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Error in Phase 4 of Semi-supervised Clustering #216

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alonzowolfram opened this issue Sep 12, 2024 · 1 comment
Open

Error in Phase 4 of Semi-supervised Clustering #216

alonzowolfram opened this issue Sep 12, 2024 · 1 comment

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@alonzowolfram
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Hello,

When running insitutype() on my data, I get the following error in phase 4:
phase 4: classifying all 405339 cells Error in .local(x, ...) : Cells with 0 counts were found. Please remove.

I have removed all the cells with 0 counts, and I still get this error. This is the code I'm using:

insitutype(
        x = counts_sub,
        neg = negmean_sub,
        cohort = metadata_sub$fastCohorting,
        bg = NULL,
        n_clusts = n_clusts_lower_bound:n_clusts_upper_bound,
        reference_profiles = updatedprofiles,
        update_reference_profiles = FALSE,
        n_phase1 = 10000,
        n_phase2 = 20000,
        n_phase3 = 1e+05,
        n_starts = 10,
        max_iters = 40
      )

I've double-checked all the input, and it looks all right, but maybe I'm missing something obvious? Thanks!

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