/pkgs/development/tools/aws-sam-cli/default.nix
+++ b/pkgs/development/tools/aws-sam-cli/default.nix
@@ -26,10 +26,6 @@ python3.pkgs.buildPythonApplication rec {
     setuptools
   ];
 
-  nativeBuildInputs = with python3.pkgs; [
-    pythonRelaxDepsHook
-  ];
-
   pythonRelaxDeps = [
     "aws-lambda-builders"
     "aws-sam-translator"