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docpost
CLIMB-COVID Data Changelog
2021-04-22 14:00:00 +0000
2021-05-14 17:45:00 +0000
samstudio8
samstudio8
assets/dipi-patch.png
CLIMB-COVID DIPI Mission Patch

All notable changes to CLIMB-COVID APIs, data or interchange formats that have impact to users or other pipelines should be documented in this file. Changes described here may only be a subset of all changes to a project as this log concerns itself only with changes that impact how data is provided or consumed by users or other pipelines. The following DIPI projects are routinely using this CHANGELOG.

  • Majora API -- metadata APIs
  • Ocarina -- Majora command line client (Full changelog)
  • Elan -- inbound data pipeline
  • Tael -- MQTT messaging tools
  • Asklepian -- Outbound PHE pipeline
  • CLIMB-COVID -- metaprojects (eg. status page, data page)
  • Foel -- second generation CLIMB-COVID ingest system

The format is based on Keep a Changelog.

2022-03-03

Elan

  • Elan v2 supersedes Elan v1; all data processed today and onward will pass through Elan v2

2022-02-23

Majora Magenta

  • Effective immediately, "Test Majora", lovingly known as "Majora Magenta" has moved URL to https://majora-test.covid19.climb.ac.uk/.
    • The test database has not been migrated and users will need to register new accounts and new OAuth applications.

Datapipe

Changed

  • An additional column ambiguities providing a pipe separated list of ambiguous regions has been added to metadata outputs including mutations.

2022-02-18

Elan

Changed

  • The location of individual BAM and BAI files are being migrated over the weekend. While all care is being taken to minimise impact to users, attempts to access individual BAM and BAI files may fail over this weekend period.

2022-02-04

Foel

Changed

  • Foel will now reject BAM submissions if the BAM file is larger than 2 GB (defined as 2e9 bytes)

2022-02-01

Elan

Removed

  • The "individual FASTA" directory at /bham/artifacts/published/fasta has been removed
  • The "individual BAM" directory at /bham/artifacts/published/alignment has been removed

2022-01-28

Elan

Removed

  • New FASTA files are no longer linked in /cephfs/covid/bham/artifacts/published/fasta
    • Users should extract their sequences directly from the daily consensus FASTA (elan.consensus.fasta), leveraging its index. A sequence extraction utility (seq_extract) is available via our utilities repository.
  • New BAM files are no longer linked in /cephfs/covid/bham/artifacts/published/alignment
    • Users must now resolve the location of BAM files using /cephfs/covid/artifacts/elan/latest/majora.pag_lookup.tsv
  • As a side effect of an update to the publishing pipeline, the /cephfs/covid/bham/artifacts/published/20220128 directory was accidentally removed. As we are working towards removing these directories anyway, the change will remain and these "dated artifact" directories will no longer be published.
    • Users can continue to use /cephfs/covid/bham/artifacts/published/latest which will contain the latest artifacts to maintain compatibility

Changed

  • The files detailing which samples were missing and removed by Elan have moved from /cephfs/covid/bham/artifacts/published/latest/summary to /cephfs/covid/artifacts/elan/latest/:
    • Use elan.missing.ls for determining why samples were not ingested by Elan (missing metadata or missing files)
    • Use elan.quickcheck.ls for samples rejected by Elan screening (invalid FASTA or BAM)

2022-01-25

Datapipe

Changed

  • Created a separate nextflow for geography cleaning steps, moving them earlier in the pipeline. Results should be unaffected.

Elan

Changed

  • /cephfs/covid/artifacts is the new top-level home for artifacts
    • Moving the location of the artifacts is necessary for longer term maintenance of the CLIMB-COVID project and additionally allows us to work toward solving the infamous "big dir" problem.
  • The FASTA header for the daily elan.consensus.fasta will no longer contain the pipe delimited "row number" and will now only contain the Published Artifact Group (PAG) name. The FAI index will therefore only contain the PAG names, making it easier to maintain random access to sequences. Pipe delimited metadata may be added again in future as a sequence comment, rather than as part of the sequence header.
    • Python code using seq_name.split('|')[0] to parse this header will be unaffected, as the split will succeed, but developers can now just use seq_name.

Added

  • /cephfs/covid/artifacts/elan/latest/majora.pag_lookup.tsv now allows users to write scripts to look up (central_sample_id AND run_name) tuples, OR pag_name to resolve the locations of published BAMs

Deprecated

  • The individual FASTA and BAM directories at /bham/artifacts/published/fasta and /bham/artifacts/published/alignment will be removed without exception on 2022-01-31. These directories contain hundreds of thousands of symlinks and need to be removed as part of our solution to the "big dir" problem.
    • FASTA users: As per previous guidance, users should extract their sequences directly from the daily consensus FASTA (elan.consensus.fasta), leveraging its index. For users unsure how to do this effectively, we have now made a sequence extraction utility (seq_extract) available via our utilities repository.
    • BAM users: Users must now resolve the location of BAM files using the new majora.pag_lookup.tsv, first published today

Majora, Ocarina

Changed

  • The minimum Ocarina client version for Majora to accept requests has been bumped to v0.44.0. Requests from clients below this version number will be rejected immediately.

