diff --git a/software/glasgow/device/hardware.py b/software/glasgow/device/hardware.py
index 49c4f97d3..0b799f67d 100644
--- a/software/glasgow/device/hardware.py
+++ b/software/glasgow/device/hardware.py
@@ -54,9 +54,23 @@ def __init__(self, context):
         self.context = context
 
     def run(self):
+        # The poller thread spends most of its life in blocking `handleEvents()` calls and this can
+        # cause issues during interpreter shutdown. If it were a daemon thread (it isn't) then it
+        # would be instantly killed on interpreter shutdown and any locks it took could block some
+        # libusb1 objects being destroyed as their Python counterparts are garbage collected. Since
+        # it is not a daemon thread, `threading._shutdown()` (that is called by e.g. `exit()`) will
+        # join it, and so it must terminate during threading shutdown. Note that `atexit.register`
+        # is not enough as those callbacks are called after threading shutdown, and past the point
+        # where a deadlock would happen.
+        threading._register_atexit(self.stop)
         while not self.done:
             self.context.handleEvents()
 
+    def stop(self):
+        self.done = True
+        self.context.interruptEventHandler()
+        self.join()
+
 
 class GlasgowHardwareDevice:
     @classmethod
@@ -228,8 +242,8 @@ def modified_design(self):
         return self._modified_design
 
     def close(self):
-        self.usb_poller.done = True
         self.usb_handle.close()
+        self.usb_poller.stop()
         self.usb_context.close()
 
     async def _do_transfer(self, is_read, setup):
diff --git a/software/pdm.min.lock b/software/pdm.min.lock
index 9c0a0c59b..c976105b0 100644
--- a/software/pdm.min.lock
+++ b/software/pdm.min.lock
@@ -5,7 +5,7 @@
 groups = ["default", "builtin-toolchain", "http"]
 strategy = ["cross_platform", "direct_minimal_versions"]
 lock_version = "4.4"
-content_hash = "sha256:8f3a6d83a8f959b68ed91a06346c34d47403afa5cb1c6f926db996addce63bb7"
+content_hash = "sha256:d8664d854e834393abda2400adb7cc1f1cdd037e7c4c22b497b237756f31243f"
 
 [[package]]
 name = "aiohttp"
@@ -269,13 +269,13 @@ files = [
 
 [[package]]
 name = "libusb1"
-version = "2.0.1"
+version = "3.1.0"
 summary = "Pure-python wrapper for libusb-1.0"
 files = [
-    {file = "libusb1-2.0.1-py3-none-any.whl", hash = "sha256:81381ce1d8852a4d4345b2ee8218971d35865b5f025fef96b43ee082757099cd"},
-    {file = "libusb1-2.0.1-py3-none-win32.whl", hash = "sha256:9fda3055c98ab043cfb3beac93ef1de2900ad11d949c694f58bdf414ce2bd03c"},
-    {file = "libusb1-2.0.1-py3-none-win_amd64.whl", hash = "sha256:a97bcb90f589d863c5e971b013c8cf7e1915680a951e66c4222a2c5bb64b7153"},
-    {file = "libusb1-2.0.1.tar.gz", hash = "sha256:d3ba82ecf7ab6a48d21dac6697e26504670cc3522b8e5941bd28fb56cf3f6c46"},
+    {file = "libusb1-3.1.0-py3-none-any.whl", hash = "sha256:9d9f16e2c199cab91f48ead585d3f5ec7e8e4be428a25ddfed22abf786fa9b3a"},
+    {file = "libusb1-3.1.0-py3-none-win32.whl", hash = "sha256:bc7874302565721f443a27d8182fcc7152e5b560523f12f1377b130f473e4a0c"},
+    {file = "libusb1-3.1.0-py3-none-win_amd64.whl", hash = "sha256:77a06ecfb3d002d7c2ce369e28d0138b292fe8db8a3d102b73fda231a716dd35"},
+    {file = "libusb1-3.1.0.tar.gz", hash = "sha256:4ee9b0a55f8bd0b3ea7017ae919a6c1f439af742c4a4b04543c5fd7af89b828c"},
 ]
 
 [[package]]
diff --git a/software/pyproject.toml b/software/pyproject.toml
index e54699586..fcaab3a85 100644
--- a/software/pyproject.toml
+++ b/software/pyproject.toml
@@ -46,7 +46,7 @@ dependencies = [
   # `libusb1` is used to communicate with the device, and its API mirrors the stable API/ABI of
   # of the native `libusb1` library. It increases major version when dropping support for older
   # Python versions.
-  "libusb1>=2.0.1",
+  "libusb1>=3.1.0",
   # `pyvcd` is used in the applet analyzer to dump waveform files. It is also a dependency of
   # Amaranth, and the version range here must be compatible with Amaranth's.
   "pyvcd>=0.2,<0.5",
@@ -54,13 +54,13 @@ dependencies = [
 
 [project.optional-dependencies]
 # By default, Glasgow uses the YoWASP (https://yowasp.org/) to build bitstreams. YoWASP is not
-# installable on certain architectures and platforms, so this dependency is optional
+# installable on certain architectures and platforms, so this dependency is optional.
 builtin-toolchain = [
   # The version of `amaranth-yosys` is constrained by Amaranth; this optional dependency only
   # includes it in the dependency resolution set.
   "amaranth-yosys",
   # `yowasp-runtime` is a dependency of other toolchain components, and it is constrained here to
-  # the lowest that has features required for isolated builds.
+  # the lowest that has features transitively required by Glasgow.
   "yowasp-runtime>=1.42",
   # Most versions of Yosys and nextpnr work; the minimum versions listed here are known good.
   "yowasp-yosys>=0.31.0.13",