2022-01-24

Elan

Deprecated

  • After consultation, the "individual QC" outputs are now considered deprecated and will be removed without warning in the near future. A new API service will allow users to fetch QC information as an alternative.
  • The "individual FASTA" outputs are now considered deprecated and will be removed without warning in the immediate future. Users should follow our recommendation and ensure they are using the daily consensus FASTA and the corresponding FAI index to query for sequences as an alternative.

2022-01-13

Elan

Changed

  • Elan will now add files, metrics and QC reports to Majora as service-elan, not nicholsz

Foel

Changed

  • Foel will now add empty biosamples, libraries and sequencing runs to Majora as service-foel, not nicholsz

2022-01-06

Elan

Changed

  • Elan will refuse to process FASTA files containing one or more non-IUPAC characters or -

2021-12-08

Majora

Changed

  • api.artifact.biosample.addempty supports using an optional metadata parameter to add key value metadata to empty biosamples

2021-12-03

Foel

Added

  • sample_route is now a required field in the metadata CSV

Changed

  • The minimal csv_template_version has been increased to 2 to coincide with the new sample_route field. Submissions using csv_template_version 1 will be rejected.

CLIMB-COVID

Changed

  • Automated GISAID submissions are now made every day, with a seven day lag

2021-11-03

Majora

Changed

  • INVITROGEN added to test_kit ct validator

datapipe

Changed

  • Substitute 'N' for '?' in input FASTA. (continue otherwise to reject all FASTA input sequences with non-IUPAC characters)

2021-10-29

datapipe

Changed

  • Add flag --score-N=0 to minimap2 command as part of MSA building step
  • Reject all FASTA input sequences with non-IUPAC characters

2021-10-23

datapipe

Changed

  • Temporary fix of replacing ? characters in incoming FASTA with Ns
  • Add further parallelization and line count checks to handle file corruptions

2021-10-14

phylopipe

Changed

  • Attempted switch back to big tree - one off tree published, minus the microreact outputs

2021-09-21

phylopipe

Changed

  • Ran with refreshed base tree (~475k tips down from 1.4m tips)

2021-09-23

Majora

Fixed

  • Majora will now correctly reject biosamples with a collection_date after the received_date with an error message: "Sample cannot be collected after it was received. Perhaps they have been swapped?"

2021-09-21

datapipe

Changed

  • Added gzipped output files to s3 bucket

2021-09-01

datapipe

Changed

  • Updated memory requirements for publish steps
  • Parallelized minimap2 and alignment steps

2021-08-18

CLIMB-COVID

Changed

  • The head node has moved to different hardware. SSH users and sequence uploaders should be aware that the ESDCA key has been changed.
    • Users should not be alarmed by the rather alarming message (below) that will be issued on the command line the first time they attempt to log in after this change:
    @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
@    WARNING: REMOTE HOST IDENTIFICATION HAS CHANGED!     @
@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
IT IS POSSIBLE THAT SOMEONE IS DOING SOMETHING NASTY!
    • Follow the instructions in the error message to remove the offending keys from your known_hosts file. The line numbers to remove will be listed in the message, for example this message would indicate you must remove line 8 from your known_hosts file:
    Offending key for IP in /home/user/.ssh/known_hosts:8 <= number after the colon is the line number, this is just an example

2021-08-11

Ocarina

Changed

  • Ocarina 0.43.0 now allows OAuth authentication for pag suppress, users will need to update to 0.43.0 for the new configuration to work.
  • Ocarina 0.42.1 uses exit codes as suggested by BSD sysexits. This may affect users catching particular non-zero exit codes from the Ocarina application.

2021-08-05

Asklepian

Changed

  • To improve performance, the MSA generation algorithm will be swapped from datafunk to gofasta.
  • Users should be aware (but not concerned) of a small impact to the integrity of 14 sequences in the MSA (and downstream Asklepian tables).

2021-07-17

Majora

Changed

  • Majora will no longer accept a collection_date or received_date for a biosample where the year is not 2020 or higher, regardless of whether the biosample is being added or updated

Tael

Changed

  • mqtt-message automatically adds ts key to payloads, containing the UNIX epoch time
    • Recommended that users no longer use the date and time fields for anything other than human readability

2021-07-13

datapipe

Changed

  • Now runs gofasta updown list and creates output files for CIVET3

2021-07-07

datapipe

Changed

  • Adds a metadata output to cog/UTLA_genome_counts_<date>.csv
  • Excludes samples labelled to omit when the why_excluded column is not in published metadata output to avoid mysterious duplicate rows

2021-06-21

CLIMB-COVID

Changed

  • Files and directories in user upload dirs (climb-covid19-user/upload) will be periodically scanned and deleted if they are more than two weeks old.
    • This first run on June 21st will remove data older than June 7th

2021-06-10

Majora

Changed

  • Users with the force_add_biosampleartifact scope can now add sender_sample_id to blank biosamples created through the biosample.addempty endpoint (Majora biosample.addempty docs), regardless of whether the sample was previously added by addempty before
    • This does not change existing behaviour that prevents samples that have been "filled in" with full metadata via biosample.add from being modified by biosample.addempty

2021-06-01

Datapipe

Changed

  • Started performing geography cleaning of all adm1s (largely global cleaning)

Majora

Changed

  • Users with the force_add_biosampleartifact scope can now add sender_sample_id to blank biosamples created through the biosample.addempty endpoint (Majora biosample.addempty docs)
  • Existing biosample artifacts that were collected and/or received more than a year ago can now be updated through the metadata uploader, or Ocarina (without --partial) as the past-date checks are now skipped for existing data
    • This does not change the behaviour of collection_date or received_date from over 365 days ago being rejected for new samples
    • collection_date and received_date set to a future date are still rejected regardless of whether the sample exists or not

Ocarina

Changed

  • Ocarina 0.40.2 ocarina empty biosample now takes an additional --sender-sample-id option (Ocarina Changelog)

Phylopipe2.0

Changed

  • Makes civet output directories identical to previous except for explicitly containing "public" or "private" in file names
  • Adds additional masking before usher updates at sites which are known to cause usher problems placing on a tree
  • Fixed phylotyping bug (change to clusterfunk)
  • Will be published to latest and today's phylopipe1 output when it completes in 2 days time will be published to old

2021-05-31

Datapipe

Changed

  • New columns added to metadata outputs in line with pangolin changes are now all populated
  • The pillar_2 column has been renamed is_pillar_2 and contains Y/N for consistency with incoming pipeline
  • Bug fixes adding cleanded geography to metadata
  • Additional requested columns/metadata outputs published

2021-05-27

Pangolin

Changed

  • Major release of pangolin v3.0 with downstream changes in datapipe, phylopipe, pathogenwatch expected in coming days
  • Pangolin output now includes a new version column containing information about inference engine (pangoLEARN, usher or designation hash) and data release on which assignments were based, to be used instead of pangoLEARN_version column
  • Pangolin outputs constellation calls made by scorpio for a number of VOCs/VUIs

2021-05-18

CLIMB-COVID

Added

2021-05-14

Tael

Fixed

  • Patched a regression in mqtt-client.py that caused clients not requiring any environment variables (--envreq) to silently fail to start their specified command
    • Clients not specifying --envreq should be restarted as soon as possible

Phylopipe

Added

  • Beta release of phylopipe2.0 published daily

2021-05-02

Tael

Added

  • mqtt-client.py automatically subscribes clients to a "control topic" named COGUK/infrastructure/pipelines/<who>/control, where who is the name of the pipeline provided to --who
  • Pipelines can now be manually raised by sending messages to the client control topic, specfying an action key with the value of raise

Changed

  • The started message emitted by mqtt-client.py now includes a reason key, explaining why the pipeline has started

2021-04-30

Datapipe

Changed

  • Updated minimap2 sequence divergence threshold preset from 5% to 20% divergence as increasing numbers of (primarily GISAID) sequences were being lost from the MSA
  • Passes uk_lineage metadata column through from previous datapipe run for use by phylopipe

2021-04-23

Asklepian

Changed

2021-04-21

Asklepian

Added

Changed

2021-04-16

CLIMB-COVID

Added

2021-04-13

Tael

Added

  • Payload keys targeted by --envreq are copied through to the output payload if --envprefix has not been provided. --envprefix still copies all payload keys to the output with the specified prefix.

Changed

  • Payload keys that will be passed through to the finished output payload will also be emitted in the start ouput payload

2021-04-12

Majora API

Changed

Tael

Added

  • --payload-passthrough parameter allows keys from the input payload to be automatically copied to the finished output payload

2021-04-01

Elan

Changed

  • Elan will no longer sort uploaded BAMs. User guidance has always been that BAMs should be sorted before uploaded so this step is a waste of compute resources and time. Unsorted BAMs will now be considered invalid and rejected by Elan as part of the quickcheck report.

2021-03-18

Majora API

Changed

2021-03-06

DIPI

Added

  • Introduced this CHANGELOG to track notable changes to data and pipeline interchange formats. Significant changes before this date are out of the scope of this CHANGELOG.

Elan

Changed